Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,405,946 T→G 94.2% Q43P (CAG→CCG)  yfbU ← UPF0304 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,405,9460TG94.2% 967.7 / 22.1 251Q43P (CAG→CCG) yfbUUPF0304 family protein
Reads supporting (aligned to +/- strand):  ref base T (3/12);  new base G (124/112);  total (127/124)
Fisher's exact test for biased strand distribution p-value = 1.66e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

AAGGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACT  >  CP009273/2405799‑2406092
                                                                                                                                                   |                                                                                                                                                  
aaGGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGt                                                                                                                                                  >  2:3964587/1‑150 (MQ=255)
aaGGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGt                                                                                                                                                  >  2:2145415/1‑150 (MQ=255)
 aGGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTa                                                                                                                                                 <  2:1128149/150‑1 (MQ=255)
  ggTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATGGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTaa                                                                                                                                                <  2:2609027/150‑1 (MQ=255)
        acgacgTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTa                                                                                                                                          >  1:1458285/1‑150 (MQ=255)
           acgTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAAcc                                                                                                                                       >  1:909240/1‑150 (MQ=255)
             gTTCATCGATGGATTGCTGGTCCTGCAAATTAGTCCAGGAAACATGCAACGCATGATACATCTCCAGAATGTCGATGATAGTGCGGCTGGTTTCTTATTTCAGCTCGCCAAACTCGCGATGCAATTCACGCTTCGGTAATCCGTAACCAc                                                                                                                                     <  2:1860226/150‑1 (MQ=255)
             gTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCAc                                                                                                                                     >  2:3484884/1‑150 (MQ=255)
             gTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCAc                                                                                                                                     <  1:2867375/150‑1 (MQ=255)
             gTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCAc                                                                                                                                     >  1:3835173/1‑150 (MQ=255)
              ttCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACg                                                                                                                                    <  1:1297781/150‑1 (MQ=255)
               tCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTGTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGc                                                                                                                                   >  2:464334/1‑150 (MQ=255)
                cATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCt                                                                                                                                  <  1:19118/150‑1 (MQ=255)
                 aTCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTc                                                                                                                                 <  2:4543381/150‑1 (MQ=255)
                 aTCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTc                                                                                                                                 <  1:2334758/150‑1 (MQ=255)
                  tCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCa                                                                                                                                <  1:2429368/150‑1 (MQ=255)
                     aTGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCaata                                                                                                                             >  2:4029945/1‑150 (MQ=255)
                       ggATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCaataat                                                                                                                           <  1:2910264/150‑1 (MQ=255)
                       ggATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCaataat                                                                                                                           >  1:2505335/1‑150 (MQ=255)
                        gATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAAtt                                                                                                                          <  1:4470816/150‑1 (MQ=255)
                        gATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAAtt                                                                                                                          <  2:2667346/150‑1 (MQ=255)
                        gATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAAtt                                                                                                                          <  1:2396567/150‑1 (MQ=255)
                         aTTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTg                                                                                                                         >  2:4195271/1‑150 (MQ=255)
                                  ccTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGc                                                                                                                >  2:3159087/1‑150 (MQ=255)
                                     gCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGAc                                                                                                             >  1:2078652/1‑150 (MQ=255)
                                      cAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACg                                                                                                            <  2:3730528/150‑1 (MQ=255)
                                      cAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACg                                                                                                            <  1:3370016/150‑1 (MQ=255)
                                      cAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACg                                                                                                            >  1:2462690/1‑150 (MQ=255)
                                       aaaTTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACgg                                                                                                           >  2:1073490/1‑150 (MQ=255)
                                       aaaTTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTATGCAGGCGACgg                                                                                                           >  2:1501567/1‑150 (MQ=255)
                                          ttAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTaa                                                                                                        >  2:2262859/1‑150 (MQ=255)
                                           tAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAAc                                                                                                       <  1:26652/150‑1 (MQ=255)
                                              aCCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGtt                                                                                                    >  2:4242324/1‑150 (MQ=255)
                                              aCCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGtt                                                                                                    >  1:3907953/1‑150 (MQ=255)
                                              aCCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGtt                                                                                                    >  2:2347263/1‑150 (MQ=255)
                                               ccAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTc                                                                                                   >  2:3392148/1‑150 (MQ=255)
                                                cAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCa                                                                                                  <  1:328734/150‑1 (MQ=255)
                                                  ggAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGc                                                                                                >  1:3453335/1‑150 (MQ=255)
                                                   gAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCa                                                                                               <  2:2715421/150‑1 (MQ=255)
                                                   gAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCa                                                                                               >  2:2075930/1‑150 (MQ=255)
                                                      aCATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCAttt                                                                                            <  2:3423569/150‑1 (MQ=255)
                                                      aCATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCAttt                                                                                            <  1:4591903/150‑1 (MQ=255)
                                                       cATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTg                                                                                           <  1:1163373/150‑1 (MQ=255)
                                                       cATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTg                                                                                           <  1:2255773/150‑1 (MQ=255)
                                                         tGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGcc                                                                                         <  1:1327804/150‑1 (MQ=255)
                                                          gCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCg                                                                                        <  2:1444642/150‑1 (MQ=255)
                                                           catagCATGATACTTCTCCCTAATGTCGTTGATTGTGAGGCAGGTTTCTTCTTTCAGCTCGCAAACCTCGAGATCCAATTCAAGCATCGGTAATCCGTATCCACGCTCTTTTATTGTTTTCAGGGGACGGTTACGTTCTTCTTTTGCCgg                                                                                       <  2:2775954/146‑1 (MQ=255)
                                                            aaCGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGa                                                                                      <  1:3076495/150‑1 (MQ=255)
                                                              cGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATc                                                                                    >  1:4108547/1‑150 (MQ=255)
                                                                 aTGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAg                                                                                 >  2:2008929/1‑150 (MQ=255)
                                                                    aTACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCAt                                                                              >  2:3354717/1‑150 (MQ=255)
                                                                     tACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATa                                                                             <  1:1588063/150‑1 (MQ=255)
                                                                          ctcCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGtcat                                                                        <  1:733455/150‑1 (MQ=255)
                                                                            ccATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGtcatca                                                                      >  2:4113959/1‑150 (MQ=255)
                                                                            ccATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGtcatca                                                                      >  2:919217/1‑150 (MQ=255)
                                                                               tAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCt                                                                   <  1:1827589/150‑1 (MQ=255)
                                                                               tAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCt                                                                   <  2:261336/150‑1 (MQ=255)
                                                                                     cGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACt                                                             <  2:3100181/150‑1 (MQ=255)
                                                                                     cGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACt                                                             <  2:777900/150‑1 (MQ=255)
                                                                                      gatgatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTg                                                            <  2:1239567/150‑1 (MQ=255)
                                                                                      gatgatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTg                                                            >  2:3948351/1‑150 (MQ=255)
                                                                                       atgatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGa                                                           <  2:651004/150‑1 (MQ=255)
                                                                                        tgatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGAt                                                          >  2:2372568/1‑150 (MQ=255)
                                                                                         gatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGAtt                                                         >  1:1692910/1‑150 (MQ=255)
                                                                                            aGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGa                                                      >  2:439653/1‑150 (MQ=255)
                                                                                            aGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGa                                                      <  2:4320143/150‑1 (MQ=255)
                                                                                             gTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGAc                                                     >  2:3760676/1‑150 (MQ=255)
                                                                                              tGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACa                                                    >  1:1604135/1‑150 (MQ=255)
                                                                                                   cAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATACACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATa                                               >  2:765417/1‑150 (MQ=255)
                                                                                                      gTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGa                                            <  2:3078130/150‑1 (MQ=255)
                                                                                                      gTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGa                                            <  2:1559383/150‑1 (MQ=255)
                                                                                                          cttcttTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGtt                                        >  1:3775238/1‑150 (MQ=255)
                                                                                                             cttTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTgcg                                     <  1:4344819/150‑1 (MQ=255)
                                                                                                              tttCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTgcgc                                    <  1:1102772/150‑1 (MQ=255)
                                                                                                              tttCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTgcgc                                    <  1:606472/150‑1 (MQ=255)
                                                                                                                tCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGt                                  <  2:538930/150‑1 (MQ=255)
                                                                                                                tCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGt                                  >  1:1171773/1‑150 (MQ=255)
                                                                                                                tCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGt                                  >  1:1171847/1‑150 (MQ=255)
                                                                                                                 cAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGAGTAGACAAAATAAGACGTTGcgcggt                                 >  1:45568/1‑148 (MQ=255)
                                                                                                                     tCGCCAAACTCGCGATCCAATTCACTCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATATGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTc                             >  1:2674254/1‑150 (MQ=255)
                                                                                                                     tCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTc                             >  1:356935/1‑150 (MQ=255)
                                                                                                                      cGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCa                            >  2:3526059/1‑150 (MQ=255)
                                                                                                                       gCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCAt                           <  1:4233524/150‑1 (MQ=255)
                                                                                                                         cAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCAttt                         >  1:3148541/1‑150 (MQ=255)
                                                                                                                         cAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCAttt                         >  1:3148469/1‑150 (MQ=255)
                                                                                                                          aaaCTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTc                        <  1:3964587/150‑1 (MQ=255)
                                                                                                                          aaaCTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTc                        >  2:4538492/1‑150 (MQ=255)
                                                                                                                             cTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAt                     <  2:1240823/150‑1 (MQ=255)
                                                                                                                              tCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAtt                    <  2:4531100/150‑1 (MQ=255)
                                                                                                                                  gATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAttgttg                >  2:140676/1‑150 (MQ=255)
                                                                                                                                    tCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGAc              <  2:3773271/150‑1 (MQ=255)
                                                                                                                                    tCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGAc              <  2:1213890/150‑1 (MQ=255)
                                                                                                                                     ccAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACt             <  1:2862229/150‑1 (MQ=255)
                                                                                                                                        aTTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCt          >  1:3692347/1‑150 (MQ=255)
                                                                                                                                          tCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGt        >  1:2141459/1‑150 (MQ=255)
                                                                                                                                            aCGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTAt      <  2:2074698/150‑1 (MQ=255)
                                                                                                                                            aCGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTAt      <  2:2953351/150‑1 (MQ=255)
                                                                                                                                            aCGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTAt      <  1:4417991/150‑1 (MQ=255)
                                                                                                                                               cATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCAc   >  2:3259339/1‑150 (MQ=255)
                                                                                                                                               cATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCAc   <  1:3440455/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAGGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACT  >  CP009273/2405799‑2406092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑