Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,778,281 C→T 12.9% pseudogene (90/1374 nt) ypjC ← pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,778,2810CT12.9% 800.1 / 80.5 263pseudogene (90/1374 nt)ypjCpseudogene
Reads supporting (aligned to +/- strand):  ref base C (123/105);  new base T (15/19);  total (138/125)
Fisher's exact test for biased strand distribution p-value = 3.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 266 total aligned reads displayed.

ATTGTTCTCCAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACTA  >  CP009273/2778133‑2778430
                                                                                                                                                    |                                                                                                                                                     
aTTGTTCTCCAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCt                                                                                                                                                      >  1:906788/1‑150 (MQ=255)
  tGTTCTCCAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTcc                                                                                                                                                    >  1:2892768/1‑150 (MQ=255)
  tGTTCTCCAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTAAAAATACTATGAGCTAGTAGCTcc                                                                                                                                                    <  1:2647400/150‑1 (MQ=255)
    ttCTCCAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGTTCCAt                                                                                                                                                  <  1:1939121/150‑1 (MQ=255)
     tctcCAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGTTCCAtg                                                                                                                                                 >  2:1611036/1‑150 (MQ=255)
     tctcCAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTAAAAATACTATGAGCTAGTAGCTCCAtg                                                                                                                                                 >  2:93303/1‑150 (MQ=255)
        ccAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGTTCCATgtgt                                                                                                                                              <  1:3834060/150‑1 (MQ=255)
         cAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTAAAAATACTATGAGCTAGTAGCTCCATGTGtt                                                                                                                                             <  2:2350605/150‑1 (MQ=255)
         cAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTAAAAATACTATGAGCTAGTAGCTCCATGTGtt                                                                                                                                             <  2:1369268/150‑1 (MQ=255)
          aGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGTTCCATGTGttt                                                                                                                                            <  2:2031239/150‑1 (MQ=255)
          aGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGttt                                                                                                                                            <  2:869204/150‑1 (MQ=255)
          aGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGttt                                                                                                                                            >  2:1760239/1‑150 (MQ=255)
          aGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTAAAAATACTATGAGCTAGTAGCTCCATGTGttt                                                                                                                                            >  2:4510768/1‑150 (MQ=255)
           gCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTa                                                                                                                                           <  2:1070249/150‑1 (MQ=255)
           gCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTa                                                                                                                                           <  2:3639833/150‑1 (MQ=255)
            cTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTag                                                                                                                                          <  1:1989081/150‑1 (MQ=255)
            cTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTAAAAATACTATGAGCTAGTAGCTCCATGTGTTTag                                                                                                                                          <  1:1023781/150‑1 (MQ=255)
            cTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTAAAAATACTATGAGCTAGTAGCTCCATGTGTTTag                                                                                                                                          >  1:1751000/1‑150 (MQ=255)
             tCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTaga                                                                                                                                         >  1:4361713/1‑150 (MQ=255)
             tCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTaga                                                                                                                                         >  1:854324/1‑150 (MQ=255)
              cccATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTagaa                                                                                                                                        <  2:2006807/150‑1 (MQ=255)
                  tAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGatt                                                                                                                                    <  2:460027/150‑1 (MQ=255)
                   aaTGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGattt                                                                                                                                   <  2:2666172/150‑1 (MQ=255)
                   aaTGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGattt                                                                                                                                   <  2:3977287/150‑1 (MQ=255)
                    aTGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGattta                                                                                                                                  <  1:2224578/150‑1 (MQ=255)
                     tGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGatttat                                                                                                                                 <  1:4161836/150‑1 (MQ=255)
                      gACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTAAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGatttatt                                                                                                                                <  1:26887/150‑1 (MQ=255)
                         aaaGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGTTCCATGTGTTTAGAAGATTTATTTTc                                                                                                                             >  2:755862/1‑150 (MQ=255)
                         aaaGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTc                                                                                                                             >  2:3818108/1‑150 (MQ=255)
                         aaaGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTAAAAATATTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTc                                                                                                                             >  2:3586782/1‑150 (MQ=255)
                         aaaGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTAAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTc                                                                                                                             >  1:1787325/1‑150 (MQ=255)
                         aaaGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTAAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTc                                                                                                                             >  1:568279/1‑150 (MQ=255)
                          aaGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTAAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCa                                                                                                                            >  2:900407/1‑150 (MQ=255)
                            ggCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGTTCCATGTGTTTAGAAGATTTATTTTCAtt                                                                                                                          >  2:1956197/1‑150 (MQ=255)
                              cGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATtat                                                                                                                        <  2:1505100/150‑1 (MQ=255)
                              cGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTAAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATtat                                                                                                                        >  1:2977355/1‑150 (MQ=255)
                               gTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATtata                                                                                                                       >  1:3546174/1‑150 (MQ=255)
                               gTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATtata                                                                                                                       >  1:1223421/1‑150 (MQ=255)
                                     ttcttGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTAAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTcc                                                                                                                 <  2:1130701/150‑1 (MQ=255)
                                          gATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTAc                                                                                                            <  2:374325/150‑1 (MQ=255)
                                                taCATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCaaaa                                                                                                      >  1:406007/1‑150 (MQ=255)
                                                 aCATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAAc                                                                                                     >  1:1609499/1‑150 (MQ=255)
                                                  cATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACt                                                                                                    <  2:155773/150‑1 (MQ=255)
                                                  cATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACt                                                                                                    <  2:3243285/150‑1 (MQ=255)
                                                    ttCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGTTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTgc                                                                                                  <  1:4125229/150‑1 (MQ=255)
                                                    ttCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTAAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTgc                                                                                                  >  1:4133610/1‑150 (MQ=255)
                                                      cAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTgcgc                                                                                                <  2:1685591/150‑1 (MQ=255)
                                                      cAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTgcgc                                                                                                >  1:1077189/1‑150 (MQ=255)
                                                       aGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGTTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCt                                                                                               >  1:3921713/1‑150 (MQ=255)
                                                       aGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTAAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCt                                                                                               >  2:541343/1‑150 (MQ=255)
                                                         aCATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAg                                                                                             <  2:3300593/150‑1 (MQ=255)
                                                          catcatTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGc                                                                                            >  1:3881340/1‑150 (MQ=255)
                                                              atTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTa                                                                                        <  2:1997300/150‑1 (MQ=255)
                                                                   ttGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATcc                                                                                   <  1:4477086/150‑1 (MQ=255)
                                                                   ttGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTAAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATcc                                                                                   <  1:1447413/150‑1 (MQ=255)
                                                                     gTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGt                                                                                 >  1:1652641/1‑150 (MQ=255)
                                                                     gTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGt                                                                                 >  2:3523041/1‑150 (MQ=255)
                                                                        aaGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGTTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGc                                                                              <  1:2145520/150‑1 (MQ=255)
                                                                        aaGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGc                                                                              >  1:2078033/1‑150 (MQ=255)
                                                                         aGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCt                                                                             >  2:3406163/1‑150 (MQ=255)
                                                                          gTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGTTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCtt                                                                            <  2:4465668/150‑1 (MQ=255)
                                                                          gTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCtt                                                                            <  2:1212420/150‑1 (MQ=255)
                                                                            ttCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCtttt                                                                          >  2:113775/1‑150 (MQ=255)
                                                                               aaGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGTTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTact                                                                       >  2:3922907/1‑150 (MQ=255)
                                                                                agagCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTAAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTacta                                                                      >  2:3955290/1‑150 (MQ=255)
                                                                                 gagCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTactaa                                                                     <  1:777134/150‑1 (MQ=255)
                                                                                 gagCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTactaa                                                                     <  1:2313183/150‑1 (MQ=255)
                                                                                  agCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAGGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTactaac                                                                    >  1:1154491/1‑150 (MQ=255)
                                                                                    cACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTAAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTactaacta                                                                  >  2:46251/1‑150 (MQ=255)
                                                                                         cgATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTAAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACAtt                                                             <  1:3822220/150‑1 (MQ=255)
                                                                                               gTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTAt                                                       >  1:2298829/1‑150 (MQ=255)
                                                                                                   ctGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCt                                                   <  1:3319601/150‑1 (MQ=255)
                                                                                                    tGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTc                                                  >  2:3134932/1‑150 (MQ=255)
                                                                                                     gATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGTTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCa                                                 <  1:3662219/150‑1 (MQ=255)
                                                                                                       tGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGTTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAAt                                               <  1:4574584/150‑1 (MQ=255)
                                                                                                       tGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAAt                                               <  2:906788/150‑1 (MQ=255)
                                                                                                        gCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAAta                                              <  2:1381882/150‑2 (MQ=255)
                                                                                                          aGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGTTCCATGTGTTTAGAAGATTTATTTTCATTATAATTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTaa                                            >  1:3521759/1‑150 (MQ=255)
                                                                                                          aGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTaa                                            >  2:615823/1‑150 (MQ=255)
                                                                                                          aGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTaa                                            >  2:1946982/1‑150 (MQ=255)
                                                                                                           gCTTCCTTACAAATTAATTTAAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAAt                                           >  2:2988173/1‑150 (MQ=255)
                                                                                                            cTTCCTTACAAATTAATTTAAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATg                                          >  2:530085/1‑150 (MQ=255)
                                                                                                             ttCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGt                                         >  1:1294234/1‑150 (MQ=255)
                                                                                                               ccTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCt                                       <  1:2244825/150‑1 (MQ=255)
                                                                                                                  tACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACa                                    >  1:2512395/1‑150 (MQ=255)
                                                                                                                  tACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACa                                    >  2:4303003/1‑150 (MQ=255)
                                                                                                                     aaaTTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATgg                                 <  2:2531341/150‑1 (MQ=255)
                                                                                                                      aattaattTAAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGc                                <  2:3875512/150‑1 (MQ=255)
                                                                                                                            ttttAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTAtttt                          >  1:1501661/1‑150 (MQ=255)
                                                                                                                            tttaAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTAtttt                          >  2:2282232/1‑150 (MQ=255)
                                                                                                                                aaaaTACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAAt                      <  2:1462488/150‑1 (MQ=255)
                                                                                                                                    tACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGtta                  >  2:1133200/1‑150 (MQ=255)
                                                                                                                                     aCTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGttat                 >  1:3079567/1‑150 (MQ=255)
                                                                                                                                      cTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGttatt                <  2:4171729/150‑1 (MQ=255)
                                                                                                                                         tGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACt             <  1:30275/150‑1 (MQ=255)
                                                                                                                                              tagtagCTCCATGTGTTTAGAATATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTAATAACTAACATTGTCTATTCCTCAATTAATTTCTACATGGCTATTTTTAATGTTATTAATGTTTg        >  2:2383578/1‑150 (MQ=255)
                                                                                                                                              tagtagCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTg        >  2:278232/1‑150 (MQ=255)
                                                                                                                                                   gCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACt   <  1:4220156/150‑1 (MQ=255)
                                                                                                                                                    cTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACta  <  2:1814194/150‑1 (MQ=255)
                                                                                                                                                    cTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACta  <  2:1252607/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATTGTTCTCCAGCTCCCATAATGACAAAGGCGTTGCCTTCTTGATATATACATTCAGACATCATTTTTTGTAAAGTTTCAAGAGCACCGCGATACGTCTCTGATGCAGCTTCCTTACAAATTAATTTTAAAATACTATGAGCTAGTAGCTCCATGTGTTTAGAAGATTTATTTTCATTATAACTTCCACTACCCAAAACTGCGCTAGCGTTAAATCCGTTGCTTTTACTAACTAACATTGTCTATTCCTCAATTAATGTCTACATGGCTATTTTTAATGTTATTACTGTTTGTCACTA  >  CP009273/2778133‑2778430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑