Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,755,736 C→A 15.2% G105* (GGA→TGA)  sufD ← component of SufBCD Fe‑S cluster assembly scaffold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,755,7360CA15.2% 1014.6 / 109.1 310G105* (GGA→TGA) sufDcomponent of SufBCD Fe‑S cluster assembly scaffold
Reads supporting (aligned to +/- strand):  ref base C (126/137);  new base A (21/26);  total (147/163)
Fisher's exact test for biased strand distribution p-value = 7.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

Only 100 of 316 total aligned reads displayed.

TAACAGCAATGGCTTTGCCGGCCGTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTT  >  CP009273/1755587‑1755885
                                                                                                                                                     |                                                                                                                                                     
 aaCAGCAATGGCTTTGCCGGCCGTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCg                                                                                                                                                      >  2:1522822/1‑150 (MQ=255)
 aaCAGCAATGGCTTTGCCGGCCGTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCg                                                                                                                                                      >  2:1389776/1‑150 (MQ=255)
  aCAGCAATGGCTTTGCCGGCCGTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGc                                                                                                                                                     >  1:412610/1‑150 (MQ=255)
    aGCAATGGCTTTGCCGGCCGTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTg                                                                                                                                                   >  1:358629/1‑150 (MQ=255)
       aaTGGCTTTGCCGGCCGTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCAGCTGCCt                                                                                                                                                >  2:80066/1‑150 (MQ=255)
       aaTGGCTTTGCCGGCCGTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCAGCTGCCt                                                                                                                                                >  2:80420/1‑150 (MQ=255)
        aTGGCTTTGCCGGCCGTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGGTAATGCTCACTTCATATCCGCTGCCtt                                                                                                                                               >  2:3795695/1‑150 (MQ=255)
         tGGCTTTGCCGGCCGTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTc                                                                                                                                              <  1:752704/150‑1 (MQ=255)
            cTTTGCCGGCCGTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGt                                                                                                                                           >  1:2002576/1‑150 (MQ=255)
             tttGCCGGCCGTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGtt                                                                                                                                          >  1:4334405/1‑150 (MQ=255)
             tttGCCGGCCGTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGtt                                                                                                                                          >  2:3371925/1‑150 (MQ=255)
             tttGCCGGCCGTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGtt                                                                                                                                          >  1:1391988/1‑150 (MQ=255)
              ttGCCGGCCGTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTg                                                                                                                                         >  1:3889975/1‑150 (MQ=255)
                 ccggccgTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCAt                                                                                                                                      <  2:4450613/150‑1 (MQ=255)
                  cggccgTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATc                                                                                                                                     >  1:2981998/1‑150 (MQ=255)
                   ggccgTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCg                                                                                                                                    >  1:4025894/1‑150 (MQ=255)
                   ggccgTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCg                                                                                                                                    >  2:1298022/1‑150 (MQ=255)
                    gccgTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGc                                                                                                                                   >  1:4458365/1‑150 (MQ=255)
                     ccgTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCt                                                                                                                                  <  1:3534377/150‑1 (MQ=255)
                      cgTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTc                                                                                                                                 >  2:1159258/1‑150 (MQ=255)
                      cgTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTc                                                                                                                                 >  1:1012228/1‑150 (MQ=255)
                      cgTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTc                                                                                                                                 >  2:2425820/1‑150 (MQ=255)
                           aCCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCAGCTGCCTTCAGTTGCATCGCTCAGTGc                                                                                                                            >  1:1532739/1‑150 (MQ=255)
                                   tCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCAGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGt                                                                                                                    >  2:3150482/1‑150 (MQ=255)
                                    cACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTa                                                                                                                   >  1:2222591/1‑150 (MQ=255)
                                      cGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCAGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAAc                                                                                                                 <  1:3376790/150‑1 (MQ=255)
                                       ggCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACg                                                                                                                <  1:1240046/150‑1 (MQ=255)
                                       ggCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACg                                                                                                                <  2:2221170/150‑1 (MQ=255)
                                       ggCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACg                                                                                                                <  2:3560787/150‑1 (MQ=255)
                                           atatGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCa                                                                                                            >  2:3160507/1‑150 (MQ=255)
                                             atGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATc                                                                                                          <  1:2931592/150‑1 (MQ=255)
                                                  tCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACaa                                                                                                     >  1:609460/1‑150 (MQ=255)
                                                    aCGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCAGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAac                                                                                                   >  1:512961/1‑150 (MQ=255)
                                                       cTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCAGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACAcc                                                                                                <  2:3321192/150‑1 (MQ=255)
                                                          tgtgCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGc                                                                                             <  1:3324405/150‑1 (MQ=255)
                                                          tgtgCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGc                                                                                             <  1:4588670/150‑1 (MQ=255)
                                                           gtgCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGcc                                                                                            >  1:2223090/1‑150 (MQ=255)
                                                           gtgCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGcc                                                                                            <  1:1133005/150‑1 (MQ=255)
                                                           gtgCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCAGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGcc                                                                                            <  2:2025896/150‑1 (MQ=255)
                                                            tgCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCTTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGAACGTAACGCCCATCGACAAACACCAGcct                                                                                           <  2:1687997/150‑2 (MQ=255)
                                                            tgCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCg                                                                                           <  2:1010348/150‑1 (MQ=255)
                                                            tgCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCg                                                                                           <  2:42926/150‑1 (MQ=255)
                                                              ccAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCa                                                                                         <  2:181091/150‑1 (MQ=255)
                                                                 ggCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACgg                                                                                      <  2:1120147/150‑1 (MQ=255)
                                                                  gCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGa                                                                                     <  2:2743763/150‑1 (MQ=255)
                                                                  gCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGa                                                                                     <  1:3221894/150‑1 (MQ=255)
                                                                      tCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGTTAATCCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCTCTCAGTGCGGTCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCt                                                                                 <  2:2193381/150‑1 (MQ=255)
                                                                        cGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTaa                                                                               >  2:1183791/1‑150 (MQ=255)
                                                                           cAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGt                                                                            >  1:4113230/1‑150 (MQ=255)
                                                                           cAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGt                                                                            >  1:62525/1‑150 (MQ=255)
                                                                           cAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCAGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGt                                                                            <  2:4581102/150‑1 (MQ=255)
                                                                            aaaTGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGtt                                                                           >  2:3238019/1‑150 (MQ=255)
                                                                            aaaTGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCAGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGtt                                                                           >  1:2966215/1‑150 (MQ=255)
                                                                             aaTGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTa                                                                          <  2:4464599/150‑1 (MQ=255)
                                                                               tGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAAc                                                                        <  1:723463/150‑1 (MQ=255)
                                                                                      acacTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAgg                                                                 <  1:3700652/150‑1 (MQ=255)
                                                                                        acTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCa                                                               >  1:1718188/1‑150 (MQ=255)
                                                                                         cTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCAt                                                              <  2:2492711/150‑1 (MQ=255)
                                                                                          ttCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATc                                                             >  2:3859392/1‑150 (MQ=255)
                                                                                           tCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCa                                                            >  2:2635058/1‑150 (MQ=255)
                                                                                            ccGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCAc                                                           <  2:194585/150‑1 (MQ=255)
                                                                                             cGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                          >  1:1323892/1‑150 (MQ=255)
                                                                                                cTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctg                                                       <  2:2799362/150‑1 (MQ=255)
                                                                                                  gAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctgct                                                     >  1:82932/1‑150 (MQ=255)
                                                                                                   aaTAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGctgctg                                                    <  2:1214042/150‑1 (MQ=255)
                                                                                                   aaTAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGctgctg                                                    >  2:2116314/1‑150 (MQ=255)
                                                                                                    aTAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCtgt                                                   <  2:1623263/150‑1 (MQ=255)
                                                                                                      aGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTgg                                                 <  2:3719736/150‑1 (MQ=255)
                                                                                                      aGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTgg                                                 <  2:3476099/150‑1 (MQ=255)
                                                                                                      aGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACGTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTgg                                                 >  1:3138423/1‑150 (MQ=255)
                                                                                                         gTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCAGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGa                                              <  2:3919521/150‑1 (MQ=255)
                                                                                                            gggTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCAGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGatat                                           <  2:3835591/150‑1 (MQ=255)
                                                                                                             ggTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATAtc                                          <  2:1094742/150‑1 (MQ=255)
                                                                                                              gTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATAtct                                         <  2:1776821/150‑1 (MQ=255)
                                                                                                               tAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATAtctc                                        <  2:563823/150‑1 (MQ=255)
                                                                                                                aaaCCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATAtctct                                       >  1:960874/1‑150 (MQ=255)
                                                                                                                aaaCCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATAtctct                                       >  2:3291531/1‑150 (MQ=255)
                                                                                                                aaaCCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATAtctct                                       >  1:4245915/1‑150 (MQ=255)
                                                                                                                  aCCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTcc                                     >  1:1546752/1‑150 (MQ=255)
                                                                                                                    ccTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCg                                   <  2:2669456/150‑1 (MQ=255)
                                                                                                                      tGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCa                                 <  2:3022051/150‑1 (MQ=255)
                                                                                                                      tGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCa                                 <  1:910074/150‑1 (MQ=255)
                                                                                                                        aCGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAAt                               >  2:931965/1‑150 (MQ=255)
                                                                                                                        aCGGTCGTCGTTAATGCTCACTTCATATCAGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAAt                               <  2:2967455/150‑1 (MQ=255)
                                                                                                                             cgtcgtTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGa                          >  2:515519/1‑150 (MQ=255)
                                                                                                                              gtcgtTAATGCTCACTTCATATCAGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGAc                         <  1:3791547/150‑1 (MQ=255)
                                                                                                                                 gtTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa                      >  2:708979/1‑150 (MQ=255)
                                                                                                                                      tGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGc                 >  1:2203170/1‑150 (MQ=255)
                                                                                                                                       gCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCt                <  2:4245509/150‑1 (MQ=255)
                                                                                                                                        cTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTa               <  1:2737440/150‑1 (MQ=255)
                                                                                                                                         tCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTAt              <  2:2300718/150‑1 (MQ=255)
                                                                                                                                          cACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTAtt             <  2:1326491/150‑1 (MQ=255)
                                                                                                                                          cACTTCATATCAGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTAtt             >  1:3171779/1‑150 (MQ=255)
                                                                                                                                           aCTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCAGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTg            <  2:4052928/150‑1 (MQ=255)
                                                                                                                                              tCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATc         <  1:2425820/150‑1 (MQ=255)
                                                                                                                                                atatCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAg       >  1:2826460/1‑150 (MQ=255)
                                                                                                                                                atatCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAg       <  2:4482919/150‑1 (MQ=255)
                                                                                                                                                atatCAGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAg       <  2:4047198/150‑1 (MQ=255)
                                                                                                                                                 tatCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCAAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGc      >  1:3477806/1‑150 (MQ=255)
                                                                                                                                                   tCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGccc    >  2:1067838/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TAACAGCAATGGCTTTGCCGGCCGTTGACCGCGCTTCACGGCGATATGCGTCACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTT  >  CP009273/1755587‑1755885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑