Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,352,573 C→A 100% P353P (CCG→CCT)  rhmT ← putative L‑rhamnonate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,352,5730CA97.4% 889.8 / 12.8 267P353P (CCG→CCT) rhmTputative L‑rhamnonate transporter
Reads supporting (aligned to +/- strand):  ref base C (4/3);  new base A (127/131);  total (131/136)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 267 total aligned reads displayed.

CCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCC  >  CP009273/2352428‑2352718
                                                                                                                                                 |                                                                                                                                                 
 cAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCAt                                                                                                                                              <  1:2735263/150‑1 (MQ=255)
  aGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATc                                                                                                                                             >  1:2902242/1‑150 (MQ=255)
     gTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTc                                                                                                                                          >  2:2690675/1‑150 (MQ=255)
       cAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCa                                                                                                                                        >  1:3061582/1‑150 (MQ=255)
          tAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGaa                                                                                                                                     >  2:2893509/1‑150 (MQ=255)
           aaTCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAac                                                                                                                                    >  1:216067/1‑150 (MQ=255)
            aTCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAaca                                                                                                                                   <  2:4637416/150‑1 (MQ=255)
            aTCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAaca                                                                                                                                   <  2:1466710/150‑1 (MQ=255)
                 gccgcATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGc                                                                                                                              <  2:3225978/150‑1 (MQ=255)
                  ccgcATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCt                                                                                                                             <  2:2376087/150‑1 (MQ=255)
                  ccgcATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCt                                                                                                                             <  2:79766/150‑1 (MQ=255)
                  ccgcATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCt                                                                                                                             <  2:2163036/150‑1 (MQ=255)
                  ccgcATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCt                                                                                                                             >  1:2849615/1‑150 (MQ=255)
                   cgcATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCGCCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTg                                                                                                                            <  1:1428225/150‑1 (MQ=255)
                      aTCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCAc                                                                                                                         >  1:441116/1‑150 (MQ=255)
                      aTCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCAc                                                                                                                         <  1:1003774/150‑1 (MQ=255)
                          cTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCa                                                                                                                     <  1:854552/150‑1 (MQ=255)
                           tGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCaa                                                                                                                    >  2:2829869/1‑150 (MQ=255)
                            ggCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAAt                                                                                                                   <  2:3806302/150‑1 (MQ=255)
                             gCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATa                                                                                                                  <  2:3497507/150‑1 (MQ=255)
                               aaaTAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATaaa                                                                                                                <  2:1830673/150‑1 (MQ=255)
                                aaTAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAAc                                                                                                               <  2:187113/150‑1 (MQ=255)
                                aaTAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAAc                                                                                                               >  1:2992167/1‑150 (MQ=255)
                                aaTAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAAc                                                                                                               >  1:4252011/1‑150 (MQ=255)
                                   aaCGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCg                                                                                                            <  2:4490026/150‑1 (MQ=255)
                                     cGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGAt                                                                                                          <  1:3602006/150‑1 (MQ=255)
                                      gTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATa                                                                                                         >  2:3650810/1‑150 (MQ=255)
                                       tttCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAg                                                                                                        >  1:3460194/1‑150 (MQ=255)
                                       tttCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAg                                                                                                        <  2:1221451/150‑1 (MQ=255)
                                        ttCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGc                                                                                                       <  2:1723180/150‑1 (MQ=255)
                                          cTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTg                                                                                                     >  2:3439621/1‑150 (MQ=255)
                                          cTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTg                                                                                                     >  2:2861235/1‑150 (MQ=255)
                                           tGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGc                                                                                                    <  2:2788346/150‑1 (MQ=255)
                                            gCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCa                                                                                                   <  1:4619988/150‑1 (MQ=255)
                                            gCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCa                                                                                                   >  2:917056/1‑150 (MQ=255)
                                                tCACGCGCACGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGcaccac                                                                                               >  1:3821701/1‑150 (MQ=255)
                                                tCACGCGCACGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAacac                                                                                               >  1:2777730/1‑150 (MQ=255)
                                                     cgcAGGATCGGGGCAATAAACGCGCCCTCTGCACCTATCAGGTTTACTTAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCTCTAGCTGAAACACACAgc                                                                                          <  2:3945459/150‑1 (MQ=255)
                                                     cgcAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAgc                                                                                          >  1:1341467/1‑150 (MQ=255)
                                                     cgcAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAgc                                                                                          <  2:3205234/150‑1 (MQ=255)
                                                     cgcAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAgc                                                                                          >  2:3121362/1‑150 (MQ=255)
                                                     cgcAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAgc                                                                                          <  2:2144270/150‑1 (MQ=255)
                                                     cgcAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAgc                                                                                          >  1:2316233/1‑150 (MQ=255)
                                                     cgcAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAgc                                                                                          <  1:4505764/150‑1 (MQ=255)
                                                     cgcAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAgc                                                                                          >  1:4068695/1‑150 (MQ=255)
                                                      gcAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAgcg                                                                                         >  1:486011/1‑150 (MQ=255)
                                                       cAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAgcgc                                                                                        <  1:3299477/150‑1 (MQ=255)
                                                         ggATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTa                                                                                      <  2:1258412/150‑1 (MQ=255)
                                                         ggATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTa                                                                                      <  2:2029009/150‑1 (MQ=255)
                                                           aTCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACg                                                                                    <  2:431066/150‑1 (MQ=255)
                                                             cGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGAt                                                                                  <  2:1019330/150‑1 (MQ=255)
                                                              ggggCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATc                                                                                 <  1:1427137/150‑1 (MQ=255)
                                                              ggggCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATc                                                                                 >  2:2746392/1‑150 (MQ=255)
                                                               gggCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCg                                                                                >  2:4000992/1‑150 (MQ=255)
                                                                    aTAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCAtt                                                                           >  1:1757562/1‑150 (MQ=255)
                                                                          ccGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGga                                                                     <  2:3452382/150‑1 (MQ=255)
                                                                           cGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGgag                                                                    >  1:389435/1‑150 (MQ=255)
                                                                           cGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGgag                                                                    <  1:2486587/150‑1 (MQ=255)
                                                                           cGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGgag                                                                    >  2:1890041/1‑150 (MQ=255)
                                                                            gCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGgaga                                                                   >  1:3253458/1‑150 (MQ=255)
                                                                             cccACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGaa                                                                  >  1:2059733/1‑150 (MQ=255)
                                                                               cACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAag                                                                >  2:2614734/1‑150 (MQ=255)
                                                                               cACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAag                                                                >  2:2915257/1‑150 (MQ=255)
                                                                               cACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAag                                                                <  2:390788/150‑1 (MQ=255)
                                                                               cACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAag                                                                <  2:966496/150‑1 (MQ=255)
                                                                                    cACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGcc                                                           >  1:469802/1‑150 (MQ=255)
                                                                                    cACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGcc                                                           <  1:1777738/150‑1 (MQ=255)
                                                                                    cACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGcc                                                           <  1:1135429/150‑1 (MQ=255)
                                                                                     aCCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGccc                                                          <  1:754027/150‑1 (MQ=255)
                                                                                     aCCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGccc                                                          <  1:1560236/150‑1 (MQ=255)
                                                                                     aCCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGccc                                                          >  1:1462571/1‑150 (MQ=255)
                                                                                        aaaCAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGa                                                       >  2:2457956/1‑150 (MQ=255)
                                                                                          aCAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACa                                                     >  2:1708171/1‑150 (MQ=255)
                                                                                             ggTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGac                                                  <  2:1133756/150‑1 (MQ=255)
                                                                                              gTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGacc                                                 >  1:2627279/1‑150 (MQ=255)
                                                                                              gTTTACAAAACCAAGTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGacc                                                 <  2:3061582/150‑1 (MQ=255)
                                                                                                 tACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaat                                              <  1:2391600/150‑1 (MQ=255)
                                                                                                    aaaaCCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccactacc                                           <  1:1234985/150‑1 (MQ=255)
                                                                                                    aaaaCCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaatacc                                           >  2:4133256/1‑150 (MQ=255)
                                                                                                       aCCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaataccaat                                        >  1:1005820/1‑150 (MQ=255)
                                                                                                       aCCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaataccaat                                        <  1:4471746/150‑1 (MQ=255)
                                                                                                          aaTTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGcc                                     >  2:868077/1‑150 (MQ=255)
                                                                                                           aTTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGccc                                    <  2:557846/150‑1 (MQ=255)
                                                                                                            ttCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCccg                                   >  2:1991575/1‑150 (MQ=255)
                                                                                                             tCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCccgc                                  >  1:3177843/1‑150 (MQ=255)
                                                                                                              cccGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCccgcc                                 >  2:3595480/1‑150 (MQ=255)
                                                                                                                cGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCcgccgc                               >  1:903890/1‑150 (MQ=255)
                                                                                                                 gCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCgccgcc                              >  1:3478923/1‑150 (MQ=255)
                                                                                                                      cagcGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTaa                         >  1:1833633/1‑150 (MQ=255)
                                                                                                                       agcGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAAt                        >  1:192669/1‑150 (MQ=255)
                                                                                                                       agcGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAAt                        >  2:1550951/1‑150 (MQ=255)
                                                                                                                         cgcCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGt                      >  1:2521421/1‑150 (MQ=255)
                                                                                                                            cGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAg                   <  2:4439350/150‑1 (MQ=255)
                                                                                                                              tACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAgcg                 >  1:2963576/1‑150 (MQ=255)
                                                                                                                              tACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAgcg                 >  1:643948/1‑150 (MQ=255)
                                                                                                                                ccGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCt               <  1:4173626/150‑1 (MQ=255)
                                                                                                                                 cGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTg              >  2:4533504/1‑150 (MQ=255)
                                                                                                                                 cGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTg              >  2:69604/1‑150 (MQ=255)
                                                                                                                                             gtgtCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGcc  <  2:1079318/150‑1 (MQ=255)
                                                                                                                                             gtgtAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGcc  <  2:4068695/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCC  >  CP009273/2352428‑2352718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑