Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,888,584 G→T 100% I185I (ATC→ATA)  ygcQ ← putative flavoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,888,5840GT95.8% 1060.5 / 34.6 330I185I (ATC→ATA) ygcQputative flavoprotein
Reads supporting (aligned to +/- strand):  ref base G (9/5);  new base T (179/137);  total (188/142)
Fisher's exact test for biased strand distribution p-value = 7.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 330 total aligned reads displayed.

CTGCGCCAGAAGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGTTGCAT  >  CP009273/2888436‑2888730
                                                                                                                                                    |                                                                                                                                                  
   cgcCAGAAGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTAttt                                                                                                                                                <  2:1814688/150‑1 (MQ=255)
    gcCAGAAGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTc                                                                                                                                               <  2:1618336/150‑1 (MQ=255)
    gcCAGAAGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTc                                                                                                                                               <  2:2457501/150‑1 (MQ=255)
     ccAGAAGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTcc                                                                                                                                              >  2:784075/1‑150 (MQ=255)
      cAGAAGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCt                                                                                                                                             <  1:3134247/150‑1 (MQ=255)
      cAGAAGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCt                                                                                                                                             <  1:1988579/150‑1 (MQ=255)
       aGAAGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTg                                                                                                                                            <  1:4456957/150‑1 (MQ=255)
        gAAGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTgg                                                                                                                                           >  2:4210520/1‑150 (MQ=255)
         aaGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGt                                                                                                                                          >  2:3492591/1‑150 (MQ=255)
         aaGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGt                                                                                                                                          >  1:822700/1‑150 (MQ=255)
         aaGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGt                                                                                                                                          <  1:701566/150‑1 (MQ=255)
         aaGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGt                                                                                                                                          <  2:2266423/150‑1 (MQ=255)
         aaGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGt                                                                                                                                          <  2:60126/150‑1 (MQ=255)
          aGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGtt                                                                                                                                         >  1:2640850/1‑150 (MQ=255)
           gcgcCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTa                                                                                                                                        <  2:2786050/150‑1 (MQ=255)
             gcCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATc                                                                                                                                      >  2:4232854/1‑150 (MQ=255)
             gcCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATc                                                                                                                                      <  1:3172275/150‑1 (MQ=255)
             gcCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATc                                                                                                                                      <  2:557850/150‑1 (MQ=255)
               caacaacaaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTg                                                                                                                                    >  1:1846885/1‑150 (MQ=255)
                aacaacaaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGc                                                                                                                                   >  1:3297257/1‑150 (MQ=255)
                aacaacaaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGc                                                                                                                                   >  2:2555492/1‑150 (MQ=255)
                aacaacaaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGc                                                                                                                                   >  1:3704321/1‑150 (MQ=255)
                  caacaaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCct                                                                                                                                 >  1:901637/1‑150 (MQ=255)
                   aacaaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCctc                                                                                                                                <  2:4507915/150‑1 (MQ=255)
                    acaaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCctct                                                                                                                               <  2:2534209/150‑1 (MQ=255)
                     caaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCATTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCCtctc                                                                                                                              >  2:704061/1‑150 (MQ=255)
                      aaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTcc                                                                                                                             >  1:437238/1‑150 (MQ=255)
                      aaTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTcc                                                                                                                             <  2:2890674/150‑1 (MQ=255)
                       aTACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCg                                                                                                                            <  2:3907320/150‑1 (MQ=255)
                        tACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGc                                                                                                                           >  1:1631847/1‑150 (MQ=255)
                        tACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGc                                                                                                                           >  1:1360583/1‑150 (MQ=255)
                         acacacCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGcc                                                                                                                          <  2:2300379/150‑1 (MQ=255)
                          cacacCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGccc                                                                                                                         <  2:3301047/150‑1 (MQ=255)
                            cacCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTg                                                                                                                       <  2:1349511/150‑1 (MQ=255)
                              ccTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGcc                                                                                                                     >  1:347179/1‑150 (MQ=255)
                                  aGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCccac                                                                                                                 >  1:2859242/1‑150 (MQ=255)
                                    gTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCccacca                                                                                                               >  1:3095503/1‑150 (MQ=255)
                                      gCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGCCCTGCCccaccacc                                                                                                             <  1:1005595/150‑1 (MQ=255)
                                       ccAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCccaccacca                                                                                                            <  1:4045490/150‑1 (MQ=255)
                                       ccAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCccaccacca                                                                                                            >  1:1240696/1‑150 (MQ=255)
                                        cagcagATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAg                                                                                                           >  2:874294/1‑150 (MQ=255)
                                           cagATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCAc                                                                                                        >  1:1129102/1‑150 (MQ=255)
                                           cagATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCAc                                                                                                        >  1:3007256/1‑150 (MQ=255)
                                            agATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCAcc                                                                                                       >  2:4031259/1‑150 (MQ=255)
                                            agATGTCCGGAAAGACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCAcc                                                                                                       >  1:2902416/1‑150 (MQ=255)
                                                 tCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCt                                                                                                  >  1:156298/1‑150 (MQ=255)
                                                    ggAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGc                                                                                               >  2:3412043/1‑150 (MQ=255)
                                                    ggAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACAACCAGCACCCGTCTGGc                                                                                               >  1:2571744/1‑150 (MQ=255)
                                                      aaaTACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCt                                                                                             >  1:2553331/1‑150 (MQ=255)
                                                      aaaTACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCt                                                                                             >  2:1999946/1‑150 (MQ=255)
                                                       aaTACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTc                                                                                            <  2:3182188/150‑1 (MQ=255)
                                                        aTACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCa                                                                                           >  2:3611871/1‑150 (MQ=255)
                                                        aTACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCa                                                                                           <  2:2116000/150‑1 (MQ=255)
                                                          aCCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGc                                                                                         <  2:18857/150‑1 (MQ=255)
                                                          aCCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCACCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGc                                                                                         >  2:2738124/1‑150 (MQ=255)
                                                              aTCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCTGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAAt                                                                                     <  2:2856845/150‑1 (MQ=255)
                                                                cACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATgg                                                                                   <  2:2400958/150‑1 (MQ=255)
                                                                       tCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCgg                                                                            <  2:4294751/150‑1 (MQ=255)
                                                                           cATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTaa                                                                        <  1:1463802/150‑1 (MQ=255)
                                                                           cATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTaa                                                                        >  2:437220/1‑150 (MQ=255)
                                                                              cAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACAt                                                                     >  1:17897/1‑150 (MQ=255)
                                                                              cAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACAt                                                                     >  2:310974/1‑150 (MQ=255)
                                                                               aaCGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACAtt                                                                    >  1:508860/1‑150 (MQ=255)
                                                                                aCGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACAttt                                                                   <  1:2114716/150‑1 (MQ=255)
                                                                                  gCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACAttttt                                                                 >  2:2078866/1‑150 (MQ=255)
                                                                                   ccACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTc                                                                >  1:210129/1‑150 (MQ=255)
                                                                                    cACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCa                                                               >  2:162950/1‑150 (MQ=255)
                                                                                     aCCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAg                                                              >  1:1817345/1‑150 (MQ=255)
                                                                                     aCCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAg                                                              <  2:111742/150‑1 (MQ=255)
                                                                                        gTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACAACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATc                                                           >  2:1502608/1‑150 (MQ=255)
                                                                                         ttCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATcc                                                          >  2:63881/1‑150 (MQ=255)
                                                                                            atcaCTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCctct                                                       <  1:740600/150‑1 (MQ=255)
                                                                                            atcaCTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCctct                                                       >  1:3862615/1‑150 (MQ=255)
                                                                                                    cgcCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAAc                                               <  1:1376088/150‑1 (MQ=255)
                                                                                                      cccGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAAcca                                             <  1:281906/150‑1 (MQ=255)
                                                                                                         gACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAAccagcc                                          >  2:3733097/1‑150 (MQ=255)
                                                                                                         gACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAAccagcc                                          >  2:2104181/1‑150 (MQ=255)
                                                                                                           cTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAAccagccag                                        >  1:2286345/1‑150 (MQ=255)
                                                                                                            tGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGt                                       >  2:3798078/1‑150 (MQ=255)
                                                                                                               aGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCTTCTGTGCTAACCAGCCAGTCCg                                    <  1:460632/150‑1 (MQ=255)
                                                                                                                 cccACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGa                                  >  2:908673/1‑150 (MQ=255)
                                                                                                                  ccACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGaa                                 <  2:1914882/150‑1 (MQ=255)
                                                                                                                      ttCGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAgcgc                             <  2:3327151/150‑1 (MQ=255)
                                                                                                                        cGGCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCAc                           <  1:636514/150‑1 (MQ=255)
                                                                                                                         ggCTCCCAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCAcc                          >  1:4298989/1‑150 (MQ=255)
                                                                                                                               cAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGAc                    <  1:1275370/150‑1 (MQ=255)
                                                                                                                               cAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGAc                    <  1:4232854/150‑1 (MQ=255)
                                                                                                                               cAGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGAc                    >  1:489339/1‑150 (MQ=255)
                                                                                                                                aGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACa                   >  2:2253098/1‑150 (MQ=255)
                                                                                                                                aGCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACa                   >  2:4597929/1‑150 (MQ=255)
                                                                                                                                 gCTTTTCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACaa                  <  1:1015848/150‑1 (MQ=255)
                                                                                                                                  cTTTTCTGCCAGCAGAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAAt                 <  2:1690301/150‑1 (MQ=255)
                                                                                                                                   ttttCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATg                >  1:3495164/1‑150 (MQ=255)
                                                                                                                                     ttCTGCCAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGtt              >  2:4404007/1‑150 (MQ=255)
                                                                                                                                          ccAGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAgtt         >  2:503479/1‑150 (MQ=255)
                                                                                                                                            aGCATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGACGCGCACCCGGGACAATGTTCAgttgt       >  2:1262268/1‑150 (MQ=255)
                                                                                                                                              cATAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCCCCCGGGACAATGTTCAGttgttg     >  1:4126542/1‑150 (MQ=255)
                                                                                                                                               aTAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGTTGc    >  1:4220037/1‑150 (MQ=255)
                                                                                                                                               aTAGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGTTGc    >  1:2471631/1‑150 (MQ=255)
                                                                                                                                                 aGCTATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGTTGCAt  >  2:724635/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CTGCGCCAGAAGCGCCAACAACAATACACACCTCAGGTGCCAGCAGATGTCCGGAAATACCAATCACTTTTTCAGCATCAACGCCACCGTTCATCACTCGCGCCCGACTGTAGCCCACTTCGGCTCCCAGCTTTTCTGCCAGCATAGCGATTTCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGTTGCAT  >  CP009273/2888436‑2888730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑