Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,498,349 C→A 5.5% A226A (GCG→GCT)  ypdE ← aminopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,498,3490CA 5.5% 1159.7 / 25.9 298A226A (GCG→GCT) ypdEaminopeptidase
Reads supporting (aligned to +/- strand):  ref base C (136/145);  new base A (5/12);  total (141/157)
Fisher's exact test for biased strand distribution p-value = 1.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 299 total aligned reads displayed.

TCTGCCTGCAACGGCACGCCAATTTCTGCCGCCACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTA  >  CP009273/2498200‑2498497
                                                                                                                                                     |                                                                                                                                                    
   gcctgcAACGGCACGCCAATTTCTGCCGCCACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGcc                                                                                                                                                   <  2:3696394/150‑1 (MQ=255)
    cctgcAACGGCACGCCAATTTCTGCCGCCACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGccc                                                                                                                                                  <  1:2188282/150‑1 (MQ=255)
     ctgcAACGGCACGCCAATTTCTGCCGCCACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCca                                                                                                                                                 <  1:1049040/150‑1 (MQ=255)
     ctgcAACGGCACGCCAATTTCTGCCGCCACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCca                                                                                                                                                 <  1:239175/150‑1 (MQ=255)
         aaCGGCACGCCAATTTCTGCCGCCACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCcagcag                                                                                                                                             >  2:2066501/1‑150 (MQ=255)
          aCGGCACGCCAATTTCTGCCGCCACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAgg                                                                                                                                            <  2:3090394/150‑1 (MQ=255)
          aCGGCACGCCAATTTCTGCCGCCACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAgg                                                                                                                                            <  1:2247144/150‑1 (MQ=255)
           cGGCACGCCAATTTCTGCCGCCACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTTGCCGCGCCATAATCAAAGTTTTTAGCCCAGCAGGc                                                                                                                                           <  1:3358913/150‑1 (MQ=255)
           cGGCACGCCAATTTCTGCCGCCACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGc                                                                                                                                           <  1:1024015/150‑1 (MQ=255)
              cACGCCAATTTCTGCCGCCACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGt                                                                                                                                        >  1:1554249/1‑150 (MQ=255)
                cGCCAATTTCTGCCGCCACGGTTTCGACCCTGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTAt                                                                                                                                      >  2:3649013/1‑150 (MQ=255)
                cGCCAATTTCTGCCGCCACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTAt                                                                                                                                      <  1:4066121/150‑1 (MQ=255)
                 gCCAATTTCTGCCGCCACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATc                                                                                                                                     >  2:3441419/1‑150 (MQ=255)
                  ccAATTTCTGCCGCCACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCa                                                                                                                                    <  2:2015938/150‑1 (MQ=255)
                    aaTTTCTGCCGCCACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAg                                                                                                                                  <  1:3043513/150‑1 (MQ=255)
                        tCTGCCGCCACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGcaca                                                                                                                              >  1:32709/1‑150 (MQ=255)
                        tCTGCCGCCACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTAGCCCAGCAGGCGGTATCAAGcaca                                                                                                                              <  2:463273/150‑1 (MQ=255)
                          tGCCGCCACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAAt                                                                                                                            <  2:2692335/150‑1 (MQ=255)
                             cgccACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGc                                                                                                                         >  1:3548994/1‑150 (MQ=255)
                              gccACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCg                                                                                                                        <  1:2101161/150‑1 (MQ=255)
                              gccACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCg                                                                                                                        <  1:3140065/150‑1 (MQ=255)
                                cACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGAc                                                                                                                      >  2:777523/1‑150 (MQ=255)
                                   ggTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTc                                                                                                                   >  2:3537933/1‑150 (MQ=255)
                                     tttCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCg                                                                                                                 >  2:2544956/1‑150 (MQ=255)
                                         gACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGa                                                                                                             >  1:3085496/1‑150 (MQ=255)
                                         gACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGa                                                                                                             <  2:3780838/150‑1 (MQ=255)
                                         gACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACATGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGAGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGCCGa                                                                                                             >  1:3582162/1‑150 (MQ=255)
                                           cccAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGaca                                                                                                           >  2:1891265/1‑150 (MQ=255)
                                           cccAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGaca                                                                                                           >  1:757378/1‑150 (MQ=255)
                                            ccAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGacac                                                                                                          >  1:3947261/1‑150 (MQ=255)
                                             cAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACAcc                                                                                                         >  1:2594160/1‑150 (MQ=255)
                                               ggCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACcgc                                                                                                       <  1:3461367/150‑1 (MQ=255)
                                                gCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACcgcg                                                                                                      <  1:1656493/150‑1 (MQ=255)
                                                      aGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTg                                                                                                <  2:2500647/150‑1 (MQ=255)
                                                       gTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTgg                                                                                               >  1:4019865/1‑150 (MQ=255)
                                                          ttGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAg                                                                                            >  1:3380861/1‑150 (MQ=255)
                                                          ttGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAg                                                                                            >  1:1401408/1‑150 (MQ=255)
                                                            ggcggcGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGtt                                                                                          >  1:4194498/1‑150 (MQ=255)
                                                               ggcgCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGc                                                                                       >  1:4427675/1‑150 (MQ=255)
                                                               ggcgCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGc                                                                                       >  1:2517974/1‑150 (MQ=255)
                                                                  gcAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCccg                                                                                    >  2:3231041/1‑150 (MQ=255)
                                                                     aTCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCccgccg                                                                                 <  1:3188372/150‑1 (MQ=255)
                                                                     aTCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCccgccg                                                                                 >  2:3278552/1‑150 (MQ=255)
                                                                       cAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCcgcg                                                                               >  2:672025/1‑150 (MQ=255)
                                                                        aGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGt                                                                              <  2:891074/150‑1 (MQ=255)
                                                                        aGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGt                                                                              >  1:562296/1‑150 (MQ=255)
                                                                         gCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTa                                                                             <  2:3364973/150‑1 (MQ=255)
                                                                               tGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCa                                                                       <  1:2473956/150‑1 (MQ=255)
                                                                               tGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCa                                                                       <  1:956838/150‑1 (MQ=255)
                                                                                gTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCAc                                                                      <  2:3948430/150‑1 (MQ=255)
                                                                                  cGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACct                                                                    <  2:1556706/150‑1 (MQ=255)
                                                                                   gCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACctc                                                                   <  2:497117/150‑1 (MQ=255)
                                                                                      tAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTc                                                                >  1:3818708/1‑150 (MQ=255)
                                                                                      tAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTc                                                                >  2:154940/1‑150 (MQ=255)
                                                                                        acacCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGc                                                              >  1:1165455/1‑150 (MQ=255)
                                                                                         cacCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCt                                                             <  2:149857/150‑1 (MQ=255)
                                                                                         cacCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCt                                                             <  2:2464425/150‑1 (MQ=255)
                                                                                          acCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCAGAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTg                                                            >  2:4307630/1‑150 (MQ=255)
                                                                                             aGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGaa                                                         >  1:1714483/1‑150 (MQ=255)
                                                                                             aGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTAGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGaa                                                         <  1:2895799/150‑1 (MQ=255)
                                                                                             aGCATCGGCCCGGTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCCAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGaa                                                         <  2:3017975/150‑1 (MQ=255)
                                                                                               cATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACt                                                       <  2:803623/150‑1 (MQ=255)
                                                                                                aTCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACtt                                                      <  2:2789311/150‑1 (MQ=255)
                                                                                                  cGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGc                                                    >  1:4492224/1‑150 (MQ=255)
                                                                                                   ggCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGcc                                                   >  1:1278412/1‑150 (MQ=255)
                                                                                                   gacccGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTAGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGcc                                                   >  2:1206480/3‑150 (MQ=255)
                                                                                                    gCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGcca                                                  >  1:1998919/1‑150 (MQ=255)
                                                                                                     cccGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGccac                                                 <  2:4095463/150‑1 (MQ=255)
                                                                                                         ttACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGc                                             >  1:530495/1‑150 (MQ=255)
                                                                                                            ccAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCcac                                          <  1:4212454/150‑1 (MQ=255)
                                                                                                               aTCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACt                                       >  2:2158170/1‑150 (MQ=255)
                                                                                                                 cTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTAGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTc                                     >  2:4431939/1‑150 (MQ=255)
                                                                                                                  tGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTcc                                    >  2:1446944/1‑150 (MQ=255)
                                                                                                                  tGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTcc                                    >  2:2976070/1‑150 (MQ=255)
                                                                                                                      gATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACACTGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAg                                <  2:1183703/150‑1 (MQ=255)
                                                                                                                      gATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAg                                <  2:1065632/150‑1 (MQ=255)
                                                                                                                        tGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGt                              >  2:1227341/1‑150 (MQ=255)
                                                                                                                        tGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGt                              >  2:3520657/1‑150 (MQ=255)
                                                                                                                        tGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGt                              >  1:2761069/1‑150 (MQ=255)
                                                                                                                         ggTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTa                             <  1:904485/150‑1 (MQ=255)
                                                                                                                           ttAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGt                           <  1:4591407/150‑1 (MQ=255)
                                                                                                                             aGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTc                         <  2:3566008/150‑1 (MQ=255)
                                                                                                                             aGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTc                         <  1:413209/150‑1 (MQ=255)
                                                                                                                             aGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTc                         <  1:1448889/150‑1 (MQ=255)
                                                                                                                             aGCCGCGCCATAATCAAAGTTTTTAGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTc                         <  2:2212302/150‑1 (MQ=255)
                                                                                                                              gCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCg                        <  2:2605947/150‑1 (MQ=255)
                                                                                                                               ccGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCgg                       <  2:3151594/150‑1 (MQ=255)
                                                                                                                                 gcgcCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGcg                     <  1:1890679/150‑1 (MQ=255)
                                                                                                                                  cgcCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGcgt                    <  1:976333/150‑1 (MQ=255)
                                                                                                                                  cgcCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGcgt                    >  2:1815881/1‑150 (MQ=255)
                                                                                                                                   gcCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGcgtc                   <  2:3708511/150‑1 (MQ=255)
                                                                                                                                    ccATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGcgtcg                  >  2:2540957/1‑150 (MQ=255)
                                                                                                                                    ccATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGcgtcg                  <  2:2312775/150‑1 (MQ=255)
                                                                                                                                      aTAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTg                >  1:2644728/1‑150 (MQ=255)
                                                                                                                                       tAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGc               >  2:486414/1‑150 (MQ=255)
                                                                                                                                        aaTCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCa              >  1:1064620/1‑150 (MQ=255)
                                                                                                                                        aaTCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCa              >  2:1368379/1‑150 (MQ=255)
                                                                                                                                         aTCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAg             >  1:2827604/1‑150 (MQ=255)
                                                                                                                                           cAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGtt           >  1:4261939/1‑150 (MQ=255)
                                                                                                                                            aaaGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTc          <  1:3749805/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCTGCCTGCAACGGCACGCCAATTTCTGCCGCCACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCAGCGACTTGTCGCTTAACACCAGCATCGGCCCGTTACCAATCTGGCGATGGTTAGCCGCGCCATAATCAAAGTTTTTCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTA  >  CP009273/2498200‑2498497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑