Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,246,428 C→A 10.7% T399T (ACG→ACT)  nupX ← nucleoside permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,246,4280CA10.7% 1169.0 / 70.8 318T399T (ACG→ACT) nupXnucleoside permease
Reads supporting (aligned to +/- strand):  ref base C (162/122);  new base A (20/14);  total (182/136)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 323 total aligned reads displayed.

GATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGC  >  CP009273/2246279‑2246577
                                                                                                                                                     |                                                                                                                                                     
 aTGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCg                                                                                                                                                      <  1:2604090/150‑1 (MQ=255)
   gCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTc                                                                                                                                                    <  1:3239306/150‑1 (MQ=255)
       cGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTcgccg                                                                                                                                                <  1:4387302/150‑1 (MQ=255)
         cTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTcgccgcc                                                                                                                                              <  2:4484990/150‑1 (MQ=255)
         cTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTcgccgcc                                                                                                                                              <  1:2826759/150‑1 (MQ=255)
            aaCGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCACTGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCgccgccgcc                                                                                                                                           >  1:3098853/1‑150 (MQ=255)
             tcgcgTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCa                                                                                                                                          <  1:1307683/149‑1 (MQ=255)
             aCGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAACTGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCa                                                                                                                                          <  2:877594/150‑1 (MQ=255)
             aCGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCa                                                                                                                                          <  2:4553116/150‑1 (MQ=255)
             aCGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCa                                                                                                                                          <  1:2714013/150‑1 (MQ=255)
              cgcgTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAg                                                                                                                                         >  2:651155/1‑150 (MQ=255)
                cgTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAgcg                                                                                                                                       >  2:4642005/1‑150 (MQ=255)
                cgTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAgcg                                                                                                                                       >  2:484060/1‑150 (MQ=255)
                 gTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAgcgc                                                                                                                                      >  2:629458/1‑150 (MQ=255)
                   cTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGccc                                                                                                                                    <  1:533153/150‑1 (MQ=255)
                      aTCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTa                                                                                                                                 >  2:1178621/1‑150 (MQ=255)
                      aTCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTa                                                                                                                                 >  2:2207619/1‑150 (MQ=255)
                         cGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAAc                                                                                                                              >  2:1437378/1‑150 (MQ=255)
                          ggCCTACAAATCAGTCAATTTCAGCAAGTAAATTTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCTCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAAcc                                                                                                                             <  1:789821/150‑1 (MQ=255)
                          ggCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAAcc                                                                                                                             <  2:3634813/150‑1 (MQ=255)
                           gCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCa                                                                                                                            >  1:3589428/1‑150 (MQ=255)
                           gCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAACCa                                                                                                                            <  1:1690573/150‑1 (MQ=255)
                            ccTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCaa                                                                                                                           >  2:310571/1‑150 (MQ=255)
                             cTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAg                                                                                                                          >  2:4248643/1‑150 (MQ=255)
                                 aaaTCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTgg                                                                                                                      >  2:3260580/1‑150 (MQ=255)
                                 aaaTCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTgg                                                                                                                      >  2:1753323/1‑150 (MQ=255)
                                  aaTCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTggg                                                                                                                     <  2:2384691/150‑1 (MQ=255)
                                     cAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGa                                                                                                                  >  1:1516470/1‑150 (MQ=255)
                                      aGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGAt                                                                                                                 >  1:4549768/1‑150 (MQ=255)
                                      aGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGAt                                                                                                                 >  1:4549830/1‑150 (MQ=255)
                                        gCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGAttt                                                                                                               <  2:575106/150‑1 (MQ=255)
                                         cAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTc                                                                                                              >  2:593387/1‑150 (MQ=255)
                                          aaTTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTcc                                                                                                             <  2:2096794/150‑1 (MQ=255)
                                          aaTTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTcc                                                                                                             >  1:2196368/1‑150 (MQ=255)
                                             ttCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGgc                                                                                                          >  2:3188534/1‑150 (MQ=255)
                                              tCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGgcg                                                                                                         <  2:2366585/150‑1 (MQ=255)
                                                  cAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACg                                                                                                     <  2:4589311/150‑1 (MQ=255)
                                                   aaGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACgg                                                                                                    >  1:729509/1‑150 (MQ=255)
                                                     gTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGgtg                                                                                                  <  2:404730/150‑1 (MQ=255)
                                                      tAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGgtgt                                                                                                 >  1:1366499/1‑150 (MQ=255)
                                                      tAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGgtgt                                                                                                 >  1:1589920/1‑150 (MQ=255)
                                                        aaTGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTgg                                                                                               >  1:2523736/1‑150 (MQ=255)
                                                         aTGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGgc                                                                                              >  1:3274701/1‑150 (MQ=255)
                                                         aTGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGgc                                                                                              >  1:1645252/1‑150 (MQ=255)
                                                              ggCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAAc                                                                                         >  2:3019809/1‑150 (MQ=255)
                                                              ggCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAAc                                                                                         <  2:4100983/150‑1 (MQ=255)
                                                               gCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAAcc                                                                                        <  1:3891218/150‑1 (MQ=255)
                                                                ccTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCg                                                                                       <  2:1487438/150‑1 (MQ=255)
                                                                  tGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCa                                                                                     >  1:2923648/1‑150 (MQ=255)
                                                                  tGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCa                                                                                     <  2:112581/150‑1 (MQ=255)
                                                                  tGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCa                                                                                     <  2:3802847/150‑1 (MQ=255)
                                                                    aTAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGa                                                                                   >  1:1527830/1‑150 (MQ=255)
                                                                    aTAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGa                                                                                   >  2:4615978/1‑150 (MQ=255)
                                                                     tAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGaa                                                                                  >  1:966171/1‑150 (MQ=255)
                                                                     tAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGaa                                                                                  >  2:1983196/1‑150 (MQ=255)
                                                                       aGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGaaaa                                                                                >  1:3518123/1‑150 (MQ=255)
                                                                        gCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAAc                                                                               <  1:4191210/150‑1 (MQ=255)
                                                                         cTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACg                                                                              >  2:2522886/1‑150 (MQ=255)
                                                                          ttGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGc                                                                             >  1:1101612/1‑150 (MQ=255)
                                                                           tGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGcc                                                                            <  2:3684421/150‑1 (MQ=255)
                                                                              gcATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGccccc                                                                         >  2:845763/1‑150 (MQ=255)
                                                                              gcATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGccccc                                                                         >  1:1472559/1‑150 (MQ=255)
                                                                                aTCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCccac                                                                       >  2:3391899/1‑150 (MQ=255)
                                                                                  cAGGCACTGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCccacca                                                                     >  2:3930896/1‑150 (MQ=255)
                                                                                    ggCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCccaccacc                                                                   >  2:3350530/1‑150 (MQ=255)
                                                                                      cAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCcaccaccac                                                                 >  2:3194911/1‑150 (MQ=255)
                                                                                       aaTGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCaccaccacc                                                                >  2:3521685/1‑150 (MQ=255)
                                                                                       aaTGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCaccaccacc                                                                >  2:2417284/1‑150 (MQ=255)
                                                                                       aaTGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCaccaccacc                                                                >  1:1889016/1‑150 (MQ=255)
                                                                                         tGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCAcccc                                                              <  1:197055/150‑1 (MQ=255)
                                                                                            cacTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGAt                                                           >  2:1856822/1‑150 (MQ=255)
                                                                                            cacTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGAt                                                           <  1:2352898/150‑1 (MQ=255)
                                                                                            cacTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGAt                                                           <  1:4079000/150‑1 (MQ=255)
                                                                                             acTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATa                                                          <  2:2763666/150‑1 (MQ=255)
                                                                                             acTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATa                                                          <  2:1332003/150‑1 (MQ=255)
                                                                                             acTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATa                                                          <  2:2303271/150‑1 (MQ=255)
                                                                                               tCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGa                                                        <  1:2557703/150‑1 (MQ=255)
                                                                                                cAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGaa                                                       >  1:4310384/1‑150 (MQ=255)
                                                                                                   gCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTTGTAAGAGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCa                                                    <  2:752595/150‑1 (MQ=255)
                                                                                                   gCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCa                                                    <  1:4643313/150‑1 (MQ=255)
                                                                                                     tAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCaaa                                                  >  1:1559380/1‑150 (MQ=255)
                                                                                                         ccAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGtaa                                              >  1:1275881/1‑148 (MQ=255)
                                                                                                         ccAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTa                                              >  1:490648/1‑150 (MQ=255)
                                                                                                             tAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGa                                          <  1:1738712/150‑1 (MQ=255)
                                                                                                              aaaGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGaa                                         >  2:808612/1‑150 (MQ=255)
                                                                                                              aaaGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGaa                                         <  2:1061312/150‑1 (MQ=255)
                                                                                                                    cccGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGca                                   <  1:2517508/150‑1 (MQ=255)
                                                                                                                           aTGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAAcgcg                            <  2:4298141/150‑1 (MQ=255)
                                                                                                                             ggTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGaa                          >  1:1189419/1‑150 (MQ=255)
                                                                                                                               tCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAgg                        >  1:3943279/1‑150 (MQ=255)
                                                                                                                                cGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGa                       >  2:2930915/1‑150 (MQ=255)
                                                                                                                                 gCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGaa                      <  2:1801013/150‑1 (MQ=255)
                                                                                                                                 gCACTCATCAAGTTGGAAAGAGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGaa                      <  1:2747546/150‑1 (MQ=255)
                                                                                                                                  cACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGaaa                     >  1:3711853/1‑150 (MQ=255)
                                                                                                                                           aGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCca            <  2:1589920/150‑1 (MQ=255)
                                                                                                                                            gTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCcac           <  1:346156/150‑1 (MQ=255)
                                                                                                                                            gTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCcac           >  1:1994741/1‑150 (MQ=255)
                                                                                                                                                  aaGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGtttt     >  1:3176310/1‑150 (MQ=255)
                                                                                                                                                   aGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTa    >  1:1350796/1‑150 (MQ=255)
                                                                                                                                                     cGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGc  >  1:3502025/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCCAGCGCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGC  >  CP009273/2246279‑2246577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑