Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,253,739 C→A 5.3% A77A (GCG→GCT)  pth ← peptidyl‑tRNA hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,253,7390CA 5.3% 1092.2 / 19.3 281A77A (GCG→GCT) pthpeptidyl‑tRNA hydrolase
Reads supporting (aligned to +/- strand):  ref base C (155/110);  new base A (9/7);  total (164/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 299 total aligned reads displayed.

TTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAAC  >  CP009273/1253590‑1253888
                                                                                                                                                     |                                                                                                                                                     
ttattaCCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATc                                                                                                                                                       >  2:4534282/1‑150 (MQ=255)
  attaCCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATcgc                                                                                                                                                     >  2:646007/1‑150 (MQ=255)
   ttaCCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATcgcc                                                                                                                                                    <  1:2698047/150‑1 (MQ=255)
   ttaCCCAATTTACTGAAGATGTCTTTCAGTCCATTGTGACCTCCATGGCCATCGCCTTTTTTATTTTTGGTGACGCCAGGAGGCAGATCCTGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGATAAAACTGGCCATcgcc                                                                                                                                                    <  2:4242727/150‑1 (MQ=255)
    taCCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATcgccg                                                                                                                                                   >  2:2731098/1‑150 (MQ=255)
     aCCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATcgccgc                                                                                                                                                  >  2:796431/1‑150 (MQ=255)
     aCCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATcgccgc                                                                                                                                                  >  2:1458483/1‑150 (MQ=255)
        cAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAAc                                                                                                                                               <  2:3714750/150‑1 (MQ=255)
        cAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAAc                                                                                                                                               <  2:2798934/150‑1 (MQ=255)
         aaTTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACg                                                                                                                                              >  2:3059534/1‑150 (MQ=255)
           tttACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGc                                                                                                                                            >  1:119577/1‑150 (MQ=255)
            ttACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCt                                                                                                                                           >  2:628166/1‑150 (MQ=255)
              aCTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCttt                                                                                                                                         <  2:895260/150‑1 (MQ=255)
              aCTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCttt                                                                                                                                         >  2:1143141/1‑150 (MQ=255)
               cTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTg                                                                                                                                        >  1:594341/1‑150 (MQ=255)
                tgatgaTGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGc                                                                                                                                       <  1:3776959/150‑1 (MQ=255)
                  atgatgTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATAGCCGCAACGGCTTTGCCg                                                                                                                                     <  2:3308391/150‑1 (MQ=255)
                    gatgTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCt                                                                                                                                   <  2:2713858/150‑1 (MQ=255)
                    gatgTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCt                                                                                                                                   >  1:3968060/1‑150 (MQ=255)
                     atgTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTg                                                                                                                                  >  2:2682741/1‑150 (MQ=255)
                       gTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTgag                                                                                                                                >  2:4444013/1‑150 (MQ=255)
                         cTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGAt                                                                                                                              <  2:1581376/150‑1 (MQ=255)
                            tCAGTCCATTGTGACCACCATGGCCATCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCa                                                                                                                           <  1:3397356/150‑1 (MQ=255)
                             cAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCAt                                                                                                                          >  2:4105050/1‑150 (MQ=255)
                                  cATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGTAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATg                                                                                                                     >  2:1845628/1‑150 (MQ=255)
                                   aTTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGt                                                                                                                    >  2:2230946/1‑150 (MQ=255)
                                   aTTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGt                                                                                                                    <  1:470915/150‑1 (MQ=255)
                                     tgtgACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGGTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAg                                                                                                                  >  2:2757364/1‑150 (MQ=255)
                                       tgACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTc                                                                                                                >  2:2178264/1‑150 (MQ=255)
                                         accaccATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCgg                                                                                                              >  1:4642303/1‑150 (MQ=255)
                                          ccaccaTGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCggg                                                                                                             <  1:4563522/150‑1 (MQ=255)
                                          ccaccaTGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCggg                                                                                                             <  1:902832/150‑1 (MQ=255)
                                             ccaTGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTATTGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACt                                                                                                          <  1:3005482/150‑1 (MQ=255)
                                                   ccACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCGAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAgg                                                                                                    >  1:1159717/1‑150 (MQ=255)
                                                   ccACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAgg                                                                                                    >  1:2884516/1‑150 (MQ=255)
                                                            aaTTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCt                                                                                           <  2:2561644/150‑1 (MQ=255)
                                                             aTTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCtt                                                                                          >  2:711223/1‑150 (MQ=255)
                                                              tttAACTTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTc                                                                                         <  2:4322830/150‑1 (MQ=255)
                                                              tttAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTc                                                                                         >  1:507181/1‑150 (MQ=255)
                                                                  aaTTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCt                                                                                     >  1:1890470/1‑150 (MQ=255)
                                                                   aTTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTc                                                                                    <  2:2348219/150‑1 (MQ=255)
                                                                   aTTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGAGTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTc                                                                                    >  2:1220268/1‑150 (MQ=255)
                                                                   aTTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCAGCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTc                                                                                    <  1:1294366/150‑1 (MQ=255)
                                                                      tGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCaa                                                                                 <  2:2394912/150‑1 (MQ=255)
                                                                       ggCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAag                                                                                >  2:2039709/1‑150 (MQ=255)
                                                                       ggCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTCGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAag                                                                                <  1:4410849/150‑1 (MQ=255)
                                                                        gCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATAGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAaga                                                                               <  2:2800805/150‑1 (MQ=255)
                                                                         cGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAagag                                                                              <  1:3912055/150‑1 (MQ=255)
                                                                              ccAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATAGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACt                                                                         <  1:3136993/150‑1 (MQ=255)
                                                                               cAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTc                                                                        <  2:2217934/150‑1 (MQ=255)
                                                                                aggaggCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTcg                                                                       >  1:4496858/1‑150 (MQ=255)
                                                                                 ggaggCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTcgc                                                                      >  1:2295604/1‑150 (MQ=255)
                                                                                 ggaggCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTcgc                                                                      >  1:2508467/1‑150 (MQ=255)
                                                                                  gaggCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTcgcg                                                                     <  1:3851941/150‑1 (MQ=255)
                                                                                       aGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGta                                                                >  2:1007628/1‑150 (MQ=255)
                                                                                        gATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGtat                                                               <  1:222074/150‑1 (MQ=255)
                                                                                         aTCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGtata                                                              <  1:3294958/150‑1 (MQ=255)
                                                                                           ccAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAAc                                                            <  2:1290420/150‑1 (MQ=255)
                                                                                            cAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAAcc                                                           <  2:1636855/150‑1 (MQ=255)
                                                                                            cAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAAcc                                                           >  1:141359/1‑150 (MQ=255)
                                                                                            cAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATAGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAAcc                                                           >  2:1895967/1‑150 (MQ=255)
                                                                                             aGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCa                                                          >  2:2265292/1‑150 (MQ=255)
                                                                                             aGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCa                                                          >  2:195581/1‑150 (MQ=255)
                                                                                              gTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCaa                                                         >  2:4499045/1‑150 (MQ=255)
                                                                                              gTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCaa                                                         >  2:1434669/1‑150 (MQ=255)
                                                                                              gTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATAGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCaa                                                         >  2:3332581/1‑150 (MQ=255)
                                                                                               ttCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCaaa                                                        >  1:2600786/1‑150 (MQ=255)
                                                                                                 cgtcgtGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGa                                                      >  2:4637272/1‑150 (MQ=255)
                                                                                                 cgtcgtGGGCCACCAGAAGTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGa                                                      <  2:1739818/150‑1 (MQ=255)
                                                                                                    cgtGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAAtt                                                   <  2:1027917/150‑1 (MQ=255)
                                                                                                     gtGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAAttt                                                  <  1:2964138/150‑1 (MQ=255)
                                                                                                       gggCCACCAGAATTTCGTCCGGATTAATGCGGAAAAATCTGGCCATAGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGATTGACTCGCGAAGTATAACCAAAGAATTTAg                                                <  1:103231/150‑1 (MQ=255)
                                                                                                       gggCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAg                                                <  2:3515002/150‑1 (MQ=255)
                                                                                                       gggCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTAATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAg                                                >  1:3818614/1‑150 (MQ=255)
                                                                                                          ccaccaGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCct                                             <  2:38388/150‑1 (MQ=255)
                                                                                                              caGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTc                                         >  2:2068915/1‑150 (MQ=255)
                                                                                                                  aTTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCgcgc                                     >  2:2914896/1‑150 (MQ=255)
                                                                                                                     tCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGc                                  <  2:1527320/150‑1 (MQ=255)
                                                                                                                      cGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCg                                 >  1:1483477/1‑150 (MQ=255)
                                                                                                                       gTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCgg                                >  1:2750546/1‑150 (MQ=255)
                                                                                                                         ccGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAg                              >  1:1113383/1‑150 (MQ=255)
                                                                                                                           ggATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAgcg                            >  1:4000367/1‑150 (MQ=255)
                                                                                                                            gATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAgcgc                           <  1:4394922/150‑1 (MQ=255)
                                                                                                                             aTTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAgcgcg                          >  2:1238524/1‑150 (MQ=255)
                                                                                                                               tAATGCGGAAAAAACTGGCCATCGCCGGATCGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGTCTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAATGTATTTAGCCTCTTCGCGCAGCGGAGCGCGCa                        <  1:357126/150‑1 (MQ=255)
                                                                                                                                 aTGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCAGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCaaa                      >  2:2396959/1‑150 (MQ=255)
                                                                                                                                  tGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAAc                     <  2:3248686/150‑1 (MQ=255)
                                                                                                                                  tGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAAc                     <  1:2079640/150‑1 (MQ=255)
                                                                                                                                   gCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACg                    >  2:2896481/1‑150 (MQ=255)
                                                                                                                                       aaaaaaCTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGctct                >  1:1583528/1‑150 (MQ=255)
                                                                                                                                          aaaCTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGcc             >  1:2144499/1‑150 (MQ=255)
                                                                                                                                           aaCTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCa            >  1:2926916/1‑150 (MQ=255)
                                                                                                                                           aaCTGGACATCGCCGCAACGGCTTTGCAGCTGACATTCATAAATGTAGTCGGGACTAACAGGCGGACAACTTCGCCTCCAAGAGTGACTCGCGAAGTAAAACCAAAGAATTTAGACACTTCGCGCAGCGGAGAGCGCAAAAGCTCTGCCa            >  2:2408087/1‑150 (MQ=255)
                                                                                                                                            aCTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAg           >  1:2042811/1‑150 (MQ=255)
                                                                                                                                              tGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTa         <  2:3905016/150‑1 (MQ=255)
                                                                                                                                                gCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAg       >  1:1655108/1‑150 (MQ=255)
                                                                                                                                                gCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAg       >  2:2161745/1‑150 (MQ=255)
                                                                                                                                                 ccATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGAAAGt      >  2:4593258/1‑150 (MQ=255)
                                                                                                                                                   aTCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCa    >  2:2830140/1‑150 (MQ=255)
                                                                                                                                                     cgccgcAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAAc  >  2:1102336/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTATTACCCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAAC  >  CP009273/1253590‑1253888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑