Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 135,557 G→T 37.6% A719E (GCG→GAG)  gcd ← glucose dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273135,5570GT37.6% 422.3 / 295.1 284A719E (GCG→GAG) gcdglucose dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (78/99);  new base T (57/50);  total (135/149)
Fisher's exact test for biased strand distribution p-value = 1.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 291 total aligned reads displayed.

ACATACTGCTTACCATTCACTTCATAGGTCATTGGC‑GTAGCCTGACCACCCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCC  >  CP009273/135410‑135706
                                                                                                                                                     |                                                                                                                                                     
agattcTGCTTACCATTCACTTCATAGTTCATTGGC‑GTAGCCTGACAACCCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCTTAGCGGCGATAAACAGCACGTTACccgcc                                                                                                                                                     <  1:3661390/145‑1 (MQ=255)
acaTACTGCTTACCATTCACTTCATAGGTCATTGGC‑GTAGCCTGACCACCCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACccgcc                                                                                                                                                     >  1:982300/1‑150 (MQ=255)
  aTACTGCTTACCATTCACTTCATAGGTCATTGGC‑GTAGCCTGACCACCCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGt                                                                                                                                                   >  2:2556834/1‑150 (MQ=255)
   tACTGCTTACCATTCACTTCATAGGTCATTGGC‑GTAGCCTGACCACCCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTg                                                                                                                                                  >  1:951715/1‑150 (MQ=255)
              aTTCACTTCATAGGTCATTGGC‑GTAGCCTGACCACCCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGgccc                                                                                                                                       <  1:3912615/150‑1 (MQ=255)
              aTTCACTTCATAGGTCATTGGC‑GTAGCCTGACCACCCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGgccc                                                                                                                                       <  1:682648/150‑1 (MQ=255)
              aTTCACTTCATAGGTCATTGGC‑GTAGCCTGACCACCCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGgccc                                                                                                                                       <  2:4482151/150‑1 (MQ=255)
                  aCTTCATAGGTCATTGGC‑GTAGCCTGACCACCCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGgcccgccc                                                                                                                                   >  2:4494180/1‑150 (MQ=255)
                   cTTCATAGGTCATTGGC‑GTAGCCTGACCACCCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCa                                                                                                                                  <  2:3493208/150‑1 (MQ=255)
                    ttCATAGGTCATTGGC‑GTAGCCTGACCACCCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAg                                                                                                                                 >  2:3386747/1‑150 (MQ=255)
                      cATAGGTCATTGGC‑GTAGCCTGACCACCCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCa                                                                                                                               >  2:1121585/1‑150 (MQ=255)
                        tAGGTCATTGGC‑GTAGCCTGACCACCCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATc                                                                                                                             <  1:3727932/150‑1 (MQ=255)
                        tAGGTCATTGGC‑GTAGCCTGACCACCCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATc                                                                                                                             >  2:51714/1‑150 (MQ=255)
                         aGGTCATTGGC‑GTAGCCTGACCACCCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCg                                                                                                                            >  2:1520464/1‑150 (MQ=255)
                           gTCATTGGC‑GTAGCCTGACCACCCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACTTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGc                                                                                                                          >  2:23068/1‑150 (MQ=255)
                              cttGGCGGTAGCCGGACCCCCCCCGGGAAACCGA‑CCTGGCACCAGTTTTCACCGTTGCTCCTGTTGGAACCGGCGATGTTCTTTTTCGGCCTTCGC‑GCGCAAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATa                                                                                                                       <  2:940957/149‑1 (MQ=255)
                              aTTGGC‑GTAGCCTGACCACCCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATa                                                                                                                       >  1:110575/1‑150 (MQ=255)
                                     gTAGCCTGACCACCCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCata                                                                                                                 <  1:3313170/150‑1 (MQ=255)
                                       aGCCTGACCACCCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATAtt                                                                                                               >  1:4388965/1‑150 (MQ=255)
                                          cTGACCACCCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGaa                                                                                                            >  2:2201487/1‑150 (MQ=255)
                                            gACCACCCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACg                                                                                                          <  2:2488365/150‑1 (MQ=255)
                                             accaccCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACgg                                                                                                         <  2:3569890/150‑1 (MQ=255)
                                               caccCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCa                                                                                                       >  2:954277/1‑150 (MQ=255)
                                                 cccGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCAcc                                                                                                     <  1:1050021/150‑1 (MQ=255)
                                                 cccGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCAcc                                                                                                     <  1:4500943/150‑1 (MQ=255)
                                                 cccGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCAcc                                                                                                     >  1:4245294/1‑150 (MQ=255)
                                                       ggTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAAcc                                                                                               <  2:1277241/150‑1 (MQ=255)
                                                        gTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCg                                                                                              <  1:4259655/150‑1 (MQ=255)
                                                           aaCGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCa                                                                                           <  1:615620/150‑1 (MQ=255)
                                                            aCGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCAt                                                                                          >  2:3080723/1‑150 (MQ=255)
                                                            aCGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCAt                                                                                          <  2:782911/150‑1 (MQ=255)
                                                            aCGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCAt                                                                                          >  2:3975792/1‑150 (MQ=255)
                                                             cGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATc                                                                                         >  1:4543619/1‑150 (MQ=255)
                                                              gACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCg                                                                                        >  2:1198380/1‑150 (MQ=255)
                                                                 ccTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGa                                                                                     >  1:4484556/1‑150 (MQ=255)
                                                                     ccACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATGGAACGGCACCGGAACCGGCATCGGGAACgg                                                                                 <  1:2379684/150‑1 (MQ=255)
                                                                         aGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATa                                                                             <  2:2413655/150‑1 (MQ=255)
                                                                          gTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATAc                                                                            >  1:2155364/1‑150 (MQ=255)
                                                                           tttttCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACt                                                                           >  1:3359305/1‑150 (MQ=255)
                                                                            ttttCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTTCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCTGAAACGTCATCGGGAACGGCATACTg                                                                          >  1:352923/1‑150 (MQ=255)
                                                                            ttttCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTg                                                                          <  2:364454/150‑1 (MQ=255)
                                                                              ttCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTc                                                                        >  2:379276/1‑150 (MQ=255)
                                                                              ttCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTc                                                                        <  1:2572264/150‑1 (MQ=255)
                                                                              ttCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTc                                                                        <  2:1778873/150‑1 (MQ=255)
                                                                              ttCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTc                                                                        >  2:2552294/1‑150 (MQ=255)
                                                                                 aCCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGACCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTg                                                                     >  2:488280/1‑150 (MQ=255)
                                                                                  ccGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTgc                                                                    <  2:4509701/150‑1 (MQ=255)
                                                                                   cGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGTACGGCATACTGTCCTgcg                                                                   <  2:9102/150‑1 (MQ=255)
                                                                                     ttGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTgcggc                                                                 <  2:233505/150‑1 (MQ=255)
                                                                                      tGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTgcggcg                                                                >  1:2335656/1‑150 (MQ=255)
                                                                                      tGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTgcggcg                                                                <  1:4137945/150‑1 (MQ=255)
                                                                                       gCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGt                                                               >  2:828904/1‑150 (MQ=255)
                                                                                        cTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTa                                                              >  1:1832140/1‑150 (MQ=255)
                                                                                         tCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTAc                                                             >  1:3050079/1‑150 (MQ=255)
                                                                                          cATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTAcc                                                            >  2:4281316/1‑150 (MQ=255)
                                                                                              ttgtAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATa                                                        <  2:394244/150‑1 (MQ=255)
                                                                                              ttgtAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATa                                                        <  1:814503/150‑1 (MQ=255)
                                                                                                gtAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACg                                                      <  2:1588559/150‑1 (MQ=255)
                                                                                                 tAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGt                                                     >  2:2107457/1‑150 (MQ=255)
                                                                                                  aaGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGtt                                                    <  1:201010/150‑1 (MQ=255)
                                                                                                   aGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGttt                                                   <  2:2588326/150‑1 (MQ=255)
                                                                                                   aGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGttt                                                   <  2:3433107/150‑1 (MQ=255)
                                                                                                   aGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGttt                                                   >  2:893306/1‑150 (MQ=255)
                                                                                                    gc‑gcgcAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTttc                                                  <  1:3812535/150‑1 (MQ=255)
                                                                                                     c‑gcgcAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTttct                                                 <  1:3237197/150‑1 (MQ=255)
                                                                                                       gcgcTGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTttctt                                                <  2:4133450/150‑1 (MQ=255)
                                                                                                       gcgcAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTttctt                                                >  2:3585073/1‑150 (MQ=255)
                                                                                                       gcgcAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTttctt                                                <  1:2598521/150‑1 (MQ=255)
                                                                                                         gcAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTcc                                              >  2:2521479/1‑150 (MQ=255)
                                                                                                           aGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCcac                                            >  2:1438832/1‑150 (MQ=255)
                                                                                                           aGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCcac                                            >  2:3222718/1‑150 (MQ=255)
                                                                                                           aGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCcac                                            >  1:1343627/1‑150 (MQ=255)
                                                                                                           aGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCcac                                            <  1:2877369/150‑1 (MQ=255)
                                                                                                           aGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCcac                                            <  1:3154052/150‑1 (MQ=255)
                                                                                                                 ttATCTGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACt                                      <  1:2465974/150‑1 (MQ=255)
                                                                                                                      tGCCGTAGCGGCGATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCAtt                                 <  2:2666745/150‑1 (MQ=255)
                                                                                                                      tGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCAtt                                 >  1:513924/1‑150 (MQ=255)
                                                                                                                       gCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTa                                >  1:1809253/1‑150 (MQ=255)
                                                                                                                       gCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTa                                <  2:1690210/150‑1 (MQ=255)
                                                                                                                         cGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGGTTCTTCCACACCACTTCATTAGt                              >  2:2013462/1‑150 (MQ=255)
                                                                                                                             gcggcgATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTc                          >  1:3691138/1‑150 (MQ=255)
                                                                                                                              cggcgATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCa                         <  2:662934/150‑1 (MQ=255)
                                                                                                                              cggcgATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCa                         <  2:2833786/150‑1 (MQ=255)
                                                                                                                                 cgATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGAt                      >  2:2920456/1‑150 (MQ=255)
                                                                                                                                 cgATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGAt                      <  2:584612/150‑1 (MQ=255)
                                                                                                                                  gATAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATc                     >  2:1940907/1‑150 (MQ=255)
                                                                                                                                  gATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATc                     <  1:3418158/150‑1 (MQ=255)
                                                                                                                                   aTAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATcc                    <  2:3025868/150‑1 (MQ=255)
                                                                                                                                   aTAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGTATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATcc                    >  1:1649831/1‑150 (MQ=255)
                                                                                                                                    tAAACAGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCa                   <  1:2754620/150‑1 (MQ=255)
                                                                                                                                       aCAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAgcg                <  1:2223639/150‑1 (MQ=255)
                                                                                                                                        cAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAgcgc               <  2:1368592/150‑1 (MQ=255)
                                                                                                                                         aGCACGTTACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGcc              >  2:3898477/1‑150 (MQ=255)
                                                                                                                                         aGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGcc              <  1:2111376/150‑1 (MQ=255)
                                                                                                                                         aGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGcc              <  1:794958/150‑1 (MQ=255)
                                                                                                                                         aGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACAAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGcc              >  2:1821309/1‑150 (MQ=255)
                                                                                                                                          gCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCg             <  1:4168260/150‑1 (MQ=255)
                                                                                                                                             cGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCAATAGTTTTCAGATCCAGCGCCgaga          >  2:1003242/1‑150 (MQ=255)
                                                                                                                                                tACCCTCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGatat       >  1:1863991/1‑150 (MQ=255)
                                                                                                                                                    cgccgTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCTTACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAAcc   <  1:3440582/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACATACTGCTTACCATTCACTTCATAGGTCATTGGC‑GTAGCCTGACCACCCGCTGGTAAACGACCCTGCCACAGTTTTTCACCGTTGCTCATGTTGTAAGC‑GCGCAGGTAGTTATCTGCCGTAGCGGCGATAAACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCC  >  CP009273/135410‑135706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑