Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,150,407 G→T 57.7% P280P (CCG→CCT)  yceG → septation protein, ampicillin sensitivity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,150,4070GT57.7% 33.5 / inf 293P280P (CCG→CCT) yceGseptation protein, ampicillin sensitivity
Reads supporting (aligned to +/- strand):  ref base G (74/50);  new base T (84/84);  total (159/134)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 304 total aligned reads displayed.

GTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAGTCTGTGCAGGAT  >  CP009273/1150258‑1150554
                                                                                                                                                     |                                                                                                                                                   
 tATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTgccgc                                                                                                                                                    >  2:2443585/1‑150 (MQ=255)
      aTCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGt                                                                                                                                               >  2:3349752/1‑150 (MQ=255)
      aTCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGt                                                                                                                                               >  1:1443923/1‑150 (MQ=255)
       tCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTg                                                                                                                                              <  1:805508/150‑1 (MQ=255)
        cAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGc                                                                                                                                             <  2:1980811/150‑1 (MQ=255)
          aCCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGa                                                                                                                                           >  2:4600237/1‑150 (MQ=255)
           ccGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGAt                                                                                                                                          >  1:4592657/1‑150 (MQ=255)
           ccGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGAt                                                                                                                                          >  1:2258205/1‑150 (MQ=255)
             gTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAg                                                                                                                                        >  1:2358640/1‑150 (MQ=255)
               ttACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCg                                                                                                                                      >  1:2036880/1‑150 (MQ=255)
                 aCGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGAc                                                                                                                                    <  1:1345141/150‑1 (MQ=255)
                   gCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGc                                                                                                                                  >  1:4552154/1‑150 (MQ=255)
                    cATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGcc                                                                                                                                 >  1:1275865/1‑150 (MQ=255)
                    cATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGcc                                                                                                                                 <  2:2496368/150‑1 (MQ=255)
                    cATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGcc                                                                                                                                 <  1:595070/150‑1 (MQ=255)
                        ggTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCgggg                                                                                                                             >  1:810716/1‑150 (MQ=255)
                          tATGCGCCTGCAGACCGACCCTTCGGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGTAATCGCCGACCGCGTATAACACCTATACCATTACCGTTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGc                                                                                                                           <  2:3176727/150‑1 (MQ=255)
                          tATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGc                                                                                                                           <  1:3177143/150‑1 (MQ=255)
                          tATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGc                                                                                                                           <  2:3636251/150‑1 (MQ=255)
                          tATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGc                                                                                                                           >  2:3779915/1‑150 (MQ=255)
                          tATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGc                                                                                                                           >  2:4012235/1‑150 (MQ=255)
                          tATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGc                                                                                                                           >  2:4365138/1‑150 (MQ=255)
                               gcCTGCAGACCGCCCCGCCCGTGATTTACGGGATGGGAGAGCGTAATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGAtt                                                                                                                      <  2:822422/150‑1 (MQ=255)
                                   gCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCt                                                                                                                  <  2:1620140/150‑1 (MQ=255)
                                     aGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGa                                                                                                                >  2:1385737/1‑150 (MQ=255)
                                       accgaccCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAg                                                                                                              >  2:4297073/1‑150 (MQ=255)
                                         cgacccgaccGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGgc                                                                                                            <  1:2421433/150‑1 (MQ=255)
                                         cgacccgaccGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGgc                                                                                                            >  1:1951735/1‑150 (MQ=255)
                                          gacccgaccGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGgct                                                                                                           <  1:1808189/150‑1 (MQ=255)
                                           acccgaccGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGgctg                                                                                                          >  2:2991883/1‑150 (MQ=255)
                                              cgaccGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGctgctg                                                                                                       >  2:3121571/1‑150 (MQ=255)
                                                accGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTgcg                                                                                                     <  2:2157500/150‑1 (MQ=255)
                                                accGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTgcg                                                                                                     >  2:1972603/1‑150 (MQ=255)
                                                  cGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCa                                                                                                   >  2:2804339/1‑150 (MQ=255)
                                                     gATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATcc                                                                                                >  1:1957618/1‑150 (MQ=255)
                                                      aTTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCg                                                                                               <  1:712377/150‑1 (MQ=255)
                                                             ggATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCACCAGGTGCGATATCTAATCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCTGCaaaaa                                                                                        >  2:4150535/1‑150 (MQ=255)
                                                               aTGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACg                                                                                      >  1:3756201/1‑150 (MQ=255)
                                                                 gggAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGcc                                                                                    <  2:1919070/150‑1 (MQ=255)
                                                                   gagaGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGt                                                                                  <  2:1260058/150‑1 (MQ=255)
                                                                     gagCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTAt                                                                                <  1:3461131/150‑1 (MQ=255)
                                                                      agCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACAGGTCTGCCTCCAGGTGCGATAGCGACGACGGTGGCTGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTAtc                                                                               >  2:3292300/1‑150 (MQ=255)
                                                                       gCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTAtct                                                                              <  2:3509965/150‑1 (MQ=255)
                                                                        cGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTAtctc                                                                             >  2:728048/1‑150 (MQ=255)
                                                                          ttATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTa                                                                           <  2:570257/150‑1 (MQ=255)
                                                                           tataATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTAt                                                                          <  2:661151/150‑1 (MQ=255)
                                                                           tataATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTAt                                                                          <  1:3890983/150‑1 (MQ=255)
                                                                            ataATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTAtt                                                                         >  1:421328/1‑150 (MQ=255)
                                                                             ttctGGCAAACTTTCTAGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGTTCTGCCGCCAGGTGCGATAGCGACGACGGGGGCGGATTCGCTGTAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTAttt                                                                        <  2:4041038/147‑1 (MQ=255)
                                                                              aaTGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTAtttt                                                                       >  2:3056752/1‑150 (MQ=255)
                                                                                   cAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGc                                                                  >  2:1545805/1‑150 (MQ=255)
                                                                                   cAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGc                                                                  <  2:1752795/150‑1 (MQ=255)
                                                                                    aaaCTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGcc                                                                 <  2:1007720/150‑1 (MQ=255)
                                                                                     aaCTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCg                                                                <  1:1229199/150‑1 (MQ=255)
                                                                                      aCTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGa                                                               >  2:384996/1‑150 (MQ=255)
                                                                                           ctcGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATAGCATTACCGGTCTGCCGCCAGGTGCGTTAGCGACGCCGGGGGAGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTa                                                          <  1:1075026/150‑1 (MQ=255)
                                                                                                gCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAggt                                                     >  2:4635563/1‑150 (MQ=255)
                                                                                                gCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAggt                                                     <  1:1878064/150‑1 (MQ=255)
                                                                                                 cAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAggtg                                                    <  2:2631305/150‑1 (MQ=255)
                                                                                                  aGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAggtgg                                                   >  1:2704400/1‑150 (MQ=255)
                                                                                                    aCCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTc                                                 <  1:4394802/150‑1 (MQ=255)
                                                                                                    aCCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTc                                                 <  1:447987/150‑1 (MQ=255)
                                                                                                     ccTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTca                                                >  1:2836585/1‑150 (MQ=255)
                                                                                                       tGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTcaca                                              >  2:3906008/1‑150 (MQ=255)
                                                                                                        ggAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCacac                                             >  2:1859859/1‑150 (MQ=255)
                                                                                                        ggAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCacac                                             <  1:2443585/150‑1 (MQ=255)
                                                                                                         gAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACg                                            <  1:485173/150‑1 (MQ=255)
                                                                                                         gAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACg                                            <  1:3484407/150‑1 (MQ=255)
                                                                                                          aaaCGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGt                                           >  2:3888408/1‑150 (MQ=255)
                                                                                                            aCGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGttt                                         <  2:1555986/150‑1 (MQ=255)
                                                                                                             cGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTa                                        <  1:3121571/150‑1 (MQ=255)
                                                                                                             cGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTa                                        <  1:1196171/150‑1 (MQ=255)
                                                                                                              gCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTaa                                       >  2:1288981/1‑150 (MQ=255)
                                                                                                               ccGACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAAt                                      <  2:2316407/150‑1 (MQ=255)
                                                                                                                 gACAGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATAc                                    >  2:1221393/1‑150 (MQ=255)
                                                                                                                    aGCGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCaa                                 >  1:4256713/1‑150 (MQ=255)
                                                                                                                      cGTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATc                               >  2:3607823/1‑150 (MQ=255)
                                                                                                                      cGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATc                               <  1:684409/150‑1 (MQ=255)
                                                                                                                      cGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATc                               <  2:3341977/150‑1 (MQ=255)
                                                                                                                       gTATAACACCTATACCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCt                              >  1:103073/1‑150 (MQ=255)
                                                                                                                       gTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCt                              <  2:1792571/150‑1 (MQ=255)
                                                                                                                       gTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCt                              <  2:1443900/150‑1 (MQ=255)
                                                                                                                         ataACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTg                            <  1:3658996/150‑1 (MQ=255)
                                                                                                                            acacCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCa                         <  1:2566562/150‑1 (MQ=255)
                                                                                                                              acCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGt                       >  1:2979972/1‑150 (MQ=255)
                                                                                                                               ccTCTACCAGTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTc                      <  2:4102248/150‑1 (MQ=255)
                                                                                                                               ccTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTc                      >  2:1346429/1‑150 (MQ=255)
                                                                                                                                  ataCCATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATa                   >  1:1534418/1‑150 (MQ=255)
                                                                                                                                   taCCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATaa                  <  1:3962483/150‑1 (MQ=255)
                                                                                                                                    aCCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAAc                 >  2:2126526/1‑150 (MQ=255)
                                                                                                                                    aCCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAAc                 <  1:1972724/150‑1 (MQ=255)
                                                                                                                                     ccATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACa                <  2:1479771/150‑1 (MQ=255)
                                                                                                                                      cATTACCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACaa               >  2:3693247/1‑150 (MQ=255)
                                                                                                                                         tACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAGTc            >  2:2227748/1‑150 (MQ=255)
                                                                                                                                          aCCGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAGTCt           <  2:3260121/150‑1 (MQ=255)
                                                                                                                                            cGGTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAGTCtgt         <  2:810629/150‑1 (MQ=255)
                                                                                                                                             ggTCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAGTCtgtg        >  1:1403060/1‑150 (MQ=255)
                                                                                                                                               tCTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAGTCTGTGCa      >  1:1582215/1‑150 (MQ=255)
                                                                                                                                                cTGCCTCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAGTCTGTGCAg     >  2:4424921/1‑150 (MQ=255)
                                                                                                                                                  gcctccAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAGTCTGTGCAGGa   >  1:3199934/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGATTCGCTGAAGGCTGCTGCGCATCCGGCAAAAACGCCGTATCTCTATTTTGTGGCCGATGGTAAAGGTGGTCACACGTTTAATACCAATCTTGCCAGTCATAACAAGTCTGTGCAGGAT  >  CP009273/1150258‑1150554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑