Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,840,562 C→A 59.1% V70V (GTG→GTT)  hycD ← hydrogenase 3, membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,840,5620CA59.1% 51.7 / inf 262V70V (GTG→GTT) hycDhydrogenase 3, membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (56/51);  new base A (83/72);  total (139/123)
Fisher's exact test for biased strand distribution p-value = 9.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.95e-01

Only 100 of 265 total aligned reads displayed.

ACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCGGGTTATACC  >  CP009273/2840415‑2840708
                                                                                                                                                   |                                                                                                                                                  
aCCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATcac                                                                                                                                                  <  2:838200/150‑1 (MQ=255)
aCCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATcac                                                                                                                                                  >  1:4383564/1‑150 (MQ=255)
   ggTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATa                                                                                                                                               >  2:912321/1‑150 (MQ=255)
   ggTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATa                                                                                                                                               >  2:3105211/1‑150 (MQ=255)
    gTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCCATAGTCAGCATGACGCCCACCATAACATAc                                                                                                                                              >  1:184243/1‑150 (MQ=255)
      aTCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACgg                                                                                                                                            >  1:1519092/1‑150 (MQ=255)
      aTCCAGACCAGAAATGGCAAAGAAGAAACGCGCGAGGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACgg                                                                                                                                            <  1:129519/150‑1 (MQ=255)
       tCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGc                                                                                                                                           <  1:3088788/150‑1 (MQ=255)
        ccagaccagaAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCg                                                                                                                                          <  1:1929897/150‑1 (MQ=255)
         cagaccagaAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGt                                                                                                                                         <  1:4010956/150‑1 (MQ=255)
         cagaccagaAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGt                                                                                                                                         >  2:4554123/1‑150 (MQ=255)
           gaccagaAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCa                                                                                                                                       <  1:3565066/150‑1 (MQ=255)
            accagaAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAg                                                                                                                                      >  1:2661694/1‑150 (MQ=255)
                gaAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCgg                                                                                                                                  >  1:319332/1‑150 (MQ=255)
                  aaTGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGaa                                                                                                                                >  2:3665990/1‑150 (MQ=255)
                   aTGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAac                                                                                                                               <  2:2176061/150‑1 (MQ=255)
                        aaaGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCa                                                                                                                          >  1:62152/1‑150 (MQ=255)
                        aaaGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCa                                                                                                                          >  2:2343431/1‑150 (MQ=255)
                        aaaGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACAGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCa                                                                                                                          >  1:62209/1‑150 (MQ=255)
                        aaaGAAGAAACGCGCGAGGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCa                                                                                                                          <  1:2485413/150‑1 (MQ=255)
                          agaagaAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGc                                                                                                                        >  2:3236623/1‑150 (MQ=255)
                           gaagaaACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGcc                                                                                                                       <  1:2082237/150‑1 (MQ=255)
                           gaagaaACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGcc                                                                                                                       >  2:1860541/1‑150 (MQ=255)
                              gaaACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGa                                                                                                                    >  1:1825939/1‑150 (MQ=255)
                               aaaCGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAg                                                                                                                   >  2:3004068/1‑150 (MQ=255)
                               aaaCGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAg                                                                                                                   <  1:4073251/150‑1 (MQ=255)
                               aaaCGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAg                                                                                                                   <  1:3475983/150‑1 (MQ=255)
                                  cgcgcgATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCa                                                                                                                >  2:2596204/1‑150 (MQ=255)
                                  cgcgcgATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCa                                                                                                                <  2:374037/150‑1 (MQ=255)
                                     gcgATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATcc                                                                                                             <  2:1166218/150‑1 (MQ=255)
                                      cgATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCg                                                                                                            >  2:3311757/1‑150 (MQ=255)
                                       gATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCgg                                                                                                           >  1:3662249/1‑150 (MQ=255)
                                          ggCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGcc                                                                                                        <  2:3835869/150‑1 (MQ=255)
                                          ggCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGAGAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGcc                                                                                                        <  2:3597637/150‑1 (MQ=255)
                                           gCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCg                                                                                                       <  2:4332476/150‑1 (MQ=255)
                                            cAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGa                                                                                                      >  2:4394108/1‑150 (MQ=255)
                                              aaGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACg                                                                                                    >  1:1617407/1‑150 (MQ=255)
                                                 agaTACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGctc                                                                                                 <  2:49164/150‑1 (MQ=255)
                                                  gaTACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGctct                                                                                                <  1:744070/150‑1 (MQ=255)
                                                   aTACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTg                                                                                               >  2:3532291/1‑150 (MQ=255)
                                                   aTACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTg                                                                                               >  2:1440695/1‑150 (MQ=255)
                                                       aGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGc                                                                                           >  1:4515051/1‑150 (MQ=255)
                                                         tAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGccc                                                                                         >  1:4604432/1‑150 (MQ=255)
                                                         tAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGccc                                                                                         >  2:3224605/1‑150 (MQ=255)
                                                         tAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGAGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGccc                                                                                         >  2:1170169/1‑150 (MQ=255)
                                                          aaGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGcccc                                                                                        <  2:1839172/150‑1 (MQ=255)
                                                           aGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCca                                                                                       >  2:3630644/1‑150 (MQ=255)
                                                            ggTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCcag                                                                                      <  1:2499054/150‑1 (MQ=255)
                                                             gTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCcagc                                                                                     >  2:3211259/1‑150 (MQ=255)
                                                               gATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCcagcag                                                                                   <  1:1182040/150‑1 (MQ=255)
                                                               gATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCcagcag                                                                                   >  1:2392799/1‑150 (MQ=255)
                                                               gATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCcagcag                                                                                   >  1:1474876/1‑150 (MQ=255)
                                                                 tCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGtt                                                                                 <  1:2864939/150‑1 (MQ=255)
                                                                 tCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGtt                                                                                 <  1:822322/150‑1 (MQ=255)
                                                                 tCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCANCCGGGCCGACGCTCTGACGCCCCAGCAGtt                                                                                 <  1:4278918/150‑1 (MQ=255)
                                                                  cAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGttt                                                                                <  1:2331597/150‑1 (MQ=255)
                                                                   aaaTCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTg                                                                               >  2:3611386/1‑150 (MQ=255)
                                                                   aaaTCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTg                                                                               >  2:4316095/1‑150 (MQ=255)
                                                                   aaaTCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTg                                                                               >  2:2455354/1‑150 (MQ=255)
                                                                    aaTCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGa                                                                              <  1:816072/150‑1 (MQ=255)
                                                                    aaTCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAACATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGa                                                                              <  1:3575925/150‑1 (MQ=255)
                                                                         cccAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATg                                                                         >  1:2822633/1‑150 (MQ=255)
                                                                           cAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTc                                                                       <  2:1136776/150‑1 (MQ=255)
                                                                             gTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTcgc                                                                     <  2:3059658/150‑1 (MQ=255)
                                                                               tGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGa                                                                   >  1:499449/1‑150 (MQ=255)
                                                                                 cGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATa                                                                 >  1:382406/1‑150 (MQ=255)
                                                                                 cGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATa                                                                 <  2:4108477/150‑1 (MQ=255)
                                                                                 cGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATa                                                                 <  2:1778207/150‑1 (MQ=255)
                                                                                   gCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACt                                                               <  2:4486507/150‑1 (MQ=255)
                                                                                   gCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACt                                                               <  2:3469665/150‑1 (MQ=255)
                                                                                      gCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCt                                                            <  1:3273977/150‑1 (MQ=255)
                                                                                       cGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTg                                                           >  1:1356190/1‑150 (MQ=255)
                                                                                        ggAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGc                                                          >  1:550556/1‑150 (MQ=255)
                                                                                           gaACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAac                                                       >  1:3029155/1‑150 (MQ=255)
                                                                                                  cGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACgcccg                                                <  1:390135/150‑1 (MQ=255)
                                                                                                      caccacCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACgcccggccc                                            <  2:3135834/150‑1 (MQ=255)
                                                                                                           ccGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGAc                                       <  2:848995/150‑1 (MQ=255)
                                                                                                             ggCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACgg                                     >  1:1851608/1‑150 (MQ=255)
                                                                                                                 gcgcAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTAt                                 <  2:4233882/150‑1 (MQ=255)
                                                                                                                  cgcAGTAGCGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATg                                >  1:2860152/1‑150 (MQ=255)
                                                                                                                       tAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGc                           <  2:53618/150‑1 (MQ=255)
                                                                                                                        aGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCg                          <  2:2737235/150‑1 (MQ=255)
                                                                                                                          cGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCggg                        >  1:4118295/1‑150 (MQ=255)
                                                                                                                          cGATAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCggg                        >  2:1920897/1‑150 (MQ=255)
                                                                                                                            aTAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGgcg                      <  1:263804/150‑1 (MQ=255)
                                                                                                                             tAGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGgcgc                     >  2:1376795/1‑150 (MQ=255)
                                                                                                                              aGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTGATGCAAGCGGgcgcg                    >  2:3984055/1‑150 (MQ=255)
                                                                                                                              aGTCAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGgcgcg                    <  2:1972414/150‑1 (MQ=255)
                                                                                                                                 cAGCATGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGgcgcgcgc                 >  1:2184603/1‑150 (MQ=255)
                                                                                                                                  aGCATGACGCCCTCCATAACATACGGCGTCAGGCGGAACACCCAGCCGGTGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGgcgcgcgac                <  2:3415121/150‑3 (MQ=255)
                                                                                                                                     aTGACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCAcg             <  1:1443974/150‑1 (MQ=255)
                                                                                                                                      tGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCAcgc            >  2:2571764/1‑150 (MQ=255)
                                                                                                                                      tGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCAcgc            >  2:2962871/1‑150 (MQ=255)
                                                                                                                                      tGACGCCCACCATCACATAAGGCGTCAGGCTGAACACCCAGCCGGAGGCATCAGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCAcgc            >  2:2058573/1‑150 (MQ=255)
                                                                                                                                       gACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCAcgcg           >  2:2765269/1‑150 (MQ=255)
                                                                                                                                       gACGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCAcgcg           <  1:3311758/150‑1 (MQ=255)
                                                                                                                                         cGCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCggg         >  2:1674871/1‑150 (MQ=255)
                                                                                                                                          gCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCGGGt        >  2:4219742/1‑150 (MQ=255)
                                                                                                                                          gCCCACCATAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCGGGt        >  2:2882039/1‑150 (MQ=255)
                                                                                                                                               ccaTAACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCGGGTTATAc   <  2:1028454/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGATAGTCAGCATGACGCCCACCATCACATACGGCGTCAGGCGGAACACCCAGCCGGAGGCATCCGGGCCGACGCTCTGACGCCCCAGCAGTTTGATAATGTCGCGATACTCCTGCAACACGCCCGGCCCGCGACGGTTATGCAAGCGGGCGCGCGCCACGCGGGTTATACC  >  CP009273/2840415‑2840708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑