BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,979,222	C→T	18.4%	S6F (TCT→TTT)	yqeG →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,979,222	0	C	T	18.4%	647.7 / 119.6	250	S6F (TCT→TTT)	yqeG	putative transporter
Reads supporting (aligned to +/- strand): ref base C (97/107); new base T (21/25); total (118/132)
Fisher's exact test for biased strand distribution p-value = 8.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 254 total aligned reads displayed.

ACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCTTTAACATATTGGCCACATA  >  CP009273/2979074‑2979371

aCTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCt                                                                                                                                                      <  2:3703562/150‑1 (MQ=255)

aCTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCt                                                                                                                                                      <  2:1322385/150‑1 (MQ=255)

 cTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTa                                                                                                                                                     <  1:2912646/150‑1 (MQ=255)

 cTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTa                                                                                                                                                     <  1:1007035/150‑1 (MQ=255)

      aCGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAaagaa                                                                                                                                                >  1:4405241/1‑150 (MQ=255)

      aCGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAaagaa                                                                                                                                                >  1:3272799/1‑150 (MQ=255)

             gTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCT‑CATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAActct                                                                                                                                        >  1:1278660/1‑150 (MQ=255)

              tttGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAActct                                                                                                                                        >  2:180097/1‑150 (MQ=255)

                tGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCtgt                                                                                                                                      <  2:3465019/150‑1 (MQ=255)

                 gCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCtgtg                                                                                                                                     >  2:2364191/1‑150 (MQ=255)

                   aaCCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGa                                                                                                                                   <  2:3390295/150‑1 (MQ=255)

                   aaCCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGa                                                                                                                                   <  1:1450986/150‑1 (MQ=255)

                        cTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTc                                                                                                                              <  2:628877/150‑1 (MQ=255)

                        cTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTc                                                                                                                              >  2:3197689/1‑150 (MQ=255)

                         tAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTGTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTcg                                                                                                                             >  2:1494743/1‑150 (MQ=255)

                             ccaccaATCGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGct                                                                                                                         <  1:576741/150‑1 (MQ=255)

                             ccaccaATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGct                                                                                                                         <  2:3487769/150‑1 (MQ=255)

                              caccaATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGctc                                                                                                                        >  1:753067/1‑150 (MQ=255)

                              caccaATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGctc                                                                                                                        <  2:1830020/150‑1 (MQ=255)

                               cccaATAGAGGGGTAGATAGGGCGTTAATCTCCCATACCTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGctct                                                                                                                       <  2:2044/149‑1 (MQ=255)

                               accaATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTTTAAAGAAGAAACTCTGTGGAGTTTCGCGctct                                                                                                                       <  2:2782348/150‑1 (MQ=255)

                               accaATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGctct                                                                                                                       <  1:1877691/150‑1 (MQ=255)

                               accaATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGctct                                                                                                                       >  1:1287047/1‑150 (MQ=255)

                               accaATAGAGGGGTAGATAGGGCGTTAATCTCCCATACCTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGctct                                                                                                                       <  2:1925/150‑1 (MQ=255)

                               accaATAGAGGGGTAGAGAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGctct                                                                                                                       <  2:2260647/150‑1 (MQ=255)

                                ccaATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTa                                                                                                                      >  2:796519/1‑150 (MQ=255)

                                     agagGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGca                                                                                                                 >  2:4017266/1‑150 (MQ=255)

                                      gagGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGcac                                                                                                                <  2:2641184/150‑1 (MQ=255)

                                       agGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTTTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGcaca                                                                                                               >  1:1548032/1‑150 (MQ=255)

                                        ggggTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAg                                                                                                              <  1:200124/150‑1 (MQ=255)

                                        ggggTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAg                                                                                                              >  2:735007/1‑150 (MQ=255)

                                          ggTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGcc                                                                                                            <  2:4326119/150‑1 (MQ=255)

                                             aGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGtt                                                                                                         <  1:1019544/150‑1 (MQ=255)

                                                   ggCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCa                                                                                                   <  1:906412/150‑1 (MQ=255)

                                                     cGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTTTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAgg                                                                                                 <  2:3486977/150‑1 (MQ=255)

                                                     cGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAgg                                                                                                 <  1:2250404/150‑1 (MQ=255)

                                                      gTTAATCTCCCTTCCTTATCATGGTTTATTGTAAATTGCCCTCCTTTTTGTTTAATTTGTAGATTATTCGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGACACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGc                                                                                                <  2:770441/150‑1 (MQ=255)

                                                         aaTCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACg                                                                                             >  1:4303811/1‑150 (MQ=255)

                                                                cATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTcttcc                                                                                      >  1:4500158/1‑150 (MQ=255)

                                                                 aTACTTACCCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTTTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTcttcct                                                                                     <  2:2545208/150‑1 (MQ=255)

                                                                          cTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCa                                                                            >  2:3940085/1‑150 (MQ=255)

                                                                            ggTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGt                                                                          <  1:4063105/150‑1 (MQ=255)

                                                                            ggTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGt                                                                          >  2:2985728/1‑150 (MQ=255)

                                                                               ttATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTTTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAg                                                                       <  1:1488202/150‑1 (MQ=255)

                                                                               ttATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTTTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAg                                                                       <  1:1670214/150‑1 (MQ=255)

                                                                                tATGGTAAATTGCCCTCCATTTTGTTTAATTAGTAGATGATACGTTCAGATAATGTCTAATAATTGGTCTAAAGAAGAAACTCTGTGTAGTTTCGCGCTCTACGGCACAGCAGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAgg                                                                      >  2:1816318/1‑150 (MQ=255)

                                                                                 aTGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGt                                                                     >  2:2816550/1‑150 (MQ=255)

                                                                                   ggTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTc                                                                   <  1:630094/150‑1 (MQ=255)

                                                                                   ggTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTc                                                                   <  2:710358/150‑1 (MQ=255)

                                                                                    gTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCg                                                                  >  2:1636306/1‑150 (MQ=255)

                                                                                     tAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCgg                                                                 >  1:2722378/1‑150 (MQ=255)

                                                                                      aaaTTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGc                                                                >  1:396957/1‑150 (MQ=255)

                                                                                       aaTTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCa                                                               >  1:1231068/1‑150 (MQ=255)

                                                                                        aTTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAg                                                              <  2:3840843/150‑1 (MQ=255)

                                                                                             ccTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCt                                                         <  1:3520365/150‑1 (MQ=255)

                                                                                             ccTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCt                                                         >  2:3107723/1‑150 (MQ=255)

                                                                                               tCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCtgt                                                       <  1:4189655/150‑1 (MQ=255)

                                                                                                ccATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCtgtg                                                      <  2:1755283/150‑1 (MQ=255)

                                                                                                ccATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCtgtg                                                      <  1:522539/150‑1 (MQ=255)

                                                                                                 cATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTATAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTgg                                                     <  2:3291537/150‑1 (MQ=255)

                                                                                                  aTTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTTTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGt                                                    >  1:3341679/1‑150 (MQ=255)

                                                                                                   ttttGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTc                                                   >  1:2845477/1‑150 (MQ=255)

                                                                                                     ttGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTTGGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCt                                                 <  2:3793464/150‑1 (MQ=255)

                                                                                                     ttGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCt                                                 <  2:4082103/150‑1 (MQ=255)

                                                                                                     ttGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCt                                                 <  2:2811754/150‑1 (MQ=255)

                                                                                                       gTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGt                                               >  2:2448358/1‑150 (MQ=255)

                                                                                                       gTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGt                                               >  2:1355481/1‑150 (MQ=255)

                                                                                                        tttAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGtt                                              >  1:3203078/1‑150 (MQ=255)

                                                                                                         ttAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGttt                                             <  1:3841402/150‑1 (MQ=255)

                                                                                                         ttAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGttt                                             <  1:1743066/150‑1 (MQ=255)

                                                                                                            aTTTGTAGATGATACGTTCAGATCATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTttatt                                          >  2:2411393/1‑150 (MQ=255)

                                                                                                            aTTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAg                                                              <  2:1256844/130‑1 (MQ=255)

                                                                                                                gTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTg                                      <  1:2364191/150‑1 (MQ=255)

                                                                                                                  aGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGct                                    <  1:2419741/150‑1 (MQ=255)

                                                                                                                  aGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGct                                    <  1:125235/150‑1 (MQ=255)

                                                                                                                  aGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGct                                    <  1:4301039/150‑1 (MQ=255)

                                                                                                                   gatgatACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGctc                                   >  2:3583909/1‑150 (MQ=255)

                                                                                                                       atACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGt                               >  2:4533314/1‑150 (MQ=255)

                                                                                                                       atACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGt                               >  2:3235527/1‑150 (MQ=255)

                                                                                                                        tACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTc                              <  2:2205262/150‑1 (MQ=255)

                                                                                                                            ttCAGATAATGTCTAATATTTGGTTTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCt                          <  2:4196503/150‑1 (MQ=255)

                                                                                                                            ttCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCt                          >  1:2522021/1‑150 (MQ=255)

                                                                                                                            ttCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCt                          <  1:982822/150‑1 (MQ=255)

                                                                                                                             tCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTg                         <  2:4633343/150‑1 (MQ=255)

                                                                                                                             tCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGAACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTAATGCTCTGGTCGCCTg                         >  1:3579840/1‑150 (MQ=255)

                                                                                                                                 aTAATGTCTAATATTTGGTTTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCt                     <  1:3744237/150‑1 (MQ=255)

                                                                                                                                 aTAATGTCTAATATTTGGTTTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCt                     >  1:511873/1‑150 (MQ=255)

                                                                                                                                   aaTGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCttt                   <  1:1584616/150‑1 (MQ=255)

                                                                                                                                    aTGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCTTTa                  >  2:3050486/1‑150 (MQ=255)

                                                                                                                                     tGTCTAATATTTGGTTTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCTTTaa                 >  2:4345448/1‑150 (MQ=255)

                                                                                                                                      gTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCTCGCTCTACGGCACAGCCGATGGTGCATGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGTTCGCCTTGCCTTTAAc                >  2:1430415/1‑150 (MQ=255)

                                                                                                                                       tCTAATATTTGGTTTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCTTTAACa               >  1:1973415/1‑150 (MQ=255)

                                                                                                                                        cTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCTTTAACat              <  2:3079627/150‑1 (MQ=255)

                                                                                                                                         tAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCTTTAACata             <  2:914150/150‑1 (MQ=255)

                                                                                                                                           atatTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCTTTAACATAtt           <  2:3984568/150‑1 (MQ=255)

                                                                                                                                            tatTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCTTTAACATATTg          >  2:3243853/1‑150 (MQ=255)

                                                                                                                                                 ggTTTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCTTTAACATATTGGCcac     >  2:2334548/1‑150 (MQ=255)

                                                                                                                                                 ggTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCTTTAACATATTGGCcac     >  2:939100/1‑150 (MQ=255)

                                                                                                                                                    cTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCTTTAACATATTGGCCACATa  >  1:474663/1‑150 (MQ=255)

                                                                                                                                                    cTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCTTTAACATATTGGCCACATa  >  2:3033394/1‑150 (MQ=255)

ACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCTTTAACATATTGGCCACATA  >  CP009273/2979074‑2979371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`