Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,549,676 C→A 100% L28L (CTG→CTT)  eutL ← putative ethanol utilization carboxysome structural protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,549,6760CA97.5% 953.3 / 12.8 283L28L (CTG→CTT) eutLputative ethanol utilization carboxysome structural protein
Reads supporting (aligned to +/- strand):  ref base C (5/2);  new base A (131/145);  total (136/147)
Fisher's exact test for biased strand distribution p-value = 2.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 283 total aligned reads displayed.

CGGGCCGTGTGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTCAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGTTTCGCCAAAT  >  CP009273/2549529‑2549824
                                                                                                                                                   |                                                                                                                                                    
cGGGCCGTGTGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAg                                                                                                                                                    >  2:1706667/1‑150 (MQ=255)
cGGGCCGTGTGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAg                                                                                                                                                    >  2:263604/1‑150 (MQ=255)
  ggCCGTGTGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGtt                                                                                                                                                  >  1:3012027/1‑150 (MQ=255)
  ggCCGTGTGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGtt                                                                                                                                                  <  1:2836853/150‑1 (MQ=255)
   gCCGTGTGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTc                                                                                                                                                 <  1:2623580/150‑1 (MQ=255)
    ccGTGTGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCa                                                                                                                                                >  1:3151009/1‑150 (MQ=255)
    ccGTGTGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCa                                                                                                                                                <  2:2417269/150‑1 (MQ=255)
     cGTGTGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCAc                                                                                                                                               >  1:1708285/1‑150 (MQ=255)
     cGTGTGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCAc                                                                                                                                               <  2:4062949/150‑1 (MQ=255)
       tgtgCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCAcgc                                                                                                                                             >  1:3568117/1‑150 (MQ=255)
          gCAGCGCCCGCATAGAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCa                                                                                                                                          <  2:2545515/150‑1 (MQ=255)
               gcCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATc                                                                                                                                     <  1:3493145/150‑1 (MQ=255)
                  cGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGc                                                                                                                                  <  1:2567767/150‑1 (MQ=255)
                   gCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCg                                                                                                                                 >  1:2910270/1‑150 (MQ=255)
                    cATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGt                                                                                                                                >  1:4215489/1‑150 (MQ=255)
                    cATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGt                                                                                                                                >  1:663293/1‑150 (MQ=255)
                         agcgagcgGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACg                                                                                                                           <  1:2211033/150‑1 (MQ=255)
                         agcgagcgGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACg                                                                                                                           >  2:3904652/1‑150 (MQ=255)
                          gcgagcgGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACgg                                                                                                                          <  2:820342/150‑1 (MQ=255)
                             agcgGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAgg                                                                                                                       >  2:4074005/1‑150 (MQ=255)
                               cgGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCa                                                                                                                     <  1:3083820/150‑1 (MQ=255)
                                ggCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCaa                                                                                                                    >  2:2133540/1‑150 (MQ=255)
                                 gCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAAt                                                                                                                   >  2:4152700/1‑150 (MQ=255)
                                 gCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAAt                                                                                                                   >  2:2786755/1‑150 (MQ=255)
                                 gCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAAt                                                                                                                   >  1:1339397/1‑150 (MQ=255)
                                  ccATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCATAATCGGCGTTAACGGAGGCAATc                                                                                                                  <  2:4236668/150‑1 (MQ=255)
                                  ccATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATc                                                                                                                  <  2:3615730/150‑1 (MQ=255)
                                   cATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCa                                                                                                                 >  1:2010195/1‑150 (MQ=255)
                                   cATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCa                                                                                                                 >  1:1647374/1‑150 (MQ=255)
                                   cATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCa                                                                                                                 >  2:958109/1‑150 (MQ=255)
                                    aTACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCAc                                                                                                                >  1:4576666/1‑150 (MQ=255)
                                    aTACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCAc                                                                                                                <  1:1056508/150‑1 (MQ=255)
                                     tACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCAcc                                                                                                               <  1:1970389/150‑1 (MQ=255)
                                       caccacTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCg                                                                                                             <  2:628947/150‑1 (MQ=255)
                                       caccacTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCg                                                                                                             <  2:2159654/150‑1 (MQ=255)
                                       caccacTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCg                                                                                                             <  2:307743/150‑1 (MQ=255)
                                         ccacTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCGATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCa                                                                                                           >  1:1258898/1‑150 (MQ=255)
                                         ccacTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCa                                                                                                           >  1:2116189/1‑150 (MQ=255)
                                          cacTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCAt                                                                                                          <  1:2172398/150‑1 (MQ=255)
                                           acTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATa                                                                                                         >  1:101502/1‑150 (MQ=255)
                                            cTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAg                                                                                                        >  2:2257310/1‑150 (MQ=255)
                                                caccaTCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGt                                                                                                    <  2:285871/150‑1 (MQ=255)
                                                  ccaTCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGa                                                                                                  >  1:3830678/1‑150 (MQ=255)
                                                  ccaTCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGa                                                                                                  >  1:1398340/1‑150 (MQ=255)
                                                    ctCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACa                                                                                                <  2:1661973/149‑1 (MQ=255)
                                                       gCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGAc                                                                                             >  2:4338468/1‑150 (MQ=255)
                                                              tGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGaa                                                                                      >  2:4000829/1‑150 (MQ=255)
                                                               ggTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAAt                                                                                     >  2:3996502/1‑150 (MQ=255)
                                                               ggTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAAt                                                                                     <  1:285476/150‑1 (MQ=255)
                                                                   gCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCaaa                                                                                 >  2:2004903/1‑150 (MQ=255)
                                                                    cTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAAt                                                                                <  2:4113954/150‑1 (MQ=255)
                                                                         tcaGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTaaa                                                                           <  2:3058752/150‑1 (MQ=255)
                                                                          caGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAg                                                                          <  2:4125186/150‑1 (MQ=255)
                                                                           aGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGc                                                                         <  2:1295241/150‑1 (MQ=255)
                                                                           aGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGc                                                                         <  2:1816313/150‑1 (MQ=255)
                                                                            gCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCt                                                                        <  1:4637794/150‑1 (MQ=255)
                                                                                 aaTGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCat                                                                   <  2:2610982/150‑1 (MQ=255)
                                                                                 aaTGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCat                                                                   <  2:1943847/150‑1 (MQ=255)
                                                                                  aTGTAGGTGACGTCATCGGAGTCGGCAGAAATAAGACCGAGGCTACGAATATGCGGAGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAAACACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCatg                                                                  >  2:4019819/1‑150 (MQ=255)
                                                                                          gACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGcctcc                                                          <  2:2691111/150‑1 (MQ=255)
                                                                                           aCGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGcctcct                                                         >  1:2449517/1‑150 (MQ=255)
                                                                                            cGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCtt                                                        >  2:1474775/1‑150 (MQ=255)
                                                                                            cGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCtt                                                        <  1:1565227/150‑1 (MQ=255)
                                                                                            cGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCtt                                                        >  2:4578998/1‑150 (MQ=255)
                                                                                             gTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTa                                                       <  1:2053754/150‑1 (MQ=255)
                                                                                             gTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTa                                                       >  1:3533678/1‑150 (MQ=255)
                                                                                             gTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTa                                                       <  1:4328895/150‑1 (MQ=255)
                                                                                             gTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTa                                                       >  1:4502302/1‑150 (MQ=255)
                                                                                               cATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATc                                                     >  2:2966726/1‑150 (MQ=255)
                                                                                                  cGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCggg                                                  <  2:351297/150‑1 (MQ=255)
                                                                                                  cGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCggg                                                  <  2:37656/150‑1 (MQ=255)
                                                                                                   ggAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGt                                                 >  2:1014537/1‑150 (MQ=255)
                                                                                                    gAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGta                                                >  2:2807072/1‑149 (MQ=255)
                                                                                                    gAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTc                                                <  1:2477597/150‑1 (MQ=255)
                                                                                                     aGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTACAGCTGGCATGATGCCTCCTTATCGGGTCa                                               <  1:2691963/150‑1 (MQ=255)
                                                                                                      gTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCAt                                              <  2:418562/150‑1 (MQ=255)
                                                                                                        cGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGt                                            >  1:2707784/1‑150 (MQ=255)
                                                                                                         ggCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGtt                                           >  1:3310870/1‑150 (MQ=255)
                                                                                                                   aGACCGAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGAt                                 >  2:1154627/1‑150 (MQ=255)
                                                                                                                        gAGGCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCttt                            <  2:1306740/150‑1 (MQ=255)
                                                                                                                           gCTACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTg                         <  1:3286700/150‑1 (MQ=255)
                                                                                                                             tACGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCt                       >  2:4420717/1‑150 (MQ=255)
                                                                                                                              aCGAATATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTc                      <  1:2772436/150‑1 (MQ=255)
                                                                                                                               cGAATATGCGGCGGCAATTTCAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTcc                     <  1:816091/150‑1 (MQ=255)
                                                                                                                                 aaTATGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAg                   >  2:3205394/1‑150 (MQ=255)
                                                                                                                                  atatGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGc                  <  1:238909/150‑1 (MQ=255)
                                                                                                                                   tatGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCa                 >  2:3443971/1‑150 (MQ=255)
                                                                                                                                    atGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCAt                >  2:775910/1‑150 (MQ=255)
                                                                                                                                     tGCGGCGGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATa               <  2:4643957/150‑1 (MQ=255)
                                                                                                                                      gcggcgGCAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATTCTTTCTGCTCCAGCATAc              >  2:1033317/1‑150 (MQ=255)
                                                                                                                                        ggcggcAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGt            >  2:1334345/1‑150 (MQ=255)
                                                                                                                                        ggcggcAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGt            >  1:3703717/1‑150 (MQ=255)
                                                                                                                                        ggcggcAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGt            >  1:3703741/1‑150 (MQ=255)
                                                                                                                                        ggcggcAATTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGt            >  2:1334274/1‑150 (MQ=255)
                                                                                                                                              aaTTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGTTTCGcc      >  1:2947478/1‑150 (MQ=255)
                                                                                                                                              aaTTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGTTTCGcc      >  2:3848367/1‑150 (MQ=255)
                                                                                                                                              aaTTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTAATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGTTTCGcc      >  1:2258140/1‑150 (MQ=255)
                                                                                                                                               aTTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGTTTCGCCa     <  1:4003127/150‑1 (MQ=255)
                                                                                                                                               aTTTAAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGTTTCGCCa     <  2:3568117/150‑1 (MQ=255)
                                                                                                                                                  tCAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGTTTCGCCAAAt  <  2:1123520/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGGGCCGTGTGCAGCGCCCGCATATAGCGAGCGGCCATACACCACTTCCACCATCGCCTGCTTGGTCGCTTCATCAGCGGCAATGTAGGTGACGTCATCGGAGTCGGCAGAAATCAGACCGAGGCTACGAATATGCGGCGGCAATTTCAGTTCACGCGCAAAATCGGCGTTAACGGAGGCAATCACCCGCATAGCGGTGACAGACGGTCGAATCAAATCTAAAGCTGGCATGATGCCTCCTTATCGGGTCATGTTGATGCCGGATGCTTTCTGCTCCAGCATACGTTTCGCCAAAT  >  CP009273/2549529‑2549824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑