Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,421,032 G→T 7.5% S80* (TCA→TAA)  argT ← lysine/arginine/ornithine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,421,0320GT 7.5% 1148.2 / 36.7 309S80* (TCA→TAA) argTlysine/arginine/ornithine transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (157/127);  new base T (11/14);  total (168/141)
Fisher's exact test for biased strand distribution p-value = 3.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 312 total aligned reads displayed.

TCCAGCGTTGGCTGAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCG  >  CP009273/2420885‑2421181
                                                                                                                                                   |                                                                                                                                                     
tCCAGCGTTGGCTGAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAg                                                                                                                                                     >  2:3932046/1‑150 (MQ=255)
 ccAGCGTTGGCTGAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAgg                                                                                                                                                    <  2:665293/150‑1 (MQ=255)
   aGCGTTGGCTGAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTAGCTTTCAgggggggg                                                                                                                                                  >  2:1090476/1‑150 (MQ=255)
   aGCGTTGGCTGAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTAGCTTTCAGTGAgggg                                                                                                                                                  >  1:2861709/1‑150 (MQ=255)
     cGTTGGCTGAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGTGGGGAt                                                                                                                                                >  1:2670605/1‑150 (MQ=255)
      gTTGGCTGAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATc                                                                                                                                               <  2:3866783/150‑1 (MQ=255)
       ttGGCTGAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCa                                                                                                                                              <  2:2082283/150‑1 (MQ=255)
         ggCTGAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAgc                                                                                                                                            <  2:4628875/150‑1 (MQ=255)
         ggCTGAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAgc                                                                                                                                            <  1:3610714/150‑1 (MQ=255)
           cTGAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTAGCTTTCAGTGAGGGGATCAgcgc                                                                                                                                          >  1:3349387/1‑150 (MQ=255)
             gAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCTCTTTCAGTTAGGGGAACAGcgcgg                                                                                                                                        >  1:71886/1‑149 (MQ=255)
             gAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGt                                                                                                                                        >  1:3022289/1‑150 (MQ=255)
             gAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATAAGCGCGt                                                                                                                                        >  1:71513/1‑150 (MQ=255)
              aaTCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTc                                                                                                                                       <  2:232624/150‑1 (MQ=255)
               aTCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGTGGGGATCAGCGCGTCa                                                                                                                                      >  2:2974200/1‑150 (MQ=255)
                 cGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCaaa                                                                                                                                    <  2:560542/150‑1 (MQ=255)
                  ggTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAg                                                                                                                                   >  1:1269874/1‑150 (MQ=255)
                   gTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGt                                                                                                                                  >  2:1062563/1‑150 (MQ=255)
                    tGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTTAGGGGATCAGCGCGTCAAAGTc                                                                                                                                 <  2:1510320/150‑1 (MQ=255)
                    tGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTTAGGGGATCAGCGCGTCAAAGTc                                                                                                                                 <  2:4634977/150‑1 (MQ=255)
                       aCCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACt                                                                                                                              <  2:2578264/150‑1 (MQ=255)
                         cTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTgg                                                                                                                            >  2:1149045/1‑150 (MQ=255)
                         cTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTgg                                                                                                                            <  2:336630/150‑1 (MQ=255)
                           ttGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTTAGGGGATCAGCGCGTCAAAGTCACTGGCa                                                                                                                          <  1:3985047/150‑1 (MQ=255)
                               ccGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAAccc                                                                                                                      <  2:549371/150‑1 (MQ=255)
                               ccGCAATCAAACGAGAATATGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGTCGTTTATAGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTAGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAAccc                                                                                                                      <  2:4216827/150‑1 (MQ=255)
                                 gCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAg                                                                                                                    <  1:1977895/150‑1 (MQ=255)
                                 gCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAg                                                                                                                    <  1:1708052/150‑1 (MQ=255)
                                   aaTCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGt                                                                                                                  >  2:4199092/1‑150 (MQ=255)
                                      cAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACa                                                                                                               <  1:1538438/150‑1 (MQ=255)
                                       aaaCGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTAAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACaa                                                                                                              >  1:1282777/1‑149 (MQ=255)
                                        aaCGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTAGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACAtt                                                                                                             >  1:300369/1‑150 (MQ=255)
                                          cGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAATGTCACTGGCAACCCAGGTACATTTc                                                                                                           >  2:3443713/1‑150 (MQ=255)
                                           gagaATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCa                                                                                                          <  1:4620308/150‑1 (MQ=255)
                                            agaATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCAc                                                                                                         >  2:691155/1‑150 (MQ=255)
                                            agaATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCAc                                                                                                         <  1:1195390/150‑1 (MQ=255)
                                             gactCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTTAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCAcc                                                                                                        <  2:1205781/147‑1 (MQ=255)
                                              aaTCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCGTTAAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCAcca                                                                                                       >  2:3101609/1‑149 (MQ=255)
                                              aaTCTGCGGCGTACAGCTTGTCGGAGAAGGAAATCTCCTGCTTAAGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCt                                                                                                       <  1:1299718/150‑1 (MQ=255)
                                                         tACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTAGCTTTCAGTGAGGGGATCCGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTg                                                                                            >  2:1547638/1‑150 (MQ=255)
                                                            aGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTACTGGAAAGCGACGAAATAATAGCGTCGAGTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGcac                                                                                         >  2:3073045/1‑150 (MQ=255)
                                                              cTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACAt                                                                                       >  2:2618785/1‑150 (MQ=255)
                                                              cTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTAGCTTTAAATGAGGGGTTCAGCGCGTCAAAGTCACAGGCAACCCACGGACATTTAACCTGCATACCTTTTCACAt                                                                                       >  1:3974189/1‑150 (MQ=255)
                                                               ttGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTAGCTTTCAGTGGGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCAGCATCCGGTTGCACAtc                                                                                      >  1:1447113/1‑150 (MQ=255)
                                                                 gTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACAtctc                                                                                    <  2:1921578/150‑1 (MQ=255)
                                                                 gTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACAtctc                                                                                    <  1:87282/150‑1 (MQ=255)
                                                                 gTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACAactc                                                                                    >  2:4406573/1‑150 (MQ=255)
                                                                  tCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCg                                                                                   <  2:4253931/150‑1 (MQ=255)
                                                                   cGGAGATGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGt                                                                                  <  1:1109160/150‑1 (MQ=255)
                                                                    ggAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGGTCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGtt                                                                                 >  2:47683/1‑150 (MQ=255)
                                                                     gagaAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTTAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCAGCATCCGTTTGCACAAATCGTTa                                                                                >  1:3783332/1‑150 (MQ=255)
                                                                       gaAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAATTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTAcc                                                                              >  1:950525/1‑150 (MQ=255)
                                                                       gaAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGA‑TTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCg                                                                             <  2:1624038/150‑1 (MQ=255)
                                                                        aaGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCg                                                                             <  2:4009564/150‑1 (MQ=255)
                                                                            cAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCATTCAGTGAGGGGATCAGCGCGTCAAAGTCACAGGCAACCCAGGTACATTTCACCTGCAACCCTTTGCACAACACGTTAACGAGAt                                                                         >  1:4411982/1‑150 (MQ=255)
                                                                             aaTCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATc                                                                        <  1:3410836/150‑1 (MQ=255)
                                                                             aaTCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATc                                                                        <  1:1570832/150‑1 (MQ=255)
                                                                                   ctgctgACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATc                                                                  <  1:2021530/150‑1 (MQ=255)
                                                                                   ctgctgACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATc                                                                  >  2:4467633/1‑150 (MQ=255)
                                                                                      ctgACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACAACGCGTTACCGAGATCGATATCaaa                                                               >  2:3126920/1‑150 (MQ=255)
                                                                                           gTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACCTTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCcaa                                                          >  2:4355846/1‑150 (MQ=255)
                                                                                           gTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCCCGTCAAAGACACTTGCAACCCAGGTACATTTAAACAGCATCCGTTTGCACATCACCTTACCGAGATCGATATCAAACCcaa                                                          >  1:2995433/1‑150 (MQ=255)
                                                                                            tttATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCcaac                                                         <  1:4509875/150‑1 (MQ=255)
                                                                                             ttATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCccaca                                                        >  1:3425756/1‑150 (MQ=255)
                                                                                                    tAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCAc                                                 <  1:2769754/150‑1 (MQ=255)
                                                                                                     aaTGGAAAGCGACGAAATAATAGCGTCGATTTTTTTAGCTTTAAGTGAGGGGATCAGCGCGTCAAAGTCACTTGCAACCCAGGTAAATTTCACCTGCATCCGTTTGCACATCACGTTACCGAGATCGATATAAAAGCCAACAAAATCAcc                                                >  1:611269/1‑150 (MQ=255)
                                                                                                        ggAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTAACCTGCCTCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCgtt                                             >  1:1842864/1‑150 (MQ=255)
                                                                                                         gAAAGCGACGAAATAATAGCGTCGATTTTTTTAGCTTTCAGTGTGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGGTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTa                                            >  1:1418082/1‑150 (MQ=255)
                                                                                                         gAAAGCGACGAAATAATAGCGTCGATTTTTTTAGCGTTCAGTGAGGGGATCAACGCGTCAAAGTCAATGGCAAACCAGGTACAATTAACATGCAACCGTTTGCACAACTCGTTACCGAGATAGATATCAAAGCCAACAAAAACACCATTa                                            >  1:4253835/1‑150 (MQ=255)
                                                                                                          aaaGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCCACAAAATCACCTTTAg                                           >  2:3097806/1‑150 (MQ=255)
                                                                                                          aaaGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACATGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGGTACCGAGATCGATATAAAAGCCAACAAAATCACCTTTat                                           >  2:222869/1‑149 (MQ=255)
                                                                                                          aaaGCGACGAAATAATAGCGTCGATTTTTTTCGCGTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCAGCATCCGTTTGCACATCTCGTTACCGAGATAGATATCAAAGCCAACAAAATCACCTTTAg                                           >  2:1338681/1‑150 (MQ=255)
                                                                                                           aaGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCATTAGc                                          >  2:1752374/1‑150 (MQ=255)
                                                                                                           aaGCGACGAAATAATAGCGTCGATTTTTTTCGCGTTCAGGTAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTAAATTTCACCTGCATCCGTTTGCACATCACGGTACCGAGATCGAAATCAAAGCCAACAAAATCACCATTAGc                                          >  1:3776459/1‑150 (MQ=255)
                                                                                                               gacgaAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCt                                      <  2:1269903/150‑1 (MQ=255)
                                                                                                                acgaAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCtt                                     <  2:3181482/150‑1 (MQ=255)
                                                                                                                  gaAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTc                                   <  2:2766882/150‑1 (MQ=255)
                                                                                                                  gaAATAATAGCGTCGATTTTTTTCGCGTTCAGTGTGGGGATCAGCGCGTCAAATTCACTGGCAACCCAGGTACAATTAACCAGCAACCGTTTGCACATCTCGTTAACGAGATCGATATAAAAGCCAACAAAATAACCATTAGCaaattta                                   >  1:2454910/1‑144 (MQ=255)
                                                                                                                    aataatAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGa                                 <  1:565218/150‑1 (MQ=255)
                                                                                                                    aataatAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGa                                 <  2:4583123/150‑1 (MQ=255)
                                                                                                                    aataatAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGa                                 <  1:3534137/150‑1 (MQ=255)
                                                                                                                    aataatAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACAACACGTTACCGAGATCGATATCAAAGCCAACAAAATAACCTTTAGCATCTTTAGa                                 >  2:3511143/1‑150 (MQ=255)
                                                                                                                     ataataGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCAGCATCCGTTTTCACATCACGTTACCGAGCTCGATATCAAATCCAACAAAAAAACCGTTAGCATCTTTCGAt                                >  1:141576/1‑150 (MQ=255)
                                                                                                                     ataataGCGTCGATTTTTTTAGCGTTCAGTGAGGGGATCAGCGCGTAAAAGTCAATGGCAAACCAGGTACATTTAACCTGCATACGTTTTCACATCTCGTTACCGAGAACGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGAt                                >  2:3951615/1‑150 (MQ=255)
                                                                                                                       aataGCGTCGATTTTTTTCGCTTTCAGTGAGGGGCTCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATAGATATAAAAGCCAACAAAATCACCTTTAGCAACATTCGATga                              >  2:3247608/1‑150 (MQ=255)
                                                                                                                       aataGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATga                              <  2:183131/150‑1 (MQ=255)
                                                                                                                       aataGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTAAATTTCACCTGCATCCGTTTGCACATCTCGTTAACGAGATCGATATCAAAGCCAACAAAATAACCTTTAGCATCTTTCGATga                              >  2:1366477/1‑150 (MQ=255)
                                                                                                                         taGCGTCGATTTTTTTAGCTTTAAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTAAATTTCACCAGCATCCCGTTTCACAACTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCATTAGCATCTTTCGATgaga                            >  2:2702930/1‑150 (MQ=255)
                                                                                                                             gTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACgg                        >  1:2190580/1‑150 (MQ=255)
                                                                                                                              tCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGt                       <  2:3860867/150‑1 (MQ=255)
                                                                                                                                gATTTTTTTCGCTTTCAGTTAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGc                     <  1:4087100/150‑1 (MQ=255)
                                                                                                                                   ttttttCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTa                  >  2:3994296/1‑150 (MQ=255)
                                                                                                                                      tttCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAggt               <  2:3040696/150‑1 (MQ=255)
                                                                                                                                      tttCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAggt               <  1:4152715/150‑1 (MQ=255)
                                                                                                                                       ttCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAggtg              >  1:2400791/1‑150 (MQ=255)
                                                                                                                                        tCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAggtgg             >  2:3713177/1‑150 (MQ=255)
                                                                                                                                          gCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTa           <  1:326998/150‑1 (MQ=255)
                                                                                                                                           cTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTAt          >  2:4077960/1‑150 (MQ=255)
                                                                                                                                           cTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTAt          <  1:2655793/150‑1 (MQ=255)
                                                                                                                                               cAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGt      >  2:1939796/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TCCAGCGTTGGCTGAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCG  >  CP009273/2420885‑2421181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑