Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,943,414 C→A 14.2% D456Y (GAT→TAT)  aspS ← aspartyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,943,4140CA14.2% 855.5 / 80.7 253D456Y (GAT→TAT) aspSaspartyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (108/109);  new base A (18/18);  total (126/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

Only 100 of 259 total aligned reads displayed.

GATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTGCACCCA  >  CP009273/1943265‑1943563
                                                                                                                                                     |                                                                                                                                                     
gATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATc                                                                                                                                                       <  2:1455243/150‑1 (MQ=255)
 aTACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCt                                                                                                                                                      >  2:3871481/1‑150 (MQ=255)
   aCCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCttt                                                                                                                                                    <  1:1926780/150‑1 (MQ=255)
    cccAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTc                                                                                                                                                   <  1:3390790/150‑1 (MQ=255)
     ccAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCg                                                                                                                                                  <  2:3324866/150‑1 (MQ=255)
     ccAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCg                                                                                                                                                  <  2:3219440/150‑1 (MQ=255)
        gAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCggtg                                                                                                                                               >  1:3686727/1‑150 (MQ=255)
          aTACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGGCATATATTTCggtgag                                                                                                                                             >  1:669019/1‑150 (MQ=255)
              cAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCggtgaggtga                                                                                                                                         >  1:1809485/1‑150 (MQ=255)
              cAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCggtgaggtga                                                                                                                                         >  2:1014877/1‑150 (MQ=255)
               aaaCACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGaa                                                                                                                                        >  1:4011582/1‑150 (MQ=255)
               aaaCACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGaa                                                                                                                                        <  2:4612096/150‑1 (MQ=255)
                 acacCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACg                                                                                                                                      >  2:3194955/1‑150 (MQ=255)
                     cGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATg                                                                                                                                  <  1:1632822/150‑1 (MQ=255)
                     cGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATg                                                                                                                                  >  2:533484/1‑150 (MQ=255)
                         tgctgcATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATATTTCGGTGAGGTGAACGGATGGTGc                                                                                                                              <  1:2280910/150‑1 (MQ=255)
                           ctgcATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCAt                                                                                                                            <  2:3079004/150‑1 (MQ=255)
                           ctgcATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCAt                                                                                                                            >  2:4231338/1‑150 (MQ=255)
                            tgcATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCAtt                                                                                                                           >  1:448309/1‑150 (MQ=255)
                            tgcATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCAtt                                                                                                                           <  1:4178835/150‑1 (MQ=255)
                            tgcATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCAtt                                                                                                                           >  1:1000221/1‑150 (MQ=255)
                            tgcATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCAtt                                                                                                                           <  1:129025/150‑1 (MQ=255)
                             gcATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTg                                                                                                                          <  2:4262396/150‑1 (MQ=255)
                               atatCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGcc                                                                                                                        <  2:1087078/150‑1 (MQ=255)
                               atatCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGGCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGcc                                                                                                                        >  1:4333007/1‑150 (MQ=255)
                                tatCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCg                                                                                                                       <  1:4315556/150‑1 (MQ=255)
                                   cACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCa                                                                                                                    >  2:1720941/1‑150 (MQ=255)
                                   cACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATATTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCa                                                                                                                    <  1:2195190/150‑1 (MQ=255)
                                     ccATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAgg                                                                                                                  >  1:3222782/1‑150 (MQ=255)
                                        ttATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATCTCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGgccg                                                                                                               >  2:2087688/1‑150 (MQ=255)
                                        ttATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGgccg                                                                                                               <  2:1036835/150‑1 (MQ=255)
                                          aTGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGgccgcc                                                                                                             >  2:1623069/1‑150 (MQ=255)
                                          aTGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATATTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGgccgcc                                                                                                             <  2:809067/150‑1 (MQ=255)
                                           tGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCt                                                                                                            <  2:3689998/150‑1 (MQ=255)
                                           tGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCt                                                                                                            <  2:4472053/150‑1 (MQ=255)
                                            ggATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCtt                                                                                                           >  1:934021/1‑150 (MQ=255)
                                                aCGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCAcc                                                                                                       <  2:2153139/150‑1 (MQ=255)
                                                aCGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCAcc                                                                                                       >  2:3932423/1‑150 (MQ=255)
                                                    aCTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACcgtcg                                                                                                   <  1:3442278/150‑1 (MQ=255)
                                                        aaCCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCtt                                                                                               <  1:620999/150‑1 (MQ=255)
                                                         accaccGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATATTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTc                                                                                              <  1:4509998/150‑1 (MQ=255)
                                                         accaccGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATATTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTc                                                                                              <  2:1528243/150‑1 (MQ=255)
                                                              cGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACa                                                                                         <  2:2914644/150‑1 (MQ=255)
                                                              cGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACa                                                                                         >  1:2796096/1‑150 (MQ=255)
                                                               gCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACAt                                                                                        <  2:3164078/150‑1 (MQ=255)
                                                                cccACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATc                                                                                       >  1:802306/1‑150 (MQ=255)
                                                                cccACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATATTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATc                                                                                       >  2:1169858/1‑150 (MQ=255)
                                                                 ccACTTCGTAACCATTGATGACCATATCGTACGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCg                                                                                      <  2:3993654/150‑1 (MQ=255)
                                                                   aCTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCggg                                                                                    <  1:1398498/150‑1 (MQ=255)
                                                                   aCTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCggg                                                                                    <  1:2095480/150‑1 (MQ=255)
                                                                    cTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATATTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGa                                                                                   <  1:3097440/150‑1 (MQ=255)
                                                                     ttCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGaa                                                                                  <  1:1011472/150‑1 (MQ=255)
                                                                     ttCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGaa                                                                                  <  1:1353225/150‑1 (MQ=255)
                                                                       cGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGt                                                                                >  2:594967/1‑150 (MQ=255)
                                                                       cGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGt                                                                                >  2:2091182/1‑150 (MQ=255)
                                                                       cGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATATTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGt                                                                                >  2:1627983/1‑150 (MQ=255)
                                                                        gTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATATTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTc                                                                               >  1:1290030/1‑150 (MQ=255)
                                                                            ccATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATa                                                                           >  1:1157995/1‑150 (MQ=255)
                                                                            ccATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATa                                                                           <  2:2219842/150‑1 (MQ=255)
                                                                              aTTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAAc                                                                         >  2:404317/1‑150 (MQ=255)
                                                                                 gatgaCCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATATTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCca                                                                      <  2:2430756/150‑1 (MQ=255)
                                                                                     aCCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGc                                                                  <  2:1786538/150‑1 (MQ=255)
                                                                                     aCCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGc                                                                  >  1:843344/1‑150 (MQ=255)
                                                                                       cATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCgg                                                                >  2:2685832/1‑150 (MQ=255)
                                                                                       cATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATATTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCgg                                                                >  2:4493695/1‑150 (MQ=255)
                                                                                         tatCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTg                                                              <  2:103727/150‑1 (MQ=255)
                                                                                            cGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGccc                                                           >  1:2782671/1‑150 (MQ=255)
                                                                                              tAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCAt                                                         <  1:3871481/150‑1 (MQ=255)
                                                                                                aGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCAttt                                                       >  2:3340892/1‑150 (MQ=255)
                                                                                                   gTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATATTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCt                                                    >  1:2696412/1‑150 (MQ=255)
                                                                                                       gCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTtcg                                                <  1:4061691/150‑1 (MQ=255)
                                                                                                       gCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTtcg                                                >  2:660697/1‑150 (MQ=255)
                                                                                                         cACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTtcgtc                                              >  1:2599380/1‑150 (MQ=255)
                                                                                                          aCCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATCACCCACAGCGGTGCCCATTTGCTTtcgtcg                                             <  1:2380376/150‑1 (MQ=255)
                                                                                                          aCCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATATTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTtcgtcg                                             <  1:372871/150‑1 (MQ=255)
                                                                                                           ccGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCgg                                            <  2:2821141/150‑1 (MQ=255)
                                                                                                            cGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGt                                           <  2:3470305/150‑1 (MQ=255)
                                                                                                             gCAGTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCATGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGCTGACCATTTGCTTTCGTCGGTc                                          >  1:38696/1‑150 (MQ=255)
                                                                                                              cATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCa                                         >  2:3585745/1‑150 (MQ=255)
                                                                                                               aTTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAg                                        >  1:3591985/1‑150 (MQ=255)
                                                                                                               aTTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAg                                        <  1:4555786/150‑1 (MQ=255)
                                                                                                                 tttCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGAc                                      >  2:2588946/1‑150 (MQ=255)
                                                                                                                 tttCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGAc                                      <  2:3830940/150‑1 (MQ=255)
                                                                                                                       gTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGt                                >  2:442342/1‑150 (MQ=255)
                                                                                                                        tGCAGCTTTCAGTTCTGCAGCCGTCATATATTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTc                               >  1:1111955/1‑150 (MQ=255)
                                                                                                                         gCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCt                              <  1:301183/150‑1 (MQ=255)
                                                                                                                          cAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCtt                             >  2:3408302/1‑150 (MQ=255)
                                                                                                                           aGCTTTCAGTTCTGCAGCCGTCATATATTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCttt                            >  2:832403/1‑150 (MQ=255)
                                                                                                                            gCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTa                           >  2:1427505/1‑150 (MQ=255)
                                                                                                                             cTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTAc                          >  2:2793595/1‑150 (MQ=255)
                                                                                                                              tttCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTAcc                         <  1:2824939/150‑1 (MQ=255)
                                                                                                                              tttCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTAcc                         >  2:652082/1‑150 (MQ=255)
                                                                                                                               ttCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTAccc                        <  2:1273660/150‑1 (MQ=255)
                                                                                                                                   gTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACtt                    >  1:1521053/1‑150 (MQ=255)
                                                                                                                                    ttCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACttt                   <  2:4354352/150‑1 (MQ=255)
                                                                                                                                       tGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAg                <  1:974800/150‑1 (MQ=255)
                                                                                                                                              gTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGTCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGt         <  2:3554336/150‑1 (MQ=255)
                                                                                                                                              gTCATATCTTTCGGTGAGGTGAACGGATGGTGCAGTGTCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGt         <  2:2314561/150‑1 (MQ=255)
                                                                                                                                                   atCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTGCAcc    >  1:2230823/1‑150 (MQ=255)
                                                                                                                                                   atCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTGCAcc    >  1:2471800/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTGCACCCA  >  CP009273/1943265‑1943563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑