Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 126,784 C→A 38.7% D322Y (GAT→TAT)  yacH ← uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273126,7840CA38.7% 315.0 / 248.7 232D322Y (GAT→TAT) yacHuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (73/69);  new base A (39/51);  total (112/120)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 235 total aligned reads displayed.

GGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTG  >  CP009273/126635‑126933
                                                                                                                                                     |                                                                                                                                                     
    acacCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCgtgg                                                                                                                                                   >  1:2897301/1‑150 (MQ=255)
     cacCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAgtggt                                                                                                                                                  <  2:1811571/150‑1 (MQ=255)
        cATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGtgat                                                                                                                                               >  1:1620273/1‑150 (MQ=255)
        cATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGtgat                                                                                                                                               <  1:446854/150‑1 (MQ=255)
        cATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGtgat                                                                                                                                               <  2:1747621/150‑1 (MQ=255)
          ttACGGTAGTTTGGATTGTGCCTCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGtgatga                                                                                                                                             >  1:375208/1‑150 (MQ=255)
           tACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTgatgat                                                                                                                                            <  1:1852097/150‑1 (MQ=255)
           tACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTgatgat                                                                                                                                            <  1:3384379/150‑1 (MQ=255)
            aCGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATa                                                                                                                                           >  2:1195710/1‑150 (MQ=255)
            aCGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATa                                                                                                                                           >  1:2720084/1‑150 (MQ=255)
            aCGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATa                                                                                                                                           >  1:402705/1‑150 (MQ=255)
             cGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATaa                                                                                                                                          >  1:892712/1‑150 (MQ=255)
              ggTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAat                                                                                                                                         <  2:2169750/150‑1 (MQ=255)
              ggTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAat                                                                                                                                         <  2:3073504/150‑1 (MQ=255)
              ggTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAat                                                                                                                                         <  2:3265951/150‑1 (MQ=255)
              ggTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAat                                                                                                                                         <  2:3478158/150‑1 (MQ=255)
                 aGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAatcat                                                                                                                                      >  1:2083287/1‑150 (MQ=255)
                 aGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAatcat                                                                                                                                      >  1:3151122/1‑150 (MQ=255)
                  gTTTGGATTGTGCCTCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAatcatc                                                                                                                                     <  2:2559323/150‑1 (MQ=255)
                   tttGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAAtcatca                                                                                                                                    <  1:2542967/150‑1 (MQ=255)
                       gATTGTGCCTCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGt                                                                                                                                <  2:1406907/150‑1 (MQ=255)
                        aTTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTc                                                                                                                               >  1:492550/1‑150 (MQ=255)
                            tgCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCatga                                                                                                                           >  1:1094314/1‑150 (MQ=255)
                             gccgccATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGAACGCCATAGTGGTGATGATAATCATCATTGTCatgat                                                                                                                          <  2:3007346/150‑1 (MQ=255)
                                    tgccatATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCatgatgatgatg                                                                                                                   >  2:132368/1‑150 (MQ=255)
                                     gccatATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATgg                                                                                                                  <  2:709252/150‑1 (MQ=255)
                                     gccatATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATgg                                                                                                                  <  1:3464132/150‑1 (MQ=255)
                                        atatTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCa                                                                                                               >  2:409798/1‑150 (MQ=255)
                                          atTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATg                                                                                                             <  1:1873874/150‑1 (MQ=255)
                                            tCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAt                                                                                                           <  1:2843025/150‑1 (MQ=255)
                                                    gTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGAtcgtcgtcg                                                                                                   >  1:2797482/1‑150 (MQ=255)
                                                    gTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTTTCATGATGATGATGGTCATGAtcgtcgtcg                                                                                                   <  1:4288678/150‑1 (MQ=255)
                                                     tAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATcgtcgtcgt                                                                                                  <  2:3881180/150‑1 (MQ=255)
                                                      aaGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCgtcgtcgtc                                                                                                 <  1:3860463/150‑1 (MQ=255)
                                                      aaGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCgtcgtcgtc                                                                                                 <  2:1267975/150‑1 (MQ=255)
                                                         aTGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCa                                                                                              >  2:4357189/1‑150 (MQ=255)
                                                           gCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGt                                                                                            >  2:1955095/1‑150 (MQ=255)
                                                            cTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTc                                                                                           <  2:1542494/150‑1 (MQ=255)
                                                             tCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCg                                                                                          >  2:1625011/1‑150 (MQ=255)
                                                             tCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCg                                                                                          >  1:2052146/1‑150 (MQ=255)
                                                               aCCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGAt                                                                                        >  2:2630366/1‑150 (MQ=255)
                                                                 cGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGAtgc                                                                                      <  2:3199770/150‑1 (MQ=255)
                                                                  ggTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGAtgct                                                                                     <  2:3038651/150‑1 (MQ=255)
                                                                     gATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATgctgct                                                                                  <  1:3158776/150‑1 (MQ=255)
                                                                      aTACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGctgctg                                                                                 >  1:198740/1‑150 (MQ=255)
                                                                      aTACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGctgctg                                                                                 >  1:2515563/1‑150 (MQ=255)
                                                                       tACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGa                                                                                <  1:4077702/150‑1 (MQ=255)
                                                                          ggTTGAAATTGTTTACGACGATATTGAAGTTGTCCCCGTTCTGATGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGAAGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCTTCACAGTCGATGCTGCTGAATa                                                                             >  2:3306855/1‑150 (MQ=255)
                                                                          ggTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATa                                                                             <  1:1312240/150‑1 (MQ=255)
                                                                                  ttgttgACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTAc                                                                     <  2:3228477/150‑1 (MQ=255)
                                                                                  ttgttgACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTAc                                                                     <  2:4229388/150‑1 (MQ=255)
                                                                                    gttgACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGt                                                                   <  1:2836532/150‑1 (MQ=255)
                                                                                      tgACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTgg                                                                 >  2:3843410/1‑150 (MQ=255)
                                                                                      ngACGTCGATATAGATNTTGTCGCCGTTGTGATGCCAGCCATAACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATNGTCGTCGTCCCAGTCGATGCTGCTGAATAGTTCGTACGTgg                                                                 >  2:1904066/2‑150 (MQ=255)
                                                                                       gACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCTCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGt                                                                <  2:3979497/150‑1 (MQ=255)
                                                                                       gACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGt                                                                <  2:480734/150‑1 (MQ=255)
                                                                                       gACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGt                                                                <  2:670036/150‑1 (MQ=255)
                                                                                       gACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGt                                                                >  1:1753699/1‑150 (MQ=255)
                                                                                        aCGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTa                                                               <  2:2607408/150‑1 (MQ=255)
                                                                                         cGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAg                                                              <  2:1522801/150‑1 (MQ=255)
                                                                                            cGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCaa                                                           >  2:1976518/1‑150 (MQ=255)
                                                                                              atcttGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGTTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACg                                                         <  2:3559718/147‑1 (MQ=255)
                                                                                              atatTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACg                                                         >  2:2490080/1‑150 (MQ=255)
                                                                                                 ttGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGccc                                                      >  2:1040363/1‑150 (MQ=255)
                                                                                                  tGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCa                                                     >  2:4481760/1‑150 (MQ=255)
                                                                                                   gATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCAt                                                    <  2:3168505/150‑1 (MQ=255)
                                                                                                   gATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCAt                                                    <  1:2848755/150‑1 (MQ=255)
                                                                                                    aTGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATg                                                   >  2:476349/1‑150 (MQ=255)
                                                                                                    aTGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATg                                                   >  1:155528/1‑150 (MQ=255)
                                                                                                    aTGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATg                                                   <  2:892712/150‑1 (MQ=255)
                                                                                                     tgttgtCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGc                                                  >  2:4162527/1‑150 (MQ=255)
                                                                                                      gttgtCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCt                                                 >  1:1023512/1‑150 (MQ=255)
                                                                                                      gttgtCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCt                                                 <  1:4584011/150‑1 (MQ=255)
                                                                                                        tgtCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCtat                                               <  1:235139/150‑1 (MQ=255)
                                                                                                            gCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGcc                                           >  2:3791005/1‑150 (MQ=255)
                                                                                                            gCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGcc                                           <  1:2490890/150‑1 (MQ=255)
                                                                                                              cGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGa                                         >  1:256307/1‑150 (MQ=255)
                                                                                                                ttGTGTTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAAt                                       >  1:1740335/1‑150 (MQ=255)
                                                                                                                  gtgtTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATTATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATcc                                     <  2:2819641/150‑1 (MQ=255)
                                                                                                                  gtgtTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATcc                                     <  1:994571/150‑1 (MQ=255)
                                                                                                                   ttttGCCAGCCATTACCGTCACTATTACCGACATAGTGGTGAGGATAATCAGCATTGTCATGATGATGATGTTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCcg                                    >  2:1471685/3‑150 (MQ=255)
                                                                                                                   tgtTGCCAGCCATTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCcg                                    >  2:1350952/1‑150 (MQ=255)
                                                                                                                       gccagccaTTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTa                                >  1:4199642/1‑150 (MQ=255)
                                                                                                                        ccagccaTTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTAc                               >  1:904901/1‑150 (MQ=255)
                                                                                                                         cagccaTTACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACa                              >  2:4503743/1‑150 (MQ=255)
                                                                                                                          agccaTTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTAATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACaa                             >  1:3548288/1‑150 (MQ=255)
                                                                                                                               ttACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTg                        <  2:3696585/150‑1 (MQ=255)
                                                                                                                                tACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGt                       <  1:4141826/150‑1 (MQ=255)
                                                                                                                                tACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGt                       <  1:828445/150‑1 (MQ=255)
                                                                                                                                tACCGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGt                       >  1:394726/1‑150 (MQ=255)
                                                                                                                                  ccGTCACGATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTca                     <  1:1495889/150‑1 (MQ=255)
                                                                                                                                  ccGACACGAGGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTca                     <  2:188532/150‑1 (MQ=255)
                                                                                                                                    gTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTcaac                   >  1:2185030/1‑150 (MQ=255)
                                                                                                                                         gATGACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACg              >  2:396786/1‑150 (MQ=255)
                                                                                                                                            gACCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGtt           <  2:2751073/150‑1 (MQ=255)
                                                                                                                                             aCCGCCATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTtc          <  2:4184677/150‑1 (MQ=255)
                                                                                                                                              ccGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTtct         <  2:2046104/150‑1 (MQ=255)
                                                                                                                                                gCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTcc       <  2:1432161/150‑1 (MQ=255)
                                                                                                                                                 ccATAGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCg      <  1:2142933/150‑1 (MQ=255)
                                                                                                                                                   aTCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGc    >  1:1641778/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGGCACACCATTACGGTAGTTTGGATTGTGCCGCCATGCCATATTCTTATCAGTAAGATGCTCACCGGTGATACGGTTGAAATTGTTGACGTCGATATTGATGTTGTCGCCGTTGTGTTGCCAGCCATTACCGTCACGATGACCGCCATCGTGGTGATGATAATCATCATTGTCATGATGATGATGGTCATGATCGTCGTCGTCCCAGTCGATGCTGCTGAATAGTGCGTACGTGGTAGCAACGCCCATGCTATAGCCGAATCCGCGTACAAAGCTGTCAACAAACGGTTCTCCGGCTG  >  CP009273/126635‑126933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑