Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,194,695 T→A 100% L313Q (CTA→CAA)  yehI → DUF4132 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,194,6950TA96.8% 963.4 / 15.2 283L313Q (CTA→CAA) yehIDUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (4/5);  new base A (135/139);  total (139/144)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 283 total aligned reads displayed.

AAAACCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAGACA  >  CP009273/2194548‑2194841
                                                                                                                                                   |                                                                                                                                                  
aaaaCCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTAc                                                                                                                                                  <  2:822977/150‑1 (MQ=255)
aaaaCCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAAc                                                                                                                                                  >  2:3284560/1‑150 (MQ=255)
aaaaCCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAAc                                                                                                                                                  <  2:2949226/150‑1 (MQ=255)
 aaaCCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACt                                                                                                                                                 <  1:2920557/150‑1 (MQ=255)
 aaaCCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACt                                                                                                                                                 <  1:2668141/150‑1 (MQ=255)
  aaCCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACtg                                                                                                                                                >  2:1715765/1‑150 (MQ=255)
  aaCCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACtg                                                                                                                                                >  2:449464/1‑150 (MQ=255)
   aCCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACtgg                                                                                                                                               >  1:4607982/1‑150 (MQ=255)
        ttCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCt                                                                                                                                          <  2:1511750/150‑1 (MQ=255)
        ttCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCt                                                                                                                                          <  2:2034786/150‑1 (MQ=255)
        ttCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCt                                                                                                                                          >  2:3139346/1‑150 (MQ=255)
         tCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTg                                                                                                                                         >  2:4209048/1‑150 (MQ=255)
          cGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGa                                                                                                                                        >  1:471718/1‑150 (MQ=255)
          cGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGa                                                                                                                                        >  1:2375652/1‑150 (MQ=255)
           ggCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGAt                                                                                                                                       >  1:1490529/1‑150 (MQ=255)
            gCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATa                                                                                                                                      >  2:3348179/1‑150 (MQ=255)
             cTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATaa                                                                                                                                     >  1:3183777/1‑150 (MQ=255)
              tACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATaaa                                                                                                                                    >  2:1075500/1‑150 (MQ=255)
               aCGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATaaaa                                                                                                                                   <  1:680297/150‑1 (MQ=255)
                cgcgGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCAGTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATaaaaa                                                                                                                                  <  1:71144/150‑1 (MQ=255)
                  cgGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAaaaca                                                                                                                                <  2:4198425/150‑1 (MQ=255)
                  cgGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTAGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATaaaaaaa                                                                                                                                >  1:4526951/1‑148 (MQ=255)
                  cgGCCTGCGCCGCCATTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGACTCGCGATGTATGCCCATAAAGAAGATTGCTGCAGACTGCTGGTACAAAATAACCATCCGAAAGTCATCCGCACCTTCCAACTCGAGGCTAATAAaaaca                                                                                                                                >  2:4084991/1‑150 (MQ=255)
                   ggCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAaaacaa                                                                                                                               >  1:3851991/1‑150 (MQ=255)
                    gCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAaaacaaa                                                                                                                              >  1:2286780/1‑150 (MQ=255)
                       tGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAAccc                                                                                                                           <  1:2377376/150‑1 (MQ=255)
                        gcgcCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCa                                                                                                                          <  1:1661918/150‑1 (MQ=255)
                         cgccgcCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAg                                                                                                                         <  1:142088/150‑1 (MQ=255)
                         cgccgcCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAg                                                                                                                         >  2:498894/1‑150 (MQ=255)
                         cgccgcCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAg                                                                                                                         <  2:2630800/150‑1 (MQ=255)
                            cgccCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCt                                                                                                                      >  2:3962552/1‑150 (MQ=255)
                            cgccCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCt                                                                                                                      >  2:1331274/1‑150 (MQ=255)
                              cccTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGc                                                                                                                    >  1:3440078/1‑150 (MQ=255)
                               ccTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCa                                                                                                                   >  2:317187/1‑150 (MQ=255)
                                cTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCaa                                                                                                                  >  2:4569607/1‑150 (MQ=255)
                                  tttGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACg                                                                                                                <  2:309655/150‑1 (MQ=255)
                                  tttGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACg                                                                                                                <  1:2936113/150‑1 (MQ=255)
                                    tGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACgtg                                                                                                              >  1:4307107/1‑150 (MQ=255)
                                     gcgcGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACgtgt                                                                                                             <  1:2794076/150‑1 (MQ=255)
                                       gcgAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAg                                                                                                           <  1:2595817/150‑1 (MQ=255)
                                        cgAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGc                                                                                                          <  1:3334277/150‑1 (MQ=255)
                                         gAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGACATCCGCACCTTGCTACTGGAGGCTGATAAAAACAAACCCAGCCAGCAACGTGTAGCt                                                                                                         >  1:1817828/1‑150 (MQ=255)
                                            cAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTaaa                                                                                                      >  1:200085/1‑150 (MQ=255)
                                             aaGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAAt                                                                                                     >  1:1959267/1‑150 (MQ=255)
                                              aGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATa                                                                                                    <  1:458662/150‑1 (MQ=255)
                                              aGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATa                                                                                                    <  2:461469/150‑1 (MQ=255)
                                               ggACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATAc                                                                                                   <  2:3340004/150‑1 (MQ=255)
                                               ggACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATAc                                                                                                   >  1:410269/1‑150 (MQ=255)
                                               ggACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATAc                                                                                                   <  2:2667531/150‑1 (MQ=255)
                                                    ggCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATaa                                                                                              <  2:2229650/150‑1 (MQ=255)
                                                    ggCAGCCAGTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATaa                                                                                              <  2:1000176/150‑1 (MQ=255)
                                                       aGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATaaaaa                                                                                           >  1:994752/1‑150 (MQ=255)
                                                        gCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAAc                                                                                          >  1:111926/1‑150 (MQ=255)
                                                        gCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAAc                                                                                          <  1:3256776/150‑1 (MQ=255)
                                                         ccATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACt                                                                                         <  2:1100973/150‑1 (MQ=255)
                                                             tCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTccc                                                                                     <  1:449231/150‑1 (MQ=255)
                                                              ccGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTcccc                                                                                    <  1:1655790/150‑1 (MQ=255)
                                                                  gcCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATg                                                                                <  2:3820846/150‑1 (MQ=255)
                                                                  gcCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATg                                                                                <  2:3478695/150‑1 (MQ=255)
                                                                    cTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCg                                                                              <  2:4416055/150‑1 (MQ=255)
                                                                      cgcgATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGAc                                                                            <  2:130819/150‑1 (MQ=255)
                                                                      cgcgATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGAc                                                                            <  1:4129656/150‑1 (MQ=255)
                                                                        cgATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGc                                                                          >  1:1319491/1‑150 (MQ=255)
                                                                        cgATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGc                                                                          >  1:3058558/1‑150 (MQ=255)
                                                                          aTGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTc                                                                        <  1:2351929/150‑1 (MQ=255)
                                                                           tGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTcg                                                                       >  1:3152976/1‑150 (MQ=255)
                                                                              aTGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTcgccg                                                                    <  1:849518/150‑1 (MQ=255)
                                                                                gCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCa                                                                  >  2:2811512/1‑150 (MQ=255)
                                                                                  ccATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACt                                                                <  2:1916042/150‑1 (MQ=255)
                                                                                  ccATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACt                                                                <  1:3557122/150‑1 (MQ=255)
                                                                                   cATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTg                                                               >  2:1432551/1‑150 (MQ=255)
                                                                                    aTAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTgg                                                              <  1:614430/150‑1 (MQ=255)
                                                                                     tAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGc                                                             >  2:3199479/1‑150 (MQ=255)
                                                                                        agaagaATGCGGAAGGCTGCTGGTACACGTTAACCATCCGCAAGTCATCCGCACCTTGCAACAGGTGGCAGATAAAAAAAAAACCAGCCTGAAAAGGTTATCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCTCACTCGCAGa                                                          >  2:749612/1‑150 (MQ=255)
                                                                                           agaTTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGTAct                                                       <  1:2239531/150‑1 (MQ=255)
                                                                                             aTTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAActgc                                                     <  2:2603994/150‑1 (MQ=255)
                                                                                              ttGCGGCTGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACGGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAActgct                                                    <  1:2201843/150‑1 (MQ=255)
                                                                                                gCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTgg                                                  <  2:3640640/150‑1 (MQ=255)
                                                                                                     aGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTa                                             <  1:2886776/150‑1 (MQ=255)
                                                                                                     aGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTa                                             <  1:2027690/150‑1 (MQ=255)
                                                                                                            tgGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAac                                      <  2:2731211/150‑1 (MQ=255)
                                                                                                                aCAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAaccacc                                  >  1:2771265/1‑150 (MQ=255)
                                                                                                                 cAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAAccacca                                 <  1:2018554/150‑1 (MQ=255)
                                                                                                                    aTTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAtcc                              <  2:2788767/150‑1 (MQ=255)
                                                                                                                     ttAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgccc                             <  2:3183964/150‑1 (MQ=255)
                                                                                                                     ttAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgccc                             >  1:3825514/1‑150 (MQ=255)
                                                                                                                       aaCCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgcccgc                           <  2:3639102/150‑1 (MQ=255)
                                                                                                                        aCCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgcccgcc                          <  1:1539621/150‑1 (MQ=255)
                                                                                                                        aCCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgcccgcc                          >  2:2895393/1‑150 (MQ=255)
                                                                                                                         ccATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgcccgccc                         >  1:1052750/1‑150 (MQ=255)
                                                                                                                          cATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCt                        >  2:4173404/1‑150 (MQ=255)
                                                                                                                          cATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCt                        <  1:3770565/150‑1 (MQ=255)
                                                                                                                          cATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCt                        <  1:3864000/150‑1 (MQ=255)
                                                                                                                                cAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTAt                  <  2:2105193/150‑1 (MQ=255)
                                                                                                                                 aaGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATc                 >  2:1618901/1‑150 (MQ=255)
                                                                                                                                 aaGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATc                 >  2:3454487/1‑150 (MQ=255)
                                                                                                                                      aTCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTCGCTAAATACCAAAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAatc            >  2:1249616/1‑150 (MQ=255)
                                                                                                                                      aTCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAatc            <  1:441572/150‑1 (MQ=255)
                                                                                                                                      aTCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAatc            <  2:52449/150‑1 (MQ=255)
                                                                                                                                          gCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCg        <  1:648845/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAAACCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAGACA  >  CP009273/2194548‑2194841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑