Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,591,853 C→A 15.6% pseudogene (392/5323 nt) yneO ← pseudogene, AidA homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,591,8530CA15.6% 922.0 / 98.5 281pseudogene (392/5323 nt)yneOpseudogene, AidA homolog
Reads supporting (aligned to +/- strand):  ref base C (134/103);  new base A (19/25);  total (153/128)
Fisher's exact test for biased strand distribution p-value = 1.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 281 total aligned reads displayed.

GGCGACGATTGAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTC  >  CP009273/1591705‑1592001
                                                                                                                                                    |                                                                                                                                                    
ggCGACGATTGAACTCGTGACGTAACCCTTATCCTTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCt                                                                                                                                                     <  2:219383/150‑1 (MQ=255)
ggCGACGATTGAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCt                                                                                                                                                     >  1:1560851/1‑150 (MQ=255)
ggCGACGATTGAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCt                                                                                                                                                     >  1:3889498/1‑150 (MQ=255)
ggCGACGATTGAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCt                                                                                                                                                     <  1:3488237/150‑1 (MQ=255)
     cgaTTGAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAAt                                                                                                                                                <  1:1068614/150‑1 (MQ=255)
     cgaTTGAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAAt                                                                                                                                                >  2:295294/1‑150 (MQ=255)
      gaTTGAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATa                                                                                                                                               >  1:2879503/1‑150 (MQ=255)
       aTTGAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAg                                                                                                                                              >  2:351521/1‑150 (MQ=255)
          gAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCt                                                                                                                                           >  1:4589664/1‑150 (MQ=255)
             cTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCa                                                                                                                                        >  1:4111246/1‑150 (MQ=255)
              tCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCAt                                                                                                                                       <  2:3907542/150‑1 (MQ=255)
                  gACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGAc                                                                                                                                   <  2:3056574/150‑1 (MQ=255)
                  gACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGAc                                                                                                                                   >  1:1898336/1‑150 (MQ=255)
                    cGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGt                                                                                                                                 >  2:790370/1‑150 (MQ=255)
                     gTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTg                                                                                                                                <  1:1063149/150‑1 (MQ=255)
                          ccTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCt                                                                                                                           >  2:4515511/1‑150 (MQ=255)
                          ccTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCt                                                                                                                           >  2:402157/1‑150 (MQ=255)
                           cTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCtt                                                                                                                          >  2:3252746/1‑150 (MQ=255)
                               tCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGt                                                                                                                      >  1:1522887/1‑150 (MQ=255)
                               tCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGt                                                                                                                      >  1:3386963/1‑150 (MQ=255)
                                ccGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGtt                                                                                                                     <  1:340969/150‑1 (MQ=255)
                                  gTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTa                                                                                                                   >  1:3056883/1‑150 (MQ=255)
                                    aaTATTTAATGAACCTGTGCCATAGCTAACTATTTCGAATAATTCGGTCGTCAGAAAAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAAAAGCCTCCATCGACGAGACCCTTCTGTTTAat                                                                                                                 >  2:4307797/1‑150 (MQ=255)
                                       atTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATAtt                                                                                                              <  2:1581273/150‑1 (MQ=255)
                                       atTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATAtt                                                                                                              >  1:1273192/1‑150 (MQ=255)
                                       atTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATAtt                                                                                                              >  1:1291706/1‑150 (MQ=255)
                                          tAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAg                                                                                                           >  2:205452/1‑150 (MQ=255)
                                             tGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGt                                                                                                        <  2:3325627/150‑1 (MQ=255)
                                                aCCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATAGACGTGACCCTTCTGTTTAATATTCAGCGTccc                                                                                                     >  1:426529/1‑150 (MQ=255)
                                                 ccTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTcccc                                                                                                    >  2:1189855/1‑150 (MQ=255)
                                                   tgtgCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTAAGCGTCCCCGt                                                                                                  >  1:2764433/1‑150 (MQ=255)
                                                      gCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTcc                                                                                               >  1:2657317/1‑150 (MQ=255)
                                                       ccATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCg                                                                                              >  2:2500063/1‑150 (MQ=255)
                                                          tAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGAt                                                                                           >  1:2906222/1‑150 (MQ=255)
                                                           aGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGAtt                                                                                          <  2:637763/150‑1 (MQ=255)
                                                            gCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTg                                                                                         >  2:4340900/1‑150 (MQ=255)
                                                            gCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTg                                                                                         >  1:4487212/1‑150 (MQ=255)
                                                             cTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGa                                                                                        >  1:2047393/1‑150 (MQ=255)
                                                                ccTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCTACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGAccc                                                                                     >  2:1225135/1‑150 (MQ=255)
                                                                 cTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCca                                                                                    >  1:100786/1‑150 (MQ=255)
                                                                       cGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTa                                                                              >  1:2752005/1‑150 (MQ=255)
                                                                        gAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTaa                                                                             >  1:4359504/1‑150 (MQ=255)
                                                                         aataatTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTaaa                                                                            <  1:2717535/150‑1 (MQ=255)
                                                                             atTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGcc                                                                        >  2:1187249/1‑150 (MQ=255)
                                                                                 ggTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGc                                                                    >  2:4356315/1‑150 (MQ=255)
                                                                                  gtcgtcAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCa                                                                   <  1:613801/150‑1 (MQ=255)
                                                                                   tcgtcAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCat                                                                  >  2:3615409/1‑150 (MQ=255)
                                                                                    cgtcAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCatt                                                                 >  2:579516/1‑150 (MQ=255)
                                                                                       cAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCattatt                                                              >  1:2305429/1‑150 (MQ=255)
                                                                                       cAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCattatt                                                              >  1:807038/1‑150 (MQ=255)
                                                                                        aGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTAttt                                                             >  2:278125/1‑150 (MQ=255)
                                                                                        aGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTAttt                                                             >  2:1305049/1‑150 (MQ=255)
                                                                                          aaCAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTcc                                                           <  2:1088111/150‑1 (MQ=255)
                                                                                             agagTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCt                                                        <  2:4117651/150‑1 (MQ=255)
                                                                                             agagTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATATAAAGGCCTTGCATTATTTACGCt                                                        >  1:1367413/1‑150 (MQ=255)
                                                                                               agTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTAt                                                      <  2:1983870/150‑1 (MQ=255)
                                                                                                gTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATc                                                     >  2:4273965/1‑150 (MQ=255)
                                                                                                gTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATc                                                     <  2:1406934/150‑1 (MQ=255)
                                                                                                 tCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCa                                                    <  1:367174/150‑1 (MQ=255)
                                                                                                 tCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCa                                                    <  1:1097074/150‑1 (MQ=255)
                                                                                                  ccTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCAt                                                   >  2:2450225/1‑150 (MQ=255)
                                                                                                  ccTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCAt                                                   <  2:3488915/150‑1 (MQ=255)
                                                                                                   ctctCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATa                                                  <  2:3440111/150‑1 (MQ=255)
                                                                                                    tctcCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGTAACTAATCTTTAATAGCCTCCATCGACGTGTCCCTTCTGTTTAATATTCAGCTTCCCCGTTCCGTATTGACCCAAATTTAAAGGCCTTGCATTATTTGCGCTATCTTat                                                 <  2:1768918/150‑2 (MQ=255)
                                                                                                    tctcCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATAc                                                 <  1:1396884/150‑1 (MQ=255)
                                                                                                    tctcCCTCAACATTGACCGTCCCGACGCCTACTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATAc                                                 <  1:3961415/150‑1 (MQ=255)
                                                                                                     ctcCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACa                                                <  2:329248/150‑1 (MQ=255)
                                                                                                      tcCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACaa                                               >  2:1625446/1‑150 (MQ=255)
                                                                                                        ccTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATc                                             <  2:1293852/150‑1 (MQ=255)
                                                                                                        ccTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATc                                             >  1:56912/1‑150 (MQ=255)
                                                                                                           cAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGcc                                          <  1:2567746/150‑1 (MQ=255)
                                                                                                            aaCATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCa                                         >  1:4046052/1‑150 (MQ=255)
                                                                                                            aaCATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCa                                         >  1:2937130/1‑150 (MQ=255)
                                                                                                            aaCATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCa                                         >  1:4005393/1‑150 (MQ=255)
                                                                                                            aaCATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCa                                         <  2:3823374/150‑1 (MQ=255)
                                                                                                             aCATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCCTCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCGTGCATTATTTCCGCTATCATACAATCGCCAg                                        <  2:1210915/150‑1 (MQ=255)
                                                                                                              cATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAgg                                       <  1:1990534/150‑1 (MQ=255)
                                                                                                                   aCCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTAAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCa                                  <  2:2721737/150‑1 (MQ=255)
                                                                                                                     cGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTGGCATTATTTCCGCTATCATACAATCGCCAGGTGCCAcc                                <  1:159131/150‑1 (MQ=255)
                                                                                                                        cccGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCaa                             <  1:2432068/150‑1 (MQ=255)
                                                                                                                         ccGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCaaa                            >  2:1920741/1‑150 (MQ=255)
                                                                                                                           gACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAAc                          <  2:1307065/150‑1 (MQ=255)
                                                                                                                            aCGCCTCCTGTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACa                         <  1:923780/150‑1 (MQ=255)
                                                                                                                                ctcctGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTaa                     >  1:2239927/1‑150 (MQ=255)
                                                                                                                                   ctGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACgg                  >  1:3540737/1‑150 (MQ=255)
                                                                                                                                    tGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGt                 >  1:2170886/1‑150 (MQ=255)
                                                                                                                                     gTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTg                <  2:2641130/150‑1 (MQ=255)
                                                                                                                                     gTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTg                >  2:2569016/1‑150 (MQ=255)
                                                                                                                                     gTCGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTg                <  2:3569686/150‑1 (MQ=255)
                                                                                                                                      tCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGc               <  2:2879503/150‑1 (MQ=255)
                                                                                                                                       cGAAGAACCTAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGcc              <  1:1771438/150‑1 (MQ=255)
                                                                                                                                         aagaaCCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCct            <  1:4518885/150‑1 (MQ=255)
                                                                                                                                          agaaCCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCctc           <  2:3998971/150‑1 (MQ=255)
                                                                                                                                          agaaCCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCctc           >  1:3001562/1‑150 (MQ=255)
                                                                                                                                          agaaCCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCctc           >  2:217624/1‑150 (MQ=255)
                                                                                                                                          agaaCCTAATCTTAAATAGCCTCCATAGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCctc           <  1:4429282/150‑1 (MQ=255)
                                                                                                                                           gaaCCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCctct          <  1:1207387/150‑1 (MQ=255)
                                                                                                                                                tAATATTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTGGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTc     <  1:708261/150‑1 (MQ=255)
                                                                                                                                                 aaTCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTcc    >  1:2620691/1‑150 (MQ=255)
                                                                                                                                                   tCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTc  <  2:1522887/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGCGACGATTGAACTCGTGACGTAACCCTTATCCGTAATATTTAATGAACCTGTGCCATAGCTACCTATTTCGAATAATTCGGTCGTCAGAACAGAGTCCTCTCCCTCAACATTGACCGTCCCGACGCCTCCTGTCGAAGAACCTAATCTTAAATAGCCTCCATCGACGTGACCCTTCTGTTTAATATTCAGCGTCCCCGTTCCGGATTGACCCACATTTAAAGGCCTTGCATTATTTCCGCTATCATACAATCGCCAGGTGCCACCCAAAACATTAACGGTGCCCTCTGAGTCCTC  >  CP009273/1591705‑1592001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑