Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,941,921 A→T 15.3% V163E (GTG→GAG)  yebC ← UPF0082 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,941,9210AT15.3% 691.0 / 105.5 247V163E (GTG→GAG) yebCUPF0082 family protein
Reads supporting (aligned to +/- strand):  ref base A (103/106);  new base T (16/22);  total (119/128)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01

Only 100 of 255 total aligned reads displayed.

CGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACG  >  CP009273/1941772‑1942069
                                                                                                                                                     |                                                                                                                                                    
cGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTca                                                                                                                                                      <  1:2205827/150‑1 (MQ=255)
 ggTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTcac                                                                                                                                                     >  1:783103/1‑150 (MQ=255)
    ttCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCTCAAc                                                                                                                                                  <  1:4011740/150‑1 (MQ=255)
     tCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGACTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGAGTAGACATCAATCGCGCCGTCATCATAGGTCACAACg                                                                                                                                                 >  1:4636807/1‑150 (MQ=255)
       tGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTc                                                                                                                                               <  1:1774416/150‑1 (MQ=255)
        gCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCt                                                                                                                                              >  2:1610717/1‑150 (MQ=255)
        gCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCt                                                                                                                                              >  1:3534177/1‑150 (MQ=255)
         cATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCTCAACGTCtt                                                                                                                                             >  2:888338/1‑150 (MQ=255)
             cATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGc                                                                                                                                         <  1:3356402/150‑1 (MQ=255)
              atatCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCGTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCa                                                                                                                                        >  2:2116589/1‑150 (MQ=255)
                atCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCAcc                                                                                                                                      >  2:706456/1‑150 (MQ=255)
                atCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCAcc                                                                                                                                      >  2:3744080/1‑150 (MQ=255)
                atCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCAcc                                                                                                                                      <  1:3348040/150‑1 (MQ=255)
                 tCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCt                                                                                                                                     <  2:3408149/150‑1 (MQ=255)
                     cTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCtt                                                                                                                                 <  2:4209691/150‑1 (MQ=255)
                        tGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCATTGTAGACATCAATCGCGCCGTCATCATAGGTCAAAACGTCATCAGCACCTGCTTCCa                                                                                                                              >  1:182096/1‑150 (MQ=255)
                           tAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGtg                                                                                                                           >  1:3546810/1‑150 (MQ=255)
                            aGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGtgc                                                                                                                          >  1:1384803/1‑150 (MQ=255)
                                 ggATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCtt                                                                                                                     >  2:1173634/1‑150 (MQ=255)
                                  gATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTc                                                                                                                    <  2:2453585/150‑1 (MQ=255)
                                  gATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTc                                                                                                                    >  2:4220865/1‑150 (MQ=255)
                                    tCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCa                                                                                                                  <  1:1948079/150‑1 (MQ=255)
                                    tCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCa                                                                                                                  <  1:3722031/150‑1 (MQ=255)
                                     cATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCat                                                                                                                 >  2:1037439/1‑150 (MQ=255)
                                      aTGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCatg                                                                                                                >  2:887761/1‑150 (MQ=255)
                                      aTGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCatg                                                                                                                <  2:3942306/150‑1 (MQ=255)
                                      aTGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCatg                                                                                                                >  2:4169034/1‑150 (MQ=255)
                                      aTGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCAGAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCatg                                                                                                                >  2:1924294/1‑150 (MQ=255)
                                       tGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCatga                                                                                                               >  2:4373005/1‑150 (MQ=255)
                                        ggAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCatgat                                                                                                              <  2:1674054/150‑1 (MQ=255)
                                               tCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCt                                                                                                       >  2:2480635/1‑150 (MQ=255)
                                                   cgcTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCAt                                                                                                   <  2:453100/150‑1 (MQ=255)
                                                    gcTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATc                                                                                                  <  1:4525453/150‑1 (MQ=255)
                                                    gcTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATc                                                                                                  <  2:3549975/150‑1 (MQ=255)
                                                    gcTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATc                                                                                                  <  1:4094254/150‑1 (MQ=255)
                                                     cTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCg                                                                                                 <  2:3995213/150‑1 (MQ=255)
                                                         cTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCtt                                                                                             >  1:4483732/1‑150 (MQ=255)
                                                          tGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCttt                                                                                            >  2:668987/1‑150 (MQ=255)
                                                           gCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTtc                                                                                           <  2:3081529/150‑1 (MQ=255)
                                                                 aGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAg                                                                                     >  2:354649/1‑150 (MQ=255)
                                                                    ccTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGgag                                                                                  <  2:2680278/150‑1 (MQ=255)
                                                                       gCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATc                                                                               <  2:1125463/150‑1 (MQ=255)
                                                                       gCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATc                                                                               <  2:919438/150‑1 (MQ=255)
                                                                        ccGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCa                                                                              >  2:4262225/1‑150 (MQ=255)
                                                                        ccGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCa                                                                              <  1:663161/150‑1 (MQ=255)
                                                                         cGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCAc                                                                             >  2:2252995/1‑150 (MQ=255)
                                                                              ccAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACGACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCtt                                                                        <  1:2407568/150‑1 (MQ=255)
                                                                               cAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCttt                                                                       >  1:423949/1‑150 (MQ=255)
                                                                                     gTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCt                                                                 <  1:3294880/150‑1 (MQ=255)
                                                                                      tCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTg                                                                >  1:3808737/1‑150 (MQ=255)
                                                                                           cACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAg                                                           <  2:165421/150‑1 (MQ=255)
                                                                                            aCTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGa                                                          >  2:2512427/1‑150 (MQ=255)
                                                                                            aCTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGa                                                          >  1:3907025/1‑150 (MQ=255)
                                                                                              tttACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATa                                                        >  2:3055063/1‑150 (MQ=255)
                                                                                              tttACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATa                                                        >  2:1457924/1‑150 (MQ=255)
                                                                                               ttACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAg                                                       >  1:3238168/1‑150 (MQ=255)
                                                                                                tACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAgg                                                      >  2:1510421/1‑150 (MQ=255)
                                                                                                  cccATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCa                                                    <  1:1114715/150‑1 (MQ=255)
                                                                                                   ccATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCaa                                                   >  1:1365607/1‑150 (MQ=255)
                                                                                                    cATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAAc                                                  <  1:4219980/150‑1 (MQ=255)
                                                                                                      tttCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACgg                                                >  1:3546031/1‑150 (MQ=255)
                                                                                                      tttCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACgg                                                <  1:3457209/150‑1 (MQ=255)
                                                                                                        tcttcCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGaa                                              <  1:2211351/150‑1 (MQ=255)
                                                                                                        tcttcCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCTAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGaa                                              >  2:3915910/1‑150 (MQ=255)
                                                                                                         cttcCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAAc                                             >  1:2883518/1‑150 (MQ=255)
                                                                                                         cttcCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAAc                                             >  1:1514473/1‑150 (MQ=255)
                                                                                                          ttcCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAAcc                                            <  2:233831/150‑1 (MQ=255)
                                                                                                          ttcCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAAcc                                            >  1:1131034/1‑150 (MQ=255)
                                                                                                          ttcCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAAcc                                            <  2:3725707/150‑1 (MQ=255)
                                                                                                            cccATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCAt                                          <  2:2083956/150‑1 (MQ=255)
                                                                                                              cATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCa                                        <  1:596335/150‑1 (MQ=255)
                                                                                                              cATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCa                                        <  2:3168835/150‑1 (MQ=255)
                                                                                                               aTGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAg                                       <  2:4044997/150‑1 (MQ=255)
                                                                                                                tGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGt                                      >  1:3177291/1‑150 (MQ=255)
                                                                                                                 gCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTa                                     >  2:1849800/1‑150 (MQ=255)
                                                                                                                  cAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTAc                                    <  1:974225/150‑1 (MQ=255)
                                                                                                                   aGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTAcc                                   >  1:2601430/1‑150 (MQ=255)
                                                                                                                   aGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTAcc                                   >  1:2908313/1‑150 (MQ=255)
                                                                                                                   aGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTAcc                                   <  1:706456/150‑1 (MQ=255)
                                                                                                                    gtgtAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCg                                  <  2:783103/150‑1 (MQ=255)
                                                                                                                    gtgtAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCg                                  <  2:2903095/150‑1 (MQ=255)
                                                                                                                      gtAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAg                                <  1:1600348/150‑1 (MQ=255)
                                                                                                                      gtAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAg                                <  1:6342/150‑1 (MQ=255)
                                                                                                                       tAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAgg                               <  1:3042579/150‑1 (MQ=255)
                                                                                                                             tCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCg                         >  2:228691/1‑150 (MQ=255)
                                                                                                                             tCAATCGCGCAGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGTTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACTGATAGGCAACGGAACCTTCAGTACCGAGGTTACCg                         <  1:2695198/150‑1 (MQ=255)
                                                                                                                                aTCGCGCCGTCATCATAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCca                      >  1:4003392/1‑150 (MQ=255)
                                                                                                                                       cGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGc               >  1:2500822/1‑150 (MQ=255)
                                                                                                                                          catcatAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTaa            >  1:4340725/1‑150 (MQ=255)
                                                                                                                                          catcatAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTaa            >  1:409892/1‑150 (MQ=255)
                                                                                                                                            tcatAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAAt          >  2:3928493/1‑150 (MQ=255)
                                                                                                                                            tcatAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAAt          >  2:3349339/1‑150 (MQ=255)
                                                                                                                                              atAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGc        >  1:1981686/1‑150 (MQ=255)
                                                                                                                                               tAGGTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCa       <  1:1302718/150‑1 (MQ=255)
                                                                                                                                               tAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATAGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAAAAGATAGGCAACGGAACCATCAGTAACGAGGTTACCTCCACATTTGATAAATTCa       >  1:3304251/1‑150 (MQ=255)
                                                                                                                                                aGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCAt      <  1:4112079/150‑1 (MQ=255)
                                                                                                                                                 ggTCTCAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATg     <  2:3253439/150‑1 (MQ=255)
                                                                                                                                                  gTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGa    >  1:1867626/1‑150 (MQ=255)
                                                                                                                                                  gTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGa    <  2:2591716/150‑1 (MQ=255)
                                                                                                                                                    cacaACGTCTTCAGCACCTGCTTCCAGTGCTGATTCCATTATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACAATCAGTACCGAGGTTAACGCCACATTTGCTAAATGCATGACg  <  1:1289000/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACG  >  CP009273/1941772‑1942069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑