Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,287,028 C→A 40.9% T452T (ACG→ACT)  ccmF ← heme lyase, CcmF subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,287,0280CA40.9% 276.6 / 275.1 240T452T (ACG→ACT) ccmFheme lyase, CcmF subunit
Reads supporting (aligned to +/- strand):  ref base C (64/78);  new base A (43/55);  total (107/133)
Fisher's exact test for biased strand distribution p-value = 8.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 243 total aligned reads displayed.

GCAATGCCAACAATTGTCACTGCCAGCCCAAGGTGAGCCGCCACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAGCGCACCAGAGGACCGACACCAAGCAGTAGC  >  CP009273/2286879‑2287175
                                                                                                                                                     |                                                                                                                                                   
gCAATGCCAACAATTGTCACTGCCAGCCCAAGGTGAGCCGCCACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCAcc                                                                                                                                                     >  2:875319/1‑150 (MQ=255)
gCAATGCCAACAATTGGCACTGCCAGCCCAAGGTGAGCCGCCACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCAcc                                                                                                                                                     >  2:875316/1‑150 (MQ=255)
  aaTGCCAACAATTGTCACTGCCAGCCCAAGGTGAGCCGCCACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGt                                                                                                                                                   <  2:1694918/150‑1 (MQ=255)
    tGCCAACAATTGTCACTGCCAGCCCAAGGTGAGCCGCCACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCa                                                                                                                                                 <  1:338801/150‑1 (MQ=255)
      ccAACAATTGTCACTGCCAGCCCAAGGTGAGCCGCCACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATc                                                                                                                                               <  1:840008/150‑1 (MQ=255)
      ccAACAATTGTCACTGCCAGCCCAAGGTGAGCCGCCACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATc                                                                                                                                               >  2:2193984/1‑150 (MQ=255)
        aacaaTTGTCACTGCCAGCCCAAGGTGAGCCGCCACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGc                                                                                                                                             >  2:891741/1‑150 (MQ=255)
          caaTTGTCACTGCCAGCCCAAGGTGAGCCGCCACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCca                                                                                                                                           >  2:1132386/1‑150 (MQ=255)
          caaTTGTCACTGCCAGCCCAAGGTGAGCCGCCACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCca                                                                                                                                           >  1:2514670/1‑150 (MQ=255)
            aTTGTCACTGCCAGCCCAAGGTGAGCCGCCACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCTATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCcaca                                                                                                                                         >  2:129487/1‑150 (MQ=255)
              tGTCACTGCCAGCCCAAGGTGAGCCGCCACCATCCCCGAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAAc                                                                                                                                       >  1:1932996/1‑150 (MQ=255)
                 cACTGCCAGCCCAAGGTGAGCCGCCACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACttt                                                                                                                                    <  1:3580385/150‑1 (MQ=255)
                      ccAGCCCAAGGTGAGCCGCCACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCttt                                                                                                                               <  2:1484762/150‑1 (MQ=255)
                      ccAGCCCAAGGTGAGCCGCCACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCttt                                                                                                                               >  1:3785734/1‑150 (MQ=255)
                       cAGCCCAAGGTGAGCCGCTACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTc                                                                                                                              <  2:2106442/150‑1 (MQ=255)
                       cAGCCCAAGGTGAGCCGCCACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTc                                                                                                                              >  1:1972595/1‑150 (MQ=255)
                        aGCCCAAGGTGAGCCGCCACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCg                                                                                                                             <  1:1885369/150‑1 (MQ=255)
                        aGCCCAAGGTGAGCCGCCACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGCCATCGCCACAACTTTGCTTTCg                                                                                                                             <  1:4305342/150‑1 (MQ=255)
                             aaGGTGAGCCGCCACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAg                                                                                                                        >  2:3379875/1‑150 (MQ=255)
                             aaGGTGAGCCGCCACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAg                                                                                                                        >  2:1107745/1‑150 (MQ=255)
                                  gAGCCGCCACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACg                                                                                                                   <  2:2513778/150‑1 (MQ=255)
                                    gccgccACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGc                                                                                                                 >  1:3386741/1‑150 (MQ=255)
                                      cgccACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCaa                                                                                                               >  1:707942/1‑150 (MQ=255)
                                      cgccACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCaa                                                                                                               <  2:2376557/150‑1 (MQ=255)
                                        ccaccaTCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAAca                                                                                                             >  1:1119560/1‑150 (MQ=255)
                                        ccaccaTCCCCCAATAACTGAAGGTGGTGTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAAca                                                                                                             <  2:3050519/150‑1 (MQ=255)
                                          accaTCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAAcagc                                                                                                           >  1:1421819/1‑150 (MQ=255)
                                              tCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCg                                                                                                       >  2:1169345/1‑150 (MQ=255)
                                                      tAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCAcc                                                                                               >  2:1888365/1‑150 (MQ=255)
                                                        aCTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAg                                                                                             >  2:556054/1‑150 (MQ=255)
                                                        aCTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAg                                                                                             <  1:2397772/150‑1 (MQ=255)
                                                         cTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGc                                                                                            <  1:3457975/150‑1 (MQ=255)
                                                           gAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGt                                                                                          <  2:3617236/150‑1 (MQ=255)
                                                           gAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGt                                                                                          <  2:3790146/150‑1 (MQ=255)
                                                            aaGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTa                                                                                         <  1:3281218/150‑1 (MQ=255)
                                                             aGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTag                                                                                        >  1:145432/1‑150 (MQ=255)
                                                             aGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTag                                                                                        <  1:4617806/150‑1 (MQ=255)
                                                              ggtggtTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTaga                                                                                       <  2:1363339/150‑1 (MQ=255)
                                                                tggtTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAgaga                                                                                     >  2:2364356/1‑150 (MQ=255)
                                                                tggtTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAgaga                                                                                     <  2:1932996/150‑1 (MQ=255)
                                                                   ttttCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGa                                                                                  <  1:4066159/150‑1 (MQ=255)
                                                                   ttttCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGa                                                                                  <  1:679655/150‑1 (MQ=255)
                                                                     ttCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAg                                                                                <  2:1489434/150‑1 (MQ=255)
                                                                      tCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGaaag                                                                               <  2:1947870/150‑3 (MQ=255)
                                                                       cGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGc                                                                              >  1:2870707/1‑150 (MQ=255)
                                                                         tGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGTGACAGCACCAGCGTAGAGATGAAGGCGa                                                                            >  2:4297627/1‑150 (MQ=255)
                                                                         tGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGa                                                                            <  2:4532466/150‑1 (MQ=255)
                                                                          gCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGAt                                                                           <  2:2739636/150‑1 (MQ=255)
                                                                          gCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGAt                                                                           <  1:2974221/150‑1 (MQ=255)
                                                                          gCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCA‑‑‑‑‑‑‑‑‑ACAGCACCAGCGTAGAGATGAAGGCGATAATCAATaa                                                                  <  1:2093017/150‑1 (MQ=255)
                                                                           ccGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATa                                                                          <  2:2573498/150‑1 (MQ=255)
                                                                            cgcgTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATaa                                                                         >  2:222429/1‑150 (MQ=255)
                                                                                tGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCaa                                                                     >  2:3951046/1‑150 (MQ=255)
                                                                                  aaaTACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTGTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATa                                                                   >  1:2050218/1‑150 (MQ=255)
                                                                                   aaTACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATaa                                                                  >  2:3086815/1‑150 (MQ=255)
                                                                                       cGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTg                                                              >  1:3549706/1‑150 (MQ=255)
                                                                                       cGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTg                                                              <  2:528359/150‑1 (MQ=255)
                                                                                        gTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGc                                                             <  2:4396116/150‑1 (MQ=255)
                                                                                           gcgcAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGa                                                          <  1:2137564/150‑1 (MQ=255)
                                                                                            cgcAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGAt                                                         >  1:2036503/1‑150 (MQ=255)
                                                                                             gcAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATc                                                        <  1:164748/150‑1 (MQ=255)
                                                                                              cAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCt                                                       <  1:3274046/150‑1 (MQ=255)
                                                                                               aGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCtt                                                      <  1:891741/150‑1 (MQ=255)
                                                                                               aGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCtt                                                      <  1:1227129/150‑1 (MQ=255)
                                                                                                 cTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTAc                                                    <  2:3442809/150‑1 (MQ=255)
                                                                                                     cGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCgg                                                >  2:1949919/1‑150 (MQ=255)
                                                                                                     cGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCgg                                                <  2:2733197/150‑1 (MQ=255)
                                                                                                     cGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCgg                                                >  1:2591190/1‑150 (MQ=255)
                                                                                                           tGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATc                                          <  2:4470854/150‑1 (MQ=255)
                                                                                                            gCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATcc                                         <  1:512822/150‑1 (MQ=255)
                                                                                                              cAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCg                                       >  2:2971658/1‑150 (MQ=255)
                                                                                                               aGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGc                                      >  1:1744299/1‑150 (MQ=255)
                                                                                                                gCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGcc                                     >  2:3514020/1‑150 (MQ=255)
                                                                                                                 cACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGccc                                    >  2:2909180/1‑150 (MQ=255)
                                                                                                                    cGCAATCCAGCAGGCCATTGCCTGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCa                                 <  2:422229/150‑1 (MQ=255)
                                                                                                                    cGCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCa                                 >  2:1354555/1‑150 (MQ=255)
                                                                                                                     gCAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAg                                >  1:655502/1‑150 (MQ=255)
                                                                                                                      cAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCTATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAgc                               >  1:2844325/1‑150 (MQ=255)
                                                                                                                      cAATCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAgc                               >  2:885853/1‑150 (MQ=255)
                                                                                                                       aaTCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAgcg                              >  1:4508505/1‑150 (MQ=255)
                                                                                                                       aaTCCAGCAGGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAgcg                              >  1:4402612/1‑150 (MQ=255)
                                                                                                                           cagcagGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAGCGCAcc                          >  1:1222703/1‑150 (MQ=255)
                                                                                                                           cagcagGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAGCGCAcc                          <  1:3046201/150‑1 (MQ=255)
                                                                                                                            agcagGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAGCGCACCa                         <  1:156255/150‑1 (MQ=255)
                                                                                                                               agGCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAGCGCACCagag                      <  2:745600/150‑1 (MQ=255)
                                                                                                                                 gCCATTGCCAGGCCGAGCACAGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAGCGCACCAGAGGa                    <  1:2194116/150‑1 (MQ=255)
                                                                                                                                   cATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAGCGCACCAGAGGAcc                  <  1:2815827/150‑1 (MQ=255)
                                                                                                                                   cATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAGCGCACCAGAGGAcc                  <  1:4098140/150‑1 (MQ=255)
                                                                                                                                     ttGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAGCGCACCAGAGGACCGa                <  1:3906196/150‑1 (MQ=255)
                                                                                                                                      tGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAGCGCACCAGAGGACCGac               >  2:204157/1‑150 (MQ=255)
                                                                                                                                       gCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAGCGCACCAGAGGACCGaca              <  2:4236334/150‑1 (MQ=255)
                                                                                                                                        ccAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAGCGCACCAGAGGACCGacac             >  2:2878979/1‑150 (MQ=255)
                                                                                                                                         cAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAGCGCACCAGAGGACCGACAcc            >  1:2375578/1‑150 (MQ=255)
                                                                                                                                         cAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAGCGCACCAGAGGACCGACAcc            >  2:3993288/1‑150 (MQ=255)
                                                                                                                                           ggCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAGCGCACCAGAGGACCGACACCaa          >  2:4475840/1‑150 (MQ=255)
                                                                                                                                           ggCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAGCGCACCAGAGGACCGACACCaa          <  2:4264974/150‑1 (MQ=255)
                                                                                                                                               gAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAGCGCACCAGAGGACCGACACCAAGCAg      >  1:1794774/1‑150 (MQ=255)
                                                                                                                                                  cACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAGCGCACCAGAGGACCGACACCAAGCAGTAg   >  2:1283278/1‑150 (MQ=255)
                                                                                                                                                  cACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAGCGCACCAGAGGACCGACACCAAGCAGTAg   <  2:3758603/150‑1 (MQ=255)
                                                                                                                                                   aCCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAGCGCACCAGAGGACCGACACCAAGCAGTAgc  <  2:600706/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GCAATGCCAACAATTGTCACTGCCAGCCCAAGGTGAGCCGCCACCATCCCCCAATAACTGAAGGTGGTTTTCGTGCCGCGTGAAATACGTAGCGCAGCTTCCGCAATTGCCAGCACCGCAATCCAGCAGGCCATTGCCAGGCCGAGCACCGTCATCGCCACAACTTTGCTTTCGAACAGCCACGGCAACAGCAGCGACAGCACCAGCGTAGAGATGAAGGCGATAATCAATAAATTGCGGATCTTACGCGGGCGATCCCGCCCCCAGCGCACCAGAGGACCGACACCAAGCAGTAGC  >  CP009273/2286879‑2287175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑