| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,204,284 | C→A | 50.0% | D98Y (GAT→TAT) | tfaE ← | e14 prophage; putative tail fiber assembly protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,204,284 | 0 | C | A | 50.0% | 8.7 / 43.5 | 32 | D98Y (GAT→TAT) | tfaE | e14 prophage; putative tail fiber assembly protein |
| Reads supporting (aligned to +/- strand): ref base C (7/9); new base A (8/8); total (15/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTGCAGCATCAATCAGTGACTGGCGCTGTGCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTACCAGGGGCATCT > CP009273/1204136‑1204426 | aTTGCAGCATCAATCAGTGACTGGCGCTGTGCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCa > 2:1026461/1‑150 (MQ=255) gcagcaTCAATCAGTGACTGGCGCTGTGCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAAccc > 1:3935908/1‑150 (MQ=255) gcagcaTCAATCAGTGACTGGCGCTGTGCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAAccc > 1:3935912/1‑150 (MQ=255) tgtgCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATtct < 2:912538/150‑1 (MQ=255) gCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCa < 1:682763/150‑1 (MQ=255) gCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCa < 1:3462127/150‑1 (MQ=255) cTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGttt > 2:3609494/1‑150 (MQ=255) tGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAGtttt < 1:141296/150‑1 (MQ=255) ccGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTgg > 2:1709091/1‑150 (MQ=255) ccGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTgg > 2:1709079/1‑150 (MQ=255) ccGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTgg > 2:1708888/1‑150 (MQ=255) gcTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTc < 2:4169134/150‑1 (MQ=255) gcTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTc < 2:1836826/150‑1 (MQ=255) aGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTgg > 1:2307629/1‑150 (MQ=255) tATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGca > 2:878609/1‑150 (MQ=255) tATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGca > 2:1527713/1‑150 (MQ=255) ggTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCGTTGATTTCGTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACata > 2:2463064/1‑150 (MQ=255) ccATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCa > 2:2714946/1‑150 (MQ=255) cATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAAt < 2:2465991/150‑1 (MQ=255) cATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAAt < 2:2465826/150‑1 (MQ=255) aTCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATc > 1:3869370/1‑150 (MQ=255) ccATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGc > 2:2683810/1‑150 (MQ=255) aTTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAg > 2:2502914/1‑150 (MQ=255) aTCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACg < 2:2774186/150‑1 (MQ=255) aTCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACg < 2:2774193/150‑1 (MQ=255) aTCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACg < 2:3590858/150‑1 (MQ=255) gagaTAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTg < 2:2307629/150‑1 (MQ=255) gaTAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATAACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCa < 1:2883884/150‑1 (MQ=255) ttttCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTACCAggg < 1:3666833/150‑1 (MQ=255) ttttCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTACCAggg < 1:3666943/150‑1 (MQ=255) tttCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTACCAgggg < 1:4334784/150‑1 (MQ=255) gggTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTACCAGGGGCATCt < 1:1884075/150‑1 (MQ=255) | ATTGCAGCATCAATCAGTGACTGGCGCTGTGCTTCTGCCGCGTCTACTGCGGCGCTATGCTGTGCTTCAGTATCGGTCACCCATTTCTCACCATCCCATTTATCGTATGGAGATAAAGGGGCGATAGTGGTTGTATTTTCAGGGTAATCACCCGGAGCTTTGATTTCTTTTGATTCTCCAGTTTTGGTGCTATAGACCGTTTCACCGCGATGGTCTGGCACATATTCCCATGAGTTAAAATCTGCAGAACGGCAGATTGCATAACCAGCCTTATGTGTACCAGGGGCATCT > CP009273/1204136‑1204426 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |