Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 107,298 C→A 36.3% R845S (CGT→AGT)  secA → preprotein translocase subunit, ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273107,2980CA36.3% 345.5 / 220.9 245R845S (CGT→AGT) secApreprotein translocase subunit, ATPase
Reads supporting (aligned to +/- strand):  ref base C (81/74);  new base A (57/33);  total (138/107)
Fisher's exact test for biased strand distribution p-value = 1.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 251 total aligned reads displayed.

ACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTAACGATCCTTGCCCGTGCGGTTCTGGTAAAAAATACAAG  >  CP009273/107150‑107447
                                                                                                                                                    |                                                                                                                                                     
  aGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTa                                                                                                                                                    >  2:3048327/1‑150 (MQ=255)
   gAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAATTTATCAGTACGCTGAGCAAAGTTCAGGTACGGATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTAt                                                                                                                                                   >  1:1392824/1‑150 (MQ=255)
    aaaGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATg                                                                                                                                                  <  1:3137685/150‑1 (MQ=255)
    aaaGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTAGTATg                                                                                                                                                  <  1:3840127/150‑1 (MQ=255)
    aaaGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGAAATATGAAGTTATAAGTACGCTGAGCAAAGTTAAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATg                                                                                                                                                  >  1:3499002/1‑150 (MQ=255)
      aGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGGTATCAGTACGCGGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTAGTATGGa                                                                                                                                                >  2:4220097/1‑150 (MQ=255)
        aTCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAg                                                                                                                                              >  1:1030424/1‑150 (MQ=255)
        aTCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAg                                                                                                                                              <  1:3524262/150‑1 (MQ=255)
         tCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGc                                                                                                                                             >  1:1152660/1‑150 (MQ=255)
         tCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTAGTATGGAAGc                                                                                                                                             >  2:2273796/1‑150 (MQ=255)
            gAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGa                                                                                                                                          <  2:3860216/150‑1 (MQ=255)
             aaGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGTAAAAAAGAAGTTATAACTTAACAGATCAAAATTAAAGTAAGTATTCCAAAATAAGTTGAGGATGGGGAAAAAAAACGTCGTATGGAACCCGAg                                                                                                                                         >  1:1203810/1‑150 (MQ=255)
              aGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGc                                                                                                                                        <  2:675467/150‑1 (MQ=255)
              aGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGc                                                                                                                                        <  2:674913/150‑1 (MQ=255)
              aGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGc                                                                                                                                        <  2:2987743/150‑1 (MQ=255)
                  ggAATACAATCGTGTATCTTTCTCCTTGTTTGCAGCGATGCTGGAGTCGTTGATATATGTAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCTTATGGAAGCCGAGCGttt                                                                                                                                    <  2:4220400/150‑1 (MQ=255)
                  ggAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTAGTATGGAAGCCGAGCGttt                                                                                                                                    <  1:2590221/150‑1 (MQ=255)
                   gAATACAAACGTGTATCGTTCTCCTTGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCTGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTa                                                                                                                                   <  1:4215189/150‑1 (MQ=255)
                    aaTACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAg                                                                                                                                  <  1:2848295/150‑1 (MQ=255)
                    aaTACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCGGGAACAACAGCGTAGTATGGAAGCCGAGCGTTTAg                                                                                                                                  >  1:3847822/1‑150 (MQ=255)
                     aTACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAgc                                                                                                                                 >  2:3108655/1‑150 (MQ=255)
                      tACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCCGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAgcg                                                                                                                                <  1:3753538/150‑1 (MQ=255)
                       aCAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGGAATATTAAGTTATAAATACGCGGAGCAAAGTTAAGGTACGTATTCCAGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATTGAAGCCGAGCGTTTAgcgc                                                                                                                               >  1:1596575/1‑150 (MQ=255)
                       aCAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAgcgc                                                                                                                               >  2:4488669/1‑150 (MQ=255)
                        cAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCa                                                                                                                              <  1:3264479/150‑1 (MQ=255)
                              tGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGGAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATgca                                                                                                                        >  2:1561931/1‑150 (MQ=255)
                              tGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATgca                                                                                                                        >  2:2396262/1‑150 (MQ=255)
                                aaTCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAATAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATgcagc                                                                                                                      >  2:3796416/1‑150 (MQ=255)
                                    gTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAATTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCt                                                                                                                  >  1:1100420/1‑150 (MQ=255)
                                    gTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCt                                                                                                                  <  2:3200533/150‑1 (MQ=255)
                                     ttCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCtt                                                                                                                 <  1:51211/150‑1 (MQ=255)
                                     ttCTCCATGTTTGCAGCGATGCTGGAGTCGTGGTAAAATGAAGTTATCAGTAAGCGGAGCAAAGTTAAGGTACGTATGCCGGAAGCGGGTGAGGAGCTGGAACAACAGCGTAGTATGGAAGCCGAGCGTTTATCGCAAAAGACGCAGCtt                                                                                                                 >  1:2245523/1‑150 (MQ=255)
                                       ctcCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTAGTATGGAAGCCGAGCGTTTAGCGCAAAAGCAGCAGCGTAg                                                                                                               >  2:1320266/1‑150 (MQ=255)
                                         ccATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCCAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTAGTATTGAAGCCGAGCGTTTAGCGCAAATTCAGCAGCTTAGcc                                                                                                             >  1:4080229/1‑150 (MQ=255)
                                         ccATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGcc                                                                                                             <  2:3878989/150‑1 (MQ=255)
                                         ccATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAACGCAGCAGCTTAGcc                                                                                                             <  2:4621449/150‑1 (MQ=255)
                                         ccATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATAAGTACGCTGAGCAAAGTTAAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTAGTATGGAAGCCGAGCGTTTAGCGCAAAAGCAGCAGCGTAGcc                                                                                                             >  1:1652724/1‑150 (MQ=255)
                                           aTGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTAGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCAt                                                                                                           <  2:3245472/150‑1 (MQ=255)
                                             gTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCa                                                                                                         <  2:4315481/150‑1 (MQ=255)
                                              tttGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAg                                                                                                        <  1:651154/150‑1 (MQ=255)
                                               ttGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGTGGAGCTGGAACAACAGCGTAGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAgg                                                                                                       >  2:2534257/1‑150 (MQ=255)
                                                tGCAGCGATGCTGGAGTCGTTGTAATATGAAGTTATCAGGAAGCAGAGCAAAGTTCAGGTACGTATGCCTGTAGAGGTTGAGGAGCTGGAAAAACAGCGGCGTATGGAAGCCCAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGa                                                                                                      >  1:891839/1‑150 (MQ=255)
                                                  cAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATg                                                                                                    >  2:2778794/1‑150 (MQ=255)
                                                  cAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATg                                                                                                    >  2:305308/1‑150 (MQ=255)
                                                  cAGCGATGCTGGAGTCGTTGAAATATGAAGTTATAAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGGATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCAACAGGATg                                                                                                    >  1:1820955/1‑150 (MQ=255)
                                                   aGCGATGCTGGAGTCGTGGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGGTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATga                                                                                                   >  2:209342/1‑150 (MQ=255)
                                                        tGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGAct                                                                                              <  2:1592163/150‑1 (MQ=255)
                                                         gCTGGAGTCGTTGAAAAATGAAGTTATCAGTAAGCAGAGCAAATTTCAGGTAAGTATGCCAGAATAGGTTGATGAGCTGGAACAACACCGTAGTATGGAAGCCCAGCGTTTAGCGCAAATGCAGCAGCTTAGCCAACAGGATTACGAatc                                                                                             >  1:1852432/1‑150 (MQ=255)
                                                          cTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTAGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGActct                                                                                            <  1:3461444/150‑1 (MQ=255)
                                                              tgTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAg                                                                                        <  2:1203810/149‑1 (MQ=255)
                                                                tCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTAGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGcc                                                                                      <  2:1464034/150‑1 (MQ=255)
                                                                    tGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAg                                                                                  >  1:4639731/1‑150 (MQ=255)
                                                                    tGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAg                                                                                  <  1:2277394/150‑1 (MQ=255)
                                                                     gAAATATGAAGTTATCTGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGc                                                                                 <  1:1572102/150‑1 (MQ=255)
                                                                         tatGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTAGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCa                                                                             >  1:12481/1‑150 (MQ=255)
                                                                           tGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACt                                                                           <  1:4558986/150‑1 (MQ=255)
                                                                           tGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACt                                                                           <  2:1726529/150‑1 (MQ=255)
                                                                            gAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTg                                                                          >  2:3511840/1‑150 (MQ=255)
                                                                             aaGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTgg                                                                         <  2:2597273/150‑1 (MQ=255)
                                                                              aGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTgga                                                                        >  2:4170419/1‑149 (MQ=255)
                                                                              aGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTAGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTggc                                                                        <  2:2716263/150‑1 (MQ=255)
                                                                                   tCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGgcgc                                                                   <  1:3607255/150‑1 (MQ=255)
                                                                                    cAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCa                                                                  >  2:3597542/1‑150 (MQ=255)
                                                                                      gTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTAGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCaaa                                                                >  1:2264724/1‑150 (MQ=255)
                                                                                       tACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAAc                                                               >  1:2478637/1‑150 (MQ=255)
                                                                                         cGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTAGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCg                                                             <  1:235188/150‑1 (MQ=255)
                                                                                          gCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCgg                                                            >  1:1284839/1‑150 (MQ=255)
                                                                                          gCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTAGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCgg                                                            <  1:255538/150‑1 (MQ=255)
                                                                                            tGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTAGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGgag                                                          >  1:3712015/1‑150 (MQ=255)
                                                                                             gAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTAGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGgaga                                                         >  1:149769/1‑150 (MQ=255)
                                                                                                cAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAgcg                                                      <  2:3912602/150‑1 (MQ=255)
                                                                                                cAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAgcg                                                      <  2:4037449/150‑1 (MQ=255)
                                                                                                 aaaGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTAGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAgcgc                                                     >  1:996355/1‑150 (MQ=255)
                                                                                                   aGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTAGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCaa                                                   <  2:1706748/150‑1 (MQ=255)
                                                                                                    gTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTAGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCaaa                                                  >  1:4243583/1‑150 (MQ=255)
                                                                                                      tCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTAGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGt                                                >  2:373550/1‑150 (MQ=255)
                                                                                                      tCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTAGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGt                                                >  1:1436214/1‑150 (MQ=255)
                                                                                                       cAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTa                                               >  1:1786497/1‑150 (MQ=255)
                                                                                                         ggTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTAGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAgg                                             <  1:2007273/150‑1 (MQ=255)
                                                                                                          gTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGa                                            >  2:3298744/1‑150 (MQ=255)
                                                                                                           tACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGAc                                           >  1:4433231/1‑150 (MQ=255)
                                                                                                           tACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGAc                                           >  1:2661999/1‑150 (MQ=255)
                                                                                                              gTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTa                                        >  1:3088346/1‑150 (MQ=255)
                                                                                                              gTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTa                                        >  1:3088318/1‑150 (MQ=255)
                                                                                                                    cTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTAACGATc                                  <  1:641276/150‑1 (MQ=255)
                                                                                                                      gAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTAACGATCCt                                >  1:825392/1‑150 (MQ=255)
                                                                                                                          agGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTAACGATCCTTGcc                            <  1:2852662/150‑1 (MQ=255)
                                                                                                                          agGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTAACGATCCTTGcc                            >  2:889543/1‑150 (MQ=255)
                                                                                                                            gTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTAACGATCCTTGCCCg                          <  2:1702104/150‑1 (MQ=255)
                                                                                                                             ttGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTAACGATCCTTGCCCGt                         >  2:2464265/1‑150 (MQ=255)
                                                                                                                              tGAGGAGCTGGAACAACAGCGTAGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTAACGATCCTTGCCCGTg                        >  2:2842500/1‑150 (MQ=255)
                                                                                                                                aggagCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTAACGATCCTTGCCCGTGCg                      <  1:1695630/150‑1 (MQ=255)
                                                                                                                                  gagCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTAACGATCCTTGCCCGTGCGGt                    >  2:1827044/1‑150 (MQ=255)
                                                                                                                                   agCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTAACGATCCTTGCCCGTGCGGtt                   <  1:390887/150‑1 (MQ=255)
                                                                                                                                   agCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGACATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTAACGATCCTTGCCCGTGCGGtt                   >  1:1539878/1‑150 (MQ=255)
                                                                                                                                     cTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTAACGATCCTTGCCCGTGCGGTTCt                 <  1:2743319/150‑1 (MQ=255)
                                                                                                                                        gAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTAACGATCCTTGCCCGTGCGGTTCTGGt              <  2:1392824/150‑1 (MQ=255)
                                                                                                                                          acaacaGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTAACGATCCTTGCCCGTGCGGTTCTGGTaa            >  1:1956407/1‑150 (MQ=255)
                                                                                                                                              caGCGTAGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTAACGATCCTTGCCCGTGCGGTTCTGGTaaaaaa        <  1:699931/150‑1 (MQ=255)
                                                                                                                                                 cgtagtATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAACCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTAACGATCCTTGCCCGTGCGGTTCTGGTAAAAAATAc     >  2:1025036/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAGCTGCACTGGCGGCGCAAACCGGAGAGCGCAAAGTAGGACGTAACGATCCTTGCCCGTGCGGTTCTGGTAAAAAATACAAG  >  CP009273/107150‑107447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑