Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,412,205 C→A 90.6% D151Y (GAT→TAT)  yfcD ← putative NUDIX hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,412,2050CA90.6% 885.0 / 90.4 318D151Y (GAT→TAT) yfcDputative NUDIX hydrolase
Reads supporting (aligned to +/- strand):  ref base C (17/13);  new base A (141/147);  total (158/160)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

Only 100 of 318 total aligned reads displayed.

CTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCT  >  CP009273/2412058‑2412351
                                                                                                                                                   |                                                                                                                                                  
cTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGc                                                                                                                                                  >  1:437027/1‑150 (MQ=255)
 tCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCa                                                                                                                                                 >  2:1497063/1‑150 (MQ=255)
  cAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCTCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCATCATCCACAACGCTAGCGCTTTCAGCGATTATGGAGTGAACTCAAAGCAg                                                                                                                                                >  1:3655657/1‑150 (MQ=255)
  cAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAg                                                                                                                                                <  1:3767346/150‑1 (MQ=255)
      tGATGGGCTACCACTAAAAATGTTAATAGCCGCAATTGCGGCTCTTTTTTAATTCAGCCGTTTCAGTCTCAACGCCTTAATTTTAGGCATTTAGCTACATCCACAACACTACCGCTTTAAGCGAGACTTGAGTGAACACATAGCAgagag                                                                                                                                            >  1:1989435/1‑146 (MQ=255)
      tGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTg                                                                                                                                            <  1:2151661/150‑1 (MQ=255)
         tGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCgg                                                                                                                                         >  2:4538959/1‑150 (MQ=255)
         tGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCgg                                                                                                                                         >  1:4076198/1‑150 (MQ=255)
          gggCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGt                                                                                                                                        <  2:2319140/150‑1 (MQ=255)
          gggCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGt                                                                                                                                        <  1:1452179/150‑1 (MQ=255)
           ggCTACCACTAAAAATGTTAAGCGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTg                                                                                                                                       <  2:3875995/150‑1 (MQ=255)
           ggCTACCACTAAAAATGTTAAGCGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTg                                                                                                                                       <  1:934067/150‑1 (MQ=255)
              tACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGAtt                                                                                                                                    >  2:2122465/1‑150 (MQ=255)
                ccACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTGTCATTCTGCCGTTTCAGTCTCTACGGCTTCATGTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATttc                                                                                                                                  >  2:3783168/1‑150 (MQ=255)
                 cACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATttct                                                                                                                                 >  1:313459/1‑150 (MQ=255)
                  aCTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATttctt                                                                                                                                >  2:1710834/1‑150 (MQ=255)
                     aaaaaTGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCg                                                                                                                             <  1:1516027/150‑1 (MQ=255)
                     aaaaaTGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCg                                                                                                                             >  2:2248721/1‑150 (MQ=255)
                     aaaaaTGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCg                                                                                                                             <  1:3844854/150‑1 (MQ=255)
                      aaaaTGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCgg                                                                                                                            <  2:2025457/150‑1 (MQ=255)
                      aaaaTGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCgg                                                                                                                            >  1:262875/1‑150 (MQ=255)
                         aTGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACTCTAGCGCTTTAATAGAGTATGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTACGGCGt                                                                                                                         >  2:1028261/1‑150 (MQ=255)
                          tGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTc                                                                                                                        >  1:1099241/1‑150 (MQ=255)
                           gTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTca                                                                                                                       >  2:2342515/1‑150 (MQ=255)
                           gTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTca                                                                                                                       <  2:3370099/150‑1 (MQ=255)
                               agagCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTcagcca                                                                                                                   <  1:886914/150‑1 (MQ=255)
                                gagCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTcagccag                                                                                                                  >  1:2242913/1‑150 (MQ=255)
                                 agCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTcagccagc                                                                                                                 >  1:1258343/1‑150 (MQ=255)
                                   ccGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCaa                                                                                                               >  2:849704/1‑150 (MQ=255)
                                       aaTTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACtt                                                                                                           >  2:3761767/1‑150 (MQ=255)
                                        aTTGCGTCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCTCTTCATACACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTc                                                                                                          <  2:31283/150‑1 (MQ=255)
                                         ttGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCa                                                                                                         <  1:3467281/150‑1 (MQ=255)
                                           gCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACt                                                                                                       >  2:3347946/1‑150 (MQ=255)
                                              gCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGAc                                                                                                    >  2:2564453/1‑150 (MQ=255)
                                               ctctTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACt                                                                                                   <  2:2440347/150‑1 (MQ=255)
                                                tctTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACtt                                                                                                  >  2:2563750/1‑150 (MQ=255)
                                                  ttttttCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCa                                                                                                <  2:639120/150‑1 (MQ=255)
                                                      ttCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCtt                                                                                            >  2:166998/1‑150 (MQ=255)
                                                             tGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAg                                                                                     <  2:2159068/150‑1 (MQ=255)
                                                                cGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGc                                                                                  <  1:3381226/150‑1 (MQ=255)
                                                                      aGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAggg                                                                            >  1:3957249/1‑150 (MQ=255)
                                                                         ctctACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGAcc                                                                         <  1:885319/150‑1 (MQ=255)
                                                                          tctACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCg                                                                        <  1:4121278/150‑1 (MQ=255)
                                                                           ctACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGt                                                                       >  2:1619886/1‑150 (MQ=255)
                                                                            tACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTg                                                                      <  1:3115775/150‑1 (MQ=255)
                                                                             aCGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTga                                                                     <  1:1603044/150‑1 (MQ=255)
                                                                              cGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTgag                                                                    >  1:805167/1‑150 (MQ=255)
                                                                              cGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTgag                                                                    >  1:195816/1‑150 (MQ=255)
                                                                                   tCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACg                                                               >  2:163031/1‑150 (MQ=255)
                                                                                     aTTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCa                                                             >  1:1014225/1‑150 (MQ=255)
                                                                                       ttttGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGc                                                           >  1:210767/1‑150 (MQ=255)
                                                                                       ttttGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGc                                                           >  1:725225/1‑150 (MQ=255)
                                                                                             cATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACa                                                     >  1:2482740/1‑150 (MQ=255)
                                                                                              aTTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACaa                                                    >  1:2687781/1‑150 (MQ=255)
                                                                                                 gcgcTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATgc                                                 >  2:542616/1‑150 (MQ=255)
                                                                                                  cgcTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATgcg                                                >  1:1663759/1‑150 (MQ=255)
                                                                                                  cgcTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATgcg                                                >  2:1412426/1‑150 (MQ=255)
                                                                                                      tCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGcccc                                            >  2:2402191/1‑150 (MQ=255)
                                                                                                      tCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGcccc                                            >  1:2853047/1‑150 (MQ=255)
                                                                                                       cATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCa                                           <  2:116551/150‑1 (MQ=255)
                                                                                                        aTCCACAACGCTAGCTCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAg                                          >  1:3752267/1‑150 (MQ=255)
                                                                                                         tCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGa                                         >  2:475851/1‑150 (MQ=255)
                                                                                                          ccACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGac                                        <  2:2378331/150‑1 (MQ=255)
                                                                                                           cacaACGCTAGCGCTTTCAGAGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTAACTGACTTAATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCACCAGaca                                       >  2:3278737/1‑150 (MQ=255)
                                                                                                            acaACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGacac                                      >  1:2909383/1‑150 (MQ=255)
                                                                                                             caACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACg                                     <  2:2389381/150‑1 (MQ=255)
                                                                                                                cGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCa                                  >  1:2543273/1‑150 (MQ=255)
                                                                                                                cGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCa                                  >  1:1046116/1‑150 (MQ=255)
                                                                                                                 gCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCaa                                 >  2:1947602/1‑150 (MQ=255)
                                                                                                                 gCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCaa                                 <  2:3456311/150‑1 (MQ=255)
                                                                                                                    aGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAAttt                              <  2:4572004/150‑1 (MQ=255)
                                                                                                                    aGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAAttt                              <  1:1710834/150‑1 (MQ=255)
                                                                                                                    aGCGCTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAAttt                              >  1:2206922/1‑150 (MQ=255)
                                                                                                                       gcTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTa                           >  2:3675326/1‑150 (MQ=255)
                                                                                                                       gcTTTCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTa                           >  2:3934807/1‑150 (MQ=255)
                                                                                                                          ttCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCt                        <  1:248441/150‑1 (MQ=255)
                                                                                                                          ttCAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCt                        >  2:1115132/1‑150 (MQ=255)
                                                                                                                            cAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTc                      <  2:4496117/150‑1 (MQ=255)
                                                                                                                            cAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTc                      <  2:2990459/150‑1 (MQ=255)
                                                                                                                            cAGCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTc                      <  2:3196014/150‑1 (MQ=255)
                                                                                                                              gCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGa                    >  2:4009492/1‑150 (MQ=255)
                                                                                                                              gCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGa                    <  1:1786250/150‑1 (MQ=255)
                                                                                                                              gCGAGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGa                    <  2:1040208/150‑1 (MQ=255)
                                                                                                                                 aGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAAt                 <  1:2288659/150‑1 (MQ=255)
                                                                                                                                 aGTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAAt                 <  1:2387487/150‑1 (MQ=255)
                                                                                                                                  gTCTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAAATTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGTAATa                <  2:1077857/150‑1 (MQ=255)
                                                                                                                                    cTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGa              >  2:2860637/1‑150 (MQ=255)
                                                                                                                                    cTGGAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGa              >  2:4383315/1‑150 (MQ=255)
                                                                                                                                      ggAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAAc            <  1:1180437/150‑1 (MQ=255)
                                                                                                                                      ggAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAAc            <  1:2528626/150‑1 (MQ=255)
                                                                                                                                       gAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACt           <  1:4538959/150‑1 (MQ=255)
                                                                                                                                       gAGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACt           <  1:835814/150‑1 (MQ=255)
                                                                                                                                        aGTGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTg          >  2:1157614/1‑150 (MQ=255)
                                                                                                                                          tGAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGcc        <  2:2780964/150‑1 (MQ=255)
                                                                                                                                           gAACTCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGccc       >  2:3737543/1‑150 (MQ=255)
                                                                                                                                               tCATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGc   >  1:4612483/1‑150 (MQ=255)
                                                                                                                                                cATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGATACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCt  >  1:2934168/1‑150 (MQ=255)
                                                                                                                                                cATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCt  >  2:2491878/1‑150 (MQ=255)
                                                                                                                                                cATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCt  <  1:993604/150‑1 (MQ=255)
                                                                                                                                                cATAGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCt  >  1:3059344/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTACGGCTTCATTTTTGGCATTGCGCTTCATCCACAACGCTAGCGCTTTCAGCGAGTCTGGAGTGAACTCATCGCAGCGTGCGGTGATTTCTTCCGGCGTCAGCCAGCAAACTTCACTGACTTCATCTTCCTGTAGTGCGAAGGGACCGTGAGAGACGCAGCTGAACAATGCGCCCCAGACACGGCAATTTTTATCTTCGAAATAGAACTGCCCGTGCT  >  CP009273/2412058‑2412351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑