Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,386,008 G→T 100% Q35K (CAG→AAG)  ycjY ← S9 homolog non‑peptidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,386,0080GT97.5% 1043.2 / 15.9 319Q35K (CAG→AAG) ycjYS9 homolog non‑peptidase family protein
Reads supporting (aligned to +/- strand):  ref base G (2/6);  new base T (128/182);  total (131/188)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 323 total aligned reads displayed.

CTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGAT  >  CP009273/1385861‑1386154
                                                                                                                                                   |                                                                                                                                                  
 tAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTTAAGCCCCCCCCTGGGTGAGATAACACAATTTCCTtata                                                                                                                                                 >  1:2763255/1‑150 (MQ=255)
 tAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTtata                                                                                                                                                 <  1:867196/150‑1 (MQ=255)
     tGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAAAGCCCCCCCCAGGGGGGGATAACACAAATGCCTTATAATGa                                                                                                                                             >  1:35700/1‑150 (MQ=255)
     tGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCTGGGTGAGATAACACAATTTCCTTATACtta                                                                                                                                             >  1:3229872/1‑148 (MQ=255)
        cgcgGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGTGATAACACAATTGCCTTATACTGACgg                                                                                                                                          >  1:1791525/1‑150 (MQ=255)
        cgcgGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCGGGGTGAGATAACACAATTGCCTTATACTGACgg                                                                                                                                          >  1:4122591/1‑150 (MQ=255)
          cgGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGt                                                                                                                                        <  2:1661564/150‑1 (MQ=255)
          cgGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGGGAGATAACACAATTGCCTTATACTGACGGGt                                                                                                                                        >  1:2568638/1‑150 (MQ=255)
          cgGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCGTTAACGCCCCCAACTGGGTGAGATAACACAAATGCCTTATACTGACCGGt                                                                                                                                        >  1:1440294/1‑150 (MQ=255)
                 ccgccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTtcatca                                                                                                                                 <  2:1351430/150‑1 (MQ=255)
                  cgccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCaa                                                                                                                                <  1:3811826/150‑1 (MQ=255)
                    ccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAAt                                                                                                                              <  2:2894673/150‑1 (MQ=255)
                    ccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAAt                                                                                                                              >  2:1870467/1‑150 (MQ=255)
                    ccACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAAt                                                                                                                              >  2:3364578/1‑150 (MQ=255)
                     cACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCCCCAGGGGGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAAtt                                                                                                                             >  1:2764358/1‑150 (MQ=255)
                     cACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAAtt                                                                                                                             <  2:2328307/150‑1 (MQ=255)
                     cACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAAtt                                                                                                                             >  1:2925676/1‑150 (MQ=255)
                       cTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAAtttt                                                                                                                           <  2:3289689/150‑1 (MQ=255)
                       cTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAAtttt                                                                                                                           <  1:563927/150‑1 (MQ=255)
                        tttCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGTTAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTg                                                                                                                          <  2:5703/150‑1 (MQ=255)
                        tttCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTg                                                                                                                          <  2:389153/150‑1 (MQ=255)
                        tttCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTg                                                                                                                          <  1:4010242/150‑1 (MQ=255)
                         ttCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAAGTGCCGTATACTGACGGGTTTCATCAAATTTTgg                                                                                                                         >  2:3150865/1‑150 (MQ=255)
                           cACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAg                                                                                                                       <  1:897585/150‑1 (MQ=255)
                            aCCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAgg                                                                                                                      <  2:495207/150‑1 (MQ=255)
                            aCCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAAAGCCCCCACCGGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATAAAATTTTGGAgg                                                                                                                      >  1:131184/1‑150 (MQ=255)
                             ccTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAggg                                                                                                                     <  1:3882599/150‑1 (MQ=255)
                              cTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGACAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCTTCAAATTTTGGAGGGa                                                                                                                    <  1:2377426/150‑1 (MQ=255)
                                tGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGaaa                                                                                                                  <  1:1627540/150‑1 (MQ=255)
                                    aaGATGCTTCATAAGCAATAGTGACAAATCCCATTTCCGCCATTTTTTTGGCATAGGTACCGCCAGTGAGTTCTTTAACTCCCCCACCTGAGAGAGATAACACAAAACCCTAATACTAAAGCGTGTCATCAAATTTTGGAGGGAAATAGa                                                                                                              >  2:146663/1‑150 (MQ=255)
                                      gATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCTTTAAAGCCCCCCCCGGGGTGAGATAACACAAATGCCTTATACTGACGGGTTTCAACAAAATTTGGTGGGAAATAGAta                                                                                                            >  1:1596343/1‑149 (MQ=255)
                                         gCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACt                                                                                                         <  1:1121591/150‑1 (MQ=255)
                                            tCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCa                                                                                                      <  2:1891095/150‑1 (MQ=255)
                                            tCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCa                                                                                                      >  2:2622126/1‑150 (MQ=255)
                                              aTAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGa                                                                                                    <  1:4358207/150‑1 (MQ=255)
                                                aaGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACa                                                                                                  <  1:986980/150‑1 (MQ=255)
                                                aaGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGGTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACa                                                                                                  >  1:713942/1‑150 (MQ=255)
                                                 aGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTATTTAACGCCCCCACCTGGGTGAGATAACACCATTGCCTTATACTGACGGGTTTCATCAAATTTTGGTGGGAAATAGATCACTGCAGACaa                                                                                                 >  2:798799/1‑150 (MQ=255)
                                                  gCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACaaa                                                                                                <  1:1018106/150‑1 (MQ=255)
                                                    aaTAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAaga                                                                                              <  1:3020219/150‑1 (MQ=255)
                                                     aTAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTCGTTTACGCCCCCACCAGGGTGAGATAACACAAATGCCGTATAAAGACGGGTTTAATCAAATTTTGGGGGGAAATAGATAACTGCCGACAAagag                                                                                             >  1:3115258/1‑150 (MQ=255)
                                                       aGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGAt                                                                                           <  1:2647696/150‑1 (MQ=255)
                                                         tGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTTCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATgg                                                                                         >  2:2765250/1‑150 (MQ=255)
                                                         tGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATgg                                                                                         <  2:4377222/150‑1 (MQ=255)
                                                         tGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATgg                                                                                         >  2:3044418/1‑150 (MQ=255)
                                                         tGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCGGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATgg                                                                                         >  2:2931081/1‑150 (MQ=255)
                                                           aCAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGtt                                                                                       <  1:2288129/150‑1 (MQ=255)
                                                           aCAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGtt                                                                                       <  1:1477659/150‑1 (MQ=255)
                                                           aCAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGtt                                                                                       <  1:3035025/150‑1 (MQ=255)
                                                             aaaTCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTGTTTAACGCCCCCACCTGGGTGAGATAACACAAATTCCTTATAATGACGGGTTTGATAAAATTTTGTAGGGAAATAGATCACTGCAGACAAAGAGATGGTTgg                                                                                     >  1:2447615/1‑150 (MQ=255)
                                                               aTCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGat                                                                                   >  2:2688351/1‑150 (MQ=255)
                                                               aTCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGat                                                                                   <  2:4455595/150‑1 (MQ=255)
                                                               aTCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGGTCTTTAACCCCCCCACCAGGGTGAGATAACAAAAATTCCTTAAACTGACGGGTTTCATCAAAATTTGGAGGGAAATAGATCACAGCAGAAAAAGAGATGGTTGGaa                                                                                   >  2:2446016/1‑149 (MQ=255)
                                                                tCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGAAGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGatt                                                                                  >  1:4166021/1‑150 (MQ=255)
                                                                  ccTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGattat                                                                                <  1:2168399/150‑1 (MQ=255)
                                                                   cTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGattatt                                                                               <  2:2189222/150‑1 (MQ=255)
                                                                   cTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAAATTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGattatt                                                                               >  2:2279224/1‑150 (MQ=255)
                                                                    ttttCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTg                                                                              <  2:887445/150‑1 (MQ=255)
                                                                    ttttCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTATTTAAAGCCCCCACCTGGGTGTGATAACAAAATTTGCTTATAATTAAGGGTTTAAAAAAATTTTTGAGGGAAATATATAAAAGAAGAAAAAGATATTGTTGGatttttt                                                                              >  1:3481655/1‑149 (MQ=255)
                                                                     tttCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTGTGTTGTTTAAAGCCCCCACCAGGGGGAGATAACACAAATGCCTTATACTGACGGGGTTCAACAAAATTTTGAGGGAAATAGATCACAGCAGACAAAAAGATGGTTGGGTTATTGc                                                                             >  1:2340718/1‑150 (MQ=255)
                                                                       tCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGTGGGAAATAGATCACTGCCGACAAAGAGATAGTTGGATTATTGCTg                                                                           >  2:1931103/1‑150 (MQ=255)
                                                                        ccGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCGGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACAGCAGACAAAGAGATGGTTGGATTATTGCTGt                                                                          >  1:4577072/1‑150 (MQ=255)
                                                                         cGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGtt                                                                         <  1:2063268/150‑1 (MQ=255)
                                                                         cGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGtt                                                                         <  1:4473746/150‑1 (MQ=255)
                                                                          gCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTa                                                                        >  2:904614/1‑150 (MQ=255)
                                                                           ccAGTTTTTTGTCATAGGTTCCGGCCGTTTGTTCTTTATCGCCCCCACCTGGGTGAGATAACACAATTGACTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAg                                                                       <  1:1170334/150‑1 (MQ=255)
                                                                           ccAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCTGGGTGAGATAACACAATTGCCGTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAg                                                                       >  2:1639958/1‑150 (MQ=255)
                                                                           ccAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCTGGGTGAGATAACACAATTGCCGTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAg                                                                       >  2:3968501/1‑150 (MQ=255)
                                                                            cAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGt                                                                      <  2:3493217/150‑1 (MQ=255)
                                                                            cAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGt                                                                      <  2:870495/150‑1 (MQ=255)
                                                                             aGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTg                                                                     <  2:1815093/150‑1 (MQ=255)
                                                                                tttttGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAg                                                                  <  2:199599/150‑1 (MQ=255)
                                                                                 ttttGGCATAGGTTCCGGCCGTTTGTTCTTTAAAGCCCCCACCAGGGGGAGATAACACAATTTCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATAACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGc                                                                 >  1:201048/1‑150 (MQ=255)
                                                                                       cATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATAACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGGAGCTGACtt                                                           >  2:4045204/1‑150 (MQ=255)
                                                                                       cATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCAGGGTGAGATAACACAAATGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACtt                                                           >  1:3815764/1‑150 (MQ=255)
                                                                                             ttCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTtattat                                                     <  1:392843/150‑1 (MQ=255)
                                                                                             ttCCGGCCGTTTGTTATTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATAATGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACAGCAGACAAAGAGATGGTTTGATTATTGCTGTTAGTGAAGCTGACGTtattat                                                     >  2:1667197/1‑150 (MQ=255)
                                                                                               ccGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtc                                                   <  2:3691541/150‑1 (MQ=255)
                                                                                                cGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtca                                                  <  1:21921/150‑1 (MQ=255)
                                                                                                 ggCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATtcat                                                 >  2:3190657/1‑150 (MQ=255)
                                                                                                 ggCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAAATGCCTTATACTGACGGGTTTCATAAAATTTTGGAGGGAAATAGATCAATGCAGACAAAGAGATGGTTGGATTATTTCTGTTAGTTAAGCTGACTTTATTATtaat                                                 >  1:1935415/1‑150 (MQ=255)
                                                                                                      tttGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTc                                            <  1:3276590/150‑1 (MQ=255)
                                                                                                        tGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCg                                          >  2:4336128/1‑150 (MQ=255)
                                                                                                        tGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCg                                          >  2:4408076/1‑150 (MQ=255)
                                                                                                         gTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGt                                         <  1:1518965/150‑1 (MQ=255)
                                                                                                             tttAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCt                                     <  1:3303523/150‑1 (MQ=255)
                                                                                                             tttAACGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCt                                     <  2:4588636/150‑1 (MQ=255)
                                                                                                                 aCGCCCCCACCTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATg                                 >  2:1090658/1‑150 (MQ=255)
                                                                                                                           cTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCgg                       <  2:1404458/150‑1 (MQ=255)
                                                                                                                           cTGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCgg                       <  2:4135675/150‑1 (MQ=255)
                                                                                                                            tGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGt                      >  2:356420/1‑150 (MQ=255)
                                                                                                                            tGGGTGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGt                      <  2:1246626/150‑1 (MQ=255)
                                                                                                                                tGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGt                  <  2:1630465/150‑1 (MQ=255)
                                                                                                                                tGAGATAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGt                  >  1:4425445/1‑150 (MQ=255)
                                                                                                                                  agaTAACACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGttt                >  1:4260910/1‑150 (MQ=255)
                                                                                                                                      aaCACAATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAAc            >  2:650846/1‑150 (MQ=255)
                                                                                                                                         acaATTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGt         <  2:3598787/150‑1 (MQ=255)
                                                                                                                                            aTTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTgg      <  1:1870467/150‑1 (MQ=255)
                                                                                                                                            aTTGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTgg      <  1:3388982/150‑1 (MQ=255)
                                                                                                                                              tGCCTTATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTg    <  1:172104/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTAACTGACGCGGCTCGCCGCCACTTTCACCTTGATAAGATGCGTCATAAGCAATAGTGACAAATCCCTTTTCCGCCAGTTTTTTGGCATAGGTTCCGGCCGTTTGTTCTTTAACGCCCCCACCTGGGTGAGATAACACAATTGCCTGATACTGACGGGTTTCATCAAATTTTGGAGGGAAATAGATCACTGCAGACAAAGAGATGGTTGGATTATTGCTGTTAGTGAAGCTGACTTTATTATTCATCATTCCGTTCCTCATGGAGTTGTCGGTTCGTTTTAACGGTTGGTGAT  >  CP009273/1385861‑1386154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑