Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,442,971 C→A 61.5% E186D (GAG→GAT)  yfcO ← DUF2544 family putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,442,9710CA61.5% 113.6 / inf 270E186D (GAG→GAT) yfcODUF2544 family putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (57/47);  new base A (88/78);  total (145/125)
Fisher's exact test for biased strand distribution p-value = 8.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

TGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGA  >  CP009273/2442824‑2443120
                                                                                                                                                   |                                                                                                                                                     
tGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTtctc                                                                                                                                                     >  1:3102563/1‑150 (MQ=255)
 ggCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCa                                                                                                                                                    >  2:4191761/1‑150 (MQ=255)
 ggCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCa                                                                                                                                                    >  2:691954/1‑150 (MQ=255)
         cGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACAcc                                                                                                                                            >  1:4038673/1‑150 (MQ=255)
         cGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACAcc                                                                                                                                            >  1:3800069/1‑150 (MQ=255)
         cGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACAcc                                                                                                                                            <  2:3194403/150‑1 (MQ=255)
          gagaCTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCg                                                                                                                                           >  1:2267414/1‑150 (MQ=255)
          gagaCTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCg                                                                                                                                           >  1:3115287/1‑150 (MQ=255)
          gagaCTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCg                                                                                                                                           <  1:1803792/150‑1 (MQ=255)
           agaCTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGc                                                                                                                                          >  2:3823991/1‑150 (MQ=255)
             aCTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAAACCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGccc                                                                                                                                        >  2:1548921/1‑150 (MQ=255)
              cTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCg                                                                                                                                       >  2:4454186/1‑150 (MQ=255)
               ttCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGt                                                                                                                                      >  2:413034/1‑150 (MQ=255)
               ttCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGt                                                                                                                                      >  1:4308430/1‑150 (MQ=255)
               ttCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGt                                                                                                                                      <  1:1370514/150‑1 (MQ=255)
                tCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTc                                                                                                                                     <  1:2018992/150‑1 (MQ=255)
                 ccGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGtag                                                                                                                                    <  1:4323005/150‑3 (MQ=255)
                 ccGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCg                                                                                                                                    >  2:3129776/1‑150 (MQ=255)
                    ccTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACt                                                                                                                                 <  1:4558028/150‑1 (MQ=255)
                     cTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTg                                                                                                                                <  2:722100/150‑1 (MQ=255)
                       tGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCa                                                                                                                              >  2:4486322/1‑150 (MQ=255)
                        gTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAg                                                                                                                             <  2:3578461/150‑1 (MQ=255)
                        gTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAg                                                                                                                             <  1:2035035/150‑1 (MQ=255)
                          cacaTCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAggg                                                                                                                           <  1:344611/150‑1 (MQ=255)
                           acaTCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGt                                                                                                                          >  1:3678197/1‑150 (MQ=255)
                              tCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACAACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAg                                                                                                                       >  1:1203494/1‑150 (MQ=255)
                                 cGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAg                                                                                                                    <  1:1007836/150‑1 (MQ=255)
                                    cgcgGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGttt                                                                                                                 <  1:2150472/150‑1 (MQ=255)
                                     gcgGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGAGTACTGACGCACAACTTCCCCTCTTTAATCAAATACGATGAAAGCGTTTTATCAACAACACCGCCCGTCGACTGCAGGGTGAGCAGtttt                                                                                                                >  2:4027803/1‑150 (MQ=255)
                                      cgGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTg                                                                                                               >  2:2533409/1‑150 (MQ=255)
                                      cgGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTg                                                                                                               >  1:641663/1‑150 (MQ=255)
                                            aaGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTAc                                                                                                         <  1:2058489/150‑1 (MQ=255)
                                            aaGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTAc                                                                                                         >  1:3785966/1‑150 (MQ=255)
                                             aGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACt                                                                                                        >  2:4527451/1‑150 (MQ=255)
                                               ttATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGa                                                                                                      >  1:2962597/1‑150 (MQ=255)
                                               ttATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGa                                                                                                      <  2:444177/150‑1 (MQ=255)
                                               ttATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGa                                                                                                      <  1:3644890/150‑1 (MQ=255)
                                                 atcatcTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACa                                                                                                    <  2:4042991/150‑1 (MQ=255)
                                                      cTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAAtt                                                                                               >  1:2653613/1‑150 (MQ=255)
                                                      cTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAAtt                                                                                               >  1:3990818/1‑150 (MQ=255)
                                                          gCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCa                                                                                           >  1:3251631/1‑150 (MQ=255)
                                                           cAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCAt                                                                                          >  1:2452423/1‑150 (MQ=255)
                                                             aCCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTc                                                                                        >  2:266713/1‑150 (MQ=255)
                                                              cccAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTcc                                                                                       <  2:3955969/150‑1 (MQ=255)
                                                                cAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCAt                                                                                     <  2:1860792/150‑1 (MQ=255)
                                                                cAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCAt                                                                                     >  2:2939588/1‑150 (MQ=255)
                                                                 aGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATg                                                                                    <  1:3245740/150‑1 (MQ=255)
                                                                  gCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGa                                                                                   <  1:508042/150‑1 (MQ=255)
                                                                   cGGCAGTAACCTCCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCATGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGaa                                                                                  <  2:2405826/150‑1 (MQ=255)
                                                                       aGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGt                                                                              >  2:1676010/1‑150 (MQ=255)
                                                                         tAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCa                                                                            <  1:3759755/150‑1 (MQ=255)
                                                                         tAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCa                                                                            >  2:1123471/1‑150 (MQ=255)
                                                                          aaCCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCAt                                                                           <  2:1377977/150‑1 (MQ=255)
                                                                           accaccTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATa                                                                          <  1:3129776/150‑1 (MQ=255)
                                                                            ccaccTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATaa                                                                         <  1:1433265/150‑1 (MQ=255)
                                                                             caccTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAg                                                                        <  1:1024630/150‑1 (MQ=255)
                                                                             caccTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAg                                                                        >  1:3717978/1‑150 (MQ=255)
                                                                                 tGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTg                                                                    <  1:1107572/150‑1 (MQ=255)
                                                                                   tttCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGaa                                                                  >  1:1615671/1‑150 (MQ=255)
                                                                                   tttCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGaa                                                                  <  1:75373/150‑1 (MQ=255)
                                                                                     tcattcatCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACa                                                                <  2:3260083/150‑1 (MQ=255)
                                                                                     tcattcatCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACa                                                                <  1:1963253/150‑1 (MQ=255)
                                                                                          catCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAAc                                                           <  1:4393158/150‑1 (MQ=255)
                                                                                          catCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAAc                                                           >  2:3589396/1‑150 (MQ=255)
                                                                                              tGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGAt                                                       >  1:960643/1‑150 (MQ=255)
                                                                                              tGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGAt                                                       >  1:715822/1‑150 (MQ=255)
                                                                                                 gTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGa                                                    <  1:2489299/150‑1 (MQ=255)
                                                                                                    cTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACaa                                                 >  2:1325605/1‑150 (MQ=255)
                                                                                                    cTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACaa                                                 >  1:1285781/1‑150 (MQ=255)
                                                                                                      gTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAAt                                               <  2:285068/150‑1 (MQ=255)
                                                                                                      gTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAAt                                               >  2:915982/1‑150 (MQ=255)
                                                                                                       tCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAAtc                                              <  2:4012975/150‑1 (MQ=255)
                                                                                                       tCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAAtc                                              <  2:3339291/150‑1 (MQ=255)
                                                                                                        cGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAAtcg                                             <  2:270901/150‑1 (MQ=255)
                                                                                                          cacaACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAAtcgtg                                           <  1:4621086/150‑1 (MQ=255)
                                                                                                          cacaACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAAtcgtg                                           >  1:2384696/1‑150 (MQ=255)
                                                                                                           acaACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAAtcgtgt                                          <  1:864939/150‑1 (MQ=255)
                                                                                                              aCTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAAtcgtgtcgt                                       >  2:2929085/1‑150 (MQ=255)
                                                                                                               cTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAAtcgtgtcgtg                                      <  2:1643908/150‑1 (MQ=255)
                                                                                                               cTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAAtcgtgtcgtg                                      >  2:1041092/1‑150 (MQ=255)
                                                                                                               cTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCAGTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAAtcgtgtcgtg                                      <  2:3171697/150‑1 (MQ=255)
                                                                                                                ttCCCATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGa                                     >  2:2255873/1‑150 (MQ=255)
                                                                                                                    cATCTTTCATCAAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTAt                                 >  2:2089847/1‑150 (MQ=255)
                                                                                                                            atcaAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGcccc                         >  1:3896526/1‑150 (MQ=255)
                                                                                                                            atcaAATACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGcccc                         >  1:3287686/1‑150 (MQ=255)
                                                                                                                               aaaTACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGa                      >  2:944523/1‑150 (MQ=255)
                                                                                                                               aaaTACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGa                      >  1:2787004/1‑150 (MQ=255)
                                                                                                                                aaTACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGAt                     >  1:1719935/1‑150 (MQ=255)
                                                                                                                                  tACGTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATAc                   <  2:2631596/150‑1 (MQ=255)
                                                                                                                                   aCGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACg                  <  2:4366041/150‑1 (MQ=255)
                                                                                                                                    cGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGt                 >  1:1499835/1‑150 (MQ=255)
                                                                                                                                     gTTGAAAGCGTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTc                >  1:4206005/1‑150 (MQ=255)
                                                                                                                                       tGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCAc              <  1:201879/150‑1 (MQ=255)
                                                                                                                                       tGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCAc              <  1:4507683/150‑1 (MQ=255)
                                                                                                                                        gAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACg             >  1:1231323/1‑150 (MQ=255)
                                                                                                                                              gTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCt       <  2:3813674/150‑1 (MQ=255)
                                                                                                                                              gTTTTATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGcccccg       >  1:1855057/1‑149 (MQ=255)
                                                                                                                                               ttttCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTg      <  1:4428307/150‑1 (MQ=255)
                                                                                                                                               ttttATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTg      >  1:4061395/1‑150 (MQ=255)
                                                                                                                                               ttttATCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTg      <  2:2748833/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGA  >  CP009273/2442824‑2443120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑