Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,701,260 T→A 57.4% S29T (TCC→ACC)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,701,2600TA57.4% 157.4 / inf 275S29T (TCC→ACC) rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base T (68/49);  new base A (93/65);  total (161/114)
Fisher's exact test for biased strand distribution p-value = 9.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 278 total aligned reads displayed.

AACACCTGACTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCA  >  CP009273/1701111‑1701404
                                                                                                                                                     |                                                                                                                                                
aaCACCTGACTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGt                                                                                                                                                  >  2:4049892/1‑150 (MQ=255)
    ccTGACTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCaa                                                                                                                                              <  2:4643689/150‑1 (MQ=255)
      tGACTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACg                                                                                                                                            <  1:4609925/150‑1 (MQ=255)
      tGACTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACg                                                                                                                                            >  2:1864453/1‑150 (MQ=255)
        aCTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGt                                                                                                                                          <  1:713326/150‑1 (MQ=255)
                   tGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTgcg                                                                                                                               <  1:3647834/150‑1 (MQ=255)
                   tGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTgcg                                                                                                                               >  2:1676993/1‑150 (MQ=255)
                     ggATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGcc                                                                                                                             >  2:601510/1‑150 (MQ=255)
                      gATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCa                                                                                                                            >  1:4549367/1‑150 (MQ=255)
                          tGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTa                                                                                                                        >  2:3877986/1‑150 (MQ=255)
                          tGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTa                                                                                                                        >  2:44073/1‑150 (MQ=255)
                            aaCACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTAcc                                                                                                                      <  2:1244407/150‑1 (MQ=255)
                              cacCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAcccc                                                                                                                    <  1:4392643/150‑1 (MQ=255)
                               acCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCt                                                                                                                   <  1:887023/150‑1 (MQ=255)
                                ccATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTg                                                                                                                  <  2:3336107/150‑1 (MQ=255)
                                ccATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTg                                                                                                                  >  1:3172319/1‑150 (MQ=255)
                                 cATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTgg                                                                                                                 >  1:2592841/1‑150 (MQ=255)
                                  aTTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGgc                                                                                                                <  2:4013640/150‑1 (MQ=255)
                                  aTTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGgc                                                                                                                >  2:1952886/1‑150 (MQ=255)
                                     cccGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCa                                                                                                             >  2:2494762/1‑150 (MQ=255)
                                       cGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGTATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGc                                                                                                           >  1:3033983/1‑150 (MQ=255)
                                        gTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCg                                                                                                          >  2:3833841/1‑150 (MQ=255)
                                           cGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGttt                                                                                                       <  1:512952/150‑1 (MQ=255)
                                                  ttCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTAtt                                                                                                <  2:728371/150‑1 (MQ=255)
                                                   tCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTc                                                                                               >  2:2678514/1‑150 (MQ=255)
                                                        tGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTg                                                                                          >  1:529393/1‑150 (MQ=255)
                                                          gAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGaa                                                                                        <  2:3875707/150‑1 (MQ=255)
                                                                    cTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATAtt                                                                              >  1:4188392/1‑150 (MQ=255)
                                                                    cTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATAtt                                                                              >  2:3529259/1‑150 (MQ=255)
                                                                    attAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATAtt                                                                              <  1:1225845/149‑1 (MQ=255)
                                                                     ttAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTg                                                                             <  1:835138/150‑1 (MQ=255)
                                                                       aaGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGgc                                                                           >  2:718986/1‑150 (MQ=255)
                                                                       aaGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGgc                                                                           >  1:2296094/1‑150 (MQ=255)
                                                                             ttCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGaa                                                                     >  1:3729725/1‑150 (MQ=255)
                                                                              tctcTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTACACTGAAACAGCATATTGGCGCTGAAg                                                                    >  1:466357/1‑150 (MQ=255)
                                                                                    cATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGt                                                              >  2:3932541/1‑150 (MQ=255)
                                                                                      ttCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTtg                                                            <  2:250977/150‑1 (MQ=255)
                                                                                       tCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTtgt                                                           <  2:768498/150‑1 (MQ=255)
                                                                                       tCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCAGATTGGCGCTGAAGGTGAGTtgt                                                           >  1:419049/1‑150 (MQ=255)
                                                                                         aGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGc                                                         <  1:4252316/150‑1 (MQ=255)
                                                                                             aaaataaaatCTGTGATTTCAACGGCTGCATCCATCCACCGGATATGAAAACCCAGACCAACGTTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAATGTGAGTTTTGCGTTa                                                     <  1:1011266/150‑1 (MQ=255)
                                                                                              aaataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAg                                                    >  2:822986/1‑150 (MQ=255)
                                                                                              aaataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAg                                                    >  2:1236803/1‑150 (MQ=255)
                                                                                                ataaaatCTGGGATTTCAACGGCGGCCTCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCg                                                  <  2:2334035/150‑1 (MQ=255)
                                                                                                  aaaatCTGGTATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTc                                                <  1:952906/150‑1 (MQ=255)
                                                                                                   aaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCg                                               >  2:3395194/1‑150 (MQ=255)
                                                                                                     atCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGc                                             >  1:2155454/1‑150 (MQ=255)
                                                                                                     atCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTGGTGCGTTAGCGTCGGc                                             >  1:3063882/1‑150 (MQ=255)
                                                                                                       cTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGcaa                                           >  2:4202037/1‑148 (MQ=255)
                                                                                                       cTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGa                                           >  2:2182753/1‑150 (MQ=255)
                                                                                                       cTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGa                                           >  2:1748141/1‑150 (MQ=255)
                                                                                                         gggATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATa                                         <  2:2854750/150‑1 (MQ=255)
                                                                                                           gATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATaaa                                       >  1:3719681/1‑150 (MQ=255)
                                                                                                           gATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATaaa                                       >  1:3360979/1‑150 (MQ=255)
                                                                                                            aTTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAg                                      <  2:3801493/150‑1 (MQ=255)
                                                                                                            aTTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAg                                      >  1:2629390/1‑150 (MQ=255)
                                                                                                            aTTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAg                                      <  2:694061/150‑1 (MQ=255)
                                                                                                            aTTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAg                                      >  1:1810129/1‑150 (MQ=255)
                                                                                                              ttCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTa                                    >  1:689944/1‑150 (MQ=255)
                                                                                                               tCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCCGGTACCCCGGGCGCAGCGGTTTGTTATTCCACTGAAACCGCAGATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTAt                                   >  2:3901402/1‑150 (MQ=255)
                                                                                                                cAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTAtt                                  <  2:499358/150‑1 (MQ=255)
                                                                                                                cAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTAtt                                  >  1:158113/1‑150 (MQ=255)
                                                                                                                 aaCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTg                                 >  2:2814256/1‑150 (MQ=255)
                                                                                                                 aaCGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTg                                 >  1:3293076/1‑150 (MQ=255)
                                                                                                                 aaCGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTg                                 >  2:4258194/1‑150 (MQ=255)
                                                                                                                 aaCGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTg                                 >  1:4534295/1‑150 (MQ=255)
                                                                                                                 aaCGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACGGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTg                                 >  1:1364772/1‑150 (MQ=255)
                                                                                                                  aCGGCGGCATCCATCCACCGGAGATTAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgc                                <  1:2138965/150‑1 (MQ=255)
                                                                                                                  aCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgc                                <  1:1144555/150‑1 (MQ=255)
                                                                                                                  aCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgc                                <  1:29521/150‑1 (MQ=255)
                                                                                                                  aCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgc                                <  2:1156709/150‑1 (MQ=255)
                                                                                                                  aCGGCGGCATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCCGGTACCCCTGGCGCAGCGTTTTGTTAATCCAATTAAACAGCATATTGGCGATGAAGGTGAGTTGTGCTGTAGCGTCGGCGATAAAGTATTgc                                >  1:2006140/1‑150 (MQ=255)
                                                                                                                       ggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGc                           >  1:1641003/1‑150 (MQ=255)
                                                                                                                       ggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGc                           >  2:470251/1‑150 (MQ=255)
                                                                                                                       ggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGc                           >  1:3572613/1‑150 (MQ=255)
                                                                                                                       ggcATCCATCCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGc                           >  2:2440834/1‑150 (MQ=255)
                                                                                                                         catccatcCACCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCa                         <  2:1432668/150‑1 (MQ=255)
                                                                                                                            ccatccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTTCGCGGCCAGcc                      >  1:3974760/1‑150 (MQ=255)
                                                                                                                            ccatccaCCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGcc                      >  2:3571965/1‑150 (MQ=255)
                                                                                                                            ccatccaCAGTAGATGAAAACACATACCAACGGAACACCCCTGCGCAAGGTACACCTGGCGCAGAGTTGTTATATTCCACTTAAACAGCATAAAGTCGCTTAAGTAGATTTGTGCGTTAGCGTCGGCGATAAAGTATTTCGCGGCCAGcc                      >  2:2240124/1‑150 (MQ=255)
                                                                                                                             catccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCg                     <  2:3118483/150‑1 (MQ=255)
                                                                                                                              atccaCCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGc                    <  1:2678514/150‑1 (MQ=255)
                                                                                                                               tccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCt                   <  2:4184882/150‑1 (MQ=255)
                                                                                                                               tccaCCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCt                   <  1:2095535/150‑1 (MQ=255)
                                                                                                                               tccaCCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCt                   <  2:4234893/150‑1 (MQ=255)
                                                                                                                                ccaCCGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCtt                  <  1:2360386/150‑1 (MQ=255)
                                                                                                                                   ccGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTAcc               <  2:1698451/150‑1 (MQ=255)
                                                                                                                                    cGGAGATGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTATCGTCGGCGATAAAGTATTTCGCCGCCAGCCGCTTAccc              >  1:2422981/1‑150 (MQ=255)
                                                                                                                                      gagaTGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGt            <  1:3414205/150‑1 (MQ=255)
                                                                                                                                      gagaTGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGt            <  2:1543543/150‑1 (MQ=255)
                                                                                                                                      gagaTGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATAGTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGt            >  2:889274/1‑150 (MQ=255)
                                                                                                                                      gagaTGAAAACCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTAGTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGt            <  2:3438823/150‑1 (MQ=255)
                                                                                                                                           gAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGTGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTcg       >  1:2340870/1‑150 (MQ=255)
                                                                                                                                           gAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTcg       <  2:4587795/150‑1 (MQ=255)
                                                                                                                                           gAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTcg       <  1:3833841/150‑1 (MQ=255)
                                                                                                                                           gAAAACCCAGACCAACGGTACACCCCTTCCCCCCGTACCCCTGGCGCAGCGTTTTTTTATTCCACTGAAACAACCTAAATGCGCTGAAGGTGAGTTGTGAGTTAGCGTCGGCGATAAAGTATAGCGCGGCCACCCGCTTAACCCTGGtag       >  1:2041962/1‑148 (MQ=255)
                                                                                                                                            aaaaCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTcgc      >  1:2527824/1‑150 (MQ=255)
                                                                                                                                            aaaaCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTcgc      <  1:1505248/150‑1 (MQ=255)
                                                                                                                                            aaaaCCCAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCCGCCGCTTACCCGTGGTcgc      >  2:1359581/1‑150 (MQ=255)
                                                                                                                                                cccAGACCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCCGCCGCTTACCCGTGGTCGCGGCa  >  2:3419848/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
AACACCTGACTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCA  >  CP009273/1701111‑1701404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑