Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 201,962 A→T 100% D117V (GAT→GTT)  dnaE → DNA polymerase III alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273201,9620AT95.3% 883.2 / 26.6 274D117V (GAT→GTT) dnaEDNA polymerase III alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (7/6);  new base T (141/120);  total (148/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.43e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 285 total aligned reads displayed.

CAGATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCGCTAT  >  CP009273/201815‑202110
                                                                                                                                                   |                                                                                                                                                    
cAGATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCatcgttc                                                                                                                                                    <  1:563229/150‑1 (MQ=255)
cAGATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCatcgttc                                                                                                                                                    <  1:1876737/150‑1 (MQ=255)
 aGATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCatcgttcg                                                                                                                                                   <  2:4405476/150‑1 (MQ=255)
  gATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTcgc                                                                                                                                                  >  1:1225936/1‑150 (MQ=255)
  gATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTcgc                                                                                                                                                  >  2:2181073/1‑150 (MQ=255)
  gATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATcgc                                                                                                                                                  <  1:1268641/150‑1 (MQ=255)
    ttttAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGa                                                                                                                                                >  2:2672234/1‑150 (MQ=255)
       tAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTg                                                                                                                                             >  2:2586150/1‑150 (MQ=255)
       tAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTg                                                                                                                                             <  2:1065509/150‑1 (MQ=255)
          cGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCt                                                                                                                                          <  2:4396170/150‑1 (MQ=255)
           gTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCtt                                                                                                                                         >  2:2868742/1‑150 (MQ=255)
           gTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCtt                                                                                                                                         <  1:2558005/150‑1 (MQ=255)
             ccAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTAt                                                                                                                                       <  2:3568995/150‑1 (MQ=255)
             ccAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTAt                                                                                                                                       <  2:3920365/150‑1 (MQ=255)
             ccAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTAt                                                                                                                                       <  2:1751659/150‑1 (MQ=255)
             ccAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTAt                                                                                                                                       >  1:2462857/1‑150 (MQ=255)
                  gCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACAGGCTTATCGAAt                                                                                                                                  <  2:4075994/150‑1 (MQ=255)
                  gCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTATCGAAt                                                                                                                                  <  1:964575/150‑1 (MQ=255)
                   cGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAAtt                                                                                                                                 >  2:868833/1‑150 (MQ=255)
                    gACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTa                                                                                                                                >  2:3201711/1‑150 (MQ=255)
                     aCCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTaa                                                                                                                               <  1:3713429/150‑1 (MQ=255)
                       ctgctgGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTaaat                                                                                                                             <  1:4406884/150‑2 (MQ=255)
                       ctgctgGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAAc                                                                                                                             <  2:3295406/150‑1 (MQ=255)
                          ctgGCTGATGAGTAAACCCACCTGACGCAACTTGCGGCGAACAATACCTTCTATCAGAAACTGACTTTGCAGATATCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCAACGTACGCGACTGGCTGATCGAATAAAACaaa                                                                                                                          >  2:1154487/1‑150 (MQ=255)
                            gggTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAgg                                                                                                                        <  1:1523666/150‑1 (MQ=255)
                             ggTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAggg                                                                                                                       >  1:682718/1‑150 (MQ=255)
                              gTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGt                                                                                                                      <  2:1382629/150‑1 (MQ=255)
                               tgatgaGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGtt                                                                                                                     >  1:821236/1‑150 (MQ=255)
                                gatgaGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTg                                                                                                                    <  2:849980/150‑1 (MQ=255)
                                gatgaGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTg                                                                                                                    >  1:1916974/1‑150 (MQ=255)
                                 atgaGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGa                                                                                                                   <  1:2719909/150‑1 (MQ=255)
                                    aGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATcc                                                                                                                <  2:2945228/150‑1 (MQ=255)
                                    aGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATcc                                                                                                                <  1:4617993/150‑1 (MQ=255)
                                    aGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATcc                                                                                                                >  2:1183525/1‑150 (MQ=255)
                                     gTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCct                                                                                                               <  2:3667230/150‑1 (MQ=255)
                                        aaCCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCcttct                                                                                                            >  1:2138444/1‑150 (MQ=255)
                                           ccACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTc                                                                                                         <  2:565354/150‑1 (MQ=255)
                                            cACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTcc                                                                                                        <  2:1935316/150‑1 (MQ=255)
                                             aCCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCcg                                                                                                       >  2:1458104/1‑150 (MQ=255)
                                                   cGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGAc                                                                                                 >  1:3055621/1‑150 (MQ=255)
                                                           gcggcgAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGc                                                                                         <  2:2088444/150‑1 (MQ=255)
                                                            cggcgAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCg                                                                                        >  1:1351527/1‑150 (MQ=255)
                                                             ggcgAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGa                                                                                       >  2:2307431/1‑150 (MQ=255)
                                                             ggcgAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGa                                                                                       >  2:1843451/1‑150 (MQ=255)
                                                             ggcgAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGa                                                                                       >  2:833944/1‑150 (MQ=255)
                                                               cgAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACg                                                                                     >  2:4261090/1‑150 (MQ=255)
                                                                gAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGt                                                                                    <  1:3386153/150‑1 (MQ=255)
                                                                 aaCAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTc                                                                                   <  2:3859179/150‑1 (MQ=255)
                                                                 aaCAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTc                                                                                   <  1:3264570/150‑1 (MQ=255)
                                                                   cAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCgg                                                                                 >  2:3770069/1‑150 (MQ=255)
                                                                    aaTACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGa                                                                                >  1:601944/1‑150 (MQ=255)
                                                                     aTACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGAc                                                                               >  1:1005955/1‑150 (MQ=255)
                                                                      tACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACg                                                                              <  1:4383319/150‑1 (MQ=255)
                                                                        ccGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCa                                                                            >  2:452000/1‑150 (MQ=255)
                                                                         cGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAg                                                                           >  1:3471015/1‑150 (MQ=255)
                                                                               tCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCtttt                                                                     >  1:4216964/1‑150 (MQ=255)
                                                                                cAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTg                                                                    >  1:2631759/1‑150 (MQ=255)
                                                                                cAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTg                                                                    <  1:2181073/150‑1 (MQ=255)
                                                                                 aGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGc                                                                   >  2:1867496/1‑150 (MQ=255)
                                                                                    aTCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTg                                                                <  2:3652268/150‑1 (MQ=255)
                                                                                     tCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTgg                                                               <  1:3541052/150‑1 (MQ=255)
                                                                                     tCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTgg                                                               <  1:161652/150‑1 (MQ=255)
                                                                                      cTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGt                                                              >  2:4226660/1‑150 (MQ=255)
                                                                                       tGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTa                                                             >  2:3840121/1‑150 (MQ=255)
                                                                                         aCGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAAc                                                           >  2:4594870/1‑150 (MQ=255)
                                                                                          cGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACa                                                          <  1:2901525/150‑1 (MQ=255)
                                                                                               cTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAgcgcg                                                     >  2:620091/1‑150 (MQ=255)
                                                                                                   tctcAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTgg                                                 >  1:3599736/1‑150 (MQ=255)
                                                                                                    ctcAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGt                                                >  1:3913124/1‑150 (MQ=255)
                                                                                                    ctcAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCGTTTGCGTGGTAACAGCGCGCTGGt                                                >  1:3858161/1‑150 (MQ=255)
                                                                                                     tcAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTa                                               <  2:2627309/150‑1 (MQ=255)
                                                                                                       aaaaGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGa                                             >  2:2451464/1‑150 (MQ=255)
                                                                                                        aaaGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAATAGCGCGCTGGTAGAt                                            >  1:2936677/1‑150 (MQ=255)
                                                                                                         aaGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATg                                           >  1:1551697/1‑150 (MQ=255)
                                                                                                         aaGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATg                                           >  1:4006119/1‑150 (MQ=255)
                                                                                                          aGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGATTTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGa                                          <  1:2248610/150‑1 (MQ=255)
                                                                                                          aGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGa                                          >  1:3675489/1‑150 (MQ=255)
                                                                                                             gTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAgtg                                       >  1:1722452/1‑150 (MQ=255)
                                                                                                               aTCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAgtgtg                                     <  1:4091049/150‑1 (MQ=255)
                                                                                                                  aGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTcg                                  <  2:4216907/150‑1 (MQ=255)
                                                                                                                  aGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTcg                                  >  2:733842/1‑150 (MQ=255)
                                                                                                                    cgcgGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTcgcg                                >  2:2050539/1‑150 (MQ=255)
                                                                                                                     gcgGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGt                               >  2:551535/1‑150 (MQ=255)
                                                                                                                       gggTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGttt                             <  2:2972011/150‑1 (MQ=255)
                                                                                                                          tACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTAt                          >  1:4504659/1‑150 (MQ=255)
                                                                                                                             ggTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATgaa                       <  1:1239908/150‑1 (MQ=255)
                                                                                                                             ggTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATgaa                       >  2:3325636/1‑150 (MQ=255)
                                                                                                                               tGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATgaaga                     >  2:3614867/1‑150 (MQ=255)
                                                                                                                                  cgccgGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAaca                  <  2:475201/150‑1 (MQ=255)
                                                                                                                                  cgccgGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAaca                  >  1:2031081/1‑150 (MQ=255)
                                                                                                                                  cgccgGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAaca                  <  2:3502886/150‑1 (MQ=255)
                                                                                                                                    ccgGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACt                <  1:1848319/150‑1 (MQ=255)
                                                                                                                                    ccgGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACt                <  1:863651/150‑1 (MQ=255)
                                                                                                                                       ggCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACTAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGAGGAGTGTGTCGCGTTTTATGAAGAACACTTcc             >  1:1155198/1‑150 (MQ=255)
                                                                                                                                       ggCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTcc             <  1:3802537/150‑1 (MQ=255)
                                                                                                                                        gCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTccc            >  1:333974/1‑150 (MQ=255)
                                                                                                                                          cGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGTGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCgg          >  1:4041769/1‑150 (MQ=255)
                                                                                                                                             tcatcGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATc       >  2:2478006/1‑150 (MQ=255)
                                                                                                                                             tcatcGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATc       >  1:2412107/1‑150 (MQ=255)
                                                                                                                                                tcgttcgCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCGCt    <  2:3016618/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CAGATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCGCTAT  >  CP009273/201815‑202110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑