Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 144,698 C→A 35.3% A195A (GCG→GCT)  panC ← pantothenate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273144,6980CA35.3% 431.9 / 247.5 258A195A (GCG→GCT) panCpantothenate synthetase
Reads supporting (aligned to +/- strand):  ref base C (83/84);  new base A (50/41);  total (133/125)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 268 total aligned reads displayed.

TCTGAATATCATCGGCGCGGAAGCCTTTTTCATTCAGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGC  >  CP009273/144550‑144847
                                                                                                                                                    |                                                                                                                                                     
tCTGAATATCATCGGCGCGGAAGCCTTTTTCATTCAGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTccg                                                                                                                                                      <  2:3559020/150‑1 (MQ=255)
 cTGAATATCATCGGCGCGGAAGCCTTTTTCATTCAGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTccgc                                                                                                                                                     >  1:4444039/1‑150 (MQ=255)
 cTGAATATCATCGGCGCGGAAGCCTTTTTCATTCAGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTccgc                                                                                                                                                     <  2:1462540/150‑1 (MQ=255)
  tGAATATCATCGGCGCGGAAGCCTTTTTCATTCAGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTccgcc                                                                                                                                                    >  1:403069/1‑150 (MQ=255)
  tGAATATCATCGGCGCGGAAGCCTTTTTCATTCAGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTcagcc                                                                                                                                                    >  2:1478859/1‑150 (MQ=255)
    aaTATCATCGGCGCGGAAGCCTTTTTCATTCAGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGt                                                                                                                                                  >  2:3251427/1‑150 (MQ=255)
       atcatcGGCGCGGAAGCCTTTTTCATTCAGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAg                                                                                                                                               <  1:954984/150‑1 (MQ=255)
        tcatcGGCGCGGAAGCCTTTTTCATTCAGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGa                                                                                                                                              <  1:3614425/150‑1 (MQ=255)
          atcGGCGCGGAAGCCTTTTTCATTCAGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATa                                                                                                                                            >  1:2669488/1‑150 (MQ=255)
            cGGCGCGGAAGCCTTTTTCATTCAGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAAc                                                                                                                                          <  1:1504635/150‑1 (MQ=255)
             ggCGCGGAAGCCTTTTTCATTCAGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAAcc                                                                                                                                         >  2:4391215/1‑150 (MQ=255)
                gcgGAAGCCTTTTTCATTCAGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGtt                                                                                                                                      <  2:1024306/150‑1 (MQ=255)
                 cgGAAGCCTTTTTCATTCAGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTa                                                                                                                                     <  2:2899797/150‑1 (MQ=255)
                 cgGAAGCCTTTTTCATTCAGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGGTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTa                                                                                                                                     <  1:4368108/150‑1 (MQ=255)
                   gAAGCCTTTTTCATTCAGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACg                                                                                                                                   >  2:3123469/1‑150 (MQ=255)
                   gAAGCCTTTTTCATTCAGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACg                                                                                                                                   >  2:2769439/1‑150 (MQ=255)
                     aGCCTTTTTCATTCAGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACggg                                                                                                                                 >  2:3281873/1‑150 (MQ=255)
                          ttttCATTCAGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACtt                                                                                                                            <  1:180922/150‑1 (MQ=255)
                            ttcattcaGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAg                                                                                                                          >  2:2398866/1‑150 (MQ=255)
                            ttcattcaGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGAGCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAg                                                                                                                          <  1:2017703/150‑1 (MQ=255)
                                  caGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAg                                                                                                                    >  1:86889/1‑150 (MQ=255)
                                   aGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGa                                                                                                                   >  1:655450/1‑150 (MQ=255)
                                   aGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGa                                                                                                                   >  1:3378127/1‑150 (MQ=255)
                                   aGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGa                                                                                                                   <  2:1088204/150‑1 (MQ=255)
                                    gTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGAc                                                                                                                  <  2:1032041/150‑1 (MQ=255)
                                    gTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGAc                                                                                                                  <  2:3851834/150‑1 (MQ=255)
                                    gTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGAc                                                                                                                  <  1:3262363/150‑1 (MQ=255)
                                     ttCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGAcc                                                                                                                 >  1:377519/1‑150 (MQ=255)
                                      tCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCg                                                                                                                <  1:494932/150‑1 (MQ=255)
                                       cTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGt                                                                                                               >  2:1138468/1‑150 (MQ=255)
                                        ttGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTc                                                                                                              <  1:1652572/150‑1 (MQ=255)
                                          gCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCtt                                                                                                            >  1:1814403/1‑150 (MQ=255)
                                             ccGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAA‑TTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGgc                                                                                                        <  2:1560489/150‑1 (MQ=255)
                                                 aaTGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGgcgcg                                                                                                     >  2:2975462/1‑150 (MQ=255)
                                                 aaTGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGgcgcg                                                                                                     >  1:1050175/1‑150 (MQ=255)
                                                    ggTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCAt                                                                                                  >  2:1700001/1‑150 (MQ=255)
                                                    ggTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCAt                                                                                                  >  2:4029547/1‑150 (MQ=255)
                                                       aataatTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGGGCGCATAAt                                                                                               <  1:3215653/150‑1 (MQ=255)
                                                         taatTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTg                                                                                             <  2:2813845/150‑1 (MQ=255)
                                                         taatTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTg                                                                                             >  2:347444/1‑150 (MQ=255)
                                                         taatTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTg                                                                                             <  1:1301883/150‑1 (MQ=255)
                                                         taatTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTg                                                                                             <  1:4385037/150‑1 (MQ=255)
                                                         taatTTCATCGAGATCCCGGTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTg                                                                                             <  2:2130344/150‑1 (MQ=255)
                                                          aatTTCATCGAGATCCCGGTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTgg                                                                                            <  2:383660/150‑1 (MQ=255)
                                                           atTTCATCGAGATCCTGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGc                                                                                           >  2:2286528/1‑150 (MQ=255)
                                                                  cGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGa                                                                                    >  2:2050138/1‑150 (MQ=255)
                                                                   gagaTCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGAc                                                                                   >  2:1534574/1‑150 (MQ=255)
                                                                   gagaTCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGAc                                                                                   >  1:4330420/1‑150 (MQ=255)
                                                                    agaTCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACa                                                                                  <  2:4603345/150‑1 (MQ=255)
                                                                     gaTCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACaa                                                                                 <  1:2858676/150‑1 (MQ=255)
                                                                       tCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAAtc                                                                               >  1:2804689/1‑150 (MQ=255)
                                                                        cccGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAAtct                                                                              >  2:2002193/1‑150 (MQ=255)
                                                                          cGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCa                                                                            >  2:2561785/1‑150 (MQ=255)
                                                                           gTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCaa                                                                           >  2:1039432/1‑150 (MQ=255)
                                                                            ttCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAat                                                                          <  2:2628666/150‑1 (MQ=255)
                                                                            ttCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAat                                                                          >  2:1771502/1‑150 (MQ=255)
                                                                             tCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAata                                                                         <  1:600884/150‑1 (MQ=255)
                                                                              ccccAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAatat                                                                        <  2:2785857/150‑1 (MQ=255)
                                                                              ccccAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAatat                                                                        <  1:3928733/150‑1 (MQ=255)
                                                                                  aGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGaa                                                                    >  1:1532587/1‑150 (MQ=255)
                                                                                   gCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAg                                                                   >  2:645425/1‑150 (MQ=255)
                                                                                    ccTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGc                                                                  >  2:4158560/1‑150 (MQ=255)
                                                                                     cTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGcc                                                                 >  1:4264315/1‑150 (MQ=255)
                                                                                      tGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGccc                                                                <  1:3862432/150‑1 (MQ=255)
                                                                                      tGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGccc                                                                >  2:3860562/1‑150 (MQ=255)
                                                                                       gCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCa                                                               >  2:390207/1‑150 (MQ=255)
                                                                                        cAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCat                                                              >  1:2689385/1‑150 (MQ=255)
                                                                                         aaTTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCata                                                             <  1:1965441/150‑1 (MQ=255)
                                                                                              gTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGc                                                        <  1:1949443/150‑1 (MQ=255)
                                                                                              gTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGc                                                        <  1:565525/150‑1 (MQ=255)
                                                                                               tCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCa                                                       <  2:3717913/150‑1 (MQ=255)
                                                                                               tCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCa                                                       <  1:2396171/150‑1 (MQ=255)
                                                                                                cAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCaa                                                      <  2:1781146/150‑1 (MQ=255)
                                                                                                 aGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAAc                                                     >  2:1233919/1‑150 (MQ=255)
                                                                                                  gCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAAcc                                                    <  1:1902640/150‑1 (MQ=255)
                                                                                                     aTCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCAtt                                                 >  1:2965503/1‑150 (MQ=255)
                                                                                                     aTCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCAtt                                                 >  1:1513063/1‑150 (MQ=255)
                                                                                                      tCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCAttt                                                <  2:1803297/150‑1 (MQ=255)
                                                                                                      tCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCAttt                                                <  2:3752837/150‑1 (MQ=255)
                                                                                                       cGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCAtttt                                               <  2:1603217/150‑1 (MQ=255)
                                                                                                        gAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGATCTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTg                                              <  1:2266866/150‑1 (MQ=255)
                                                                                                        gAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTg                                              <  1:4557297/150‑1 (MQ=255)
                                                                                                            ttAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGa                                          >  2:3111271/1‑150 (MQ=255)
                                                                                                            ttAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAAACGTTACGGGAACTTAGCGCCAGACCGTCTTTGGAGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTAGCTGa                                          >  2:3111328/1‑150 (MQ=255)
                                                                                                                 aCTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAgc                                     >  2:894532/1‑150 (MQ=255)
                                                                                                                       tACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGt                               >  1:3340137/1‑150 (MQ=255)
                                                                                                                       tACAGACCAGGCGCAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCATCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGt                               >  1:4323339/1‑150 (MQ=255)
                                                                                                                        aCAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGtt                              <  1:1754157/150‑1 (MQ=255)
                                                                                                                        aCAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGtt                              >  1:2121996/1‑150 (MQ=255)
                                                                                                                        aCAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGtt                              <  1:3125334/150‑1 (MQ=255)
                                                                                                                                  cgcAATTTTGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATc                    >  2:713772/1‑150 (MQ=255)
                                                                                                                                    cAATTTTGCGTTGTACCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCtt                  >  2:1368814/1‑150 (MQ=255)
                                                                                                                                       ttttGCGTTGTTCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCttttt               <  1:1478859/150‑1 (MQ=255)
                                                                                                                                            cGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCg          <  1:2041777/150‑1 (MQ=255)
                                                                                                                                             gttgttCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGa         >  1:3241791/1‑150 (MQ=255)
                                                                                                                                             gttgttCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGa         >  2:1566316/1‑150 (MQ=255)
                                                                                                                                              ttgttCAGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGaa        <  2:2420304/150‑1 (MQ=255)
                                                                                                                                               tgttCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAg       <  2:1892647/150‑1 (MQ=255)
                                                                                                                                                gttCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGc      >  2:3801511/1‑150 (MQ=255)
                                                                                                                                                    cgccgTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGc  >  2:3628676/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCTGAATATCATCGGCGCGGAAGCCTTTTTCATTCAGTTCTTGCCCCGCAATGGTAATAATTTCATCGAGATCCCGTTCCCCAGCCTGCAATTTGTCAGCAATCGAACTTAAAACTTTGTACAGACCAGGCGCAATTTTGCGTTGTTCCGCCGTCAGATAACCGTTACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGC  >  CP009273/144550‑144847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑