Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 232,860 G→T 100% D103Y (GAT→TAT)  dnaQ → DNA polymerase III epsilon subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273232,8600GT97.2% 1066.2 / 18.4 317D103Y (GAT→TAT) dnaQDNA polymerase III epsilon subunit
Reads supporting (aligned to +/- strand):  ref base G (5/4);  new base T (140/168);  total (145/172)
Fisher's exact test for biased strand distribution p-value = 7.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 317 total aligned reads displayed.

CCCGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTC  >  CP009273/232713‑233004
                                                                                                                                                   |                                                                                                                                                
cccGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGTTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTCTATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTat                                                                                                                                                <  1:2316230/150‑1 (MQ=255)
  cGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTatat                                                                                                                                              <  1:2385158/150‑1 (MQ=255)
   gATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATc                                                                                                                                             >  2:1192412/1‑150 (MQ=255)
      cGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGc                                                                                                                                          >  1:335595/1‑150 (MQ=255)
         cTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCttt                                                                                                                                       <  2:3171915/150‑1 (MQ=255)
          tggtggATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTa                                                                                                                                      >  2:4631229/1‑150 (MQ=255)
             tggATCTGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATgg                                                                                                                                   >  2:2708508/1‑150 (MQ=255)
              ggATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGa                                                                                                                                  >  1:3595349/1‑150 (MQ=255)
              ggATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGa                                                                                                                                  <  1:340530/150‑1 (MQ=255)
              ggATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGa                                                                                                                                  >  1:2664006/1‑150 (MQ=255)
              ggATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGa                                                                                                                                  >  1:2638114/1‑150 (MQ=255)
               gATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGAc                                                                                                                                 >  2:2036877/1‑150 (MQ=255)
                     gAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAg                                                                                                                           >  1:4118417/1‑150 (MQ=255)
                       aGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGtt                                                                                                                         <  1:3768319/150‑1 (MQ=255)
                        gCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGttt                                                                                                                        >  2:576076/1‑150 (MQ=255)
                         ccTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCTTGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGtttt                                                                                                                       <  1:501200/150‑1 (MQ=255)
                         ccTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGtttt                                                                                                                       <  1:1555813/150‑1 (MQ=255)
                          cTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTc                                                                                                                      >  1:2174276/1‑150 (MQ=255)
                                 gTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCtt                                                                                                               <  2:3818580/150‑1 (MQ=255)
                                 gTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCtt                                                                                                               <  2:1914276/150‑1 (MQ=255)
                                  tACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTa                                                                                                              <  1:767455/150‑1 (MQ=255)
                                   aCATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTaa                                                                                                             >  1:849006/1‑150 (MQ=255)
                                    cATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAg                                                                                                            <  1:3075827/150‑1 (MQ=255)
                                     aTGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAgc                                                                                                           >  2:1274038/1‑150 (MQ=255)
                                          aTTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGat                                                                                                      >  2:1320056/1‑150 (MQ=255)
                                             gCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATAtt                                                                                                   <  1:3959101/150‑1 (MQ=255)
                                               cGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATACTcc                                                                                                 >  1:1022401/1‑150 (MQ=255)
                                                gATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCg                                                                                                <  2:2215962/150‑1 (MQ=255)
                                                gATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCg                                                                                                <  2:1008966/150‑1 (MQ=255)
                                                gATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCg                                                                                                <  1:3612229/150‑1 (MQ=255)
                                                 aTGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGa                                                                                               >  1:1094190/1‑150 (MQ=255)
                                                   gAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAg                                                                                             <  1:2265797/150‑1 (MQ=255)
                                                    aaTTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGa                                                                                            <  2:3327080/150‑1 (MQ=255)
                                                     aTTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATTGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGAc                                                                                           <  1:3549221/150‑1 (MQ=255)
                                                      tttttGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTTGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGAcc                                                                                          <  2:1588746/150‑1 (MQ=255)
                                                       ttttGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCa                                                                                         <  2:1685873/150‑1 (MQ=255)
                                                         ttGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAAt                                                                                       <  2:3400907/150‑1 (MQ=255)
                                                         ttGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAAt                                                                                       <  2:492516/150‑1 (MQ=255)
                                                          tGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATa                                                                                      >  1:3934943/1‑150 (MQ=255)
                                                              cGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACttt                                                                                  >  1:1077965/1‑150 (MQ=255)
                                                              cGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACttt                                                                                  >  2:883934/1‑150 (MQ=255)
                                                              cGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACttt                                                                                  >  1:1220102/1‑150 (MQ=255)
                                                              cGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACttt                                                                                  >  1:3887061/1‑150 (MQ=255)
                                                                aTAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCt                                                                                <  2:2394648/150‑1 (MQ=255)
                                                                  aaGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGt                                                                              >  2:2563677/1‑150 (MQ=255)
                                                                  aaGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGt                                                                              >  1:2536608/1‑150 (MQ=255)
                                                                  aaGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGt                                                                              >  2:2125907/1‑150 (MQ=255)
                                                                   aGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTGTCTGTa                                                                             >  2:4481686/1‑150 (MQ=255)
                                                                    gCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTaa                                                                            <  2:11046/150‑1 (MQ=255)
                                                                     cccACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGATTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAg                                                                           >  2:198722/1‑150 (MQ=255)
                                                                      ccACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAgg                                                                          <  2:4326267/150‑1 (MQ=255)
                                                                        aCGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTc                                                                        >  1:3529411/1‑150 (MQ=255)
                                                                         cGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCa                                                                       <  1:2919610/150‑1 (MQ=255)
                                                                          gTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCAc                                                                      <  1:3856150/150‑1 (MQ=255)
                                                                          gTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCAc                                                                      <  1:3137281/150‑1 (MQ=255)
                                                                           tttGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCAcc                                                                     <  2:3443573/150‑1 (MQ=255)
                                                                            ttGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCg                                                                    >  2:1230317/1‑150 (MQ=255)
                                                                                    ggaGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCtt                                                            <  2:2951339/148‑1 (MQ=255)
                                                                                      aGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGc                                                          >  2:4331422/1‑150 (MQ=255)
                                                                                        ccGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCgg                                                        >  2:3937409/1‑150 (MQ=255)
                                                                                         cGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGt                                                       >  2:4543810/1‑150 (MQ=255)
                                                                                            tGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGc                                                    >  2:4176509/1‑150 (MQ=255)
                                                                                            tGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGc                                                    <  1:369246/150‑1 (MQ=255)
                                                                                                 tCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGa                                               <  2:813110/150‑1 (MQ=255)
                                                                                                    tGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGaaaa                                            >  2:2156949/1‑150 (MQ=255)
                                                                                                       aCTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGt                                         <  2:1005264/150‑1 (MQ=255)
                                                                                                       aCTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGt                                         <  2:3859197/150‑1 (MQ=255)
                                                                                                       aCTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGt                                         <  2:1533150/150‑1 (MQ=255)
                                                                                                        cTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGtt                                        <  1:4505444/150‑1 (MQ=255)
                                                                                                        cTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTAGCGGTGGCGAGGAAAATGtt                                        >  2:2853264/1‑150 (MQ=255)
                                                                                                         tataTTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGttt                                       <  1:4323017/150‑1 (MQ=255)
                                                                                                         tataTTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGttt                                       <  1:2978127/150‑1 (MQ=255)
                                                                                                         tataTTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGttt                                       >  1:3492643/1‑150 (MQ=255)
                                                                                                           ttttCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTcc                                     <  2:4430284/148‑1 (MQ=255)
                                                                                                           tatTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTcc                                     >  2:25184/1‑150 (MQ=255)
                                                                                                            atTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTccc                                    >  1:2854634/1‑150 (MQ=255)
                                                                                                            atTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTGATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTACGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTccc                                    >  2:4513580/1‑150 (MQ=255)
                                                                                                              tCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCgg                                  >  2:3625772/1‑150 (MQ=255)
                                                                                                               cgcggcgcggAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGt                                 >  1:3826011/1‑150 (MQ=255)
                                                                                                                gcggcgcggAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTa                                <  1:1601340/150‑1 (MQ=255)
                                                                                                                 cggcgcggAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTaa                               <  1:1473418/150‑1 (MQ=255)
                                                                                                                  ggcgcggAGTTGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAg                              <  2:4083423/150‑1 (MQ=255)
                                                                                                                         aGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACa                       <  2:2727466/150‑1 (MQ=255)
                                                                                                                           ttGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGc                     <  1:4631229/150‑1 (MQ=255)
                                                                                                                            tGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGcc                    >  2:3095376/1‑150 (MQ=255)
                                                                                                                            tGGTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGcc                    >  2:2831660/1‑150 (MQ=255)
                                                                                                                              gTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTc                  >  1:2415938/1‑150 (MQ=255)
                                                                                                                              gTGATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTc                  <  2:2250912/150‑1 (MQ=255)
                                                                                                                                gATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGa                >  2:3992580/1‑150 (MQ=255)
                                                                                                                                gATCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGa                <  2:1426858/150‑1 (MQ=255)
                                                                                                                                 aTCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGAt               <  1:1798175/150‑1 (MQ=255)
                                                                                                                                 aTCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGAt               >  2:2480409/1‑150 (MQ=255)
                                                                                                                                  tCCATAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATg              <  1:1950829/150‑1 (MQ=255)
                                                                                                                                     aTAACGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGt           <  1:523606/150‑1 (MQ=255)
                                                                                                                                       aaCGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTa         >  1:2610198/1‑150 (MQ=255)
                                                                                                                                         cGCAGCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTAtg       <  2:2756833/150‑1 (MQ=255)
                                                                                                                                             gCGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCt   <  2:3221325/150‑1 (MQ=255)
                                                                                                                                              cGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTc  >  1:1696537/1‑150 (MQ=255)
                                                                                                                                              cGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTc  <  1:1366192/150‑1 (MQ=255)
                                                                                                                                              cGTTCTATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTc  <  1:1366158/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CCCGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTC  >  CP009273/232713‑233004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑