Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,733,753 C→T 100% E253K (GAG→AAG)  aroF ← 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,733,7530CT97.7% 887.6 / 7.1 255E253K (GAG→AAG) aroF3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible
Reads supporting (aligned to +/- strand):  ref base C (3/3);  new base T (136/113);  total (139/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 260 total aligned reads displayed.

TCATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCG  >  CP009273/2733606‑2733898
                                                                                                                                                   |                                                                                                                                                 
  atcaGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCtttttt                                                                                                                                               <  1:3384516/150‑1 (MQ=255)
   tcaGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTtttttt                                                                                                                                              >  1:4215286/1‑150 (MQ=255)
     aGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTca                                                                                                                                            >  1:3266750/1‑150 (MQ=255)
       aCCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTcaca                                                                                                                                          >  1:1041646/1‑150 (MQ=255)
        ccAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACAt                                                                                                                                         >  2:2152004/1‑150 (MQ=255)
         cAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACAtt                                                                                                                                        <  2:3871397/150‑1 (MQ=255)
         cAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACAtt                                                                                                                                        >  1:2309396/1‑150 (MQ=255)
          aataatTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTg                                                                                                                                       <  1:88779/150‑1 (MQ=255)
           ataatTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGGTCCATCTTTTTTTCACATTgc                                                                                                                                      >  1:3992884/1‑150 (MQ=255)
            taatTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTgcg                                                                                                                                     <  1:346333/150‑1 (MQ=255)
             aatTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTgcgc                                                                                                                                    >  1:2860930/1‑150 (MQ=255)
              atTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCa                                                                                                                                   >  2:3573798/1‑150 (MQ=255)
               ttGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCaa                                                                                                                                  >  2:262991/1‑150 (MQ=255)
               ttGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCaa                                                                                                                                  <  2:3656119/150‑1 (MQ=255)
               ttGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACAATGCGCaa                                                                                                                                  >  2:449440/1‑150 (MQ=255)
                     gATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCg                                                                                                                            >  2:219243/1‑150 (MQ=255)
                       ttGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCa                                                                                                                          >  1:776387/1‑150 (MQ=255)
                       ttGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCa                                                                                                                          >  1:4462342/1‑150 (MQ=255)
                         gCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAgg                                                                                                                        <  1:3343665/150‑1 (MQ=255)
                          ccATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAggg                                                                                                                       >  2:2521659/1‑150 (MQ=255)
                          ccATCTTTGATTTGAGCAACCACGGATTATGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTATTTCACATTGCGCAACATCCGCAggg                                                                                                                       <  1:328389/150‑1 (MQ=255)
                            aTCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCt                                                                                                                     >  2:2071713/1‑150 (MQ=255)
                             tCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCta                                                                                                                    >  1:4378417/1‑150 (MQ=255)
                             tCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCta                                                                                                                    <  2:3574453/150‑1 (MQ=255)
                             tCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCAATCTTTTTTTCACATTGCGCAACATCCGCAGGGCta                                                                                                                    <  2:4048657/150‑1 (MQ=255)
                              cTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCtat                                                                                                                   <  1:1586074/150‑1 (MQ=255)
                              cTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCtat                                                                                                                   >  1:1125159/1‑150 (MQ=255)
                               tttgatttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCtata                                                                                                                  <  1:3423391/150‑1 (MQ=255)
                               tttgatttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCtata                                                                                                                  <  1:2996908/150‑1 (MQ=255)
                                ttgatttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAg                                                                                                                 <  1:163089/150‑1 (MQ=255)
                                 tgatttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGt                                                                                                                <  2:872099/150‑1 (MQ=255)
                                  gatttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGtt                                                                                                               >  2:2893714/1‑150 (MQ=255)
                                         gCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCt                                                                                                        >  1:1767615/1‑150 (MQ=255)
                                         gCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCt                                                                                                        >  1:3956105/1‑150 (MQ=255)
                                         gCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCt                                                                                                        <  1:2490606/150‑1 (MQ=255)
                                           aaCCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCttt                                                                                                      <  2:2725069/150‑1 (MQ=255)
                                           aaCCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCttt                                                                                                      <  2:2100367/150‑1 (MQ=255)
                                           aaCCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCttt                                                                                                      <  2:4468371/150‑1 (MQ=255)
                                               aCGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTacca                                                                                                  >  2:3201628/1‑150 (MQ=255)
                                                 ggATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTaccacc                                                                                                >  1:1388198/1‑150 (MQ=255)
                                                 ggATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTaccacc                                                                                                >  1:1388100/1‑150 (MQ=255)
                                                  gATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACcg                                                                                               >  1:271927/1‑150 (MQ=255)
                                                  gATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGGTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACcg                                                                                               >  1:3542369/1‑150 (MQ=255)
                                                    ttCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACcgcg                                                                                             >  2:2474799/1‑150 (MQ=255)
                                                    ttCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACcgcg                                                                                             >  2:742019/1‑150 (MQ=255)
                                                       tGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAg                                                                                          <  1:1033898/150‑1 (MQ=255)
                                                       tGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAg                                                                                          >  1:2310999/1‑150 (MQ=255)
                                                       tGCCACCGCAGGCTGACGGCGATAATCTTTACTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAg                                                                                          <  1:2713422/150‑1 (MQ=255)
                                                          cACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGAt                                                                                       <  2:1559495/150‑1 (MQ=255)
                                                           aCCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATc                                                                                      <  1:2797557/150‑1 (MQ=255)
                                                              gCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATcaca                                                                                   <  1:1291409/150‑1 (MQ=255)
                                                              gCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATcaca                                                                                   <  1:3614817/150‑1 (MQ=255)
                                                                   cTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCACCATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGcc                                                                              <  2:1025/150‑1 (MQ=255)
                                                                   cTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGcc                                                                              <  1:817433/150‑1 (MQ=255)
                                                                   cTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGcc                                                                              <  1:1517362/150‑1 (MQ=255)
                                                                   cTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGcc                                                                              <  1:3256711/150‑1 (MQ=255)
                                                                    tGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCg                                                                             >  1:4456605/1‑150 (MQ=255)
                                                                    tGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCg                                                                             >  2:2864471/1‑150 (MQ=255)
                                                                    tGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCg                                                                             <  2:234817/150‑1 (MQ=255)
                                                                       cGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTcc                                                                          >  2:1862925/1‑150 (MQ=255)
                                                                         gCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCgg                                                                        <  2:2880111/150‑1 (MQ=255)
                                                                          cGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGa                                                                       >  2:628427/1‑150 (MQ=255)
                                                                          cGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGa                                                                       >  2:3771948/1‑150 (MQ=255)
                                                                           gATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGAt                                                                      >  2:2098696/1‑150 (MQ=255)
                                                                           gATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGAt                                                                      >  1:3744567/1‑150 (MQ=255)
                                                                           gATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGAt                                                                      <  2:49754/150‑1 (MQ=255)
                                                                           gATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGAt                                                                      >  2:1203399/1‑150 (MQ=255)
                                                                            aTAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGAtt                                                                     >  1:947343/1‑150 (MQ=255)
                                                                              aaTCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTcc                                                                   >  2:1911398/1‑150 (MQ=255)
                                                                                  tttATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTg                                                               <  2:3804427/150‑1 (MQ=255)
                                                                                    tATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAg                                                             <  1:746137/150‑1 (MQ=255)
                                                                                      ttGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGtt                                                           >  2:4268019/1‑150 (MQ=255)
                                                                                          aaTTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTa                                                       <  1:3078438/150‑1 (MQ=255)
                                                                                            ttACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAgc                                                     >  1:2748665/1‑150 (MQ=255)
                                                                                              aCCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAgcaa                                                   >  1:1533662/1‑150 (MQ=255)
                                                                                              aCCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAgcaa                                                   >  2:3066694/1‑150 (MQ=255)
                                                                                                 gTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAgcaacgc                                                >  1:3768778/1‑150 (MQ=255)
                                                                                                 gTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAgcaacgc                                                >  2:1694120/1‑150 (MQ=255)
                                                                                                  tGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAgcaacgca                                               <  2:389420/150‑1 (MQ=255)
                                                                                                      tGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcct                                           >  2:133303/1‑150 (MQ=255)
                                                                                                      tGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcct                                           >  1:1111051/1‑150 (MQ=255)
                                                                                                              aCCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACctgccctgcc                                   <  1:3573798/150‑1 (MQ=255)
                                                                                                                cATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCcctgcctg                                 <  2:1930808/150‑1 (MQ=255)
                                                                                                                   cAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGtt                              >  1:4155124/1‑150 (MQ=255)
                                                                                                                   cAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGtt                              >  1:4155158/1‑150 (MQ=255)
                                                                                                                    agagACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTa                             >  1:2042464/1‑150 (MQ=255)
                                                                                                                       gaCGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATg                          >  2:738372/1‑150 (MQ=255)
                                                                                                                         cGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGcc                        >  2:4608214/1‑150 (MQ=255)
                                                                                                                           ggCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCcaa                      <  2:2656795/150‑1 (MQ=255)
                                                                                                                             agcAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCcaaca                    <  2:1750897/149‑1 (MQ=255)
                                                                                                                              gcAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCcaacaa                   >  1:333919/1‑150 (MQ=255)
                                                                                                                               cAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACaaa                  >  1:4241923/1‑150 (MQ=255)
                                                                                                                                   cccGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACgg              <  1:2445910/150‑1 (MQ=255)
                                                                                                                                      gCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGc           >  2:1397076/1‑150 (MQ=255)
                                                                                                                                         tGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGc        <  2:3351186/150‑1 (MQ=255)
                                                                                                                                           ttCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTg      >  1:1862322/1‑150 (MQ=255)
                                                                                                                                            tCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTACGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTgg     >  1:2232272/1‑150 (MQ=255)
                                                                                                                                               aTCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGggcg  <  2:4303906/150‑1 (MQ=255)
                                                                                                                                               aTCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGggcg  >  2:3434613/1‑150 (MQ=255)
                                                                                                                                               aTCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTGTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGggcg  <  1:404885/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TCATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCG  >  CP009273/2733606‑2733898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑