Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,384,386 T→A 35.4% H157L (CAC→CTC)  mpaA ← murein peptide amidase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,384,3860TA35.4% 305.4 / 311.7 263H157L (CAC→CTC) mpaAmurein peptide amidase A
Reads supporting (aligned to +/- strand):  ref base T (73/97);  new base A (41/52);  total (114/149)
Fisher's exact test for biased strand distribution p-value = 8.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 264 total aligned reads displayed.

TGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTC  >  CP009273/1384237‑1384534
                                                                                                                                                     |                                                                                                                                                    
tgcaatgcaaGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGt                                                                                                                                                      >  1:1711635/1‑150 (MQ=255)
      gcaaGTTCAGATCTGCACACCAACTGCCGACAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATg                                                                                                                                                <  1:998310/150‑1 (MQ=255)
      gcaaGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATg                                                                                                                                                >  2:2160455/1‑150 (MQ=255)
       caaGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATga                                                                                                                                               >  1:205383/1‑150 (MQ=255)
           ttCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGacc                                                                                                                                           >  2:3355391/1‑150 (MQ=255)
            tCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGacca                                                                                                                                          <  1:2937033/150‑1 (MQ=255)
               gATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCAccc                                                                                                                                       >  2:904202/1‑150 (MQ=255)
               gATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCAccc                                                                                                                                       >  2:3226558/1‑150 (MQ=255)
                aTCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCca                                                                                                                                      <  2:4603967/150‑1 (MQ=255)
                   tGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCcaggc                                                                                                                                   >  2:4116878/1‑150 (MQ=255)
                   tGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCcaggc                                                                                                                                   <  1:857481/150‑1 (MQ=255)
                   tGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCcaggc                                                                                                                                   <  2:2485290/150‑1 (MQ=255)
                    gCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGACTGAGACCACCcaggca                                                                                                                                  >  2:65146/1‑150 (MQ=255)
                    gCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCcaggca                                                                                                                                  <  1:661026/150‑1 (MQ=255)
                         ccAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTg                                                                                                                             <  2:3883515/150‑1 (MQ=255)
                          cAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGa                                                                                                                            <  2:2010256/150‑1 (MQ=255)
                            aCTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAAt                                                                                                                          <  1:442947/150‑1 (MQ=255)
                            aCTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAAt                                                                                                                          <  1:3289272/150‑1 (MQ=255)
                            aCTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAAt                                                                                                                          >  1:3854608/1‑150 (MQ=255)
                             cTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATg                                                                                                                         >  2:717070/1‑150 (MQ=255)
                               gCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCg                                                                                                                       <  2:577320/150‑1 (MQ=255)
                                 cGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGgt                                                                                                                     >  1:637063/1‑150 (MQ=255)
                                 cGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGgt                                                                                                                     <  1:1659146/150‑1 (MQ=255)
                                   aaaGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGgtgt                                                                                                                   >  1:1396489/1‑150 (MQ=255)
                                   aaaGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGgtgt                                                                                                                   <  2:1643292/150‑1 (MQ=255)
                                   aaaGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGgtgt                                                                                                                   <  1:4267184/150‑1 (MQ=255)
                                          cAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCt                                                                                                            <  1:762687/150‑1 (MQ=255)
                                             gCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGc                                                                                                         <  1:3096318/150‑1 (MQ=255)
                                               gTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGcac                                                                                                       >  2:2184943/1‑150 (MQ=255)
                                               gTTTCATAACCGACGCTGGTTAACAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGcac                                                                                                       >  2:2715414/1‑150 (MQ=255)
                                                tttCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGcaca                                                                                                      <  1:3934773/150‑1 (MQ=255)
                                                 ttCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAg                                                                                                     <  1:826473/150‑1 (MQ=255)
                                                  tCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAgc                                                                                                    >  2:2039604/1‑150 (MQ=255)
                                                   cATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAgcg                                                                                                   <  2:1464559/150‑1 (MQ=255)
                                                    aTAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAgcgc                                                                                                  >  1:924991/1‑150 (MQ=255)
                                                      aaCCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCt                                                                                                >  1:502252/1‑150 (MQ=255)
                                                      aaCCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCt                                                                                                >  1:471234/1‑150 (MQ=255)
                                                       aCCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTg                                                                                               >  1:3533298/1‑150 (MQ=255)
                                                       aCCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTg                                                                                               <  2:263202/150‑1 (MQ=255)
                                                        ccGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTgg                                                                                              <  2:3501734/150‑1 (MQ=255)
                                                        ccGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTgg                                                                                              <  2:1178041/150‑1 (MQ=255)
                                                        ccGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTgg                                                                                              <  2:3061995/150‑1 (MQ=255)
                                                         cGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTggg                                                                                             <  1:1110344/150‑1 (MQ=255)
                                                         cGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTggg                                                                                             >  1:942934/1‑150 (MQ=255)
                                                          gACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGt                                                                                            >  2:2626486/1‑150 (MQ=255)
                                                           aCGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGtt                                                                                           <  2:2411192/150‑1 (MQ=255)
                                                            cGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGttt                                                                                          <  2:3771552/150‑1 (MQ=255)
                                                            cGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGttt                                                                                          >  1:3713688/1‑150 (MQ=255)
                                                              cTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCa                                                                                        >  1:2977510/1‑150 (MQ=255)
                                                                ggTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAgg                                                                                      <  1:4297732/150‑1 (MQ=255)
                                                                  ttACCAAAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTCTAAGCTGGCACAGCGCCTGGGTTTCAGGtt                                                                                    <  1:2379282/150‑1 (MQ=255)
                                                                       agagGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAg                                                                               >  2:3852402/1‑150 (MQ=255)
                                                                       agagGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAg                                                                               >  1:1221309/1‑150 (MQ=255)
                                                                              aTTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGtt                                                                        <  1:2734448/150‑1 (MQ=255)
                                                                               ttCAAACGCCTGAGCCAGACATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGttt                                                                       <  2:225727/150‑1 (MQ=255)
                                                                                  aaaCGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTc                                                                    >  1:4136013/1‑150 (MQ=255)
                                                                                   aaCGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCa                                                                   >  2:4283725/1‑150 (MQ=255)
                                                                                   aaCGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCa                                                                   <  1:1033271/150‑1 (MQ=255)
                                                                                      gCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCt                                                                <  2:3632098/150‑1 (MQ=255)
                                                                                       ccTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTg                                                               >  1:4114918/1‑150 (MQ=255)
                                                                                        cTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGt                                                              <  1:1511163/150‑1 (MQ=255)
                                                                                         tGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTc                                                             >  2:4500980/1‑150 (MQ=255)
                                                                                          gAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCa                                                            <  1:562004/150‑1 (MQ=255)
                                                                                          gAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCa                                                            <  1:2880257/150‑1 (MQ=255)
                                                                                             ccagccaTTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTa                                                         >  2:1452487/1‑150 (MQ=255)
                                                                                             ccagccaATCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGAACGAGGAATGAGACCACCCAGGCAGCTTGAATGCGGTGTATAAGCTGCCACAGCGCCAGGGTTTCAGCTTCGGAGCCAGGTTAGTCACCTGTCAGtc                                                         >  1:3333910/1‑149 (MQ=255)
                                                                                                gccaTTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATa                                                      <  2:2055956/150‑1 (MQ=255)
                                                                                                gccaTTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATa                                                      <  2:2983751/150‑1 (MQ=255)
                                                                                                gccaTTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATa                                                      <  1:28557/150‑1 (MQ=255)
                                                                                                  caTTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACg                                                    >  2:1691223/1‑150 (MQ=255)
                                                                                                  caTTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACg                                                    <  2:2339894/150‑1 (MQ=255)
                                                                                                      cGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACAt                                                >  1:2311323/1‑150 (MQ=255)
                                                                                                      cGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACAt                                                <  1:224026/150‑1 (MQ=255)
                                                                                                        ccTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATcg                                              >  1:3583443/1‑150 (MQ=255)
                                                                                                          tAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATcgcg                                            >  2:4518825/1‑150 (MQ=255)
                                                                                                          tAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATcgcg                                            <  1:3484486/150‑1 (MQ=255)
                                                                                                            aTTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGtt                                          <  2:4324367/150‑1 (MQ=255)
                                                                                                             ttCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGttc                                         <  2:3419273/150‑1 (MQ=255)
                                                                                                             ttCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGttc                                         >  2:102368/1‑150 (MQ=255)
                                                                                                                gCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGttcttc                                      <  2:3854629/150‑1 (MQ=255)
                                                                                                                 cTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCa                                     <  2:1323227/150‑1 (MQ=255)
                                                                                                                 atgtgTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCa                                     >  2:3389697/2‑150 (MQ=255)
                                                                                                                      tCTGGGATCTTCAATACAGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAgcggc                                <  1:2398607/150‑1 (MQ=255)
                                                                                                                       cTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAgcggcg                               >  2:1603312/1‑150 (MQ=255)
                                                                                                                         gggATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCt                             >  2:287982/1‑150 (MQ=255)
                                                                                                                         gggATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCt                             <  1:4309650/150‑1 (MQ=255)
                                                                                                                         gggATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCt                             >  1:4435403/1‑150 (MQ=255)
                                                                                                                                    tACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGAt                  <  1:516786/150‑1 (MQ=255)
                                                                                                                                    tACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGAt                  <  1:2243028/150‑1 (MQ=255)
                                                                                                                                    tACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGAt                  <  1:2243193/150‑1 (MQ=255)
                                                                                                                                       aGGCCAGTGGATCGAGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATaaa               >  2:4455376/1‑150 (MQ=255)
                                                                                                                                         gCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACg             <  1:2722565/150‑1 (MQ=255)
                                                                                                                                            aGTGAATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTAGGAGCCAGGTTAGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGtt          >  2:3733577/1‑150 (MQ=255)
                                                                                                                                             gTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGttt         <  1:2533639/150‑1 (MQ=255)
                                                                                                                                              tGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTc        <  2:4209076/150‑1 (MQ=255)
                                                                                                                                              tGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTc        >  1:317817/1‑150 (MQ=255)
                                                                                                                                                gATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCAc      <  1:3420105/150‑1 (MQ=255)
                                                                                                                                                 aTCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCAcc     <  2:502306/150‑1 (MQ=255)
                                                                                                                                                  tCGTGGTCTGAGACCACACAGGCAGGTTGATTGCGGTGTATAAGCTGTCACAGCGCCTGGGTTTCAGGTTCGTAGCCAGGTTTGTCACCTGTCAGTAATACGACATAGCGTTCTTCTGCGGCGCTGTTCCAGCGATAAACGGTTTCTcct    <  2:817954/150‑1 (MQ=255)
                                                                                                                                                    gTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCAccttc  <  1:2184943/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTC  >  CP009273/1384237‑1384534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑