BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,950,594	G→T	36.1%	V550V (GTC→GTA)	ptrA ←	protease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,950,594	0	G	T	36.1%	513.4 / inf	321	V550V (GTC→GTA)	ptrA	protease III
Reads supporting (aligned to +/- strand): ref base G (106/99); new base T (66/50); total (172/149)
Fisher's exact test for biased strand distribution p-value = 4.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 323 total aligned reads displayed.

TGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGG  >  CP009273/2950446‑2950743

tgttAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGa                                                                                                                                                      >  1:1166009/1‑150 (MQ=255)

 gttAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGAc                                                                                                                                                     >  1:2500515/1‑150 (MQ=255)

 gttAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGAc                                                                                                                                                     >  1:538574/1‑150 (MQ=255)

   tAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACAt                                                                                                                                                   >  2:179677/1‑150 (MQ=255)

   tAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACAt                                                                                                                                                   >  2:3575214/1‑150 (MQ=255)

       gTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGc                                                                                                                                               >  2:1501907/1‑150 (MQ=255)

         tggtggAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCtt                                                                                                                                             >  2:522078/1‑150 (MQ=255)

           gtggAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCCCCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTg                                                                                                                                           <  2:869334/150‑1 (MQ=255)

            tggAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTgg                                                                                                                                          <  2:3058042/150‑1 (MQ=255)

                aaaaCTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTc                                                                                                                                      <  1:739831/150‑1 (MQ=255)

                     ttATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTgg                                                                                                                                 >  2:1602670/1‑150 (MQ=255)

                      tATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGc                                                                                                                                <  2:3974688/150‑1 (MQ=255)

                           caccaACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAAt                                                                                                                           >  2:18573/1‑150 (MQ=255)

                            accaACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATa                                                                                                                          >  2:241102/1‑150 (MQ=255)

                             ccaACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATaa                                                                                                                         >  2:4417315/1‑150 (MQ=255)

                              caACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAAc                                                                                                                        <  1:3558720/150‑1 (MQ=255)

                               aaCCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACg                                                                                                                       <  2:1269120/150‑1 (MQ=255)

                                aCCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACgg                                                                                                                      >  1:3315996/1‑150 (MQ=255)

                                aCCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACgg                                                                                                                      >  1:3316044/1‑150 (MQ=255)

                                 ccGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGc                                                                                                                     >  2:2257904/1‑150 (MQ=255)

                                  cGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCt                                                                                                                    <  1:4035891/150‑1 (MQ=255)

                                  cGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCt                                                                                                                    <  1:1103858/150‑1 (MQ=255)

                                   gACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCtt                                                                                                                   >  1:1585709/1‑150 (MQ=255)

                                    aCGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTg                                                                                                                  <  1:124758/150‑1 (MQ=255)

                                       ccTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGgcg                                                                                                               >  2:3680317/1‑150 (MQ=255)

                                       ccTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTGTGGGCTCGCTGGCAAAATAACGGCTTGgcg                                                                                                               >  2:3680881/1‑150 (MQ=255)

                                        cTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGgcgc                                                                                                              >  1:3640576/1‑150 (MQ=255)

                                        cTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGgcgc                                                                                                              >  1:3178990/1‑150 (MQ=255)

                                              gCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATacac                                                                                                        >  1:4331036/1‑150 (MQ=255)

                                                ttAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATAcacca                                                                                                      <  2:2076374/150‑1 (MQ=255)

                                                 tAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATAcaccac                                                                                                     <  1:1456589/150‑1 (MQ=255)

                                                  aaCTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCAcg                                                                                                    >  1:2123463/1‑150 (MQ=255)

                                                  aaCTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCAcg                                                                                                    >  1:4088592/1‑150 (MQ=255)

                                                  aaCTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCAcg                                                                                                    >  1:293455/1‑150 (MQ=255)

                                                  aaCTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCAcg                                                                                                    >  1:419660/1‑150 (MQ=255)

                                                      gATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCa                                                                                                <  2:3783978/150‑1 (MQ=255)

                                                       aTCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAg                                                                                               <  1:353288/150‑1 (MQ=255)

                                                        tCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTGTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGa                                                                                              >  2:1826658/1‑150 (MQ=255)

                                                          aaGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGAtt                                                                                            <  1:4341426/150‑1 (MQ=255)

                                                                 aGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACtca                                                                                     >  2:2579233/1‑150 (MQ=255)

                                                                  gCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACtcat                                                                                    <  1:3069249/150‑1 (MQ=255)

                                                                   cccTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACtcatc                                                                                   <  1:1302784/150‑1 (MQ=255)

                                                                   cccTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACtcatc                                                                                   >  1:4633688/1‑150 (MQ=255)

                                                                   cccTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACtcatc                                                                                   >  1:2298759/1‑150 (MQ=255)

                                                                    ccTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACtcatca                                                                                  >  2:4542352/1‑150 (MQ=255)

                                                                      tGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATcaac                                                                                >  1:3481353/1‑150 (MQ=255)

                                                                        cGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATcaacaa                                                                              >  2:4602186/1‑150 (MQ=255)

                                                                        cGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATcaacaa                                                                              >  2:1427290/1‑150 (MQ=255)

                                                                         gagaTAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAAt                                                                             <  2:864845/150‑1 (MQ=255)

                                                                           gaTAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCATAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCa                                                                           <  1:4591186/150‑1 (MQ=255)

                                                                           gaTAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGTAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCa                                                                           >  1:3935489/1‑150 (MQ=255)

                                                                            aTAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAg                                                                          >  1:2907045/1‑150 (MQ=255)

                                                                             tAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGc                                                                         >  2:4438613/1‑150 (MQ=255)

                                                                                tCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGctct                                                                      <  2:1614140/150‑1 (MQ=255)

                                                                                    tGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGAt                                                                  >  1:3833877/1‑150 (MQ=255)

                                                                                        cgcAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTc                                                              <  1:1060737/150‑1 (MQ=255)

                                                                                          cAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGt                                                            <  1:3418490/150‑1 (MQ=255)

                                                                                           aaaCATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCTTa                                                           >  2:1758582/1‑150 (MQ=255)

                                                                                           aaaCATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTa                                                           >  2:4089352/1‑150 (MQ=255)

                                                                                              cATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTAttt                                                        >  2:3759472/1‑150 (MQ=255)

                                                                                                 cACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATttctt                                                     <  1:236131/150‑1 (MQ=255)

                                                                                                  aCCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATttcttc                                                    >  2:254056/1‑150 (MQ=255)

                                                                                                   ccTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTtcttct                                                   <  1:713603/150‑1 (MQ=255)

                                                                                                     tGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTctct                                                 <  1:2073120/150‑1 (MQ=255)

                                                                                                      gATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTg                                                >  2:574190/1‑150 (MQ=255)

                                                                                                      gATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTTGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGAATCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTg                                                <  1:1316549/150‑1 (MQ=255)

                                                                                                      gATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTg                                                <  1:50167/150‑1 (MQ=255)

                                                                                                       aTTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGa                                               >  1:3746422/1‑150 (MQ=255)

                                                                                                        ttGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGAc                                              <  2:25101/150‑1 (MQ=255)

                                                                                                        ttGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGAc                                              <  2:293455/150‑1 (MQ=255)

                                                                                                           cGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTa                                           >  2:1167963/1‑150 (MQ=255)

                                                                                                            gggCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTaa                                          <  1:2240763/150‑1 (MQ=255)

                                                                                                            gcgcgcTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTaa                                          >  1:170/3‑150 (MQ=255)

                                                                                                                gcTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAg                                      <  2:804496/150‑1 (MQ=255)

                                                                                                                gcTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAg                                      <  2:1363020/150‑1 (MQ=255)

                                                                                                                gcTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAg                                      >  2:1331117/1‑150 (MQ=255)

                                                                                                                gcTGACCATGGCTTTCTGATTAATCAAAATCAGGCTTAAATCAGCTTTGGGCACGCTGGAAAAATAACGGCTTGGCGCATACACCACGCGCACATTCGACTCAACAACAAACAGCACTGGATGGACGTATTTCTTCACTGACTAAATCAg                                      >  2:2137073/1‑150 (MQ=255)

                                                                                                                    tCCATGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCgag                                  >  1:310550/1‑150 (MQ=255)

                                                                                                                        tGGCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAat                              >  2:1594448/1‑150 (MQ=255)

                                                                                                                         ggCTTTCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAatc                             <  2:4331220/150‑1 (MQ=255)

                                                                                                                         ggCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAatc                             <  1:447892/150‑1 (MQ=255)

                                                                                                                          gCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAatca                            >  1:1009237/1‑150 (MQ=255)

                                                                                                                          gCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAatca                            >  1:3658745/1‑150 (MQ=255)

                                                                                                                           cTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAatcat                           <  2:1110882/150‑1 (MQ=255)

                                                                                                                             ttCGGATTACGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAAtcatca                         >  1:1891791/1‑150 (MQ=255)

                                                                                                                               cGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAgg                       >  2:382782/1‑150 (MQ=255)

                                                                                                                                  aTTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAat                    >  1:1179835/1‑150 (MQ=255)

                                                                                                                                      cGCAAAATCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATaa                <  1:1382059/150‑1 (MQ=255)

                                                                                                                                      cGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATaa                >  1:2029384/1‑150 (MQ=255)

                                                                                                                                      cGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATaa                >  2:3430748/1‑150 (MQ=255)

                                                                                                                                      cGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATaa                <  2:2311533/150‑1 (MQ=255)

                                                                                                                                      cGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATaa                >  2:569454/1‑150 (MQ=255)

                                                                                                                                       gCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAg               <  2:4234945/150‑1 (MQ=255)

                                                                                                                                           aaTCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGtt           <  2:708576/150‑1 (MQ=255)

                                                                                                                                             tCAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTaa         >  2:2000241/1‑150 (MQ=255)

                                                                                                                                             tCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTaa         >  2:2690377/1‑150 (MQ=255)

                                                                                                                                             tCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTaa         >  1:915400/1‑150 (MQ=255)

                                                                                                                                              cAGGCTTACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAg        <  1:2156565/150‑1 (MQ=255)

                                                                                                                                                ggCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGct      <  1:18573/150‑1 (MQ=255)

                                                                                                                                                    gACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTgg  >  2:2970631/1‑150 (MQ=255)

TGTTAGCGTTGGTGGAAAAACTTATGCCACCAACCGACGCCTGGTTGCTTAACTGATCAAGCGCCAGCCCTGCGAGATAATCATTGAGCGCAAACATCACCTGATTGCGGGCGCTGTCCATGGCTTTCGGATTACGCAAAATCAGGCTGACATCAGCTTTGGGCTCGCTGGCAAAATAACGGCTTGGCGCATACACCACGCGCAGATTCGACTCATCAACAATCAGCTCTGGATGGTCGTATTTCTTCTCTGACTTAATCAGCGAGAAATCATCAGGAATATAAGGGTTAAGCTCTGG  >  CP009273/2950446‑2950743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`