Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,226,292 T→A 100% C24S (TGT→AGT)  umuD → DNA polymerase V, subunit D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,226,2920TA96.3% 906.3 / 18.2 267C24S (TGT→AGT) umuDDNA polymerase V, subunit D
Reads supporting (aligned to +/- strand):  ref base T (5/5);  new base A (143/114);  total (148/119)
Fisher's exact test for biased strand distribution p-value = 7.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 271 total aligned reads displayed.

ATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTG  >  CP009273/1226145‑1226438
                                                                                                                                                   |                                                                                                                                                  
aTAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAgagt                                                                                                                                                  <  1:2878920/150‑1 (MQ=255)
  aGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTCTTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTgg                                                                                                                                                >  2:1474768/1‑150 (MQ=255)
  aGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTgg                                                                                                                                                >  2:1133080/1‑150 (MQ=255)
   gCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGc                                                                                                                                               >  2:1887922/1‑150 (MQ=255)
    ccTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCt                                                                                                                                              <  2:420312/150‑1 (MQ=255)
    ccTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCt                                                                                                                                              <  1:2243015/150‑1 (MQ=255)
     cTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCtt                                                                                                                                             >  1:863031/1‑150 (MQ=255)
       gAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTc                                                                                                                                           <  2:3572991/150‑1 (MQ=255)
          tCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCtt                                                                                                                                        <  2:3251216/150‑1 (MQ=255)
            aGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCa                                                                                                                                      >  2:939129/1‑150 (MQ=255)
              tATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCAcc                                                                                                                                    <  1:682886/150‑1 (MQ=255)
                   aTCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGgcag                                                                                                                               >  1:1534314/1‑150 (MQ=255)
                      tgctgGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGcagcag                                                                                                                            >  2:3458814/1‑150 (MQ=255)
                       gctgGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGa                                                                                                                           >  1:964621/1‑150 (MQ=255)
                       gctgGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGa                                                                                                                           >  1:1759971/1‑150 (MQ=255)
                        ctgGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGAt                                                                                                                          >  1:2618559/1‑150 (MQ=255)
                         tgGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGAtt                                                                                                                         <  2:1313832/150‑1 (MQ=255)
                         tgGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGAtt                                                                                                                         >  1:2397541/1‑150 (MQ=255)
                             aaGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGt                                                                                                                     >  1:3950821/1‑150 (MQ=255)
                             aaGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGt                                                                                                                     <  1:3784806/150‑1 (MQ=255)
                              aGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGtt                                                                                                                    >  1:392874/1‑150 (MQ=255)
                                   aGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACa                                                                                                               >  1:1155026/1‑150 (MQ=255)
                                      ctactGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAgcg                                                                                                            <  1:546794/150‑1 (MQ=255)
                                          tGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATc                                                                                                        <  2:102023/150‑1 (MQ=255)
                                           gTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCg                                                                                                       >  2:1535659/1‑150 (MQ=255)
                                            tatataAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGa                                                                                                      <  2:549558/150‑1 (MQ=255)
                                              tataAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATc                                                                                                    >  2:607678/1‑150 (MQ=255)
                                              tataAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATc                                                                                                    <  2:1671749/150‑1 (MQ=255)
                                              tataAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATc                                                                                                    <  2:2800083/150‑1 (MQ=255)
                                               ataAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCt                                                                                                   >  1:3736407/1‑150 (MQ=255)
                                               ataAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCt                                                                                                   >  2:462694/1‑150 (MQ=255)
                                               ataAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCt                                                                                                   <  1:4212880/150‑1 (MQ=255)
                                                  aaaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGaa                                                                                                >  1:3009322/1‑150 (MQ=255)
                                                  aaaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGaa                                                                                                >  2:881207/1‑150 (MQ=255)
                                                  aaaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGaa                                                                                                >  1:3420155/1‑150 (MQ=255)
                                                  aaaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGaa                                                                                                <  1:2594750/150‑1 (MQ=255)
                                                    aaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTATGTTGAACAGCGCATCGATCTGAATc                                                                                              >  2:2294660/1‑150 (MQ=255)
                                                      cAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCaa                                                                                            <  2:2056566/150‑1 (MQ=255)
                                                          ataACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGt                                                                                        >  1:2190240/1‑150 (MQ=255)
                                                          ataACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGt                                                                                        >  1:1637524/1‑150 (MQ=255)
                                                           taACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGtt                                                                                       >  1:2458880/1‑150 (MQ=255)
                                                             aCTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGa                                                                                     >  1:907720/1‑150 (MQ=255)
                                                                 cAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCa                                                                                 <  2:181483/150‑1 (MQ=255)
                                                                    gCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCa                                                                              <  2:2217817/150‑1 (MQ=255)
                                                                     cAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCAt                                                                             >  2:3809900/1‑150 (MQ=255)
                                                                      aGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATc                                                                            <  1:2275817/150‑1 (MQ=255)
                                                                       gATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATcc                                                                           >  2:4061526/1‑150 (MQ=255)
                                                                         ttattaTGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCa                                                                         <  2:137745/150‑1 (MQ=255)
                                                                         ttattaTGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCa                                                                         <  2:4582748/150‑1 (MQ=255)
                                                                           attatGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAgc                                                                       >  2:3085263/1‑150 (MQ=255)
                                                                           attatGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAgc                                                                       <  2:2966626/150‑1 (MQ=255)
                                                                             tatGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTAGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAgcgc                                                                     >  2:657883/1‑150 (MQ=255)
                                                                              attttgttTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGcgcg                                                                    >  2:4462549/4‑150 (MQ=255)
                                                                              atGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGcgcg                                                                    <  1:744390/150‑1 (MQ=255)
                                                                                gttgttTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGac                                                                  >  2:2357971/1‑150 (MQ=255)
                                                                                 ttgttTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGact                                                                 <  1:100662/150‑1 (MQ=255)
                                                                                 ttgttTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGact                                                                 >  1:3322761/1‑150 (MQ=255)
                                                                                  tgttTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGactt                                                                >  1:1500140/1‑150 (MQ=255)
                                                                                  tgttTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGactt                                                                >  1:4630822/1‑150 (MQ=255)
                                                                                     ttATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGacttact                                                             <  2:4187773/150‑1 (MQ=255)
                                                                                     ttATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGacttact                                                             >  2:2733478/1‑150 (MQ=255)
                                                                                       aTCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTc                                                           <  2:118399/150‑1 (MQ=255)
                                                                                            gCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCaa                                                      >  1:3647683/1‑150 (MQ=255)
                                                                                             ccTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCaaa                                                     <  1:1024520/150‑1 (MQ=255)
                                                                                             ccTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCaaa                                                     >  2:1843875/1‑150 (MQ=255)
                                                                                              cTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAg                                                    <  1:2794654/150‑1 (MQ=255)
                                                                                              cTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAg                                                    <  1:476622/150‑1 (MQ=255)
                                                                                               tGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGc                                                   >  1:3299532/1‑150 (MQ=255)
                                                                                                 cGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCaa                                                 >  1:3987840/1‑150 (MQ=255)
                                                                                                   gATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgt                                               >  2:538693/1‑150 (MQ=255)
                                                                                                   gATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgt                                               <  1:1272113/150‑1 (MQ=255)
                                                                                                    aTCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtg                                              >  1:2013381/1‑150 (MQ=255)
                                                                                                    aTCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtg                                              <  1:1594707/150‑1 (MQ=255)
                                                                                                    aTCTCCGCGAAATTGTGACTTTTCCGCTAAATAGCGATCTTGTTCAGAGTGGCTTACCATCACCGGCAGCAGATAACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtg                                              >  1:4256223/1‑150 (MQ=255)
                                                                                                      ctcCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtggt                                            <  1:1572783/150‑1 (MQ=255)
                                                                                                           cgAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTc                                       <  1:213974/150‑1 (MQ=255)
                                                                                                            gAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCt                                      >  2:3814825/1‑150 (MQ=255)
                                                                                                             aaaTTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTa                                     <  2:786438/150‑1 (MQ=255)
                                                                                                               aTTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAtg                                   >  1:3025618/1‑150 (MQ=255)
                                                                                                               aTTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAtg                                   <  2:1633425/150‑1 (MQ=255)
                                                                                                                    gACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAtgattga                              >  2:4145955/1‑150 (MQ=255)
                                                                                                                    gACTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACATTTGATCCAGCATCCCAGCGCGACTTACATCGTCAAATCAACTGGTGATTCGAtgattga                              >  2:388660/1‑150 (MQ=255)
                                                                                                                      cTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtg                            >  2:458896/1‑150 (MQ=255)
                                                                                                                      cTTTTCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtg                            <  1:4379110/150‑1 (MQ=255)
                                                                                                                        tttCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtggt                          >  1:711422/1‑150 (MQ=255)
                                                                                                                        tttCCGCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtggt                          >  2:2883162/1‑150 (MQ=255)
                                                                                                                             gCTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAAt                     >  2:2605482/1‑150 (MQ=255)
                                                                                                                              cTATTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAAtt                    <  2:2946912/150‑1 (MQ=255)
                                                                                                                                aTTTAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAg                  >  2:2287433/1‑150 (MQ=255)
                                                                                                                                  ttAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTg                <  2:2611488/150‑1 (MQ=255)
                                                                                                                                  ttAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTg                >  1:2671004/1‑150 (MQ=255)
                                                                                                                                  ttAGCGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTg                >  2:1481349/1‑150 (MQ=255)
                                                                                                                                      cGATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACgg            >  2:2570841/1‑150 (MQ=255)
                                                                                                                                       gATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGt           <  1:4583764/150‑1 (MQ=255)
                                                                                                                                       gATCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGt           >  1:1803112/1‑150 (MQ=255)
                                                                                                                                         tCTTGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGa         <  2:2444868/150‑1 (MQ=255)
                                                                                                                                           ttGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGAtt       >  1:3144646/1‑150 (MQ=255)
                                                                                                                                           ttGTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGAAGGTGAtt       >  1:1764968/1‑150 (MQ=255)
                                                                                                                                             gTTCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTa     <  2:3912471/150‑1 (MQ=255)
                                                                                                                                               tCAGAGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACt   <  1:3376795/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTG  >  CP009273/1226145‑1226438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑