Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,115,361 C→A 8.9% R367L (CGC→CTC)  cpsG ← phosphomannomutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,115,3610CA 8.9% 883.0 / 38.7 235R367L (CGC→CTC) cpsGphosphomannomutase
Reads supporting (aligned to +/- strand):  ref base C (104/110);  new base A (18/3);  total (122/113)
Fisher's exact test for biased strand distribution p-value = 1.08e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 241 total aligned reads displayed.

AGTCGGCAAAGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCAT  >  CP009273/2115212‑2115509
                                                                                                                                                     |                                                                                                                                                    
    ggCAAAGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTAc                                                                                                                                                  >  2:1281059/1‑150 (MQ=255)
      cAAAGGTCAAGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTAGCCGGAAACGCCGCCATCCGGTCGCGTACCa                                                                                                                                                >  1:2274432/1‑150 (MQ=255)
       aaaGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAg                                                                                                                                               >  1:4234384/1‑150 (MQ=255)
        aaGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGt                                                                                                                                              <  1:1993043/150‑1 (MQ=255)
           gTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCg                                                                                                                                           <  2:3612844/150‑1 (MQ=255)
            tCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGc                                                                                                                                          <  1:1205779/150‑1 (MQ=255)
              aTGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGccc                                                                                                                                        >  2:2355803/1‑150 (MQ=255)
                gCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAg                                                                                                                                      <  1:661639/150‑1 (MQ=255)
                  tGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCg                                                                                                                                    >  2:299882/1‑150 (MQ=255)
                  tGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCg                                                                                                                                    <  1:1485226/150‑1 (MQ=255)
                   gATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGt                                                                                                                                   >  2:3663501/1‑150 (MQ=255)
                     tGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGttt                                                                                                                                 <  2:290667/150‑1 (MQ=255)
                     tGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGttt                                                                                                                                 <  2:2650169/150‑1 (MQ=255)
                       ccATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTa                                                                                                                               >  1:798568/1‑150 (MQ=255)
                        cATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTAt                                                                                                                              >  2:3788378/1‑150 (MQ=255)
                          tCGGTGCGATCCACCGCCTGCGCCTCTCGGATAAATTGCTGTTCCACGCGTTTAATCGCCTCAACGGGTTGCGCCAGTTTTCTGTTGATATCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCt                                                                                                                            <  1:4619919/150‑1 (MQ=255)
                           cGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCtt                                                                                                                           >  1:3207332/1‑150 (MQ=255)
                            ggTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCttt                                                                                                                          >  2:964171/1‑150 (MQ=255)
                            ggTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCttt                                                                                                                          <  1:3726101/150‑1 (MQ=255)
                             gTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTc                                                                                                                         <  1:3071197/150‑1 (MQ=255)
                               gCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAg                                                                                                                       <  2:1978514/150‑1 (MQ=255)
                                cGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAgg                                                                                                                      <  1:3924560/150‑1 (MQ=255)
                                   tCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGcac                                                                                                                   <  1:2120001/150‑1 (MQ=255)
                                   tCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGcac                                                                                                                   <  1:4197635/150‑1 (MQ=255)
                                     cACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCacac                                                                                                                 >  1:2427347/1‑150 (MQ=255)
                                     cACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTGTTATCTTTCAGGCacac                                                                                                                 >  2:950140/1‑150 (MQ=255)
                                      aCCGCCAGCGCATCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACAcc                                                                                                                >  2:3347163/1‑150 (MQ=255)
                                        cGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAg                                                                                                              <  2:4487479/150‑1 (MQ=255)
                                           cAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTc                                                                                                           <  2:933339/150‑1 (MQ=255)
                                           cAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTc                                                                                                           <  1:3978033/150‑1 (MQ=255)
                                              cgcCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGc                                                                                                        <  2:3233905/150‑1 (MQ=255)
                                               gcCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCg                                                                                                       <  1:342024/150‑1 (MQ=255)
                                                    aCGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACcag                                                                                                  <  1:1845797/150‑1 (MQ=255)
                                                      ggCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACcagct                                                                                                <  2:250557/150‑2 (MQ=255)
                                                      ggCTAAAATGCTGTTCCAAGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTTATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACcagca                                                                                                <  2:4062307/150‑1 (MQ=255)
                                                        cTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCagcagc                                                                                              >  1:3056152/1‑150 (MQ=255)
                                                        cTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCagcagc                                                                                              >  1:2233896/1‑150 (MQ=255)
                                                         tAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGcc                                                                                             >  1:2832753/1‑150 (MQ=255)
                                                         tAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGcc                                                                                             <  2:1014869/150‑1 (MQ=255)
                                                          aaaaTGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCa                                                                                            >  1:4182103/1‑150 (MQ=255)
                                                          aaaaTGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAAAGCCGCCATCCGGTCGCGTACCAGTTAGCCCAGCGTTTTATCTTTCAGGCAAACCAGTTCGGCGACCAGCAGCCa                                                                                            >  2:4028777/1‑150 (MQ=255)
                                                           aaaTGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCAc                                                                                           >  1:1445740/1‑150 (MQ=255)
                                                            aaTGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACg                                                                                          <  1:1036998/150‑1 (MQ=255)
                                                             aTGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACgg                                                                                         >  1:4549832/1‑150 (MQ=255)
                                                              tGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACggg                                                                                        <  1:3173207/150‑1 (MQ=255)
                                                               gCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGa                                                                                       <  1:3791695/150‑1 (MQ=255)
                                                                 tGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATc                                                                                     <  2:1365816/150‑1 (MQ=255)
                                                                      cACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGcc                                                                                <  2:160866/150‑1 (MQ=255)
                                                                      cACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGcc                                                                                >  2:3804628/1‑150 (MQ=255)
                                                                         gcgGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCt                                                                             <  1:272066/150‑1 (MQ=255)
                                                                            gTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTc                                                                          <  2:4601285/150‑1 (MQ=255)
                                                                             ttAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCg                                                                         <  2:1099672/150‑1 (MQ=255)
                                                                                aTCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAg                                                                      >  2:13476/1‑150 (MQ=255)
                                                                                aTCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAg                                                                      >  1:1044837/1‑150 (MQ=255)
                                                                                 tCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGt                                                                     >  1:4520505/1‑150 (MQ=255)
                                                                                     cTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGc                                                                 >  1:1904062/1‑150 (MQ=255)
                                                                                        aaCGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGaa                                                              >  2:3683109/1‑150 (MQ=255)
                                                                                            ggTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCa                                                          >  2:2779280/1‑150 (MQ=255)
                                                                                             gTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCAc                                                         <  2:2740146/150‑1 (MQ=255)
                                                                                               tGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACgg                                                       >  1:341189/1‑150 (MQ=255)
                                                                                                 cgcCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGaa                                                     <  2:2561061/150‑1 (MQ=255)
                                                                                                 cgcCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGaa                                                     >  2:2060671/1‑150 (MQ=255)
                                                                                                 cgcCAGTGTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGaa                                                     <  2:2560383/150‑1 (MQ=255)
                                                                                                  gcCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAg                                                    <  1:979599/150‑1 (MQ=255)
                                                                                                  gcCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAg                                                    <  2:4306238/150‑1 (MQ=255)
                                                                                                  gcCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAg                                                    <  1:2997479/150‑1 (MQ=255)
                                                                                                   ccAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGt                                                   >  2:2245085/1‑150 (MQ=255)
                                                                                                    cAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTa                                                  >  2:1877194/1‑150 (MQ=255)
                                                                                                    cAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTa                                                  >  1:2353439/1‑150 (MQ=255)
                                                                                                     aGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTaa                                                 >  2:3224091/1‑150 (MQ=255)
                                                                                                     aGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTaa                                                 <  2:711666/150‑1 (MQ=255)
                                                                                                     aGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTaa                                                 >  2:3062642/1‑150 (MQ=255)
                                                                                                     aGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTaa                                                 >  2:743307/1‑150 (MQ=255)
                                                                                                           cTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAAtggtgg                                           <  2:2921654/150‑1 (MQ=255)
                                                                                                           cTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAAtggtgg                                           >  1:1319987/1‑150 (MQ=255)
                                                                                                                gATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCt                                      >  2:2439962/1‑150 (MQ=255)
                                                                                                                gATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCt                                      >  1:1973795/1‑150 (MQ=255)
                                                                                                                   ctcACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCAt                                   >  2:4419102/1‑150 (MQ=255)
                                                                                                                    tcACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCAtt                                  <  1:3749585/150‑1 (MQ=255)
                                                                                                                    tcACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCAtt                                  >  1:3548908/1‑150 (MQ=255)
                                                                                                                          cTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGcca                            >  2:1087914/1‑150 (MQ=255)
                                                                                                                          cTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGcca                            >  2:920383/1‑150 (MQ=255)
                                                                                                                           ttGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGccac                           <  1:4326645/150‑1 (MQ=255)
                                                                                                                            tGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGccacc                          <  2:365706/150‑1 (MQ=255)
                                                                                                                               cGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATa                       >  2:4440303/1‑150 (MQ=255)
                                                                                                                                ggAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAg                      >  2:1236186/1‑150 (MQ=255)
                                                                                                                                 gAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGa                     <  2:760464/150‑1 (MQ=255)
                                                                                                                                  aaaCGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTACGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGAt                    >  2:196806/1‑150 (MQ=255)
                                                                                                                                   aaCGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATg                   <  1:774856/150‑1 (MQ=255)
                                                                                                                                   aaCGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATATCGCCACCATAGATg                   <  2:1719432/150‑1 (MQ=255)
                                                                                                                                     cgccgcCATCCGGTCGCTTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGGAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAATTAATGGTGGGCGCTCTTTTCGCCACCATAGATGGc                 <  2:3904985/150‑1 (MQ=255)
                                                                                                                                     cgccgcCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGc                 >  1:3322810/1‑150 (MQ=255)
                                                                                                                                      gccgccATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCg                >  2:4404469/1‑150 (MQ=255)
                                                                                                                                      gccgccATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCg                >  1:4491179/1‑150 (MQ=255)
                                                                                                                                            aTCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTcc          <  2:3712554/150‑1 (MQ=255)
                                                                                                                                            aTCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTcc          <  1:3467403/150‑1 (MQ=255)
                                                                                                                                               cGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTg       <  2:3384902/150‑1 (MQ=255)
                                                                                                                                                ggTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTgc      >  2:4110753/1‑150 (MQ=255)
                                                                                                                                                   cgcgTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCa   <  2:2834781/150‑1 (MQ=255)
                                                                                                                                                    gcgTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCAt  >  2:1842038/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AGTCGGCAAAGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCAT  >  CP009273/2115212‑2115509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑