Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,404,742 A→C 13.3% A30A (GCT→GCG)  yfbS ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,404,7420AC13.3% 504.6 / 30.8 238A30A (GCT→GCG) yfbSputative transporter
Reads supporting (aligned to +/- strand):  ref base A (115/87);  new base C (2/33);  total (118/120)
Fisher's exact test for biased strand distribution p-value = 4.02e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 252 total aligned reads displayed.

CCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGA  >  CP009273/2404593‑2404890
                                                                                                                                                     |                                                                                                                                                    
cccATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAAcaaacaaa                                                                                                                                                      >  1:1551257/1‑150 (MQ=255)
cccATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAAcaaacaaa                                                                                                                                                      >  2:3642367/1‑150 (MQ=255)
 ccATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAg                                                                                                                                                     <  1:3379647/150‑1 (MQ=255)
 ccATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAg                                                                                                                                                     <  1:2156358/150‑1 (MQ=255)
    ttACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGa                                                                                                                                                  <  2:117990/150‑1 (MQ=255)
     tACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGAc                                                                                                                                                 >  1:2637650/1‑150 (MQ=255)
       gggTGGCAACACCGGTAGGGACCAAACAATCGCCAATAATAAACAAGGCGGCAATCTGGACAAGGTTAGTATCTGAAAAGCCGGAAATTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCg                                                                                                                                               <  2:4334021/149‑1 (MQ=255)
       cGGTGGCAACACCGGTACGGACCAAACCATCGCAAATAATAAACATGGCGGCAATCAGGACAACGTTTGGATCAGAAAAGCCGTAAAATACTTCTGGGACTGTCAGCGTTCCGCTTATTGCAAACGCGACAATAACAACCAAAGCGACCg                                                                                                                                               <  1:1476601/150‑1 (MQ=255)
                  ccGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGc                                                                                                                                    >  2:3978923/1‑150 (MQ=255)
                   cGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCa                                                                                                                                   >  2:2811594/1‑150 (MQ=255)
                     ttACGGACCAAACAAGCGAAACTAATAAACATGGGGGCAATCATGACAACGTTAGGATATGAAATGCCTTAAATTACTTCTGGTACTGTCAGCGTTCCGCTAACTGCAAACGCGACAATACCAAACAAAGCGACCGCATCCATACGCAct                                                                                                                                 <  2:2297016/149‑1 (MQ=255)
                       aCGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCActct                                                                                                                               >  2:2071950/1‑150 (MQ=255)
                             cAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGt                                                                                                                         >  1:2991580/1‑150 (MQ=255)
                              aaaCCATCGAAAATAATAAACAAGGCGGCAATCAGGAAAACGTTAGTATAATAAAAGCCTGAAAATAATTCTGGGACTGTCTGCTTTCCGATTAATTAAAACGCGACAATAAAAAAAAAAGCGACCGCATCCATACGCACTCTGCCCGTc                                                                                                                        <  1:3017922/150‑1 (MQ=255)
                                acaaTCGCCAATAATAAACATGGCGTAAATAAGCACAACGTTCGGATCATAAACGCGGGAAAATAGTTGTGGTAGTGTCAGCGTTCCGTTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGc                                                                                                                      <  2:107772/147‑1 (MQ=255)
                                acaaTCGCAAATAAAAAACACGGCGGAATTCTGGAAAATGTTTTTTTCTGAAATGCCGTAAATTACTTTGGGTATTGTATGGGTTCATATTTATGAAAACGCTAAAATAAAAAACTAAGCGACCGCATCCATACGCACTCTGCCCGTCGc                                                                                                                      <  2:107761/147‑1 (MQ=255)
                                  cATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCaa                                                                                                                    >  1:3933891/1‑150 (MQ=255)
                                   atcccaaataaaAAACACGGCGCAAATCAGGACAACGTTAGCATAAGAAAAGCCGTAAAATACTTCTGGGACTGTCAGCGTTCCGATTAATGCAAACGCGAAAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCaaa                                                                                                                   <  1:2244802/144‑1 (MQ=255)
                                    ccGCAAATAATAAACACGGCGTAATCCTGGACAAGTTTTGTTTAAGAAACGCCGTAAAATACTTGGGGTACTGTCTGCGTTCCGTTTAATGCAAACGCTACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAAc                                                                                                                  <  2:1615656/149‑1 (MQ=255)
                                     cGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACa                                                                                                                 >  1:4357871/1‑150 (MQ=255)
                                     cGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACa                                                                                                                 >  1:1406582/1‑150 (MQ=255)
                                         aataatAAACATGGGGTCAATCTGTACAATTTTATTATAATAAACGCCGTAAATTACTTGGGGGACTGTCTGGTTTCCGCTTATTGAAAACGCTAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGAc                                                                                                             <  2:1193088/150‑1 (MQ=255)
                                         aataatAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGAc                                                                                                             >  2:1395908/1‑150 (MQ=255)
                                           taataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGa                                                                                                           >  1:551703/1‑150 (MQ=255)
                                              taaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATgg                                                                                                        >  2:2596128/1‑150 (MQ=255)
                                              taaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATgg                                                                                                        >  2:2596740/1‑150 (MQ=255)
                                               aaaCACGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCTGCGTTCCGCTTAATGCAAACGCGACAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGc                                                                                                       <  2:331272/150‑1 (MQ=255)
                                               aaaCAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGc                                                                                                       >  1:194996/1‑150 (MQ=255)
                                                aaCACGGCGGCAATCCGCAAAACTTTTGTATACTAAAAGCGGTAAAATCCTCCTGGGACTGTCCGGGTTCCGATTAATGCAAACGCCAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCa                                                                                                      <  1:4484504/150‑1 (MQ=255)
                                                 acacGGCGCCAATCAGCAAACCTTTAGTATCATAAAAGCCGTAAAATATTTGTGGGAGTGTCTGGTTTCGGTTTAATGCAAACCCCACAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCaa                                                                                                     <  1:245255/150‑1 (MQ=255)
                                                  cacggcggaAATCCGTACAACGTTTGTAACATAAATGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAAc                                                                                                    <  1:1877228/147‑1 (MQ=255)
                                                   aaGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACg                                                                                                   >  2:1387326/1‑150 (MQ=255)
                                                      gcggcAATCAGGACAACGTTAGTATCATAAAAGCCGTAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGAAAACGCGAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGcc                                                                                                <  2:326271/150‑1 (MQ=255)
                                                        ggcAATCAGTACAACGTTAGTATCATAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAACCGCGACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAg                                                                                              <  1:3460877/150‑1 (MQ=255)
                                                         gcAATCTGCAAACCGTTAGTATCAGAAAAGCCGTAAAATACTTCTGGGACGGTCTGCTTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGt                                                                                             <  1:3741214/150‑1 (MQ=255)
                                                          cAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTa                                                                                            >  1:3650484/1‑150 (MQ=255)
                                                           aaTCAGTAAACCGTTTGTATCACAAAAGCCGTAAAATACTTCTGGTACTTTCAGCGTTCCGCTTAATGCAACCGCTAAAAAAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTaa                                                                                           <  2:835568/150‑1 (MQ=255)
                                                             tCAGTACATCGTTTGTATAATAAATGCCGTAAAATAGTTCTGGTACTGTCCTGGTTCGGCTTAATGCTAACGCTAAAATAACAACCAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATg                                                                                         <  2:339501/150‑1 (MQ=255)
                                                             tCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATg                                                                                         <  2:350664/150‑1 (MQ=255)
                                                               aGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGaa                                                                                       >  2:357461/1‑150 (MQ=255)
                                                                ggACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGaaa                                                                                      >  2:856428/1‑150 (MQ=255)
                                                                    aaCGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAAc                                                                                  >  2:2327259/1‑150 (MQ=255)
                                                                      cGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAAccc                                                                                >  1:1125772/1‑150 (MQ=255)
                                                                         tAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCaaa                                                                             <  2:753479/150‑1 (MQ=255)
                                                                          aGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAt                                                                            >  2:1483996/1‑150 (MQ=255)
                                                                             aTCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCaa                                                                         >  1:2198788/1‑150 (MQ=255)
                                                                              tCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAt                                                                        >  2:3121015/1‑150 (MQ=255)
                                                                               aaGAAAAGCCGGAAAATAATTCTGGGATTTTCAGCTTTCCGCTTAATGCAAACGCTAAAATAAAAAAAAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAtt                                                                       <  2:1217498/149‑1 (MQ=255)
                                                                                ataaacGCCGTAAAATACTTCGGGGACTGTCTGCTTCCCGCTTAATGCAACCGCTAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTc                                                                      <  1:2375013/148‑1 (MQ=255)
                                                                                aGAAAAGCGGTAAAATAGTTGTGGGACTGTCCGGGTTCCGATTAATGAAACCGCTAAAATAAAAAAAAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTc                                                                      <  1:3733839/150‑1 (MQ=255)
                                                                                  aaaaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAc                                                                    >  2:3850770/1‑150 (MQ=255)
                                                                                   aatgcgggAAAATACTTGTGGTACTGTCTGGGTTCCGATTAATGAAAACGCTAAAATAACAAAAAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAcc                                                                   <  1:1094853/144‑1 (MQ=255)
                                                                                   aaaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAcc                                                                   >  2:512015/1‑150 (MQ=255)
                                                                                    aaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCg                                                                  <  2:290925/150‑1 (MQ=255)
                                                                                    aaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCg                                                                  >  1:207547/1‑150 (MQ=255)
                                                                                     tgacGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTATTTAAAACGCGACAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGt                                                                 <  2:2263800/147‑1 (MQ=255)
                                                                                      gCCGTAAATTACTTCTGGGACTGTCAGCTTTCCTATTAATGAAAACGCTAAAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGtt                                                                <  1:2742406/150‑1 (MQ=255)
                                                                                       ccGTAAATTACTTCTGGGAGTGTCCGGGTTCCGATTAATGCAAACGCGACAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTc                                                               <  1:3388384/150‑1 (MQ=255)
                                                                                        tgtaaatTAATTTTGGTACTGTCTGGGTTCCGATTAATTAAAACGCTAAAAAAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTca                                                              <  1:3492952/147‑1 (MQ=255)
                                                                                         ggAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTcac                                                             >  1:4310513/1‑150 (MQ=255)
                                                                                         ggAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTcac                                                             >  1:605596/1‑150 (MQ=255)
                                                                                          taaaaTACTTGTGGTACTGTCTGCGTTCCGATTATTGAAAACGCTACAATAACAAAAAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTcaca                                                            <  1:1842999/149‑1 (MQ=255)
                                                                                          gAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTcaca                                                            >  1:4198302/1‑150 (MQ=255)
                                                                                             aaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACt                                                         >  2:2234917/1‑150 (MQ=255)
                                                                                             aaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACt                                                         >  2:2234960/1‑150 (MQ=255)
                                                                                              aTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACtt                                                        <  2:1698812/150‑1 (MQ=255)
                                                                                                aCTTTTGGGACTGTCCGGGTACCTCTTAATCAAAACGCTAAAAAAAAAACCCAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTAt                                                      <  1:4399495/150‑1 (MQ=255)
                                                                                                aCTTCTGGGACTGTCCGCGTTCCGCTTAATCCAAACGCCAAAAAAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTAt                                                      <  1:1661334/150‑1 (MQ=255)
                                                                                                aCTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTAt                                                      >  2:686082/1‑150 (MQ=255)
                                                                                                aCTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTAt                                                      <  1:3063154/150‑1 (MQ=255)
                                                                                                   tCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCt                                                   <  2:3062737/150‑1 (MQ=255)
                                                                                                     tGGTACTGTCTGCTTTCCGCTTAATTCAACCTCCAAAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTg                                                 <  1:4457256/150‑1 (MQ=255)
                                                                                                       tgACTGTCAGGGTTCCTCTTAATGCAACGCCTACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGAAGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGtt                                               <  1:500689/149‑1 (MQ=255)
                                                                                                       ggACTGTCAGCGTTCCGCTTAATGCAACCGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGtt                                               <  2:2715176/150‑1 (MQ=255)
                                                                                                       ggACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGtt                                               <  2:4230108/150‑1 (MQ=255)
                                                                                                       ggACTGTCAGCGTTCCGATTAATGAAACCGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGtt                                               <  2:2801792/150‑1 (MQ=255)
                                                                                                            gTCAGCGTCCCGATTAATCCAACCGCTACAAAAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTg                                          <  1:1975091/150‑1 (MQ=255)
                                                                                                             tCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGa                                         >  2:97813/1‑150 (MQ=255)
                                                                                                             tCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGa                                         >  1:3591189/1‑150 (MQ=255)
                                                                                                               aGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAgg                                       >  2:2436368/1‑150 (MQ=255)
                                                                                                                 cGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAgggg                                     >  2:3847668/1‑150 (MQ=255)
                                                                                                                    tCCGCTTAATGCAAACGCGACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGAt                                  <  1:1891548/150‑1 (MQ=255)
                                                                                                                    tCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGAt                                  >  2:2851051/1‑150 (MQ=255)
                                                                                                                      cGCTTAATGCAAACGCTAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGa                                <  2:1697286/150‑1 (MQ=255)
                                                                                                                      cGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGa                                <  2:3774061/150‑1 (MQ=255)
                                                                                                                       gCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGAc                               >  1:712276/1‑150 (MQ=255)
                                                                                                                        gttAATGCAAACGCGACAATAACAAACAAAGCGACCGCAACCATACGCACTCTGCACGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACt                              >  1:38735/2‑150 (MQ=255)
                                                                                                                               cAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCATTGTTAATTGAGGGGGATGACTTGATTCt                       >  1:2601023/1‑150 (MQ=255)
                                                                                                                                  ccgcgACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGcc                    <  2:2136467/149‑1 (MQ=255)
                                                                                                                                   cgcgACAATAACAAACAATGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTGGTTAATTGAGGGGGATGACTTGATTCTGCCa                   >  2:2097949/1‑150 (MQ=255)
                                                                                                                                      gACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaa                <  2:4557352/150‑1 (MQ=255)
                                                                                                                                      gACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaa                >  2:4102539/1‑150 (MQ=255)
                                                                                                                                          aTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaaaaa            <  2:4379456/150‑1 (MQ=255)
                                                                                                                                           tAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAg           <  2:409450/150‑1 (MQ=255)
                                                                                                                                            aacaaacaAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGc          >  1:3676540/1‑150 (MQ=255)
                                                                                                                                             acaaacaaAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGcc         >  1:1156722/1‑150 (MQ=255)
                                                                                                                                             acaaacaaAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGcc         <  1:1026917/150‑1 (MQ=255)
                                                                                                                                               aaccaaaGCGACCTCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGcccc       <  1:4072521/147‑1 (MQ=255)
                                                                                                                                                aacaaaGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcg      <  2:2991580/150‑1 (MQ=255)
                                                                                                                                                   aaaGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcgacg   <  2:1827571/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGA  >  CP009273/2404593‑2404890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑