BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,610,766	C→T	59.2%	S249S (AGC→AGT)	yneK →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,610,766	0	C	T	59.2%	161.6 / inf	272	S249S (AGC→AGT)	yneK	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (46/65); new base T (70/91); total (116/156)
Fisher's exact test for biased strand distribution p-value = 8.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 272 total aligned reads displayed.

TTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTAT  >  CP009273/1610619‑1610912

 tttATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTg                                                                                                                                                 <  2:2562564/150‑1 (MQ=255)

  ttATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGa                                                                                                                                                >  1:794375/1‑150 (MQ=255)

        ctcGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGAtt                                                                                                                                          <  2:797016/150‑1 (MQ=255)

        ctcGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGAtt                                                                                                                                          >  2:1261578/1‑150 (MQ=255)

            cgGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCAtt                                                                                                                                      >  1:103941/1‑150 (MQ=255)

                aaTTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGt                                                                                                                                  <  2:1276352/150‑1 (MQ=255)

                aaTTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGt                                                                                                                                  <  2:1894300/150‑1 (MQ=255)

                 aTTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGtt                                                                                                                                 >  2:3473027/1‑150 (MQ=255)

                    ttGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATAAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTa                                                                                                                              >  1:4389926/1‑150 (MQ=255)

                     tGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTaa                                                                                                                             >  1:2888438/1‑150 (MQ=255)

                     tGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTaa                                                                                                                             <  2:2787535/150‑1 (MQ=255)

                      gTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTaac                                                                                                                            >  2:4482030/1‑150 (MQ=255)

                            cTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACAt                                                                                                                      >  1:1034868/1‑150 (MQ=255)

                                   gATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGtactac                                                                                                               >  1:2331870/1‑150 (MQ=255)

                                     ttATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTAccc                                                                                                             <  1:1658584/150‑1 (MQ=255)

                                        tGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGaa                                                                                                          >  2:286659/1‑150 (MQ=255)

                                        tGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGaa                                                                                                          >  1:2047848/1‑150 (MQ=255)

                                              aaCTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGc                                                                                                    >  2:4362681/1‑150 (MQ=255)

                                               aCTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCa                                                                                                   >  2:630834/1‑150 (MQ=255)

                                                ctctGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCAt                                                                                                  >  1:1337809/1‑150 (MQ=255)

                                                 tctGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATc                                                                                                 >  1:3316960/1‑150 (MQ=255)

                                                   tGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTg                                                                                               >  2:4026862/1‑150 (MQ=255)

                                                   tGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTg                                                                                               >  1:4048236/1‑150 (MQ=255)

                                                    gAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGc                                                                                              <  2:2106561/150‑1 (MQ=255)

                                                    gAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGc                                                                                              <  2:1442834/150‑1 (MQ=255)

                                                     aGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCt                                                                                             <  2:891022/150‑1 (MQ=255)

                                                      ggTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCtt                                                                                            <  2:3626617/150‑1 (MQ=255)

                                                      ggTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCtt                                                                                            <  2:3452674/150‑1 (MQ=255)

                                                        ttCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCtttt                                                                                          <  1:4298105/150‑1 (MQ=255)

                                                         tCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCttttt                                                                                         <  2:2129712/150‑1 (MQ=255)

                                                           acaGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTAt                                                                                       >  1:3951748/1‑150 (MQ=255)

                                                             aGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATcc                                                                                     <  1:3927805/150‑1 (MQ=255)

                                                             aGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATcc                                                                                     >  1:3842940/1‑150 (MQ=255)

                                                              gATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATccc                                                                                    <  2:480254/150‑1 (MQ=255)

                                                                tGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCtt                                                                                  <  2:3336460/150‑1 (MQ=255)

                                                                tGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCtt                                                                                  <  1:2243538/150‑1 (MQ=255)

                                                                 ggTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCttt                                                                                 >  1:1456651/1‑150 (MQ=255)

                                                                 ggTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCttt                                                                                 <  2:1120394/150‑1 (MQ=255)

                                                                 ggTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCttt                                                                                 <  1:2510306/150‑1 (MQ=255)

                                                                   ttCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTa                                                                               <  1:2059238/150‑1 (MQ=255)

                                                                     cGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGc                                                                             <  1:3095868/150‑1 (MQ=255)

                                                                      gTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCa                                                                            <  2:1603876/150‑1 (MQ=255)

                                                                       tCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAg                                                                           <  1:2613624/150‑1 (MQ=255)

                                                                        cAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGa                                                                          >  2:1318811/1‑150 (MQ=255)

                                                                         aaTAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGaa                                                                         >  1:2289213/1‑150 (MQ=255)

                                                                            aaaGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACaa                                                                      >  1:2625659/1‑150 (MQ=255)

                                                                             aaGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAg                                                                     <  1:3193911/150‑1 (MQ=255)

                                                                              aGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGa                                                                    <  1:3773922/150‑1 (MQ=255)

                                                                               gATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGaa                                                                   <  2:1743909/150‑1 (MQ=255)

                                                                                 tCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAAtc                                                                 <  1:1113886/150‑1 (MQ=255)

                                                                                   cTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAAtctc                                                               <  2:4459790/150‑1 (MQ=255)

                                                                                   cTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAAtctc                                                               <  1:2777854/150‑1 (MQ=255)

                                                                                   cTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAAtctc                                                               <  1:3130115/150‑1 (MQ=255)

                                                                                    tAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATctcc                                                              >  2:236427/1‑150 (MQ=255)

                                                                                    tAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATctcc                                                              <  2:1535422/150‑1 (MQ=255)

                                                                                     aaCTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATctcct                                                             >  2:3057694/1‑150 (MQ=255)

                                                                                     aaCTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATctcct                                                             >  2:3720013/1‑150 (MQ=255)

                                                                                       cTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCa                                                           <  2:243107/150‑1 (MQ=255)

                                                                                       cTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCa                                                           <  2:2579475/150‑1 (MQ=255)

                                                                                         tGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCAtt                                                         >  1:3636639/1‑150 (MQ=255)

                                                                                         tGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCAtt                                                         >  1:296578/1‑150 (MQ=255)

                                                                                          gATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCAttt                                                        >  1:506294/1‑150 (MQ=255)

                                                                                          gATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCAttt                                                        >  1:2094425/1‑150 (MQ=255)

                                                                                          gATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCAttt                                                        >  1:2824569/1‑150 (MQ=255)

                                                                                          gATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCAttt                                                        <  2:2876767/150‑1 (MQ=255)

                                                                                           aTCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCAtttt                                                       <  1:806491/150‑1 (MQ=255)

                                                                                                 gCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAAc                                                 <  1:121127/150‑1 (MQ=255)

                                                                                                 gCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAAc                                                 <  2:3817660/150‑1 (MQ=255)

                                                                                                 gCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAAc                                                 >  1:1040108/1‑150 (MQ=255)

                                                                                                  ccATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACt                                                <  2:1508739/150‑1 (MQ=255)

                                                                                                   cATTCTCGACATTACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACtt                                               <  2:399219/150‑1 (MQ=255)

                                                                                                    aTTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAATCATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTg                                              <  2:1865487/150‑1 (MQ=255)

                                                                                                     ttCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGt                                             <  2:2703094/150‑1 (MQ=255)

                                                                                                     ttCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGt                                             >  1:855664/1‑150 (MQ=255)

                                                                                                       ctcGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGttt                                           >  2:1009618/1‑150 (MQ=255)

                                                                                                       ctcGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGttt                                           >  2:167359/1‑150 (MQ=255)

                                                                                                         cGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGttttt                                         >  2:3129158/1‑150 (MQ=255)

                                                                                                          gACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTa                                        >  2:3859361/1‑150 (MQ=255)

                                                                                                             aTAACGGACAGCGAATTAAAAGAGTATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTAttt                                     <  2:3906356/150‑1 (MQ=255)

                                                                                                             aTAACGGACAGCGAATTAAAAGAGGATTTGTTGCAATGTCTGATGGAGTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTGTAttt                                     >  2:4282633/1‑150 (MQ=255)

                                                                                                              tAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTAtttt                                    <  1:942848/150‑1 (MQ=255)

                                                                                                                aCGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTc                                  <  2:4511372/150‑1 (MQ=255)

                                                                                                                   gACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGcc                               >  1:680479/1‑150 (MQ=255)

                                                                                                                     cAGCGAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCaa                             >  2:1932453/1‑150 (MQ=255)

                                                                                                                       gCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAata                           <  1:1942700/150‑1 (MQ=255)

                                                                                                                         gAATTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATg                         <  1:4224210/150‑1 (MQ=255)

                                                                                                                         gAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATg                         <  2:4140941/150‑1 (MQ=255)

                                                                                                                          aaTTAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGt                        >  2:843801/1‑150 (MQ=255)

                                                                                                                          aaTTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGt                        <  1:3201039/150‑1 (MQ=255)

                                                                                                                           aTTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGtt                       >  1:1359825/1‑150 (MQ=255)

                                                                                                                             tAAAAGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTAt                     >  1:3430524/1‑150 (MQ=255)

                                                                                                                              aaaaGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTAtt                    <  1:917745/150‑1 (MQ=255)

                                                                                                                                aaGAGGATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGc                  >  2:3420073/1‑150 (MQ=255)

                                                                                                                                     gATTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGACCAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTAt             >  2:2494218/1‑150 (MQ=255)

                                                                                                                                      aTTTGTTGCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATc            <  2:756329/150‑1 (MQ=255)

                                                                                                                                            tgCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAAt      <  1:4575019/150‑1 (MQ=255)

                                                                                                                                             gCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGTACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAAtt     <  2:2959468/150‑1 (MQ=255)

                                                                                                                                             gCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAAtt     >  2:4143442/1‑150 (MQ=255)

                                                                                                                                             gCAAAGTCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAAtt     <  2:1637770/150‑1 (MQ=255)

                                                                                                                                                aaGCCTGATGGATTCATTAGGTTCTAAAAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTAt  <  1:3968194/150‑1 (MQ=255)

TTTTATCTCTCGCGGTAATTTTGTCATACTCCCCCGATTATGGAATAACTCTGAGGTTCACAGATGGTTCGTCAATAAAGATCCTAACTTGATCACCGCCATTCTCGACATAACGGACAGCGAATTAAAAGAGGATTTGTTGCAAAGCCTGATGGATTCATTAGGTTCTAACAAACATGTACTACCCGAAGTCTGCATCTGCTTTTTATCCCTTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTAT  >  CP009273/1610619‑1610912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`