Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,456,508 A→T 17.6% S6S (TCA→TCT)  yfdF → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,456,5080AT17.6% 625.7 / 115.5 233S6S (TCA→TCT) yfdFuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (93/99);  new base T (16/25);  total (109/124)
Fisher's exact test for biased strand distribution p-value = 3.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 238 total aligned reads displayed.

CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAA  >  CP009273/2456363‑2456657
                                                                                                                                                 |                                                                                                                                                     
 cccccTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTtcaatcaa                                                                                                                                                  <  1:2469858/150‑1 (MQ=255)
     cTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAAt                                                                                                                                              >  1:1047415/1‑150 (MQ=255)
       ggCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATAc                                                                                                                                            >  1:3685879/1‑150 (MQ=255)
         cTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCa                                                                                                                                          >  1:3812008/1‑150 (MQ=255)
              gtgCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCa                                                                                                                                     >  1:294176/1‑150 (MQ=255)
                 cgcTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCt                                                                                                                                  <  1:11544/150‑1 (MQ=255)
                   cTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTa                                                                                                                                >  2:1779962/1‑150 (MQ=255)
                       tACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCa                                                                                                                            <  2:943948/150‑1 (MQ=255)
                         cacTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGa                                                                                                                          >  2:333355/1‑150 (MQ=255)
                              aaaTTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCa                                                                                                                     >  1:1387446/1‑150 (MQ=255)
                              aaaTTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCa                                                                                                                     >  1:645402/1‑150 (MQ=255)
                               aaTTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCAt                                                                                                                    >  2:4543168/1‑150 (MQ=255)
                                aTTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATc                                                                                                                   <  2:3916469/150‑1 (MQ=255)
                                aTTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATc                                                                                                                   <  2:3593935/150‑1 (MQ=255)
                                   aaaGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAAt                                                                                                                >  1:1613615/1‑150 (MQ=255)
                                   aaaGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAAt                                                                                                                >  2:3710406/1‑150 (MQ=255)
                                   aaaGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAAt                                                                                                                <  1:3083101/150‑1 (MQ=255)
                                     aGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGa                                                                                                              >  2:920165/1‑150 (MQ=255)
                                     aGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGa                                                                                                              >  1:2685091/1‑150 (MQ=255)
                                     aGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGa                                                                                                              <  2:2397209/150‑1 (MQ=255)
                                     aGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGa                                                                                                              >  1:3251098/1‑150 (MQ=255)
                                         aGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAAt                                                                                                          >  1:4575931/1‑150 (MQ=255)
                                          ggTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATa                                                                                                         <  1:733471/150‑1 (MQ=255)
                                          ggTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATa                                                                                                         <  2:2194954/150‑1 (MQ=255)
                                            ttCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAAc                                                                                                       >  1:2827202/1‑150 (MQ=255)
                                             tCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACa                                                                                                      <  2:4157432/150‑1 (MQ=255)
                                              cTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACaa                                                                                                     <  2:405996/150‑1 (MQ=255)
                                               taaataaaTGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAat                                                                                                    <  1:986813/150‑1 (MQ=255)
                                               taaataaaTGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAat                                                                                                    <  1:44935/150‑1 (MQ=255)
                                                aaataaatGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACActg                                                                                                   <  2:2165029/150‑1 (MQ=255)
                                                aaataaatGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAatg                                                                                                   >  2:3454768/1‑150 (MQ=255)
                                                aaataaatGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAatg                                                                                                   >  2:2521998/1‑150 (MQ=255)
                                                aaataaatGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAatg                                                                                                   <  2:1347460/150‑1 (MQ=255)
                                                   taaatGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAatgatg                                                                                                >  2:3388952/1‑150 (MQ=255)
                                                   taaatGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAatgatg                                                                                                >  2:3168926/1‑150 (MQ=255)
                                                     aatGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGaa                                                                                              <  2:3685879/150‑1 (MQ=255)
                                                     aatGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGaa                                                                                              <  1:3976428/150‑1 (MQ=255)
                                                     aatGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGaa                                                                                              <  2:3025759/150‑1 (MQ=255)
                                                     aatGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGaa                                                                                              <  2:710301/150‑1 (MQ=255)
                                                         aGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTa                                                                                          <  1:2798688/150‑1 (MQ=255)
                                                          gCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTaa                                                                                         <  1:1954789/150‑1 (MQ=255)
                                                             ttttttGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATgg                                                                                      <  2:654988/150‑1 (MQ=255)
                                                               ttttGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGAt                                                                                    <  2:826505/150‑1 (MQ=255)
                                                                tttGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGAtt                                                                                   <  2:2404820/150‑1 (MQ=255)
                                                                  tGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAg                                                                                 <  2:1545371/150‑1 (MQ=255)
                                                                  tGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAg                                                                                 <  2:518852/150‑1 (MQ=255)
                                                                      aGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACa                                                                             >  1:4449412/1‑150 (MQ=255)
                                                                      aGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACa                                                                             >  1:3553839/1‑150 (MQ=255)
                                                                      aGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGACGAAGTTAATGGATTAGTACa                                                                             >  2:1463844/1‑150 (MQ=255)
                                                                       gTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACaa                                                                            <  1:1222885/150‑1 (MQ=255)
                                                                       gTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACaa                                                                            >  1:3291825/1‑150 (MQ=255)
                                                                        tCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAag                                                                           >  2:761929/1‑150 (MQ=255)
                                                                        tCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAag                                                                           <  2:1988737/150‑1 (MQ=255)
                                                                          tATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAagag                                                                         <  2:427647/150‑1 (MQ=255)
                                                                           aTTTCATGAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGt                                                                        <  2:1227024/150‑1 (MQ=255)
                                                                            tttCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGtt                                                                       <  2:2944710/150‑1 (MQ=255)
                                                                               cATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCaa                                                                    >  2:3004948/1‑150 (MQ=255)
                                                                                   aGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAAc                                                                >  1:3506890/1‑150 (MQ=255)
                                                                                       aaTATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCttt                                                            >  1:4099059/1‑150 (MQ=255)
                                                                                           tatatTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTaa                                                        <  1:871823/150‑1 (MQ=255)
                                                                                             tatTTGTAACTAATCTATCAAAATGGAATATAATCATGCTACCATCTATTTCTATCAACATTACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTATTGGATTAGTACAATAGTTCAAAAACCTTTTTAATg                                                      <  1:4540737/150‑1 (MQ=255)
                                                                                             tatTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATg                                                      >  1:823486/1‑150 (MQ=255)
                                                                                              atTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCATCGCAGATTACCCAGAATACATAAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAAAGTTCAAAAACCTTTTTAAttg                                                     >  2:3500267/1‑148 (MQ=255)
                                                                                                  gTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTaag                                                 <  1:730257/150‑1 (MQ=255)
                                                                                                   tAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTaagg                                                <  1:1385826/150‑1 (MQ=255)
                                                                                                   tAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTaagg                                                >  2:3449717/1‑150 (MQ=255)
                                                                                                   tAAAAAATCAATCAAAATGGAATAAAATCATGCTAACATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTaagg                                                <  1:191954/150‑1 (MQ=255)
                                                                                                     aCAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTaaggaa                                              >  1:256566/1‑150 (MQ=255)
                                                                                                          tcaatcaaAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAAt                                         <  2:1201287/150‑1 (MQ=255)
                                                                                                           caatcaaAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATa                                        >  1:3673267/1‑150 (MQ=255)
                                                                                                            aatcaaAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATaa                                       >  1:4070054/1‑150 (MQ=255)
                                                                                                             atcaaAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAg                                      <  2:1255343/150‑1 (MQ=255)
                                                                                                               caaAATGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCa                                    >  1:4166107/1‑150 (MQ=255)
                                                                                                                aaaaTGGAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCAc                                   >  1:213989/1‑150 (MQ=255)
                                                                                                                aaaaTGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCAc                                   >  1:1710662/1‑150 (MQ=255)
                                                                                                                 aaaTGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCAcc                                  <  2:4259410/150‑1 (MQ=255)
                                                                                                                   aTGGAATAAAATCATGCTACCCTCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTg                                <  2:33018/150‑1 (MQ=255)
                                                                                                                    tGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGt                               >  1:1195181/1‑150 (MQ=255)
                                                                                                                    tGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGt                               >  2:3278504/1‑150 (MQ=255)
                                                                                                                     ggAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTa                              >  2:2087419/1‑150 (MQ=255)
                                                                                                                      gAATAAAATCATGCTACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTAt                             >  1:1699543/1‑150 (MQ=255)
                                                                                                                      gAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTAt                             <  1:4115574/150‑1 (MQ=255)
                                                                                                                       aaTAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTAtt                            >  2:4335385/1‑150 (MQ=255)
                                                                                                                            aaTCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACa                       <  1:3424225/150‑1 (MQ=255)
                                                                                                                                  gCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACt                 >  1:3193387/1‑150 (MQ=255)
                                                                                                                                   cTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACtt                <  1:297719/150‑1 (MQ=255)
                                                                                                                                   cTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACtt                <  1:3415513/150‑1 (MQ=255)
                                                                                                                                    tACCATCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTg               <  2:3942934/150‑1 (MQ=255)
                                                                                                                                    tACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTg               <  2:2852868/150‑1 (MQ=255)
                                                                                                                                    tACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGACGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTg               <  1:1463844/150‑1 (MQ=255)
                                                                                                                                    tACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCACCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTg               <  1:312588/150‑1 (MQ=255)
                                                                                                                                      ccATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAg             >  1:2422762/1‑150 (MQ=255)
                                                                                                                                       cATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGc            <  2:2995732/150‑1 (MQ=255)
                                                                                                                                        aTCTATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCt           <  1:1526470/150‑1 (MQ=255)
                                                                                                                                        aTCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCt           <  1:2288226/150‑1 (MQ=255)
                                                                                                                                           tATTTCTATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTtat        <  2:1099781/150‑1 (MQ=255)
                                                                                                                                            aTTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTtata       <  1:2538088/150‑1 (MQ=255)
                                                                                                                                              ttCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATaaa     <  1:3388901/150‑1 (MQ=255)
                                                                                                                                              ttCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATaaa     <  1:3168926/150‑1 (MQ=255)
                                                                                                                                                caatcaaCAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATaaaaa   >  1:79631/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAA  >  CP009273/2456363‑2456657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑