BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,240,865	A→T	9.1%	K84* (AAA→TAA)	ycgY →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,240,865	0	A	T	9.1%	732.9 / 48.4	229	K84* (AAA→TAA)	ycgY	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base A (95/113); new base T (7/14); total (102/127)
Fisher's exact test for biased strand distribution p-value = 3.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 247 total aligned reads displayed.

TGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTT  >  CP009273/1240716‑1241014

tGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCaaaa                                                                                                                                                       >  1:2470097/1‑150 (MQ=255)

  aTCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCaaaaaa                                                                                                                                                     <  1:3873874/150‑1 (MQ=255)

  aTCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCaaaaaa                                                                                                                                                     >  1:754581/1‑150 (MQ=255)

    cAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAAc                                                                                                                                                   <  1:3094052/150‑1 (MQ=255)

    cAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAAc                                                                                                                                                   <  1:1303079/150‑1 (MQ=255)

       tCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGtt                                                                                                                                                <  2:2119007/150‑1 (MQ=255)

            cTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTtgatga                                                                                                                                           <  2:4318404/150‑1 (MQ=255)

                  gATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAac                                                                                                                                     <  1:1752282/150‑1 (MQ=255)

                  gATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAac                                                                                                                                     >  1:3007945/1‑150 (MQ=255)

                  gATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAac                                                                                                                                     <  1:2213143/150‑1 (MQ=255)

                   aTTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAaca                                                                                                                                    <  2:833483/150‑1 (MQ=255)

                     tAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACAcc                                                                                                                                  >  2:3595380/1‑150 (MQ=255)

                      aaGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCg                                                                                                                                 <  1:2043142/150‑1 (MQ=255)

                        gCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTa                                                                                                                               <  1:3167293/150‑1 (MQ=255)

                           aaaaTCCCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACa                                                                                                                            <  2:746902/150‑1 (MQ=255)

                              aTACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAata                                                                                                                         <  2:3260682/150‑1 (MQ=255)

                              aTACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAata                                                                                                                         <  1:1007252/150‑1 (MQ=255)

                                aCCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATAtt                                                                                                                       >  1:3738923/1‑150 (MQ=255)

                                  ccGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCa                                                                                                                     <  1:1204153/150‑1 (MQ=255)

                                    gTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAgg                                                                                                                   <  1:4163021/150‑1 (MQ=255)

                                      tCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTa                                                                                                                 >  2:2178119/1‑150 (MQ=255)

                                          cAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCaa                                                                                                             <  1:1063795/150‑1 (MQ=255)

                                          cAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCaa                                                                                                             >  1:3133480/1‑150 (MQ=255)

                                           aaTGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAAt                                                                                                            <  2:2014732/150‑1 (MQ=255)

                                           aaTGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAAt                                                                                                            <  1:63347/150‑1 (MQ=255)

                                            aTGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAAtt                                                                                                           <  2:3640674/150‑1 (MQ=255)

                                             tGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTg                                                                                                          <  2:4056568/150‑1 (MQ=255)

                                              gCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAATAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGa                                                                                                         >  1:152785/1‑150 (MQ=255)

                                              gCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGa                                                                                                         <  2:4204619/150‑1 (MQ=255)

                                               cTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGAt                                                                                                        <  2:2754215/150‑1 (MQ=255)

                                                ttCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATg                                                                                                       >  2:154773/1‑150 (MQ=255)

                                                 tCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGc                                                                                                      >  2:246197/1‑150 (MQ=255)

                                                 tCAGTTACGAGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAAAACCGTAACAATATTCAGGTAGCAATTGATGc                                                                                                      >  2:3277412/1‑150 (MQ=255)

                                                  cAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCt                                                                                                     <  2:1106536/150‑1 (MQ=255)

                                                  cAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCt                                                                                                     <  1:3259195/150‑1 (MQ=255)

                                                   aGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCtt                                                                                                    >  1:2733796/1‑150 (MQ=255)

                                                    gTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCttt                                                                                                   >  1:1125224/1‑150 (MQ=255)

                                                    gTTACGCGAAGAGGAATGGTCTGAATTCATTTTTTTGTTGCTAAAATCTCTGGAGTGTCTGGATTATGTGATTATAAATTTAACTCCAGAAAGCAAATAAACGTTGATGAGTGAACACCGTAACAATATTCAGTTAGCAATTGATGCttt                                                                                                   >  1:4568392/1‑150 (MQ=255)

                                                        cgcgAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTtatat                                                                                               <  2:1597956/150‑1 (MQ=255)

                                                        cgcgAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTtatat                                                                                               >  2:2384840/1‑150 (MQ=255)

                                                          cgAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAATAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAg                                                                                             <  1:3914328/150‑1 (MQ=255)

                                                          cgAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAg                                                                                             >  1:943336/1‑150 (MQ=255)

                                                           gAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGc                                                                                            <  1:3385516/150‑1 (MQ=255)

                                                            aaGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGcc                                                                                           >  2:491731/1‑150 (MQ=255)

                                                             agagGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAATAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCa                                                                                          <  2:2573007/150‑1 (MQ=255)

                                                               agGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAAc                                                                                        >  1:3436397/1‑150 (MQ=255)

                                                                  aaTGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTc                                                                                     <  2:3920065/150‑1 (MQ=255)

                                                                   aTGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCg                                                                                    <  1:513633/150‑1 (MQ=255)

                                                                    tGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAATAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCgg                                                                                   <  2:4174213/150‑1 (MQ=255)

                                                                    tGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCgg                                                                                   >  2:1727906/1‑150 (MQ=255)

                                                                     ggTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGc                                                                                  >  2:4503565/1‑150 (MQ=255)

                                                                     ggTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGc                                                                                  <  2:2214826/150‑1 (MQ=255)

                                                                        cTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTa                                                                               >  2:1641624/1‑150 (MQ=255)

                                                                          gAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTaaa                                                                             >  2:1885940/1‑150 (MQ=255)

                                                                          gAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTaaa                                                                             >  1:1895926/1‑150 (MQ=255)

                                                                           aaTTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAAt                                                                            >  1:2928874/1‑150 (MQ=255)

                                                                                    tttGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGtg                                                                   <  2:3084458/150‑1 (MQ=255)

                                                                                       ggTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGtgatg                                                                <  2:2666019/150‑1 (MQ=255)

                                                                                                   ctctGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAacac                                                    >  2:4230875/1‑150 (MQ=255)

                                                                                                     ctGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACtt                                                  <  1:1380659/150‑1 (MQ=255)

                                                                                                      tGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTa                                                 <  1:1665511/150‑1 (MQ=255)

                                                                                                       ggAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTAc                                                >  1:2814743/1‑150 (MQ=255)

                                                                                                          gtgtCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCg                                             <  1:1501198/150‑1 (MQ=255)

                                                                                                             tCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAg                                          <  1:2090607/150‑1 (MQ=255)

                                                                                                             tCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGACGGGTGATGAAAGTGAAACACTTACCCGTAg                                          >  2:3673943/1‑150 (MQ=255)

                                                                                                              cTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAATAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTGCCCGTAGa                                         >  2:608705/1‑150 (MQ=255)

                                                                                                               tGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGaa                                        >  2:2157622/1‑150 (MQ=255)

                                                                                                               tGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGaa                                        <  1:2281086/150‑1 (MQ=255)

                                                                                                               tGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGaa                                        >  2:3858525/1‑150 (MQ=255)

                                                                                                               tGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGaa                                        >  1:1792321/1‑150 (MQ=255)

                                                                                                                ggATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGaaa                                       <  1:291495/150‑1 (MQ=255)

                                                                                                                ggATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGaaa                                       >  2:3766180/1‑150 (MQ=255)

                                                                                                                ggATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGaaa                                       >  2:1455750/1‑150 (MQ=255)

                                                                                                                 gATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAAt                                      >  1:2559055/1‑150 (MQ=255)

                                                                                                                  aTTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATg                                     <  1:4495835/150‑1 (MQ=255)

                                                                                                                    tATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGAc                                   >  1:4123589/1‑150 (MQ=255)

                                                                                                                    tATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGAc                                   <  1:1698004/150‑1 (MQ=255)

                                                                                                                     aTGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACg                                  >  2:3906711/1‑150 (MQ=255)

                                                                                                                         gATTATTAATTTAACTCCAGAAAGCAAATAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTAt                              >  1:950155/1‑150 (MQ=255)

                                                                                                                         gATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTAt                              <  2:3007945/150‑1 (MQ=255)

                                                                                                                             attaATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTc                          >  2:1239676/1‑150 (MQ=255)

                                                                                                                              ttaATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCg                         <  2:4452712/150‑1 (MQ=255)

                                                                                                                              ttaATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCg                         <  2:1763802/150‑1 (MQ=255)

                                                                                                                                aaTTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGc                       >  1:362577/1‑150 (MQ=255)

                                                                                                                                  tttAACTCCAGAAAGCAAATAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCaa                     <  2:4568392/150‑1 (MQ=255)

                                                                                                                                   ttAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAAc                    <  2:3394462/150‑1 (MQ=255)

                                                                                                                                      aCTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCAc                 <  2:1851088/150‑1 (MQ=255)

                                                                                                                                       cTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACg                <  2:1017272/150‑1 (MQ=255)

                                                                                                                                        tCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGt               <  2:256500/150‑1 (MQ=255)

                                                                                                                                        tCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGt               <  1:2282858/150‑1 (MQ=255)

                                                                                                                                              aaGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCa         >  1:2881994/1‑150 (MQ=255)

                                                                                                                                              aaGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCa         >  1:2103724/1‑150 (MQ=255)

                                                                                                                                              aaGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCa         >  1:2729673/1‑150 (MQ=255)

                                                                                                                                              aaGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGAAGAAATGACGCTATCATCGGCAACCACGTATGGCa         >  2:1563126/1‑150 (MQ=255)

                                                                                                                                               aGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCaa        <  2:3738924/150‑1 (MQ=255)

                                                                                                                                                 cAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAAc      >  2:1210250/1‑150 (MQ=255)

                                                                                                                                                  aaaaaaaCGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACa     <  1:4218426/150‑1 (MQ=255)

                                                                                                                                                   aaaaaaCGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACAt    >  1:1083650/1‑150 (MQ=255)

                                                                                                                                                     aaaaCGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACAttt  >  1:3039493/1‑150 (MQ=255)

                                                                                                                                                     aaaaCGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACAttt  <  1:743383/150‑1 (MQ=255)

TGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTT  >  CP009273/1240716‑1241014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`