BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,341,061	C→A	67.7%	Y3* (TAC→TAA)	ymiB →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,341,061	0	C	A	67.7%	228.6 / 266.7	235	Y3* (TAC→TAA)	ymiB	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (36/40); new base A (65/94); total (101/134)
Fisher's exact test for biased strand distribution p-value = 3.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 238 total aligned reads displayed.

AAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCA  >  CP009273/1340912‑1341210

aaaCATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAc                                                                                                                                                        <  2:2796999/150‑1 (MQ=255)

  aCATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACAt                                                                                                                                                      >  1:1753628/1‑150 (MQ=255)

        ctgctTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAAtt                                                                                                                                                <  1:2645633/150‑1 (MQ=255)

         tgctTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTa                                                                                                                                               <  2:194111/150‑1 (MQ=255)

          gctTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTaa                                                                                                                                              <  2:3087293/150‑1 (MQ=255)

          gctTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTaa                                                                                                                                              <  2:320385/150‑1 (MQ=255)

            tttCCGTCAACCGACAGTATGTATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGTGTCCGCGGTTAAATAAAATGAACAACATGACTTACATGAAATTAAcg                                                                                                                                            <  1:2126035/150‑1 (MQ=255)

              tCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAAcggc                                                                                                                                          <  1:1122787/150‑1 (MQ=255)

                cGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAAcggcgg                                                                                                                                        >  2:4404725/1‑150 (MQ=255)

                     aCCGCCAGTATGGATTGTGCGATCCGTGAAAGTTGTACAACGTGGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCGGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTaaa                                                                                                                                   <  1:847709/150‑1 (MQ=255)

                     aCCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTaaa                                                                                                                                   >  2:1521229/1‑150 (MQ=255)

                      ccGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAAc                                                                                                                                  <  1:3084243/150‑1 (MQ=255)

                      ccGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAAc                                                                                                                                  >  1:2569052/1‑150 (MQ=255)

                       cGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACg                                                                                                                                 <  2:3459088/150‑1 (MQ=255)

                       cGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACg                                                                                                                                 >  2:2639179/1‑150 (MQ=255)

                       cGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACg                                                                                                                                 <  2:3293757/150‑1 (MQ=255)

                        gCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGc                                                                                                                                >  2:926385/1‑150 (MQ=255)

                          cattATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGAATCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑t                                                                                                                             <  2:109789/147‑1 (MQ=255)

                          cAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑t                                                                                                                             >  2:1168858/1‑150 (MQ=255)

                          cAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGGTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCag                                                                                                                              >  2:1168864/1‑149 (MQ=255)

                            gTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTg                                                                                                                           <  1:1442579/150‑1 (MQ=255)

                            gTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTg                                                                                                                           >  1:442180/1‑150 (MQ=255)

                              aTGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGtt                                                                                                                         <  1:1612878/150‑1 (MQ=255)

                               tGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGttt                                                                                                                        <  1:2929386/150‑1 (MQ=255)

                                 gATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGttttt                                                                                                                      >  2:1816492/1‑150 (MQ=255)

                                   ttGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGttttttt                                                                                                                    >  2:3403087/1‑150 (MQ=255)

                                    tgtgCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGtttttttt                                                                                                                   <  2:239725/150‑1 (MQ=255)

                                    tgtgCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGtttttttt                                                                                                                   <  1:3895135/150‑1 (MQ=255)

                                     gtgCGATCCGAGATTGTTGTTCAACGTTGTTGACTCACTACCGGTTTTCTTGTTGCATATCCTCCGGTTAACAGCGTTGTCCGCGTTTAAATAACTTTAACAACATGACTTAATTGAATTTAACGGCGGCTAAACGCA‑TTGTTTTTTTta                                                                                                                  <  1:1967142/150‑1 (MQ=255)

                                     gtgCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTta                                                                                                                  >  2:1124758/1‑150 (MQ=255)

                                      tgCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGGA‑TTGTTTTTTTtat                                                                                                                 >  1:2583878/1‑150 (MQ=255)

                                      tgCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTtat                                                                                                                 <  1:2861093/150‑1 (MQ=255)

                                       gCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTtata                                                                                                                >  2:2154767/1‑150 (MQ=255)

                                       gCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTtata                                                                                                                >  2:4466434/1‑150 (MQ=255)

                                           tCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATAtatt                                                                                                            <  2:618449/150‑1 (MQ=255)

                                            ccGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATAtattt                                                                                                           <  2:2332553/150‑1 (MQ=255)

                                            ccGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATAtattt                                                                                                           <  1:413502/150‑1 (MQ=255)

                                             cGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTACCGGCGGCTAAACGCA‑TTGTTTTTTTTATAtattta                                                                                                          <  2:2477526/150‑1 (MQ=255)

                                              gagaAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATAtatttat                                                                                                         >  2:451589/1‑150 (MQ=255)

                                                gaAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATatttattt                                                                                                       <  1:1945896/150‑1 (MQ=255)

                                                 acaGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTg                                                                                                      >  1:493161/3‑150 (MQ=255)

                                                   aGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTa                                                                                                    <  1:1042289/150‑1 (MQ=255)

                                                    gTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTaa                                                                                                   <  1:245285/150‑1 (MQ=255)

                                                     ttGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAAt                                                                                                  <  2:2794499/150‑1 (MQ=255)

                                                       gTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATcc                                                                                                <  2:1655439/150‑1 (MQ=255)

                                                       gTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATcc                                                                                                <  2:2448720/150‑1 (MQ=255)

                                                       gTAAAACGTTGTGGACTCCCTAACGGTTTTCATTATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATcc                                                                                                <  1:3731250/150‑1 (MQ=255)

                                                        tACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCa                                                                                               >  2:1995967/1‑150 (MQ=255)

                                                        tACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAAATAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCa                                                                                               <  1:3405651/150‑1 (MQ=255)

                                                             cGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGtttt                                                                                          >  1:686146/1‑150 (MQ=255)

                                                             cGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGtttt                                                                                          >  1:2530172/1‑150 (MQ=255)

                                                              gTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTg                                                                                         <  2:2498862/150‑1 (MQ=255)

                                                               ttGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTgg                                                                                        >  1:2139324/1‑150 (MQ=255)

                                                                     aCTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAAc                                                                                  <  1:431227/150‑1 (MQ=255)

                                                                      cTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACg                                                                                 >  1:3018155/1‑150 (MQ=255)

                                                                       tCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGc                                                                                <  2:1230655/150‑1 (MQ=255)

                                                                          cTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAg                                                                             <  1:4481164/150‑1 (MQ=255)

                                                                          cTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAg                                                                             <  1:4480845/150‑1 (MQ=255)

                                                                          cTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAg                                                                             >  1:1253386/1‑150 (MQ=255)

                                                                           tAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGt                                                                            <  2:2265869/150‑1 (MQ=255)

                                                                            aaCGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑GTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGtt                                                                           >  2:3345345/1‑150 (MQ=255)

                                                                              cGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGtttt                                                                         >  1:1061145/1‑150 (MQ=255)

                                                                              cGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGtttt                                                                         <  1:4209482/150‑1 (MQ=255)

                                                                               ggTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTc                                                                        >  1:575399/1‑150 (MQ=255)

                                                                                 ttttCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCaa                                                                      <  2:316850/150‑1 (MQ=255)

                                                                                 ttttCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCaa                                                                      >  1:2361445/1‑150 (MQ=255)

                                                                                  tttCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCaaa                                                                     >  1:723228/1‑150 (MQ=255)

                                                                                  tttCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCaaa                                                                     >  2:1326885/1‑150 (MQ=255)

                                                                                   ttCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAAc                                                                    >  1:3205953/1‑150 (MQ=255)

                                                                                      atgatgCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGaa                                                                 <  2:2409585/150‑1 (MQ=255)

                                                                                          tgCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTc                                                             >  2:3776140/1‑150 (MQ=255)

                                                                                           gCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCa                                                            <  1:1384603/150‑1 (MQ=255)

                                                                                            cATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAg                                                           >  1:3725489/1‑150 (MQ=255)

                                                                                              tatCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGtt                                                         >  2:1835301/1‑150 (MQ=255)

                                                                                               atCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTa                                                        >  1:619058/1‑150 (MQ=255)

                                                                                                 cctccGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTaaa                                                      >  2:3835319/1‑150 (MQ=255)

                                                                                                  ctccGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTaaaa                                                     >  1:3628044/1‑150 (MQ=255)

                                                                                                   tccGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTaaaaa                                                    >  2:2662103/1‑150 (MQ=255)

                                                                                                       gTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCt                                                <  1:4205751/150‑1 (MQ=255)

                                                                                                            cAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTg                                           <  1:3856828/150‑1 (MQ=255)

                                                                                                            cAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTg                                           >  1:2953694/1‑150 (MQ=255)

                                                                                                              gCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCATGGa                                         >  2:4270648/1‑150 (MQ=255)

                                                                                                               cGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGat                                        <  2:3831698/150‑1 (MQ=255)

                                                                                                                 gAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGatat                                      <  2:79653/150‑1 (MQ=255)

                                                                                                                 gAGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGatat                                      <  2:3672239/150‑1 (MQ=255)

                                                                                                                  aGTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGAtata                                     <  1:1722814/150‑1 (MQ=255)

                                                                                                                   gTCCGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATaa                                    >  1:4002580/1‑150 (MQ=255)

                                                                                                                    tCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTTTTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATaa                                    <  1:1354166/150‑1 (MQ=255)

                                                                                                                     ccGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACg                                  <  1:918530/150‑1 (MQ=255)

                                                                                                                     ccGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACg                                  <  1:2764721/150‑1 (MQ=255)

                                                                                                                     ccGCGGTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACg                                  <  1:2764665/150‑1 (MQ=255)

                                                                                                                         ggTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAgg                              >  1:3433155/1‑150 (MQ=255)

                                                                                                                         ggTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAgg                              <  2:1113769/150‑1 (MQ=255)

                                                                                                                          gTTAAATAAAAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGACTGGATATAACGAAGGt                             >  1:4431268/1‑150 (MQ=255)

                                                                                                                             aaataaaAGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTaga                          >  2:3622861/1‑150 (MQ=255)

                                                                                                                                  aaaGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCggg                     >  2:953095/1‑150 (MQ=255)

                                                                                                                                  aaaGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCggg                     <  1:958834/150‑1 (MQ=255)

                                                                                                                                  aaaGCAAAAACAGGACTTAAATTAAATTAACGGCGGATAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATATGCCTGGATATAACGAAGGTAGAGCggg                     <  1:3282445/150‑1 (MQ=255)

                                                                                                                                   aaGGAACAACATGACTTAAATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCgggg                    <  2:4393467/150‑1 (MQ=255)

                                                                                                                                      gAAAAACATTACTTAAATTAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGaaa                 <  2:4145211/150‑1 (MQ=255)

AAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCA‑TTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCA  >  CP009273/1340912‑1341210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`