Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 103,467 G→T 100% D142Y (GAT→TAT)  lpxC → UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273103,4670GT98.8% 847.7 / 1.4 249D142Y (GAT→TAT) lpxCUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (111/135);  total (112/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 251 total aligned reads displayed.

GTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCGATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCAGATCAGCCGTGCGC  >  CP009273/103320‑103611
                                                                                                                                                   |                                                                                                                                                
gTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTAt                                                                                                                                                >  2:2219452/1‑150 (MQ=255)
 ttATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCtata                                                                                                                                               <  2:4416775/150‑1 (MQ=255)
  tATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATaa                                                                                                                                              >  2:1721307/1‑150 (MQ=255)
   aTCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAg                                                                                                                                             <  2:3514031/150‑1 (MQ=255)
     cGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTg                                                                                                                                           <  2:796032/150‑1 (MQ=255)
      gAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTgg                                                                                                                                          <  2:2119556/150‑1 (MQ=255)
      gAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTgg                                                                                                                                          >  2:2330556/1‑150 (MQ=255)
      gAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTgg                                                                                                                                          >  2:4196883/1‑150 (MQ=255)
       aaGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTggg                                                                                                                                         <  1:2341087/150‑1 (MQ=255)
           tAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGa                                                                                                                                     >  2:3324059/1‑150 (MQ=255)
           tAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGa                                                                                                                                     <  1:4582054/150‑1 (MQ=255)
           tAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGa                                                                                                                                     <  1:252834/150‑1 (MQ=255)
            aaCGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGaa                                                                                                                                    >  1:2201800/1‑150 (MQ=255)
              cgcgCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAAtt                                                                                                                                  >  1:637458/1‑150 (MQ=255)
                cgcCGGAAATCGCGATCATGGTCGGCAGCGCCGCTCCGTTTGTATTCCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTa                                                                                                                                <  1:4292418/150‑1 (MQ=255)
                cgcCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTa                                                                                                                                <  1:3095873/150‑1 (MQ=255)
                 gcCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTaa                                                                                                                               >  2:3139427/1‑150 (MQ=255)
                  ccGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAg                                                                                                                              <  1:2690926/150‑1 (MQ=255)
                   cGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGc                                                                                                                             >  1:3391129/1‑150 (MQ=255)
                    ggAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGcc                                                                                                                            >  1:900633/1‑150 (MQ=255)
                    ggAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGcc                                                                                                                            >  1:381134/1‑150 (MQ=255)
                    ggAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGcc                                                                                                                            >  1:3498234/1‑150 (MQ=255)
                     gAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCg                                                                                                                           >  1:3772749/1‑150 (MQ=255)
                       aaTCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTa                                                                                                                         <  1:2255908/150‑1 (MQ=255)
                             gATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCGTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATgg                                                                                                                   >  1:96862/1‑150 (MQ=255)
                              aTCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGt                                                                                                                  <  2:2263000/150‑1 (MQ=255)
                               tCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAGGTGGGCTGAATTTAAGCCGTACAATGGtt                                                                                                                 <  1:3556343/150‑1 (MQ=255)
                               tCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGtt                                                                                                                 <  2:627291/150‑1 (MQ=255)
                               tCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGtt                                                                                                                 <  1:1568023/150‑1 (MQ=255)
                               tCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGtt                                                                                                                 >  2:221726/1‑150 (MQ=255)
                                 aTGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGtttt                                                                                                               <  2:477034/150‑1 (MQ=255)
                                  tGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGttttt                                                                                                              <  1:1189791/150‑1 (MQ=255)
                                   ggACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTc                                                                                                             <  1:3300552/150‑1 (MQ=255)
                                    gACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCg                                                                                                            >  1:3622469/1‑150 (MQ=255)
                                       ggCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTg                                                                                                         >  1:2977392/1‑150 (MQ=255)
                                       ggCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTg                                                                                                         >  2:2580094/1‑150 (MQ=255)
                                          aGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGAt                                                                                                      >  1:3350376/1‑150 (MQ=255)
                                           gcgcCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGAtt                                                                                                     <  2:2295353/150‑1 (MQ=255)
                                             gccgcTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTc                                                                                                   <  2:1131354/150‑1 (MQ=255)
                                               cgcTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCAc                                                                                                 >  1:1282482/1‑150 (MQ=255)
                                                  tCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCAt                                                                                              >  2:3447631/1‑150 (MQ=255)
                                                    cGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCg                                                                                            >  1:1454881/1‑150 (MQ=255)
                                                       ttGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGAtt                                                                                         <  1:1509155/150‑1 (MQ=255)
                                                            taCCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAAc                                                                                    >  2:1485744/1‑150 (MQ=255)
                                                              ccTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCa                                                                                  <  1:1425684/150‑1 (MQ=255)
                                                              ccTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCa                                                                                  >  1:2052688/1‑150 (MQ=255)
                                                                tgctgcTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATc                                                                                <  2:779954/150‑1 (MQ=255)
                                                                tgctgcTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATc                                                                                <  2:2463941/150‑1 (MQ=255)
                                                                 gctgctTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATATCACCATCGATTTTAACCATcc                                                                               <  1:3379539/150‑1 (MQ=255)
                                                                  ctgctTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCg                                                                              >  2:3851817/1‑150 (MQ=255)
                                                                        gtcGCCGGTATCGACGAGTTGCACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGTCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGATGGATTTCACCATCGATTTTAACCATCCGGCTAtt                                                                        <  1:1157957/148‑1 (MQ=255)
                                                                        gACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTAtt                                                                        <  1:3860470/150‑1 (MQ=255)
                                                                           gCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGAt                                                                     <  1:558870/150‑1 (MQ=255)
                                                                            ccGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGAtt                                                                    >  2:3835881/1‑150 (MQ=255)
                                                                               gTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCa                                                                 >  1:852124/1‑150 (MQ=255)
                                                                               gTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCa                                                                 <  2:537948/150‑1 (MQ=255)
                                                                                 aTCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGCTTTCACCATCGATTTTAACAATCCGGCTATTGATTCCAga                                                               >  2:1987380/1‑149 (MQ=255)
                                                                                 aTCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGc                                                               <  1:3987260/150‑1 (MQ=255)
                                                                                  tCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCa                                                              <  1:4376425/150‑1 (MQ=255)
                                                                                    gacgaGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAAc                                                            >  2:1857295/1‑150 (MQ=255)
                                                                                     acgaGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAAcc                                                           <  1:263891/150‑1 (MQ=255)
                                                                                       gaGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAg                                                         >  1:3553745/1‑150 (MQ=255)
                                                                                       gaGCTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAg                                                         <  1:1442101/150‑1 (MQ=255)
                                                                                        aGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAgc                                                        >  1:467625/1‑150 (MQ=255)
                                                                                         gTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAgcg                                                       >  2:1666207/1‑150 (MQ=255)
                                                                                          ttGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAgcgc                                                      <  1:2790355/150‑1 (MQ=255)
                                                                                            gAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTa                                                    >  2:3731833/1‑150 (MQ=255)
                                                                                            gAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTa                                                    >  1:2484801/1‑150 (MQ=255)
                                                                                                tGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCg                                                >  2:1827438/1‑150 (MQ=255)
                                                                                                tGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCg                                                >  1:3134909/1‑150 (MQ=255)
                                                                                                  cgcCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGAt                                              <  1:4491797/150‑1 (MQ=255)
                                                                                                   gcCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATg                                             <  2:2911895/150‑1 (MQ=255)
                                                                                                       aaaaaTTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACt                                         >  1:3254598/1‑150 (MQ=255)
                                                                                                         aaaTTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTtc                                       <  1:4243671/150‑1 (MQ=255)
                                                                                                         aaaTTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTtc                                       >  2:2808205/1‑150 (MQ=255)
                                                                                                          aaTTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTtct                                      <  1:3234639/150‑1 (MQ=255)
                                                                                                          aaTTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTtct                                      <  1:1628655/150‑1 (MQ=255)
                                                                                                             ttGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCg                                   >  2:4476594/1‑150 (MQ=255)
                                                                                                             ttGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCg                                   <  1:4169131/150‑1 (MQ=255)
                                                                                                              tGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGc                                  >  1:1171751/1‑150 (MQ=255)
                                                                                                                  cGCATCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGAt                              >  2:3177524/1‑150 (MQ=255)
                                                                                                                      tCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGt                          <  2:3341685/150‑1 (MQ=255)
                                                                                                                      tCAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGt                          >  2:4114527/1‑150 (MQ=255)
                                                                                                                       cAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACACTGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGtt                         >  2:2687985/1‑150 (MQ=255)
                                                                                                                       cAAAGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGtt                         >  2:951100/1‑150 (MQ=255)
                                                                                                                         aaGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTa                       >  1:257948/1‑150 (MQ=255)
                                                                                                                         aaGAGACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTa                       <  1:3277954/150‑1 (MQ=255)
                                                                                                                          agagACTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTAt                      <  2:4638420/150‑1 (MQ=255)
                                                                                                                            agaCTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATgc                    >  2:3044529/1‑150 (MQ=255)
                                                                                                                            agaCTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATgc                    >  1:2046933/1‑150 (MQ=255)
                                                                                                                               cTGTTCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTATTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGcc                 <  1:3538883/150‑1 (MQ=255)
                                                                                                                                  ttCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCAGa              >  2:1429370/1‑150 (MQ=255)
                                                                                                                                   tCGTGTCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCAGAt             <  2:1454881/150‑1 (MQ=255)
                                                                                                                                    cGTGTCTAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCAGATc            >  1:3972032/1‑150 (MQ=255)
                                                                                                                                     gtgtCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCAGATCa           <  1:4196883/150‑1 (MQ=255)
                                                                                                                                     gtgtCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCAGATCa           <  1:2330556/150‑1 (MQ=255)
                                                                                                                                      tgtCGAAGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCAGATCAg          <  2:3989102/150‑1 (MQ=255)
                                                                                                                                        tCGAAGATGGCGATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCAGATCAGcc        >  1:784490/1‑150 (MQ=255)
                                                                                                                                           aaGATGGCTATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCAGATCAGCCGTg     >  2:2815702/1‑150 (MQ=255)
                                                                                                                                              aTGGCTATAAGTGGGCTGAATTTAAGCCGTACAAAGGTTTTTCGCTGGATAACACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCAGATCAGCCGTgcgc  >  2:1350883/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GTTATCGAAGTTAACGCGCCGGAAATCCCGATCATGGACGGCAGCGCCGCTCCGTTTGTATACCTGCTGCTTGACGCCGGTATCGACGAGTTGAACTGCGCCAAAAAATTTGTTCGCATCAAAGAGACTGTTCGTGTCGAAGATGGCGATAAGTGGGCTGAATTTAAGCCGTACAATGGTTTTTCGCTGGATTTCACCATCGATTTTAACCATCCGGCTATTGATTCCAGCAACCAGCGCTATGCGATGAACTTCTCCGCTGATGCGTTTATGCGCCAGATCAGCCGTGCGC  >  CP009273/103320‑103611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑