Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,322,140 T→A 100% T159S (ACG→TCG)  btuR ← cob(I)yrinic acid a,c‑diamide adenosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,322,1400TA98.5% 956.3 / 0.9 273T159S (ACG→TCG) btuRcob(I)yrinic acid a,c‑diamide adenosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base A (145/123);  total (147/126)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 275 total aligned reads displayed.

TGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTT  >  CP009273/1321993‑1322286
                                                                                                                                                   |                                                                                                                                                  
tGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCgaga                                                                                                                                                  <  2:4496494/150‑1 (MQ=255)
tGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCgaga                                                                                                                                                  >  2:1696713/1‑150 (MQ=255)
 gCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGAt                                                                                                                                                 >  1:3133289/1‑150 (MQ=255)
     gACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATc                                                                                                                                             <  2:33839/150‑1 (MQ=255)
       cGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCAc                                                                                                                                           <  2:439215/150‑1 (MQ=255)
       cGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCAc                                                                                                                                           <  2:3022628/150‑1 (MQ=255)
        gTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCAcc                                                                                                                                          <  2:2749096/150‑1 (MQ=255)
         tCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCg                                                                                                                                         >  2:3352445/1‑150 (MQ=255)
            tatCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCt                                                                                                                                      <  2:2381217/150‑1 (MQ=255)
               ccGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGtt                                                                                                                                   >  2:2881274/1‑150 (MQ=255)
                cGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTg                                                                                                                                  <  2:1554102/150‑1 (MQ=255)
                   cATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGAtg                                                                                                                               <  1:881010/150‑1 (MQ=255)
                          aCAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGt                                                                                                                        >  1:1849791/1‑150 (MQ=255)
                           cAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTTTGTTGATGTGGACGtt                                                                                                                       >  1:1032223/1‑150 (MQ=255)
                             gATTAATAATCGCTCCCTATCTGCGCTTTGACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCa                                                                                                                     <  2:2156263/150‑1 (MQ=255)
                             gATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCa                                                                                                                     <  1:3600025/150‑1 (MQ=255)
                              aTTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCAt                                                                                                                    >  1:3583217/1‑150 (MQ=255)
                              aTTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCAt                                                                                                                    >  1:726660/1‑150 (MQ=255)
                                taataaTCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCAttt                                                                                                                  >  2:2486305/1‑150 (MQ=255)
                                taataaTCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCAttt                                                                                                                  >  2:1358115/1‑150 (MQ=255)
                                 aataatCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTa                                                                                                                 <  2:3966552/150‑1 (MQ=255)
                                 aataatCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTa                                                                                                                 <  2:3966401/150‑1 (MQ=255)
                                    aatCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACg                                                                                                              <  2:656315/150‑1 (MQ=255)
                                      tCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGcc                                                                                                            <  1:1453594/150‑1 (MQ=255)
                                        gATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTg                                                                                                          >  1:2999558/1‑150 (MQ=255)
                                        gATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGACTGTTGATGTGGACGTTCATTTAACGCCTg                                                                                                          >  2:3139160/1‑150 (MQ=255)
                                         aTCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGc                                                                                                         >  2:707827/1‑150 (MQ=255)
                                         aTCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGc                                                                                                         >  1:200555/1‑150 (MQ=255)
                                             cTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGcacca                                                                                                     >  1:563679/1‑150 (MQ=255)
                                                 cTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCActtc                                                                                                 <  2:163867/150‑1 (MQ=255)
                                                  tGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCActtct                                                                                                >  2:1092573/1‑150 (MQ=255)
                                                  tGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCActtct                                                                                                >  1:387313/1‑150 (MQ=255)
                                                   gcgcTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCActtctt                                                                                               >  2:2128560/1‑150 (MQ=255)
                                                   gcgcTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCActtctt                                                                                               <  2:2543614/150‑1 (MQ=255)
                                                   gcgcTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCActtctt                                                                                               <  1:2800392/150‑1 (MQ=255)
                                                     gcTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTcc                                                                                             <  1:3641749/150‑1 (MQ=255)
                                                     gcTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTcc                                                                                             <  1:118397/150‑1 (MQ=255)
                                                      cTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCa                                                                                            >  1:1058465/1‑150 (MQ=255)
                                                          tACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTgg                                                                                        <  1:2487717/150‑1 (MQ=255)
                                                          tACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTgg                                                                                        >  2:4374796/1‑150 (MQ=255)
                                                           acacCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGc                                                                                       >  1:4308305/1‑150 (MQ=255)
                                                           acacCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGc                                                                                       >  2:4603848/1‑150 (MQ=255)
                                                             acCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCaa                                                                                     >  1:541660/1‑150 (MQ=255)
                                                             acCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCaa                                                                                     >  1:2115254/1‑150 (MQ=255)
                                                             acCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCaa                                                                                     <  2:1195443/150‑1 (MQ=255)
                                                              ccGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCaaa                                                                                    >  1:4218992/1‑150 (MQ=255)
                                                               cGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAAt                                                                                   <  2:3640223/150‑1 (MQ=255)
                                                               cGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAAt                                                                                   >  2:1269892/1‑150 (MQ=255)
                                                                ggCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATa                                                                                  <  1:409738/150‑1 (MQ=255)
                                                                  cATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGt                                                                                >  1:2400344/1‑150 (MQ=255)
                                                                  cATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGt                                                                                <  1:812096/150‑1 (MQ=255)
                                                                  cATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGt                                                                                <  1:3629663/150‑1 (MQ=255)
                                                                    tCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCa                                                                              <  2:572874/150‑1 (MQ=255)
                                                                    tCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCa                                                                              >  1:1586997/1‑150 (MQ=255)
                                                                        aCGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACg                                                                          >  2:539114/1‑150 (MQ=255)
                                                                            aTGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGccac                                                                      <  2:3429359/150‑1 (MQ=255)
                                                                               tttGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCat                                                                   <  1:603453/150‑1 (MQ=255)
                                                                                 tGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCatat                                                                 >  1:1795564/1‑150 (MQ=255)
                                                                                  gACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCAtata                                                                <  2:419981/150‑1 (MQ=255)
                                                                                   aCGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATAc                                                               <  2:549909/150‑1 (MQ=255)
                                                                                      gggCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTc                                                            <  1:3759547/150‑1 (MQ=255)
                                                                                       ggCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCa                                                           >  1:3236566/1‑150 (MQ=255)
                                                                                       ggCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGACa                                                           >  1:4404412/1‑150 (MQ=255)
                                                                                        gCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAg                                                          <  1:4008613/150‑1 (MQ=255)
                                                                                           tAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTtc                                                       <  1:788400/150‑1 (MQ=255)
                                                                                                 aCTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAg                                                 >  2:813222/1‑150 (MQ=255)
                                                                                                   ttACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCa                                               >  2:850987/1‑150 (MQ=255)
                                                                                                    tACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCaa                                              <  1:2562350/150‑1 (MQ=255)
                                                                                                       cGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAc                                           >  1:4453462/1‑150 (MQ=255)
                                                                                                            cTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCatat                                      <  2:2457412/150‑1 (MQ=255)
                                                                                                             tGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATc                                     >  2:2588906/1‑150 (MQ=255)
                                                                                                             tGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATc                                     <  2:200574/150‑1 (MQ=255)
                                                                                                               ccAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATGATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCa                                   >  1:1413151/1‑150 (MQ=255)
                                                                                                                 aGTTCTAGAATATCCCGATGACAAACACGACCCGAGATAATCACCGTCTGTTGATGTGGTCGTTCATTTAACGCCTGCACCACTTCTTCAAGTTGCTAATAGTCATACGCCACCATATACGTCAGTTCTTCAAGCAAAACCATATCCatt                                 <  2:270977/150‑3 (MQ=255)
                                                                                                                   ttCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGa                               <  1:3291150/150‑1 (MQ=255)
                                                                                                                    tCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAg                              >  2:4586345/1‑150 (MQ=255)
                                                                                                                    tCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAg                              >  1:2063782/1‑150 (MQ=255)
                                                                                                                     cAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAgg                             <  1:3489097/150‑1 (MQ=255)
                                                                                                                        gAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAAt                          >  1:3423780/1‑150 (MQ=255)
                                                                                                                         aaTATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATc                         >  2:2828174/1‑150 (MQ=255)
                                                                                                                          atatCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCa                        <  1:1696713/150‑1 (MQ=255)
                                                                                                                          atatCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCa                        <  2:1334617/150‑1 (MQ=255)
                                                                                                                          atatCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCa                        <  2:3658827/150‑1 (MQ=255)
                                                                                                                           tatCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCag                       <  1:2881274/150‑1 (MQ=255)
                                                                                                                              cccGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCagcaa                    >  2:4126784/1‑150 (MQ=255)
                                                                                                                              cccGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCagcaa                    >  2:3082322/1‑150 (MQ=255)
                                                                                                                              cccGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCagcaa                    >  1:4575664/1‑150 (MQ=255)
                                                                                                                              cccGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCagcaa                    <  1:239214/150‑1 (MQ=255)
                                                                                                                               ccGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCagcaag                   >  1:4482845/1‑150 (MQ=255)
                                                                                                                                   tGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATc               >  2:1612209/1‑150 (MQ=255)
                                                                                                                                    gACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACTTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATcc              >  2:1949023/1‑150 (MQ=255)
                                                                                                                                    gACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATcc              <  2:3170137/150‑1 (MQ=255)
                                                                                                                                       aaCCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCt           >  2:3856435/1‑150 (MQ=255)
                                                                                                                                       aaCCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCt           >  1:1246116/1‑150 (MQ=255)
                                                                                                                                             gACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCAt     <  2:2234653/150‑1 (MQ=255)
                                                                                                                                             gACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCAt     >  1:1393346/1‑150 (MQ=255)
                                                                                                                                             gACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCAt     >  1:1966307/1‑150 (MQ=255)
                                                                                                                                              aCCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATg    >  1:4177778/1‑150 (MQ=255)
                                                                                                                                               cccGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGATTTGCATGt   >  1:2212847/1‑150 (MQ=255)
                                                                                                                                                ccGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGtt  <  1:3706686/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGCCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTT  >  CP009273/1321993‑1322286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑