BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,457,130	C→A	5.4%	R214S (CGT→AGT)	yfdF →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,457,130	0	C	A	5.4%	1084.2 / 23.4	282	R214S (CGT→AGT)	yfdF	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (128/138); new base A (7/8); total (135/147)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

Only 100 of 285 total aligned reads displayed.

GGAAAAGCGAACCAACGGACTTATTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACGGGGAAAGACTG  >  CP009273/2456981‑2457279

   aaaGCGAACCAACGGACTTATTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTa                                                                                                                                                    >  1:3626788/1‑150 (MQ=255)

     aGCGAACCACCGGACTTATTTACCTGGTACGGTAAATATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTAtt                                                                                                                                                  <  1:1653761/150‑1 (MQ=255)

        gAACCACCGGACTTATTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAAt                                                                                                                                               <  1:13265/150‑1 (MQ=255)

        gAACCAACGGACTTATTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAAt                                                                                                                                               >  2:1598306/1‑150 (MQ=255)

           ccAACGGACTTATTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGt                                                                                                                                            >  1:3020132/1‑150 (MQ=255)

              aCGGACTTATTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGAt                                                                                                                                         <  2:2722372/150‑1 (MQ=255)

               cGGACTTATTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTtata                                                                                                                                        >  2:2871844/1‑150 (MQ=255)

                   cTTATTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTAGTATTAATGGTGATAGtct                                                                                                                                    <  1:4119092/150‑1 (MQ=255)

                    ttATTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGtctc                                                                                                                                   >  2:1387625/1‑150 (MQ=255)

                          aCCTGGTACGTTAAATATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCaa                                                                                                                             <  1:2858359/150‑1 (MQ=255)

                             tGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAatat                                                                                                                          <  2:2061309/150‑1 (MQ=255)

                             tGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAatat                                                                                                                          <  1:3369999/150‑1 (MQ=255)

                                    gTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTg                                                                                                                   >  2:2362547/1‑150 (MQ=255)

                                     tAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGa                                                                                                                  >  1:4643283/1‑150 (MQ=255)

                                      aaaGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGaa                                                                                                                 <  1:4341778/150‑1 (MQ=255)

                                      aaaGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGaa                                                                                                                 <  1:2372629/150‑1 (MQ=255)

                                        aGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGaaaa                                                                                                               <  1:1986917/150‑1 (MQ=255)

                                         gATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAAc                                                                                                              >  1:2721267/1‑150 (MQ=255)

                                           tAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATAGTAGTGAAAACGc                                                                                                            >  1:2446150/1‑150 (MQ=255)

                                            aaaaaaGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGcc                                                                                                           <  2:59861/150‑1 (MQ=255)

                                            aaaaaaGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGcc                                                                                                           >  1:4328336/1‑150 (MQ=255)

                                               aaaGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAgg                                                                                                        >  2:1929464/1‑150 (MQ=255)

                                               aaaGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAgg                                                                                                        >  2:4507331/1‑150 (MQ=255)

                                               aaaGGCGACTCCCTCGATATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTATGGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAgg                                                                                                        <  1:3128770/150‑1 (MQ=255)

                                                  ggCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGt                                                                                                     >  1:3970685/1‑150 (MQ=255)

                                                   gCGACTCTCTCGCTATTGTTTTTAAAAATAAAACCGGAAATGATTACTTATCTATCGGTTACTACGATCATGACGACTACCACATTCAAAGAGGAATTCGTTTTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTg                                                                                                    <  2:1501401/150‑1 (MQ=255)

                                                        tctctcGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCa                                                                                               <  1:4061668/150‑1 (MQ=255)

                                                         ctctcGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAg                                                                                              <  2:1217954/150‑1 (MQ=255)

                                                         ctctcGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAg                                                                                              >  1:2332633/1‑150 (MQ=255)

                                                         ctctcGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAg                                                                                              <  2:3077401/150‑1 (MQ=255)

                                                          tctcGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGc                                                                                             >  2:351966/1‑150 (MQ=255)

                                                            ccGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGTAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGt                                                                                           <  1:1278372/149‑1 (MQ=255)

                                                               cTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGt                                                                                        >  2:144438/1‑150 (MQ=255)

                                                                tATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGTAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGtt                                                                                       <  1:2185369/150‑1 (MQ=255)

                                                                tATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGtt                                                                                       >  2:4379340/1‑150 (MQ=255)

                                                                  ttGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTg                                                                                     <  2:2951028/150‑1 (MQ=255)

                                                                  ttGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTg                                                                                     <  2:2951103/150‑1 (MQ=255)

                                                                   tGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGa                                                                                    >  2:67238/1‑150 (MQ=255)

                                                                   tGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGa                                                                                    >  1:2469908/1‑150 (MQ=255)

                                                                      aaTTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAg                                                                                 >  2:2686262/1‑150 (MQ=255)

                                                                         taaaaataaaaaCGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAg                                                                              >  1:3115606/1‑150 (MQ=255)

                                                                          aaaaaaaaaaaCGTAAATGATTACTTATCTCTCGTTTACTACGATCAGGACGACTACAACATTCAAAGAGTAATTAGTATTAATGGTGATAGTCACACCCAATATTGTAGTTAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGt                                                                             <  1:3560208/150‑1 (MQ=255)

                                                                           aaaataaaaaCGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTAGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTa                                                                            >  2:1840407/1‑150 (MQ=255)

                                                                            aaataaaaaCGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTaa                                                                           <  2:1718515/150‑1 (MQ=255)

                                                                            aaataaaaaCGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTaa                                                                           >  2:334923/1‑150 (MQ=255)

                                                                              ataaaaaCGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAg                                                                         >  1:766829/1‑150 (MQ=255)

                                                                               taaaaaCGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGc                                                                        >  1:3886838/1‑150 (MQ=255)

                                                                               taaaaaCGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGc                                                                        >  2:747553/1‑150 (MQ=255)

                                                                                aaaaaCGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCt                                                                       >  1:1535661/1‑150 (MQ=255)

                                                                                    aCGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCa                                                                   <  1:1621972/150‑1 (MQ=255)

                                                                                     cGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCAt                                                                  <  2:2031054/150‑1 (MQ=255)

                                                                                     cGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCAt                                                                  <  2:2436761/150‑1 (MQ=255)

                                                                                       gAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATgg                                                                >  2:846011/1‑150 (MQ=255)

                                                                                        aaaTGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGc                                                               >  2:3406538/1‑150 (MQ=255)

                                                                                         aaTGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCa                                                              <  2:1353128/150‑1 (MQ=255)

                                                                                           tGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGa                                                            <  2:2792651/150‑1 (MQ=255)

                                                                                             aTTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAAt                                                          <  1:961549/150‑1 (MQ=255)

                                                                                              ttACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATc                                                         >  2:3068145/1‑150 (MQ=255)

                                                                                                aCTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCAt                                                       >  2:2032202/1‑150 (MQ=255)

                                                                                                aCTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCAt                                                       >  1:1102991/1‑150 (MQ=255)

                                                                                                aCTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCAt                                                       <  1:1724845/150‑1 (MQ=255)

                                                                                                  ttATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCAttt                                                     <  1:19106/150‑1 (MQ=255)

                                                                                                  ttATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCAttt                                                     <  1:3337627/150‑1 (MQ=255)

                                                                                                    aTCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGc                                                   >  1:1219682/1‑150 (MQ=255)

                                                                                                     tctctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCa                                                  >  2:1190549/1‑150 (MQ=255)

                                                                                                     tctctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCa                                                  >  2:3696810/1‑150 (MQ=255)

                                                                                                      ctctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCaa                                                 >  1:1388266/1‑150 (MQ=255)

                                                                                                       tctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAAc                                                >  1:1432805/1‑150 (MQ=255)

                                                                                                          cGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTgg                                             >  2:460/1‑150 (MQ=255)

                                                                                                          cGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTgg                                             >  2:398/1‑150 (MQ=255)

                                                                                                            gTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGtt                                           <  1:4188891/150‑1 (MQ=255)

                                                                                                                  acGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGatc                                     >  1:4345894/1‑150 (MQ=255)

                                                                                                                   cGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGatca                                    <  1:1479132/150‑1 (MQ=255)

                                                                                                                    gATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGatcat                                   >  1:3228468/1‑150 (MQ=255)

                                                                                                                      tccggACGACTACCACATATATTGTTTAATTTTTATTAATTTTTATAGTCTCACCCAATTTTTTTGTGAAAACGACAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGAtcatca                                 <  1:180182/147‑1 (MQ=255)

                                                                                                                        aGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAgg                               >  2:3481035/1‑150 (MQ=255)

                                                                                                                          gacgacTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGtt                             >  1:3650955/1‑150 (MQ=255)

                                                                                                                          gacgacTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGtt                             <  2:1777395/150‑1 (MQ=255)

                                                                                                                          gacgacTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGtt                             <  1:560301/150‑1 (MQ=255)

                                                                                                                           acgacTACCACATTCATAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCATGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTg                            <  1:3477697/150‑1 (MQ=255)

                                                                                                                            cgacTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGt                           >  2:2584647/1‑150 (MQ=255)

                                                                                                                               cTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTaaa                        >  1:1060398/1‑150 (MQ=255)

                                                                                                                                tACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAAc                       <  2:3953275/150‑1 (MQ=255)

                                                                                                                                 aCCCCATTCAACGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACg                      <  1:3920734/150‑1 (MQ=255)

                                                                                                                                    acaTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGc                   <  1:87277/150‑1 (MQ=255)

                                                                                                                                    acaTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGc                   <  1:2968754/150‑1 (MQ=255)

                                                                                                                                    acaTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGc                   >  2:412/1‑150 (MQ=255)

                                                                                                                                     caTTCAACGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCt                  <  1:1598306/150‑1 (MQ=255)

                                                                                                                                     caTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCt                  <  2:2532540/150‑1 (MQ=255)

                                                                                                                                     caTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCt                  <  1:677858/150‑1 (MQ=255)

                                                                                                                                      aTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTc                 <  2:1314868/150‑1 (MQ=255)

                                                                                                                                          aaaGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACg             <  1:4011749/150‑1 (MQ=255)

                                                                                                                                             gagGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACgggg          <  1:4379340/150‑1 (MQ=255)

                                                                                                                                              agGAATTAGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACGGGGa         >  2:1035065/1‑150 (MQ=255)

                                                                                                                                               ggAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACGGGGaa        <  2:700496/150‑1 (MQ=255)

                                                                                                                                               ggAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACGGGGaa        >  1:2580027/1‑150 (MQ=255)

                                                                                                                                                  aTTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACGGGGAAAGa     >  1:183022/1‑150 (MQ=255)

                                                                                                                                                  aTTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACGGGGAAAGa     >  1:3610016/1‑150 (MQ=255)

                                                                                                                                                   ttCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACGGGGAAAGAc    <  2:2448317/150‑1 (MQ=255)

                                                                                                                                                     cGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACGGGGAAAGACTg  <  2:2519981/150‑1 (MQ=255)

GGAAAAGCGAACCAACGGACTTATTTACCTGGTACGGTAAAGATAAAAAAGGCGACTCTCTCGCTATTGTAATTAAAAATAAAAACGGAAATGATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTAAACGAGCTCAACGGGGAAAGACTG  >  CP009273/2456981‑2457279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`