Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,616,682 T→G 11.0% L365R (CTG→CGG)  ydeE → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,616,6820TG11.0% 771.1 / 101.7 300L365R (CTG→CGG) ydeEputative transporter
Reads supporting (aligned to +/- strand):  ref base T (147/120);  new base G (19/14);  total (166/134)
Fisher's exact test for biased strand distribution p-value = 8.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01

Only 100 of 303 total aligned reads displayed.

TGCGCCGGGCGAGTATATGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGTTACCTCTGCT  >  CP009273/1616533‑1616831
                                                                                                                                                     |                                                                                                                                                     
    ccGGGCGAGTATATGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGttt                                                                                                                                                   >  1:3223611/1‑150 (MQ=255)
     cGGGCGAGTATATGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTg                                                                                                                                                  >  1:2808743/1‑150 (MQ=255)
     cGGGCGAGTATATGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCGGTTTg                                                                                                                                                  <  1:4175709/150‑1 (MQ=255)
       ggCGAGTATATGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTc                                                                                                                                                >  2:2935947/1‑150 (MQ=255)
       ggCGAGTATATGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTc                                                                                                                                                >  1:4426267/1‑150 (MQ=255)
         cGAGTATATGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCAt                                                                                                                                              <  2:1072841/150‑1 (MQ=255)
         cGAGTATATGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCAt                                                                                                                                              >  1:1920986/1‑150 (MQ=255)
          gAGTATATGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATc                                                                                                                                             >  2:2988282/1‑150 (MQ=255)
          gAGTATATGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATc                                                                                                                                             >  2:4248037/1‑150 (MQ=255)
          gAGTATATGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCGGTTTGTCATc                                                                                                                                             >  2:2902768/1‑150 (MQ=255)
           aGTATATGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCt                                                                                                                                            <  1:4317789/150‑1 (MQ=255)
              atatGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAg                                                                                                                                         <  2:676698/150‑1 (MQ=255)
              atatGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAg                                                                                                                                         >  1:2986556/1‑150 (MQ=255)
              atatGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCGGTTTGTCATCTTAg                                                                                                                                         <  2:1429902/150‑1 (MQ=255)
               tatGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGc                                                                                                                                        >  2:213586/1‑150 (MQ=255)
                atGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCg                                                                                                                                       <  2:3085085/150‑1 (MQ=255)
                    tgattgaCCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTgg                                                                                                                                   >  1:570617/1‑150 (MQ=255)
                     gattgaCCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGt                                                                                                                                  <  2:13336/150‑1 (MQ=255)
                     gattgaCCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGt                                                                                                                                  <  1:1874975/150‑1 (MQ=255)
                      attgaCCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGACGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTg                                                                                                                                 <  2:4376685/150‑1 (MQ=255)
                       ttgaCCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGa                                                                                                                                >  1:1094099/1‑150 (MQ=255)
                        tgaCCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGAt                                                                                                                               >  2:4244516/1‑150 (MQ=255)
                        tgaCCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCGGTTTGTCATCTTAGCGTTGGTGAt                                                                                                                               >  1:582168/1‑150 (MQ=255)
                        tgaCCATAGTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGAt                                                                                                                               <  2:3122653/150‑1 (MQ=255)
                         gaCCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATc                                                                                                                              <  1:2657952/150‑1 (MQ=255)
                           ccATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCAt                                                                                                                            >  1:1179948/1‑150 (MQ=255)
                           ccATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCAt                                                                                                                            <  2:4287229/150‑1 (MQ=255)
                             atatTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATtg                                                                                                                          <  2:157222/150‑1 (MQ=255)
                               atTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATtgct                                                                                                                        <  1:3410040/150‑1 (MQ=255)
                               atTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCGGTTTGTCATCTTAGCGTTGGTGATCATtgct                                                                                                                        >  1:328397/1‑150 (MQ=255)
                                   cgccgcCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGt                                                                                                                    <  2:1214657/150‑1 (MQ=255)
                                    gccgccAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTg                                                                                                                   <  1:1568638/150‑1 (MQ=255)
                                          aGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCGGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCt                                                                                                             >  1:1485447/1‑150 (MQ=255)
                                            aaaTGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGa                                                                                                           >  2:4169306/1‑150 (MQ=255)
                                            aaaTGAAAGCCAGCTA‑TTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCGGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGAt                                                                                                          <  1:2846653/150‑1 (MQ=255)
                                             aaTGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGAt                                                                                                          >  2:1905965/1‑150 (MQ=255)
                                              aTGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATg                                                                                                         <  2:2546627/150‑1 (MQ=255)
                                                gAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGtt                                                                                                       >  1:3256479/1‑150 (MQ=255)
                                                   aGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCGGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTaaa                                                                                                    <  2:4516212/150‑1 (MQ=255)
                                                    gCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTaaaa                                                                                                   <  2:174957/150‑1 (MQ=255)
                                                     ccAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAg                                                                                                  >  2:2888930/1‑150 (MQ=255)
                                                     ccAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCGGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAg                                                                                                  >  1:2296973/1‑150 (MQ=255)
                                                       aGCTATTTTTCCGCCCAGTCTTTAGGCTGGNTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAggg                                                                                                >  1:4279828/1‑150 (MQ=255)
                                                        gCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGa                                                                                               >  1:1816317/1‑150 (MQ=255)
                                                         cTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGAt                                                                                              <  2:2642910/150‑1 (MQ=255)
                                                         cTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGAt                                                                                              <  1:3129570/150‑1 (MQ=255)
                                                           aTTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTc                                                                                            >  1:1365342/1‑150 (MQ=255)
                                                           aTTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTc                                                                                            <  1:4633610/150‑1 (MQ=255)
                                                           aTTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTc                                                                                            <  1:2929348/150‑1 (MQ=255)
                                                                tCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCa                                                                                       >  1:2614097/1‑150 (MQ=255)
                                                                   gCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGa                                                                                    >  2:4054307/1‑150 (MQ=255)
                                                                    cccAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGAc                                                                                   >  1:1330358/1‑150 (MQ=255)
                                                                     ccAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGAcc                                                                                  <  2:3335206/150‑1 (MQ=255)
                                                                             tAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGc                                                                          >  1:909897/1‑150 (MQ=255)
                                                                              aGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCa                                                                         <  1:978263/150‑1 (MQ=255)
                                                                                  tggctTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGccc                                                                     <  1:2538237/150‑1 (MQ=255)
                                                                                   ggctTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCcg                                                                    <  1:3317605/150‑1 (MQ=255)
                                                                                   ggctTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCcg                                                                    <  1:3190195/150‑1 (MQ=255)
                                                                                    gctTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCcgc                                                                   <  1:2945356/150‑1 (MQ=255)
                                                                                     ctTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCcgcg                                                                  >  1:1632644/1‑150 (MQ=255)
                                                                                          tGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTttg                                                             >  2:4583477/1‑150 (MQ=255)
                                                                                           gCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTttgt                                                            <  1:491763/150‑1 (MQ=255)
                                                                                           gCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCGGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTttgt                                                            >  2:2492866/1‑150 (MQ=255)
                                                                                            ccGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTttgtt                                                           >  1:2162040/1‑150 (MQ=255)
                                                                                               cgATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGAt                                                        <  1:3510423/150‑1 (MQ=255)
                                                                                                 aTTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGAttt                                                      >  2:4423080/1‑150 (MQ=255)
                                                                                                 aTTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGAttt                                                      >  1:3837598/1‑150 (MQ=255)
                                                                                                  ttAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTa                                                     >  2:3973808/1‑150 (MQ=255)
                                                                                                   tAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTaa                                                    >  1:870047/1‑150 (MQ=255)
                                                                                                   tAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTaa                                                    >  2:1525601/1‑150 (MQ=255)
                                                                                                     aCCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGt                                                  >  2:2866012/1‑150 (MQ=255)
                                                                                                      cccATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTc                                                 <  1:2275024/150‑1 (MQ=255)
                                                                                                       ccATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCg                                                >  1:3974674/1‑150 (MQ=255)
                                                                                                       ccATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCg                                                >  2:380057/1‑150 (MQ=255)
                                                                                                         aTTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGaa                                              <  1:2446190/150‑1 (MQ=255)
                                                                                                            agtgagtgGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAacac                                           >  2:3731555/1‑150 (MQ=255)
                                                                                                             gtgagtgGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAAcaca                                          <  2:3216369/150‑1 (MQ=255)
                                                                                                               gagtgGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAAt                                        <  2:893226/150‑1 (MQ=255)
                                                                                                                agtgGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATa                                       >  2:2818482/1‑150 (MQ=255)
                                                                                                                     cGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGAt                                  <  2:3626602/150‑1 (MQ=255)
                                                                                                                     cGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGAt                                  >  2:2057620/1‑150 (MQ=255)
                                                                                                                        aGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTa                               >  2:484320/1‑150 (MQ=255)
                                                                                                                         gTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTaa                              >  2:3647142/1‑150 (MQ=255)
                                                                                                                          tGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAAt                             <  1:2890048/150‑1 (MQ=255)
                                                                                                                           gCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAAtt                            <  2:2230953/150‑1 (MQ=255)
                                                                                                                            cTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTc                           <  1:4054307/150‑1 (MQ=255)
                                                                                                                             tAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCa                          <  1:762530/150‑1 (MQ=255)
                                                                                                                               aCCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCAGGGCTGCGTGGGTGCTGATGTTAAAAGTGATTCGAGCAAGTCCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGc                        <  1:2118767/150‑1 (MQ=255)
                                                                                                                                ccAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGcc                       <  1:767791/150‑1 (MQ=255)
                                                                                                                                        ccgccTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTa               <  2:2412910/150‑1 (MQ=255)
                                                                                                                                          gccTTCCTCGCGGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAgg             >  1:3645988/1‑150 (MQ=255)
                                                                                                                                           ccTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGt            >  2:3534784/1‑150 (MQ=255)
                                                                                                                                            cTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGtt           >  2:2823546/1‑150 (MQ=255)
                                                                                                                                            cTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGtt           >  1:2556388/1‑150 (MQ=255)
                                                                                                                                                cTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGTTACct       >  1:4438249/1‑150 (MQ=255)
                                                                                                                                                 tCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGTTACctc      <  1:459798/150‑1 (MQ=255)
                                                                                                                                                  cGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGTTACctct     >  1:1949256/1‑150 (MQ=255)
                                                                                                                                                  cGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGTTACctct     >  1:660965/1‑150 (MQ=255)
                                                                                                                                                  cGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGTTACctct     >  2:3878033/1‑150 (MQ=255)
                                                                                                                                                     tGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGTTACCTCTGCt  <  1:275243/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGCGCCGGGCGAGTATATGTTGATTGACCATATTGCGCCGCCAGAAATGAAAGCCAGCTATTTTTCCGCCCAGTCTTTAGGCTGGCTTGGTGCCGCGATTAACCCATTAGTGAGTGGCGTAGTGCTAACCAGCCTGCCGCCTTCCTCGCTGTTTGTCATCTTAGCGTTGGTGATCATTGCTGCGTGGGTGCTGATGTTAAAAGGGATTCGAGCAAGACCGTGGGGGCAGCCCGCGCTTTGTTGATTTAAGTCGAACACAATAAAGATTTAATTCAGCCTTCGTTTAGGTTACCTCTGCT  >  CP009273/1616533‑1616831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑