Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,552,629 C→A 90.2% D216Y (GAT→TAT)  ddpD ← D,D‑dipeptide permease system, ATP‑binding component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,552,6290CA90.2% 839.9 / 95.4 318D216Y (GAT→TAT) ddpDD,D‑dipeptide permease system, ATP‑binding component
Reads supporting (aligned to +/- strand):  ref base C (22/9);  new base A (174/112);  total (196/122)
Fisher's exact test for biased strand distribution p-value = 3.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.73e-01

Only 100 of 318 total aligned reads displayed.

CTCCATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCA  >  CP009273/1552482‑1552774
                                                                                                                                                   |                                                                                                                                                 
cTCCATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAAt                                                                                                                                                 <  1:3957156/150‑1 (MQ=255)
  ccATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATgg                                                                                                                                               >  1:3888451/1‑150 (MQ=255)
     tGTTCTGGTGCGCATTGCCGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCtg                                                                                                                                            >  2:4382146/1‑150 (MQ=255)
     tGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCtg                                                                                                                                            >  2:271291/1‑150 (MQ=255)
       ttCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCtgat                                                                                                                                          >  1:4230627/1‑150 (MQ=255)
        tCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCtgatg                                                                                                                                         >  2:1882228/1‑150 (MQ=255)
        tCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCtgatg                                                                                                                                         <  2:2652927/150‑1 (MQ=255)
          tGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGaa                                                                                                                                       >  1:3403252/1‑150 (MQ=255)
          tGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGaa                                                                                                                                       >  1:4426090/1‑150 (MQ=255)
            gTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACa                                                                                                                                     <  1:4561233/150‑1 (MQ=255)
               cgcATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGaa                                                                                                                                  >  1:2561620/1‑150 (MQ=255)
                gcATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAAc                                                                                                                                 <  1:938361/150‑1 (MQ=255)
                gcATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAAc                                                                                                                                 <  2:4518186/150‑1 (MQ=255)
                gcATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAAc                                                                                                                                 >  2:4517805/1‑150 (MQ=255)
                    tGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCa                                                                                                                             >  1:3538154/1‑150 (MQ=255)
                         caAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTcc                                                                                                                        >  2:3909745/1‑150 (MQ=255)
                          aaaCCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCa                                                                                                                       >  2:868417/1‑150 (MQ=255)
                          aaaCCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCa                                                                                                                       >  2:3660002/1‑150 (MQ=255)
                          aaaCCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCa                                                                                                                       >  2:3136518/1‑150 (MQ=255)
                            aCCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACt                                                                                                                     >  1:1939513/1‑150 (MQ=255)
                            aCCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACt                                                                                                                     >  1:851133/1‑150 (MQ=255)
                             ccAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTg                                                                                                                    >  2:4169046/1‑150 (MQ=255)
                             ccAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTg                                                                                                                    <  2:1290220/150‑1 (MQ=255)
                                        cGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCttt                                                                                                         <  1:4443191/150‑1 (MQ=255)
                                         ggATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTa                                                                                                        >  1:704335/1‑150 (MQ=255)
                                         ggATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTa                                                                                                        >  2:1197151/1‑150 (MQ=255)
                                         ggATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTa                                                                                                        <  1:1574108/150‑1 (MQ=255)
                                              ccGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGttt                                                                                                   <  2:3864930/150‑1 (MQ=255)
                                               cGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTa                                                                                                  >  2:1344364/1‑150 (MQ=255)
                                               cGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTa                                                                                                  >  1:2310760/1‑150 (MQ=255)
                                                ggggATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTaa                                                                                                 >  1:3022790/1‑150 (MQ=255)
                                                ggggATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTaa                                                                                                 >  1:4105175/1‑150 (MQ=255)
                                                  ggATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGc                                                                                               <  2:2844453/150‑1 (MQ=255)
                                                   gatgatGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCa                                                                                              >  1:818818/1‑150 (MQ=255)
                                                      gatgGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAAc                                                                                           <  1:2264884/150‑1 (MQ=255)
                                                       atgGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACg                                                                                          >  2:2791433/1‑150 (MQ=255)
                                                        tgGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGc                                                                                         <  1:1014893/150‑1 (MQ=255)
                                                           aTAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGt                                                                                      <  1:3751786/150‑1 (MQ=255)
                                                             aaCGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTAc                                                                                    >  2:4635906/1‑150 (MQ=255)
                                                                gTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTg                                                                                 <  2:2933104/150‑1 (MQ=255)
                                                                   tGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCaa                                                                              >  2:1440748/1‑150 (MQ=255)
                                                                      cGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTg                                                                           <  2:1989321/150‑1 (MQ=255)
                                                                      cGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTg                                                                           >  2:4311384/1‑150 (MQ=255)
                                                                      cGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTg                                                                           >  1:1475076/1‑150 (MQ=255)
                                                                       gTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGc                                                                          >  1:638773/1‑150 (MQ=255)
                                                                       gTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGc                                                                          >  2:2851360/1‑150 (MQ=255)
                                                                        tCACGCCGCTTTCAATTACGCTTACGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCa                                                                         >  2:2312853/1‑150 (MQ=255)
                                                                           cgccgcTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCg                                                                      <  1:2099928/150‑1 (MQ=255)
                                                                           cgccgcTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCg                                                                      <  2:576493/150‑1 (MQ=255)
                                                                            gccgcTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGt                                                                     >  2:4552813/1‑150 (MQ=255)
                                                                              cgcTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGa                                                                   <  2:4085017/150‑1 (MQ=255)
                                                                               gcTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGAc                                                                  >  2:873407/1‑150 (MQ=255)
                                                                               gcTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGAc                                                                  >  2:873486/1‑150 (MQ=255)
                                                                                 tttCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGt                                                                >  1:1849948/1‑150 (MQ=255)
                                                                                    cAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACCGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCACACGCAGTACCTGCAACTGCACCGTGACGTCCa                                                             >  2:3960444/1‑150 (MQ=255)
                                                                                     aaTTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAg                                                            <  2:2207626/150‑1 (MQ=255)
                                                                                     aaTTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAg                                                            >  1:2787776/1‑150 (MQ=255)
                                                                                      aTTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAgc                                                           <  1:486874/150‑1 (MQ=255)
                                                                                         aCGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGcc                                                        <  2:1109348/150‑1 (MQ=255)
                                                                                              tCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGt                                                   >  2:178380/1‑150 (MQ=255)
                                                                                               ccGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGACCCGCAGGTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTc                                                  <  2:2486870/150‑1 (MQ=255)
                                                                                               ccGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTc                                                  >  1:1068825/1‑150 (MQ=255)
                                                                                                   cATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGtt                                              <  1:2742281/150‑1 (MQ=255)
                                                                                                    aTACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTtc                                             >  1:3655356/1‑150 (MQ=255)
                                                                                                    aTACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTtc                                             >  1:3116413/1‑150 (MQ=255)
                                                                                                       cATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTtcgtc                                          <  1:3909760/150‑1 (MQ=255)
                                                                                                       cATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTtcgtc                                          <  2:3229991/150‑1 (MQ=255)
                                                                                                       cATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTtcgtc                                          <  2:4603797/150‑1 (MQ=255)
                                                                                                          cACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGc                                       >  1:1294513/1‑150 (MQ=255)
                                                                                                           aCGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCa                                      >  1:4324229/1‑150 (MQ=255)
                                                                                                           aCGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCa                                      >  2:1139987/1‑150 (MQ=255)
                                                                                                            cGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCaa                                     <  1:1765094/150‑1 (MQ=255)
                                                                                                             gTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCaat                                    >  2:2675697/1‑150 (MQ=255)
                                                                                                             gTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACATAACCGCAGTTCCACTGGCTCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCaat                                    >  2:3460703/1‑150 (MQ=255)
                                                                                                             gTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCaat                                    >  2:1135706/1‑150 (MQ=255)
                                                                                                              tAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCaata                                   >  1:3670630/1‑150 (MQ=255)
                                                                                                              tAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCaata                                   <  2:1779530/150‑1 (MQ=255)
                                                                                                              tAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCaata                                   >  1:2937561/1‑150 (MQ=255)
                                                                                                                aaCGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCaataat                                 <  1:2363253/150‑1 (MQ=255)
                                                                                                                aaCGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCaataat                                 >  1:4537718/1‑150 (MQ=255)
                                                                                                                aaCGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCaataat                                 >  2:4207341/1‑150 (MQ=255)
                                                                                                                 aCGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATc                                >  1:4293601/1‑150 (MQ=255)
                                                                                                                    cTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAAt                             <  2:552284/150‑1 (MQ=255)
                                                                                                                    cTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGATTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAAt                             >  1:324746/1‑150 (MQ=255)
                                                                                                                     tATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAAtt                            <  1:3618096/150‑1 (MQ=255)
                                                                                                                     tATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAAtt                            <  1:3618142/150‑1 (MQ=255)
                                                                                                                          cacaGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGc                       <  1:4517805/150‑1 (MQ=255)
                                                                                                                           acaGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTTCCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCt                      <  1:4008317/150‑1 (MQ=255)
                                                                                                                            caGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTc                     <  2:1712293/150‑1 (MQ=255)
                                                                                                                             aGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCg                    >  1:3981877/1‑150 (MQ=255)
                                                                                                                                tGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAg                 >  2:1601261/1‑150 (MQ=255)
                                                                                                                                     caccacGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGaa            >  2:2329182/1‑150 (MQ=255)
                                                                                                                                     caccacGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGaa            >  2:2627855/1‑150 (MQ=255)
                                                                                                                                           ggCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAg      <  1:2393927/150‑1 (MQ=255)
                                                                                                                                           ggCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAg      <  1:4169046/150‑1 (MQ=255)
                                                                                                                                            gCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAgc     <  2:290102/150‑1 (MQ=255)
                                                                                                                                              cATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAgcgc   >  2:452527/1‑150 (MQ=255)
                                                                                                                                              cATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAgcgc   >  1:2175078/1‑150 (MQ=255)
                                                                                                                                               atatAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCa  >  1:2468017/1‑150 (MQ=255)
                                                                                                                                               atatAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCa  >  1:624300/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CTCCATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCA  >  CP009273/1552482‑1552774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑