BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,284,545	A→C	31.8%	L15R (CTA→CGA)	ychJ ←	UPF0225 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,284,545	0	A	C	31.8%	252.6 / 295.7	261	L15R (CTA→CGA)	ychJ	UPF0225 family protein
Reads supporting (aligned to +/- strand): ref base A (87/91); new base C (38/45); total (125/136)
Fisher's exact test for biased strand distribution p-value = 6.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 267 total aligned reads displayed.

CGGCACGTAACGCCGCTGCTCCACAAGAGGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCG  >  CP009273/1284396‑1284694

cGGCACGTAACGCCGCTGCTCCACAAGAGGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATa                                                                                                                                                       <  1:3488816/150‑1 (MQ=255)

cGGCACGTAACGCCGCTGCTCCACAAGAGGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATa                                                                                                                                                       <  2:3488929/150‑1 (MQ=255)

 ggCACGTAACGCCGCTGCTCCACAAGAGGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAg                                                                                                                                                      >  2:4202136/1‑150 (MQ=255)

 ggCACGTAACGCCGCTGCTCCACAAGAGGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAg                                                                                                                                                      >  2:843427/1‑150 (MQ=255)

 ggCACGTAACGCCGCTGCTCCACAAGAGGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAg                                                                                                                                                      <  2:1728459/150‑1 (MQ=255)

   cACGTAACGCCGCTGCTCCACAAGAGGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATCGGc                                                                                                                                                    <  2:2518540/150‑1 (MQ=255)

   cACGTAACGCCGCTGCTCCACAAGAGGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGc                                                                                                                                                    <  2:1031257/150‑1 (MQ=255)

     cGTAACGCCGCTGCTCCACAAGAGGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCta                                                                                                                                                  <  1:3031136/150‑1 (MQ=255)

      gTAACGCCGCTGCTCCACAAGAGGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCtat                                                                                                                                                 >  1:1023676/1‑150 (MQ=255)

      gTAACGCCGCTGCTACACAAGACGGATGCCAGGACTTTATTATATAATCTGCGTCTTGCATCACAAAAGCGCAGTATCGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATACGGGTGGCAACATCGGCtat                                                                                                                                                 <  2:341839/150‑1 (MQ=255)

       tAACGCCGCTGCTCCACAAGAGGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATCGGCtata                                                                                                                                                <  1:109395/150‑1 (MQ=255)

       tAACGCCGCTGCTCCACAAGAGGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCtata                                                                                                                                                <  2:2443346/150‑1 (MQ=255)

        aaCGCCGCTGCTCCACAAGAGGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCACCATAGGCTATAc                                                                                                                                               >  1:2426048/1‑150 (MQ=255)

        aaCGCCGCTGCTCCACAAGAGGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATAc                                                                                                                                               >  2:2451328/1‑150 (MQ=255)

         aCGCCGCTGCTCCACAAGAGGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACt                                                                                                                                              >  1:417184/1‑150 (MQ=255)

           gccgcTGCTCCACAAGAGGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATCGGCTATACTCg                                                                                                                                            >  2:1337814/1‑150 (MQ=255)

             cgcTGCTCCACAAGAGGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATCGGCTATACTCGAc                                                                                                                                          <  2:3352431/150‑1 (MQ=255)

              gctgctCCACACGAGGGATGCCAGGTCTTTATTAACTAATCTGCGTCTTTCATCACAAAAGAGCAGTATCGCGAACGCATGATATGTTCTTGATCAGGTGCAAACTTTTCACCAGACACATAAGGGTTGCAACATAGGCTTTACTCGACa                                                                                                                                         <  2:4311131/150‑1 (MQ=255)

              gctgctCCACAAGAGGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACa                                                                                                                                         >  1:760697/1‑150 (MQ=255)

              gctgctCCACAAGAGGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACa                                                                                                                                         <  1:4441831/150‑1 (MQ=255)

              gctgctCCACAAGAGGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACa                                                                                                                                         >  2:1790876/1‑150 (MQ=255)

                 gctCCACAAGAGGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCa                                                                                                                                      <  2:3433621/150‑1 (MQ=255)

                       caAGAGGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATCGGCTATACTCGACAGCACTACca                                                                                                                                <  1:1898702/150‑1 (MQ=255)

                         agagGTATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATCGGCTATACTCGACAGCACTACcaca                                                                                                                              >  2:3933333/1‑150 (MQ=255)

                         agagGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACcaca                                                                                                                              >  1:2018606/1‑150 (MQ=255)

                              gATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATCGGCTATACTCGACAGCACTACCACAGGGAc                                                                                                                         <  2:1815776/150‑1 (MQ=255)

                                tGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACaa                                                                                                                       <  2:2164270/150‑1 (MQ=255)

                                  ccAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATCGGCTATACTCGACAGCACTACCACAGGGACAAAg                                                                                                                     <  2:1191035/150‑1 (MQ=255)

                                  ccAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAg                                                                                                                     >  2:4592911/1‑150 (MQ=255)

                                   cAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATCGGCTATACTCGACAGCACTACCACAGGGACAAAGc                                                                                                                    >  2:3781336/1‑150 (MQ=255)

                                    aGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCt                                                                                                                   <  1:4381362/150‑1 (MQ=255)

                                           tattaAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGacac                                                                                                            >  2:1667646/1‑150 (MQ=255)

                                              taAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATCGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACaaa                                                                                                         >  2:339427/1‑150 (MQ=255)

                                               aaaTAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAaat                                                                                                        <  1:3973045/150‑1 (MQ=255)

                                                 ataatCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAaataa                                                                                                      >  1:3070544/1‑150 (MQ=255)

                                                     tCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATCGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAAtctc                                                                                                  <  2:4325162/150‑1 (MQ=255)

                                                        gCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCt                                                                                               >  2:2863316/1‑150 (MQ=255)

                                                         cGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTg                                                                                              >  1:780223/1‑150 (MQ=255)

                                                            cTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGaa                                                                                           >  2:4434989/1‑150 (MQ=255)

                                                              tgaaTCACAAAAGCGCAGTAACGCGAACGCATGAGATTTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTTGCAACATAGTCTATACTCGACAGCACTACCACAGGGACAAATCTGAGACACAAATAATCTCCCTGGAAAc                                                                                         <  1:508914/147‑1 (MQ=255)

                                                                 aTCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAAt                                                                                      >  1:1239460/1‑150 (MQ=255)

                                                                  tCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATa                                                                                     >  2:1759805/1‑150 (MQ=255)

                                                                   cacaAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATaa                                                                                    >  2:324422/1‑150 (MQ=255)

                                                                   cacaAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATaa                                                                                    >  1:550014/1‑150 (MQ=255)

                                                                    acaAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATCGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAAc                                                                                   >  2:2984197/1‑150 (MQ=255)

                                                                    acaAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATacc                                                                                   <  1:4202136/150‑3 (MQ=255)

                                                                    acaAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAAc                                                                                   <  1:1415640/150‑1 (MQ=255)

                                                                     caAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATCGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACg                                                                                  >  1:2110066/1‑150 (MQ=255)

                                                                      aaaaGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATCGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACgg                                                                                 >  1:2952520/1‑150 (MQ=255)

                                                                      aaaaGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACgg                                                                                 >  1:2729441/1‑150 (MQ=255)

                                                                      aaaaGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACgg                                                                                 <  2:417184/150‑1 (MQ=255)

                                                                        aaGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATCGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCg                                                                               >  1:3618734/1‑150 (MQ=255)

                                                                              aGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATCGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTaa                                                                         >  2:2012994/1‑150 (MQ=255)

                                                                                tAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAAcc                                                                       <  2:3978589/150‑1 (MQ=255)

                                                                                 aaCGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATCGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCg                                                                      >  1:1563774/1‑150 (MQ=255)

                                                                                 aaCGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCg                                                                      <  2:4605518/150‑1 (MQ=255)

                                                                                 aaCGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCg                                                                      <  1:1540108/150‑1 (MQ=255)

                                                                                   cgcgAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGcc                                                                    <  2:2733385/150‑1 (MQ=255)

                                                                                      gAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATCGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGa                                                                 >  1:1621183/1‑150 (MQ=255)

                                                                                        aCGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATCGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGt                                                               <  1:2326469/150‑1 (MQ=255)

                                                                                            aTGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCa                                                           <  2:4518976/150‑1 (MQ=255)

                                                                                              gagaTGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACt                                                         <  2:290455/150‑1 (MQ=255)

                                                                                                 aTGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATg                                                      >  1:625259/1‑150 (MQ=255)

                                                                                                 aTGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATg                                                      <  2:4257039/150‑1 (MQ=255)

                                                                                                 aTGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATg                                                      >  1:3679457/1‑150 (MQ=255)

                                                                                                 aTGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATg                                                      >  1:3620124/1‑150 (MQ=255)

                                                                                                   gTTCTGGATCAGGTGCATCCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGtt                                                    <  1:3429337/150‑1 (MQ=255)

                                                                                                       tGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAAcc                                                <  1:4440823/150‑1 (MQ=255)

                                                                                                       tGGATAAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACAACCACTGGGACAAAGCTGAGACACAAATAATCTCCCAGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGGTAAcc                                                >  2:573620/1‑150 (MQ=255)

                                                                                                        ggATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATCGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCg                                               >  2:1939929/1‑150 (MQ=255)

                                                                                                          aTCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATCGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAg                                             <  1:3781336/150‑1 (MQ=255)

                                                                                                            cAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATCGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACACTCACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCa                                           <  2:3546889/150‑1 (MQ=255)

                                                                                                            cAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATCGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCa                                           <  1:4223387/150‑1 (MQ=255)

                                                                                                            cAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCa                                           <  2:92705/150‑1 (MQ=255)

                                                                                                            cAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCa                                           >  1:1179357/1‑150 (MQ=255)

                                                                                                                 gCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGATACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGc                                      <  1:2451328/150‑1 (MQ=255)

                                                                                                                 gCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTACCCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGc                                      <  1:726488/150‑1 (MQ=255)

                                                                                                                   aaCCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGa                                    >  1:285997/1‑150 (MQ=255)

                                                                                                                     ccTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGAtg                                  >  1:3404627/1‑150 (MQ=255)

                                                                                                                       ttttCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGAtgtg                                <  2:3743124/150‑1 (MQ=255)

                                                                                                                                gACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAACTAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGAtt                       <  2:1538262/150‑1 (MQ=255)

                                                                                                                                gACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGAtt                       <  2:1538420/150‑1 (MQ=255)

                                                                                                                                gACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGAtt                       >  2:4418460/1‑150 (MQ=255)

                                                                                                                                   acaTAAGGGTGGCAACATCTGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACTGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCa                    <  1:4455054/150‑1 (MQ=255)

                                                                                                                                     aTAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCAtt                  >  2:4148533/1‑150 (MQ=255)

                                                                                                                                       aaGGGTGGCAACATCGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTcc                >  1:932550/1‑150 (MQ=255)

                                                                                                                                       aaGGGTGGCAACATCGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTcc                <  2:1474470/150‑1 (MQ=255)

                                                                                                                                          ggTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAgg             <  2:4143677/150‑1 (MQ=255)

                                                                                                                                           gTGGCAACATCGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAggg            <  2:3652626/150‑1 (MQ=255)

                                                                                                                                            tGGCAACATCGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAgggg           >  1:1963988/1‑150 (MQ=255)

                                                                                                                                            tGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAgggg           >  1:2775644/1‑150 (MQ=255)

                                                                                                                                            tGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAgggg           >  1:1453161/1‑150 (MQ=255)

                                                                                                                                             ggCAACATCGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGa          >  1:2899996/1‑150 (MQ=255)

                                                                                                                                             ggCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGa          >  2:1345363/1‑150 (MQ=255)

                                                                                                                                             ggCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGa          >  2:3809433/1‑150 (MQ=255)

                                                                                                                                               cAACATAGGCTATTCTCGACAGCACTACCACAGGGAAAAAGCTGTGACACAAATATTCTCCCTGGAAACAATAACGGAGTATTAACCGCCTGAGTAGCACTATGTGAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAAt        <  2:1996662/150‑1 (MQ=255)

                                                                                                                                                aaCATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACACTAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATc       <  2:2358797/150‑1 (MQ=255)

                                                                                                                                                  cATCGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCtt     <  2:1621183/150‑1 (MQ=255)

                                                                                                                                                  cATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCtt     <  2:2002783/150‑1 (MQ=255)

                                                                                                                                                     aGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCg  >  1:2563055/1‑150 (MQ=255)

CGGCACGTAACGCCGCTGCTCCACAAGAGGGATGCCAGGTCTTTATTAAATAATCTGCGTCTTGCATCACAAAAGCGCAGTAACGCGAACGCATGAGATGTTCTGGATCAGGTGCAACCTTTTCACCAGACACATAAGGGTGGCAACATAGGCTATACTCGACAGCACTACCACAGGGACAAAGCTGAGACACAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCG  >  CP009273/1284396‑1284694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`