Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,232,373 C→A 6.5% A132A (GCG→GCT)  mglA ← fused methyl‑galactoside transporter subunits of ABC superfamily: ATP‑binding components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,232,3730CA 6.5% 1239.8 / 36.5 323A132A (GCG→GCT) mglAfused methyl‑galactoside transporter subunits of ABC superfamily: ATP‑binding components
Reads supporting (aligned to +/- strand):  ref base C (125/177);  new base A (13/8);  total (138/185)
Fisher's exact test for biased strand distribution p-value = 7.23e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 329 total aligned reads displayed.

TCGGTTAACGAAGAAGTCGGTTCATCCATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTCCTGGTGTACCATCGAAATACCATTTTCCAGGGCTTCTTT  >  CP009273/2232224‑2232522
                                                                                                                                                     |                                                                                                                                                     
 cGGTTAACGAAGAAGTCGGTTCATCCATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCg                                                                                                                                                      >  2:1104996/1‑150 (MQ=255)
   gTTAACGAAGAAGTCGGTTCATCCATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCt                                                                                                                                                    >  1:2449887/1‑150 (MQ=255)
     tAACGAAGAAGTCGGTTCATCCATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCttt                                                                                                                                                  <  1:1247493/150‑1 (MQ=255)
     tAACGAAGAAGTCGGTTCATCCATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCttt                                                                                                                                                  >  2:4420652/1‑150 (MQ=255)
      aaCGAAGAAGTCGGTTCATCCATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTg                                                                                                                                                 >  2:2139678/1‑150 (MQ=255)
         gaagaaGTCGGTTCATCCATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGAGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGtt                                                                                                                                              <  2:2983974/150‑1 (MQ=255)
          aagaagTCGGTTCATCCATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGttt                                                                                                                                             <  1:1495903/150‑1 (MQ=255)
           agaagTCGGTTCATCCATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTc                                                                                                                                            >  1:3233179/1‑150 (MQ=255)
            gaagTCGGTTCATCCATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTcg                                                                                                                                           <  1:2805564/150‑1 (MQ=255)
             aagTCGGTTCATCCATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTcgc                                                                                                                                          >  2:1790553/1‑150 (MQ=255)
              agTCGGTTCATCCATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTcgcg                                                                                                                                         >  1:4575191/1‑150 (MQ=255)
                 cGGTTCATCCATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTa                                                                                                                                      <  2:3055610/150‑1 (MQ=255)
                  ggTTCATCCATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTAc                                                                                                                                     >  2:2917265/1‑150 (MQ=255)
                   gTTCATCCATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACa                                                                                                                                    >  1:1578552/1‑150 (MQ=255)
                   gTTCATCCATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACa                                                                                                                                    >  1:2043148/1‑150 (MQ=255)
                   gTTCATCCATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACa                                                                                                                                    <  1:3895908/150‑1 (MQ=255)
                      cATCCATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACAttt                                                                                                                                 <  1:732330/150‑1 (MQ=255)
                       aTCCATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACAtttt                                                                                                                                <  2:4085580/150‑1 (MQ=255)
                        tCCATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTg                                                                                                                               <  1:658630/150‑1 (MQ=255)
                        tCCATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTg                                                                                                                               <  1:1653029/150‑1 (MQ=255)
                        tCCATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTg                                                                                                                               <  1:4082651/150‑1 (MQ=255)
                          cATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTc                                                                                                                             <  1:3528852/150‑1 (MQ=255)
                             aaTCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTg                                                                                                                          >  1:4122141/1‑150 (MQ=255)
                                cacaATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATc                                                                                                                       <  1:852248/150‑1 (MQ=255)
                                cacaATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATc                                                                                                                       <  2:272913/150‑1 (MQ=255)
                                cacaATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATc                                                                                                                       <  1:3721870/150‑1 (MQ=255)
                                   aaTTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGAc                                                                                                                    >  1:3606233/1‑150 (MQ=255)
                                      tttCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACaaa                                                                                                                 <  2:3885370/150‑1 (MQ=255)
                                        tCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACa                                                                                                               >  2:536735/1‑150 (MQ=255)
                                         cgcgTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACAt                                                                                                              <  2:53514/150‑1 (MQ=255)
                                         cgcgTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACAt                                                                                                              <  1:3152198/150‑1 (MQ=255)
                                          gcgTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATg                                                                                                             <  2:188281/150‑1 (MQ=255)
                                            gTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATAGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGcc                                                                                                           >  1:110194/1‑150 (MQ=255)
                                               ataGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCttt                                                                                                        <  2:4482643/150‑1 (MQ=255)
                                                taGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTtg                                                                                                       >  1:1764339/1‑150 (MQ=255)
                                                   gAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTtggtg                                                                                                    <  1:2250222/150‑1 (MQ=255)
                                                   gAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTtggtg                                                                                                    >  1:4118735/1‑150 (MQ=255)
                                                    aaaaCGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATAGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTtggtgg                                                                                                   >  1:2811078/1‑150 (MQ=255)
                                                     aaaCGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCATGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTggg                                                                                                  >  1:3322388/1‑150 (MQ=255)
                                                     aaaCGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTggg                                                                                                  >  1:1817027/1‑150 (MQ=255)
                                                      aaCGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGa                                                                                                 <  2:4199799/150‑1 (MQ=255)
                                                          cTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATc                                                                                             <  1:1362492/150‑1 (MQ=255)
                                                          cTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATc                                                                                             >  1:4478350/1‑150 (MQ=255)
                                                            ttGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGc                                                                                           >  2:1320590/1‑150 (MQ=255)
                                                                cGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCa                                                                                       <  2:3623257/150‑1 (MQ=255)
                                                                cGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCa                                                                                       <  2:1156372/150‑1 (MQ=255)
                                                                cGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCa                                                                                       >  1:2184012/1‑150 (MQ=255)
                                                                      cGATCATCTTCATTTGAGAAACGGATAATGTGCAGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCcaca                                                                                 <  2:2075108/150‑1 (MQ=255)
                                                                       gATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACAt                                                                                >  1:2222972/1‑150 (MQ=255)
                                                                        atcatcTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATg                                                                               <  1:3236827/150‑1 (MQ=255)
                                                                        atcatcTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATAGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATg                                                                               >  1:2912277/1‑150 (MQ=255)
                                                                         tcatcTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGt                                                                              <  1:2402262/150‑1 (MQ=255)
                                                                         tcatcTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGt                                                                              <  1:670609/150‑1 (MQ=255)
                                                                           atcTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATAGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTa                                                                            >  2:2232635/1‑150 (MQ=255)
                                                                           atcTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATAGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTa                                                                            >  1:4027280/1‑150 (MQ=255)
                                                                             cTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATc                                                                          <  2:826277/150‑1 (MQ=255)
                                                                              tGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATcc                                                                         >  2:1917691/1‑150 (MQ=255)
                                                                               gCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCa                                                                        >  1:3056882/1‑150 (MQ=255)
                                                                                 aTTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATc                                                                      >  1:2776092/1‑150 (MQ=255)
                                                                                  tttGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCa                                                                     >  1:3544094/1‑150 (MQ=255)
                                                                                  tttGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCa                                                                     >  2:1612715/1‑150 (MQ=255)
                                                                                       gAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGaa                                                                >  1:117822/1‑150 (MQ=255)
                                                                                        aaaCGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAAc                                                               >  2:1282487/1‑150 (MQ=255)
                                                                                          aCGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGt                                                             >  1:414216/1‑150 (MQ=255)
                                                                                            ggATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTg                                                           >  2:2629949/1‑150 (MQ=255)
                                                                                                  tgtgCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATAc                                                     <  1:2601103/150‑1 (MQ=255)
                                                                                                  tgtgCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATAc                                                     >  1:1530364/1‑150 (MQ=255)
                                                                                                    tgCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCa                                                   <  1:2228875/150‑1 (MQ=255)
                                                                                                      ccGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAgg                                                 <  2:1094959/150‑1 (MQ=255)
                                                                                                          cgcgcgCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTa                                             >  1:1025177/1‑150 (MQ=255)
                                                                                                          cgcgcgCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTa                                             <  2:3101297/150‑1 (MQ=255)
                                                                                                          cgcgcgCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTa                                             >  2:608588/1‑150 (MQ=255)
                                                                                                            cgcgcACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAAc                                           >  2:432047/1‑150 (MQ=255)
                                                                                                             gcgcACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACt                                          <  2:2941278/150‑1 (MQ=255)
                                                                                                             gcgcACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACt                                          <  2:3905469/150‑1 (MQ=255)
                                                                                                              cgcACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTc                                         <  1:4266559/150‑1 (MQ=255)
                                                                                                               gcACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTcc                                        <  1:461153/150‑1 (MQ=255)
                                                                                                               gcACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTcc                                        >  2:28455/1‑150 (MQ=255)
                                                                                                               gcACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTcc                                        >  2:3287873/1‑150 (MQ=255)
                                                                                                                cACGCGGATCGATATCAATATCCAGTTCGTCAAAAATAGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTCCt                                       <  1:823110/150‑1 (MQ=255)
                                                                                                                 aCGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTCCTg                                      <  2:1280323/150‑1 (MQ=255)
                                                                                                                 aCGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTCCTg                                      <  1:1951293/150‑1 (MQ=255)
                                                                                                                    cgGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTCCTGgtg                                   >  2:3118933/1‑150 (MQ=255)
                                                                                                                     ggATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTCCTGgtgt                                  >  2:2456927/1‑150 (MQ=255)
                                                                                                                        tCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTCCTGGTGTAcc                               <  1:4351239/150‑1 (MQ=255)
                                                                                                                         cGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTCCTGGTGTACCa                              <  1:2976855/150‑1 (MQ=255)
                                                                                                                         cGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTCCTGGTGTACCa                              <  1:976970/150‑1 (MQ=255)
                                                                                                                              tCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTCCTGGTGTACCATCGaa                         <  2:840801/150‑1 (MQ=255)
                                                                                                                                     ccAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTCCTGGTGTACCATCGAAATACCAt                  <  2:3233346/150‑1 (MQ=255)
                                                                                                                                     ccAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTCCTGGTGTACCATCGAAATACCAt                  >  2:3036591/1‑150 (MQ=255)
                                                                                                                                      cAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTCCTGGTGTACCATCGAAATACCAtt                 <  2:2781402/150‑1 (MQ=255)
                                                                                                                                      cAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTCCTGGTGTACCATCGAAATACCAtt                 <  2:4483280/150‑1 (MQ=255)
                                                                                                                                      cAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTCCTGGTGTACCATCGAAATACCAtt                 <  1:2693509/150‑1 (MQ=255)
                                                                                                                                       aGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTCCTGGTGTACCATCGAAATACCAttt                >  2:3920332/1‑150 (MQ=255)
                                                                                                                                              cAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTCCTGGTGTACCATCGAAATACCATTTTCCAggg         >  1:1978773/1‑150 (MQ=255)
                                                                                                                                               aaaaaTCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTCCTGGTGTACCATCGAAATACCATTTTCCAGGGc        >  1:798/1‑150 (MQ=255)
                                                                                                                                                    tCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTCCTGGTGTACCATCGAAATACCATTTTCCAGGGcttctt   <  2:3571544/150‑1 (MQ=255)
                                                                                                                                                    tCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTCCTGGTGTACCATCGAAATACCATTTTCCAGGGcttctt   >  1:4321891/1‑150 (MQ=255)
                                                                                                                                                    tCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTCCTGGTGTACCATCGAAATACCATTTTCCAGGGcttctt   >  2:668981/1‑150 (MQ=255)
                                                                                                                                                     cGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTCCTGGTGTACCATCGAAATACCATTTTCCAGGGCTTCttt  <  1:701880/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCGGTTAACGAAGAAGTCGGTTCATCCATAATCACAATTTTCGCGTTATAGGAAAACGCTTTGGCGATTTCGATCATCTGCATTTGCGAAACGGATAATGTGCCGACGCGCGCACGCGGATCGATATCAATATCCAGTTCGTCAAAAATCGCTTTGGTTTCGCGGTACATTTTGTCCTGATCGACAAACATGCCTTTGGTGGGATATCGCCCCAGCCACATGTTATCCATCACCGAACGTTGTAATACCAGGTTTAACTCCTGGTGTACCATCGAAATACCATTTTCCAGGGCTTCTTT  >  CP009273/2232224‑2232522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑