Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,403,485 C→A 35.4% L449L (CTG→CTT)  yfbS ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,403,4850CA35.4% 442.8 / 235.6 260L449L (CTG→CTT) yfbSputative transporter
Reads supporting (aligned to +/- strand):  ref base C (89/78);  new base A (46/47);  total (135/125)
Fisher's exact test for biased strand distribution p-value = 6.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 266 total aligned reads displayed.

GTCCATCAGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTAAGTTCAGCGCTAC  >  CP009273/2403338‑2403634
                                                                                                                                                   |                                                                                                                                                     
  ccATCAGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATcagca                                                                                                                                                   <  1:2913794/150‑1 (MQ=255)
   cATCAGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATcagcag                                                                                                                                                  <  2:446498/150‑1 (MQ=255)
   cATCAGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATcagcag                                                                                                                                                  <  2:1858732/150‑1 (MQ=255)
    aTCAGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAgcagg                                                                                                                                                 <  2:4086225/150‑1 (MQ=255)
       aGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGTTCGGCCAGTGACTGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAgcaggcag                                                                                                                                              >  1:2393929/1‑150 (MQ=255)
         cccTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCaggcaggc                                                                                                                                            >  2:3518415/1‑150 (MQ=255)
          ccTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCg                                                                                                                                           <  1:3779852/150‑1 (MQ=255)
            tttCACCGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCgat                                                                                                                                         <  2:1227973/150‑1 (MQ=255)
            tttCACCGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCgat                                                                                                                                         <  2:1881034/150‑1 (MQ=255)
             ttCACCGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCgatg                                                                                                                                        >  1:1543240/1‑150 (MQ=255)
              tCACCGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCgatga                                                                                                                                       >  1:4153644/1‑150 (MQ=255)
               cACCGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCgatgat                                                                                                                                      >  1:2205864/1‑150 (MQ=255)
                 ccGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAg                                                                                                                                    <  1:216816/150‑1 (MQ=255)
                   gCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCg                                                                                                                                  <  2:771381/150‑1 (MQ=255)
                     cAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATACTGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGc                                                                                                                                <  2:952233/150‑1 (MQ=255)
                     cAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGc                                                                                                                                <  2:4414736/150‑1 (MQ=255)
                        cgcgACCCCTCCCGTTTACTGTACTGCCACAGCAAACGGCATCATCCCAACGATCAAAAGAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCCGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAAc                                                                                                                             <  2:3573922/150‑1 (MQ=255)
                        cgcgACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAAc                                                                                                                             >  1:4452648/1‑150 (MQ=255)
                        cgcgACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAAc                                                                                                                             >  1:4453350/1‑150 (MQ=255)
                           gACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAgg                                                                                                                          <  2:609423/150‑1 (MQ=255)
                            acacCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGa                                                                                                                         <  2:283335/150‑1 (MQ=255)
                             cacCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGAt                                                                                                                        <  1:264608/150‑1 (MQ=255)
                               ccTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTCTACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTa                                                                                                                      >  2:1173941/1‑150 (MQ=255)
                                cTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAg                                                                                                                     <  1:1351783/150‑1 (MQ=255)
                                 tCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAgg                                                                                                                    <  1:470215/150‑1 (MQ=255)
                                      ttttCTGTAATGCCACAGCAAACGGCATCCTCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAAttt                                                                                                               <  2:2172269/150‑1 (MQ=255)
                                      ttttCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAAttt                                                                                                               >  2:2656213/1‑150 (MQ=255)
                                      ttttCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAAttt                                                                                                               <  1:4248036/150‑1 (MQ=255)
                                       tttCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTc                                                                                                              <  1:224229/150‑1 (MQ=255)
                                            gTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTg                                                                                                         >  1:2964494/1‑150 (MQ=255)
                                             tAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGt                                                                                                        <  2:3995042/150‑1 (MQ=255)
                                               aTGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCa                                                                                                      <  2:1337403/150‑1 (MQ=255)
                                                tGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAg                                                                                                     <  1:3019990/150‑1 (MQ=255)
                                                 gCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGc                                                                                                    >  2:2990198/1‑150 (MQ=255)
                                                  ccACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCa                                                                                                   >  1:1884702/1‑150 (MQ=255)
                                                   cacaGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCAt                                                                                                  >  2:1751327/1‑150 (MQ=255)
                                                   cacaGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCAt                                                                                                  >  2:3433517/1‑150 (MQ=255)
                                                   cacaGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCAt                                                                                                  >  2:274262/1‑150 (MQ=255)
                                                   cacaGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCAt                                                                                                  >  2:301896/1‑150 (MQ=255)
                                                    acaGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCAtt                                                                                                 <  1:3322853/150‑1 (MQ=255)
                                                          aaCGGCATCATCCCCACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGcc                                                                                           <  1:3491914/150‑1 (MQ=255)
                                                           aCGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGcca                                                                                          >  1:2023137/1‑150 (MQ=255)
                                                             ggCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCCTTAACGccacc                                                                                        >  2:456555/1‑150 (MQ=255)
                                                             ggCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGccacc                                                                                        >  2:1799356/1‑150 (MQ=255)
                                                               catcatCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCAt                                                                                      <  2:1951546/150‑1 (MQ=255)
                                                                atcatcCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCAtt                                                                                     <  1:4555799/150‑1 (MQ=255)
                                                                atcatcCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCAtt                                                                                     <  1:1608145/150‑1 (MQ=255)
                                                                   atcCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGc                                                                                  <  2:1375859/150‑1 (MQ=255)
                                                                       cAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACca                                                                              >  1:858507/1‑150 (MQ=255)
                                                                           gATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACcagaca                                                                          >  1:707685/1‑150 (MQ=255)
                                                                              cAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGaa                                                                       >  1:82210/1‑150 (MQ=255)
                                                                              cAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGaa                                                                       >  2:2011242/1‑150 (MQ=255)
                                                                               aaaaTAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGaaa                                                                      >  2:3899886/1‑150 (MQ=255)
                                                                               aaaaTAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGaaa                                                                      >  2:4117863/1‑150 (MQ=255)
                                                                                aaaTAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGaaaa                                                                     >  2:3571327/1‑150 (MQ=255)
                                                                                aaaTAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGaaaa                                                                     >  1:3832863/1‑150 (MQ=255)
                                                                                 aataatGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAAt                                                                    >  2:3175391/1‑150 (MQ=255)
                                                                                 aataatGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAAt                                                                    <  2:1200581/150‑1 (MQ=255)
                                                                                      tGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCAt                                                               >  1:252087/1‑150 (MQ=255)
                                                                                       gcacGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATg                                                              <  2:4378997/147‑1 (MQ=255)
                                                                                        cTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATgg                                                             >  1:1126902/1‑150 (MQ=255)
                                                                                        cTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATgg                                                             <  1:4337118/150‑1 (MQ=255)
                                                                                         tCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATggg                                                            >  1:1364661/1‑150 (MQ=255)
                                                                                          cGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATgggg                                                           <  2:3947794/150‑1 (MQ=255)
                                                                                          cGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATgggg                                                           <  1:3030407/150‑1 (MQ=255)
                                                                                           ggCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGt                                                          >  1:1723776/1‑150 (MQ=255)
                                                                                             ccAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGc                                                        >  1:2871914/1‑150 (MQ=255)
                                                                                               aGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCt                                                      <  2:2596619/150‑1 (MQ=255)
                                                                                               aGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCt                                                      >  1:4566599/1‑150 (MQ=255)
                                                                                                  gAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGc                                                   >  2:1647031/1‑150 (MQ=255)
                                                                                                     tGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCtgt                                                <  1:801883/150‑1 (MQ=255)
                                                                                                     tGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCtgt                                                >  2:3347254/1‑150 (MQ=255)
                                                                                                       gATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTgc                                              >  2:2334409/1‑150 (MQ=255)
                                                                                                       gATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTgc                                              <  2:190421/150‑1 (MQ=255)
                                                                                                        aTTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTgcg                                             >  1:2019058/1‑150 (MQ=255)
                                                                                                          ttATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGcgcg                                           <  1:1028438/150‑1 (MQ=255)
                                                                                                            ataGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCggg                                         >  1:123426/1‑150 (MQ=255)
                                                                                                             taGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGt                                        >  2:3245262/1‑150 (MQ=255)
                                                                                                              aGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTg                                       <  1:4452585/150‑1 (MQ=255)
                                                                                                                 cgcTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATg                                    <  2:3284672/150‑1 (MQ=255)
                                                                                                                  gcTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAGCAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGc                                   >  2:426267/1‑150 (MQ=255)
                                                                                                                      tCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTtca                               >  2:4391694/1‑150 (MQ=255)
                                                                                                                      tCAGCATCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTtca                               >  1:1517915/1‑150 (MQ=255)
                                                                                                                        aGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTtcact                             >  1:2784234/1‑150 (MQ=255)
                                                                                                                           aTCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAAAAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTtcactcac                          >  1:873581/1‑150 (MQ=255)
                                                                                                                            tCTATACAGCGGAATTTCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCAcc                         <  2:715035/150‑1 (MQ=255)
                                                                                                                             cTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACct                        <  2:2233911/150‑1 (MQ=255)
                                                                                                                               ataCAGCTGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCATCAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACctct                      <  2:1006302/150‑1 (MQ=255)
                                                                                                                               ataCAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACctct                      <  2:3330219/150‑1 (MQ=255)
                                                                                                                                 aCAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTc                    >  1:3302087/1‑150 (MQ=255)
                                                                                                                                      ggAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTa               >  2:644795/1‑150 (MQ=255)
                                                                                                                                       gAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTaa              >  1:2676847/1‑150 (MQ=255)
                                                                                                                                          tttCCCCATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTAAGtt           >  2:2500904/1‑150 (MQ=255)
                                                                                                                                           ttCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTAAGTTc          >  1:3597193/1‑150 (MQ=255)
                                                                                                                                            tCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTAAGTTCa         >  2:4207673/1‑150 (MQ=255)
                                                                                                                                             ccccATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTAAGTTCAg        <  2:2953511/150‑1 (MQ=255)
                                                                                                                                               ccATAAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTAAGTTCAgcg      <  2:2776074/150‑1 (MQ=255)
                                                                                                                                                 aTCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTAAGTTCAGCGCt    <  2:4421303/150‑1 (MQ=255)
                                                                                                                                                 aTCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTAAGTTCAGCGCt    <  2:3807793/150‑1 (MQ=255)
                                                                                                                                                   cagcagGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTAAGTTCAGCGCTAc  >  1:3141415/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GTCCATCAGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGTAATGCCACAGCAAACGGCATCATCCCAACGATCAAAATAATGCTCGGCCAGTGAATGGATTTATAGGCGCTTTCAGCATCTATACAGCGGAATTTCCCCATCAGCAGGCAGGCGATGATAGCGGCAACAGGATTAGGAATTTCATCTGTCAGCATTAACGCCACCATTAGCACCAGACAGAAAATGGCATGGGGTGCCTGGCTGTGCGCGGGTGATGCTTCACTCACCTCTTCCGGTAAGTTCAGCGCTAC  >  CP009273/2403338‑2403634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑