Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,303,070 T→G 100% Q272P (CAG→CCG)  ada ← fused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,303,0700TG99.4% 1300.4 / ‑3.8 321Q272P (CAG→CCG) adafused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (141/178);  total (142/179)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 321 total aligned reads displayed.

AGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCTGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTG  >  CP009273/2302924‑2303216
                                                                                                                                                  |                                                                                                                                                  
    tattatGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGc                                                                                                                                             >  1:3758754/1‑150 (MQ=255)
    tattatGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGc                                                                                                                                             <  1:2131948/150‑1 (MQ=255)
    tattatGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGc                                                                                                                                             <  1:1138077/150‑1 (MQ=255)
     attatGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCa                                                                                                                                            <  1:3692577/150‑1 (MQ=255)
      ttatGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAg                                                                                                                                           >  1:1283684/1‑150 (MQ=255)
       tatGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGt                                                                                                                                          >  1:2520470/1‑150 (MQ=255)
        atGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTg                                                                                                                                         <  1:3678100/150‑1 (MQ=255)
        atGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTg                                                                                                                                         <  1:415794/150‑1 (MQ=255)
        atGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTg                                                                                                                                         >  1:2982257/1‑150 (MQ=255)
        atGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTg                                                                                                                                         <  2:576219/150‑1 (MQ=255)
        atGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTg                                                                                                                                         >  2:1848981/1‑150 (MQ=255)
        atGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGAGCCTGCCACACATGTTGCGGAAAAGCAGTg                                                                                                                                         >  2:3638189/1‑150 (MQ=255)
          gATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGcc                                                                                                                                       <  2:1191539/150‑1 (MQ=255)
           aTAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCcg                                                                                                                                      <  1:3030785/150‑1 (MQ=255)
           aTAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCcg                                                                                                                                      <  2:3707163/150‑1 (MQ=255)
           aTAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCcg                                                                                                                                      >  2:301261/1‑150 (MQ=255)
            tAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCcgc                                                                                                                                     >  1:221002/1‑150 (MQ=255)
             aGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCcgcg                                                                                                                                    <  1:3995330/150‑1 (MQ=255)
             aGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCcgcg                                                                                                                                    >  2:170952/1‑150 (MQ=255)
                cAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAAt                                                                                                                                 <  1:2045688/150‑1 (MQ=255)
                  gCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGt                                                                                                                               <  2:2270586/150‑1 (MQ=255)
                   cTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTc                                                                                                                              >  1:332097/1‑150 (MQ=255)
                   cTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTc                                                                                                                              <  1:3066267/150‑1 (MQ=255)
                     tgttgGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCa                                                                                                                            >  1:1808883/1‑150 (MQ=255)
                     tgttgGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCa                                                                                                                            <  1:3243983/150‑1 (MQ=255)
                      gttgGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAg                                                                                                                           <  1:969802/150‑1 (MQ=255)
                      gttgGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAg                                                                                                                           <  1:3351081/150‑1 (MQ=255)
                       ttgGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGc                                                                                                                          <  2:3283026/150‑1 (MQ=255)
                             gcACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAAc                                                                                                                    <  2:247066/150‑1 (MQ=255)
                                 aGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCa                                                                                                                <  1:146238/150‑1 (MQ=255)
                                      cTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAgcggc                                                                                                           <  1:1357361/150‑1 (MQ=255)
                                       tGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAgcggcg                                                                                                          >  1:3986256/1‑150 (MQ=255)
                                        ggCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGt                                                                                                         <  2:1129675/150‑1 (MQ=255)
                                         gCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTa                                                                                                        <  2:2696773/150‑1 (MQ=255)
                                          cAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTAt                                                                                                       <  2:2557114/150‑1 (MQ=255)
                                           aaCGTCCCGTACCGCTTTCGGTTTGCCGATTGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTAGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATc                                                                                                      >  2:750518/1‑150 (MQ=255)
                                           aaCGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATc                                                                                                      <  1:4366812/150‑1 (MQ=255)
                                            aCGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATcg                                                                                                     >  2:2756357/1‑150 (MQ=255)
                                              ggCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATcgcg                                                                                                   >  2:4174876/1‑150 (MQ=255)
                                               gCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGt                                                                                                  >  2:208910/1‑150 (MQ=255)
                                                cccGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGtt                                                                                                 <  1:2465873/150‑1 (MQ=255)
                                                 ccGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGttg                                                                                                <  1:1727023/150‑1 (MQ=255)
                                                   gTACCGCTTTCGGTTTGCCGATGTCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGttgat                                                                                              >  1:939159/1‑150 (MQ=255)
                                                      ccGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGttgattga                                                                                           <  1:100266/150‑1 (MQ=255)
                                                       cGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAg                                                                                          <  2:129438/150‑1 (MQ=255)
                                                       cGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAg                                                                                          <  1:4257237/150‑1 (MQ=255)
                                                       cGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAg                                                                                          <  1:1925551/150‑1 (MQ=255)
                                                       cGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAg                                                                                          >  1:635198/1‑150 (MQ=255)
                                                              ggTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCg                                                                                   >  2:4219145/1‑150 (MQ=255)
                                                              ggTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCg                                                                                   >  2:4055042/1‑150 (MQ=255)
                                                              ggTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCg                                                                                   <  1:1522741/150‑1 (MQ=255)
                                                               gTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGa                                                                                  <  1:1738989/150‑1 (MQ=255)
                                                                tttGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGAt                                                                                 >  1:3580799/1‑150 (MQ=255)
                                                                  tGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCa                                                                               >  1:4479175/1‑150 (MQ=255)
                                                                     cGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCTAATGACCAGCGGTAACGTCAGCGGCGTATCGCGTTGATGGAGGCTGGCGATCACtt                                                                            >  1:4360207/1‑150 (MQ=255)
                                                                     cGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACtt                                                                            <  2:2844205/150‑1 (MQ=255)
                                                                      gATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTc                                                                           >  2:3112628/1‑150 (MQ=255)
                                                                      gATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTc                                                                           <  2:613876/150‑1 (MQ=255)
                                                                        tGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCAc                                                                         <  2:3324431/150‑1 (MQ=255)
                                                                        tGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCAc                                                                         <  1:3010060/150‑1 (MQ=255)
                                                                               aGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATg                                                                  <  2:2635901/150‑1 (MQ=255)
                                                                                  cAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTg                                                               >  2:1352423/1‑150 (MQ=255)
                                                                                  cAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTg                                                               >  2:127417/1‑150 (MQ=255)
                                                                                  cAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTg                                                               <  2:2996193/150‑1 (MQ=255)
                                                                                    gTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCt                                                             <  2:3516514/150‑1 (MQ=255)
                                                                                        cTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGaaa                                                         >  1:439046/1‑150 (MQ=255)
                                                                                         tGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAAc                                                        >  2:3462267/1‑150 (MQ=255)
                                                                                              aCTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAg                                                   <  2:2521577/150‑1 (MQ=255)
                                                                                                tGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGTAACATCAGAt                                                 >  2:1403248/1‑150 (MQ=255)
                                                                                                  aCGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCg                                               <  2:2912290/150‑1 (MQ=255)
                                                                                                   cGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCgg                                              >  1:2174592/1‑150 (MQ=255)
                                                                                                   cGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGAATTGTTGCGGAAAAGCAGTGCCGCGAAAGTCCAGCGGTAACTTCAGCGGCGTATCGAGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATAgg                                              >  1:3962579/1‑150 (MQ=255)
                                                                                                      tttCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAg                                           <  1:3443857/150‑1 (MQ=255)
                                                                                                       ttCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAgg                                          >  2:713272/1‑150 (MQ=255)
                                                                                                           ccGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGcgcg                                      <  1:1062596/150‑1 (MQ=255)
                                                                                                           ccGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGcgcg                                      <  1:3814997/150‑1 (MQ=255)
                                                                                                            cGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGt                                     >  1:4266111/1‑150 (MQ=255)
                                                                                                             gCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGtt                                    <  2:4462904/150‑1 (MQ=255)
                                                                                                               aaGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGt                                  <  2:4366646/150‑1 (MQ=255)
                                                                                                                aGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTc                                 <  1:3549648/150‑1 (MQ=255)
                                                                                                                    aTCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCa                             >  1:1679808/1‑150 (MQ=255)
                                                                                                                    aTCGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCa                             <  1:2371322/150‑1 (MQ=255)
                                                                                                                      cGTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGc                           >  1:1682901/1‑150 (MQ=255)
                                                                                                                       gTGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCg                          <  1:557806/150‑1 (MQ=255)
                                                                                                                        tGCGCAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCgg                         <  2:3911643/150‑1 (MQ=255)
                                                                                                                          cgcAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGa                       >  1:2925740/1‑150 (MQ=255)
                                                                                                                            cAGTGCCTGCCAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGaaa                     >  1:1498543/1‑150 (MQ=255)
                                                                                                                               tgcctgccAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACAt                  <  2:3575406/150‑1 (MQ=255)
                                                                                                                                 cctgccAGTCTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACTCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATct                <  1:1434029/150‑1 (MQ=255)
                                                                                                                                 cctgccAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATct                >  2:3962860/1‑150 (MQ=255)
                                                                                                                                 cctgccAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATct                <  2:4313587/150‑1 (MQ=255)
                                                                                                                                 cctgccAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATct                >  2:159368/1‑150 (MQ=255)
                                                                                                                                 cctgccAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATct                <  2:1984030/150‑1 (MQ=255)
                                                                                                                                 cctgccAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATct                >  1:1612836/1‑150 (MQ=255)
                                                                                                                                  ctgccAGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATctg               <  2:2171578/150‑1 (MQ=255)
                                                                                                                                       aGACTTGTTGCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCa          <  2:234717/150‑1 (MQ=255)
                                                                                                                                            tgttgCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCg     <  1:2790014/150‑1 (MQ=255)
                                                                                                                                             gttgCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGc    >  1:4528233/1‑150 (MQ=255)
                                                                                                                                             gttgCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGc    >  1:468076/1‑150 (MQ=255)
                                                                                                                                              ttgCGGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCt   >  2:3232011/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGTTTGCCGATGGCGTTAGCCAGTTGCTGATAACTGACGGTTTCACCGCAAGGTATCGTGCGCAGTGCCTGCCAGACTTGTTGCTGAAAAGCAGTGCCGCGAATGTCCAGCGGTAACGTCAGCGGCGTATCGCGTTGATTGAGGCTGGCGATCACTTCACGCACATGTTGCTGAAACATCAGATCGGCAGGCGCGTTGTCGGCAGCGGGAAACATCTGCTGCAACTCGCTG  >  CP009273/2302924‑2303216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑