Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,233,243 T→G 5.2% K195N (AAA→AAC)  mglB ← methyl‑galactoside transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,233,2430TG 5.2% 990.0 / 40.2 319K195N (AAA→AAC) mglBmethyl‑galactoside transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (135/167);  new base G (7/10);  total (142/177)
Fisher's exact test for biased strand distribution p-value = 8.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 324 total aligned reads displayed.

AGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATCCCAACCCTGATTCGCCGCCCAGTGTTTAGCAAT  >  CP009273/2233094‑2233392
                                                                                                                                                     |                                                                                                                                                     
     ttCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGAt                                                                                                                                                  <  1:2561990/150‑1 (MQ=255)
       cAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGc                                                                                                                                                >  2:52076/1‑150 (MQ=255)
       cAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGc                                                                                                                                                >  2:4447029/1‑150 (MQ=255)
        aaCCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGcc                                                                                                                                               <  1:303233/150‑1 (MQ=255)
        aaCCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGcc                                                                                                                                               >  2:4634312/1‑150 (MQ=255)
          ccGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCtt                                                                                                                                             <  2:3160091/150‑1 (MQ=255)
           cgcgCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCttt                                                                                                                                            <  2:4500244/150‑1 (MQ=255)
              gcCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATc                                                                                                                                         <  2:175759/150‑1 (MQ=255)
              gcCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATc                                                                                                                                         <  2:175566/150‑1 (MQ=255)
               cccATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTGTTGATGCCTTTATCg                                                                                                                                        <  2:852402/150‑1 (MQ=255)
                ccATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGt                                                                                                                                       >  2:2478770/1‑150 (MQ=255)
                ccATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGt                                                                                                                                       >  2:3361762/1‑150 (MQ=255)
                ccATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGt                                                                                                                                       >  1:2696629/1‑150 (MQ=255)
                 cATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGtt                                                                                                                                      <  2:1140013/150‑1 (MQ=255)
                 cATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGtt                                                                                                                                      >  2:1115469/1‑150 (MQ=255)
                   ttGCCATCGCATCGTTGTTGGCGATAACCAGTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCa                                                                                                                                    >  1:4290255/1‑150 (MQ=255)
                    tGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCaa                                                                                                                                   >  1:1716144/1‑150 (MQ=255)
                    tGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCaa                                                                                                                                   <  2:3200214/150‑1 (MQ=255)
                    tGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCaa                                                                                                                                   >  1:1623963/1‑150 (MQ=255)
                          cgcatcgTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCttt                                                                                                                             <  1:3715411/150‑1 (MQ=255)
                             atcgTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAAt                                                                                                                          >  1:2831335/1‑150 (MQ=255)
                             atcgTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAAt                                                                                                                          >  1:3380127/1‑150 (MQ=255)
                                gttgttGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCAc                                                                                                                       >  1:4245148/1‑150 (MQ=255)
                                  tgttgGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGACTTTATCGTTCAATTCTTTAATCACGt                                                                                                                     >  2:4351723/1‑150 (MQ=255)
                                   gttgGCGATAACCACTTCGATTTTGTTGTCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTa                                                                                                                    <  2:3837973/150‑1 (MQ=255)
                                     tgGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAg                                                                                                                  >  1:3050258/1‑150 (MQ=255)
                                      ggCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGt                                                                                                                 <  2:4536285/150‑1 (MQ=255)
                                       gCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTg                                                                                                                <  2:2857066/150‑1 (MQ=255)
                                       gCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTg                                                                                                                <  2:3073089/150‑1 (MQ=255)
                                        cGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTgg                                                                                                               <  1:2018630/150‑1 (MQ=255)
                                        cGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTgg                                                                                                               <  2:2140633/150‑1 (MQ=255)
                                         gATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGt                                                                                                              >  2:2692050/1‑150 (MQ=255)
                                         gATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCAAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGt                                                                                                              >  2:2118344/1‑150 (MQ=255)
                                          aTAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTa                                                                                                             >  1:330123/1‑150 (MQ=255)
                                               cACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGc                                                                                                        <  2:3743415/150‑1 (MQ=255)
                                                aCTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCt                                                                                                       <  1:1043566/150‑1 (MQ=255)
                                                aCTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCt                                                                                                       >  2:2539413/1‑150 (MQ=255)
                                                   tCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCt                                                                                                    <  1:3627306/150‑1 (MQ=255)
                                                   tCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCt                                                                                                    <  1:816308/150‑1 (MQ=255)
                                                      aTTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCa                                                                                                 >  1:1345496/1‑150 (MQ=255)
                                                      aTTTTGTTGGCGGTCGGGCCAGACAGCCAGGCGTCAATCTTATCTTTCGCCTGAGCGGTGTCACACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCGTTATCGTACAATTCTTTAATCACGTAAGTGGAACGTGCTTCTGCa                                                                                                 >  2:3445853/1‑150 (MQ=255)
                                                       ttttGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTACCTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCAt                                                                                                <  2:4303994/150‑1 (MQ=255)
                                                         ttgttgGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTGTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATcc                                                                                              <  2:3608990/150‑1 (MQ=255)
                                                             tgGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGTGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGAt                                                                                          <  1:1260998/150‑1 (MQ=255)
                                                             tgGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGAt                                                                                          <  2:1538084/150‑1 (MQ=255)
                                                             tgGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGAt                                                                                          <  2:255181/150‑1 (MQ=255)
                                                             tgGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCGTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGAt                                                                                          >  1:706701/1‑150 (MQ=255)
                                                               gCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATgg                                                                                        <  2:1620520/150‑1 (MQ=255)
                                                                    cGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCgg                                                                                   >  2:3759133/1‑150 (MQ=255)
                                                                    cGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCgg                                                                                   >  1:1527962/1‑150 (MQ=255)
                                                                     gggCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTGTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGt                                                                                  >  1:4411886/1‑150 (MQ=255)
                                                                         cAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCAc                                                                              <  2:4163166/150‑1 (MQ=255)
                                                                              aGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTc                                                                         >  2:505556/1‑150 (MQ=255)
                                                                              aGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTc                                                                         >  2:1573228/1‑150 (MQ=255)
                                                                               gcgaGGCTTACATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTGTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTca                                                                        <  2:3902311/147‑1 (MQ=255)
                                                                                     cGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTCAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTa                                                                  <  2:2036040/150‑1 (MQ=255)
                                                                                     cGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTa                                                                  <  2:1961686/150‑1 (MQ=255)
                                                                                     cGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTa                                                                  >  1:3746321/1‑150 (MQ=255)
                                                                                      gTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTAc                                                                 <  1:296499/150‑1 (MQ=255)
                                                                                      gTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTAc                                                                 <  1:1538677/150‑1 (MQ=255)
                                                                                       tCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACg                                                                <  2:2224771/150‑1 (MQ=255)
                                                                                       tCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACg                                                                >  1:1915851/1‑150 (MQ=255)
                                                                                           tcttatcttTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACt                                                            >  1:4516429/1‑150 (MQ=255)
                                                                                             ttatcttTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGa                                                          <  1:678004/150‑1 (MQ=255)
                                                                                                 cttTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCt                                                      <  2:2524430/150‑1 (MQ=255)
                                                                                                 cttTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCt                                                      >  1:896624/1‑150 (MQ=255)
                                                                                                 cttTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCt                                                      >  2:277411/1‑150 (MQ=255)
                                                                                                   ttCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGa                                                    <  1:208308/150‑1 (MQ=255)
                                                                                                     cGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGAAGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGAcc                                                  <  1:3862921/150‑1 (MQ=255)
                                                                                                         tGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCt                                              >  2:1888849/1‑150 (MQ=255)
                                                                                                              ggTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGtt                                         <  1:3244029/150‑1 (MQ=255)
                                                                                                              ggTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGtt                                         <  2:3510471/150‑1 (MQ=255)
                                                                                                                tgtCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCa                                       <  2:4074246/150‑1 (MQ=255)
                                                                                                                 gtCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAg                                      <  2:3050124/150‑1 (MQ=255)
                                                                                                                     cacaTTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATcc                                  >  1:2204949/1‑150 (MQ=255)
                                                                                                                      acaTTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATccc                                 <  1:2351474/150‑1 (MQ=255)
                                                                                                                      acaTTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATccc                                 <  1:3361762/150‑1 (MQ=255)
                                                                                                                      acaTTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATccc                                 >  2:2398692/1‑150 (MQ=255)
                                                                                                                      acaTTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTACAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATccc                                 >  1:3926228/1‑150 (MQ=255)
                                                                                                                       caTTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATCCCa                                >  1:2622591/1‑150 (MQ=255)
                                                                                                                           gCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATCCCAAccc                            >  1:46392/1‑150 (MQ=255)
                                                                                                                              gTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATCCCAACCCTGa                         <  2:714067/150‑1 (MQ=255)
                                                                                                                              gTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATCCCAACCCTGa                         >  2:580383/1‑150 (MQ=255)
                                                                                                                                aTCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATCCCAACCCTGAtt                       <  2:2269230/150‑1 (MQ=255)
                                                                                                                                 tCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATCCCAACCCTGATTc                      >  1:3963101/1‑150 (MQ=255)
                                                                                                                                 tCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATCCCAACCCTGATTc                      <  2:2873665/150‑1 (MQ=255)
                                                                                                                                 tCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATCCCAACCCTGATTc                      <  1:2599008/150‑1 (MQ=255)
                                                                                                                                 tCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATCCCAACCCTGATTc                      <  2:1300734/150‑1 (MQ=255)
                                                                                                                                   taactgtaactgTTCAGTGTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATCCCAACCCTGATTcgc                    >  1:4047476/1‑150 (MQ=255)
                                                                                                                                     actgtaactgtTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATCCCAACCCTGATTcgccg                  <  1:2408984/150‑1 (MQ=255)
                                                                                                                                      ctgtaactgtTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATCCCAACCCTGATTcgccgc                 >  2:4608202/1‑150 (MQ=255)
                                                                                                                                        gtaactgtTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATCCCAACCCTGATTCGCCGccc               <  2:4097359/150‑1 (MQ=255)
                                                                                                                                          aactgtTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATCCCAACCCTGATTCGCCGCCCAg             >  1:1122019/1‑150 (MQ=255)
                                                                                                                                          aactgtTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATCCCAACCCTGATTCGCCGCCCAg             >  2:2938543/1‑150 (MQ=255)
                                                                                                                                            ctgtTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATCCCAACCCTGATTCGCCGCCCAgtg           <  1:1842029/150‑1 (MQ=255)
                                                                                                                                             tgtTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATCCCAACCCTGATTCGCCGCCCAgtgt          >  2:2622467/1‑150 (MQ=255)
                                                                                                                                               ttCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATCCCAACCCTGATTCGCCGCCCAGTGttt        <  1:51995/150‑1 (MQ=255)
                                                                                                                                                tCAGTGTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATCCCAACCCTGATTCGCCGCCCAGTGTTTa       >  2:800188/1‑150 (MQ=255)
                                                                                                                                                  aGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATCCCAACCCTGATTCGCCGCCCAGTGTTTAGc     <  1:1445797/150‑1 (MQ=255)
                                                                                                                                                   gTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATCCCAACCCTGATTCGCCGCCCAGTGTTTAGCa    <  1:1499779/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTGAGCGGTGTCCCACATTGCGGTATCTAACTGTAACTGTTCAGTTTTGATGCCTTTATCGTTCAATTCTTTAATCACGTAAGTGGTACGTGCTTCTGCATCCGGATGGCCCGGTTCACCTTTCAGCAGTACGAACTGAATCTGACCGTCTTTGTTCAGATCCCAACCCTGATTCGCCGCCCAGTGTTTAGCAAT  >  CP009273/2233094‑2233392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑