BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,021,141	T→C	16.2%	I221T (ATC→ACC)	ygfQ →	putative purine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,021,141	0	T	C	16.2%	658.3 / 116.8	261	I221T (ATC→ACC)	ygfQ	putative purine permease
Reads supporting (aligned to +/- strand): ref base T (111/107); new base C (19/23); total (131/130)
Fisher's exact test for biased strand distribution p-value = 6.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01

Only 100 of 275 total aligned reads displayed.

CAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGG  >  CP009273/3020992‑3021290

cAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGat                                                                                                                                                       >  2:3351265/1‑150 (MQ=255)

 aaaaaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGatc                                                                                                                                                      <  2:4001974/150‑1 (MQ=255)

    aaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGatcatc                                                                                                                                                   <  1:1227751/150‑1 (MQ=255)

        cGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCt                                                                                                                                               <  1:3314606/150‑1 (MQ=255)

         gATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCtt                                                                                                                                              >  1:298308/1‑150 (MQ=255)

            tGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAAt                                                                                                                                           >  2:1894178/1‑150 (MQ=255)

               aGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCtt                                                                                                                                        >  1:2058642/1‑150 (MQ=255)

                ggCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCttt                                                                                                                                       <  1:4546056/150‑1 (MQ=255)

                ggCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCttt                                                                                                                                       <  1:727138/150‑1 (MQ=255)

                 gCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCGTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTg                                                                                                                                      <  2:4429252/150‑1 (MQ=255)

                   ttGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGAc                                                                                                                                    <  1:1585237/150‑1 (MQ=255)

                   ttGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGGTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGAc                                                                                                                                    >  2:1242003/1‑150 (MQ=255)

                     gCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGAccc                                                                                                                                  >  1:2644397/1‑150 (MQ=255)

                        aGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGACCATCGGCTTAATCTTTGACCCAGc                                                                                                                               >  1:411393/1‑150 (MQ=255)

                            gcgcTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTg                                                                                                                           <  2:2707512/150‑1 (MQ=255)

                            gcgcTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTg                                                                                                                           <  1:62847/150‑1 (MQ=255)

                                  ggTGCGTTTACCTCCGTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATAc                                                                                                                     <  2:4119976/150‑1 (MQ=255)

                                      cgattACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACg                                                                                                                 <  2:1003442/147‑1 (MQ=255)

                                      cGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACg                                                                                                                 <  2:247866/150‑1 (MQ=255)

                                       gTTTACCTCCTTCCCGGTGATGATGAGCTGGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACgg                                                                                                                <  2:551854/150‑1 (MQ=255)

                                        tttACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGt                                                                                                               <  1:3626086/150‑1 (MQ=255)

                                           aCCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCtg                                                                                                            >  2:1809102/1‑150 (MQ=255)

                                             ctcctTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCtggt                                                                                                          >  2:2398723/1‑150 (MQ=255)

                                                 ttCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCg                                                                                                      <  2:1543031/150‑1 (MQ=255)

                                                    ccGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGGTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATg                                                                                                   >  1:3158030/1‑150 (MQ=255)

                                                        tgatgatgaGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCaa                                                                                               >  1:3002620/1‑150 (MQ=255)

                                                        tgatgatgaGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCaa                                                                                               >  2:2375152/1‑150 (MQ=255)

                                                         gatgatgaGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAg                                                                                              >  1:3763198/1‑150 (MQ=255)

                                                         gatgatgaGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAg                                                                                              <  1:2415180/150‑1 (MQ=255)

                                                           tgatgaGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGcc                                                                                            >  1:95254/1‑150 (MQ=255)

                                                           tgatgaGCTTGCTGGGACTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGcc                                                                                            <  1:1682784/150‑1 (MQ=255)

                                                            gatgaGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCt                                                                                           <  2:1887662/150‑1 (MQ=255)

                                                              tgaGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATGTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGa                                                                                         <  2:2896432/150‑1 (MQ=255)

                                                                    tGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCg                                                                                   <  2:346693/150‑1 (MQ=255)

                                                                     gCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGa                                                                                  >  1:3075387/1‑150 (MQ=255)

                                                                       tGGGGCTGGCGGTCATCTTCGGCCTGGAGAGGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAg                                                                                >  1:2382714/1‑150 (MQ=255)

                                                                       tGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAg                                                                                >  1:329972/1‑150 (MQ=255)

                                                                         gggCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGAt                                                                              <  2:1368505/150‑1 (MQ=255)

                                                                           gCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATGTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATgg                                                                            <  2:564890/150‑1 (MQ=255)

                                                                             tGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTa                                                                          >  1:1046514/1‑150 (MQ=255)

                                                                              ggCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGAGTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTaa                                                                         >  1:1876167/1‑150 (MQ=255)

                                                                                 ggTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGtc                                                                      <  1:237491/150‑1 (MQ=255)

                                                                                   tCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGtctc                                                                    >  1:4402905/1‑150 (MQ=255)

                                                                                    cATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTctct                                                                   <  2:3967813/150‑1 (MQ=255)

                                                                                         tCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGAttt                                                              <  1:4215297/150‑1 (MQ=255)

                                                                                          cGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGAtttt                                                             >  1:387923/1‑150 (MQ=255)

                                                                                          cGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGAtttt                                                             >  1:914934/1‑150 (MQ=255)

                                                                                          cGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGAACATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGGCTGGTGGCGATGCCAAGCCTGACTGGCGAAGAAGGTAAGTCTCTGAttta                                                             >  1:4439172/1‑149 (MQ=255)

                                                                                             ccTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAg                                                          >  1:3564728/1‑150 (MQ=255)

                                                                                               tGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCTTCGGCTTAATCTTTGACCCAGCTGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGcc                                                        <  1:198650/150‑1 (MQ=255)

                                                                                               tGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGcc                                                        <  1:1395202/150‑1 (MQ=255)

                                                                                                 gagaAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTc                                                      <  2:1099151/150‑1 (MQ=255)

                                                                                                 gagaAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTc                                                      <  2:2990269/150‑1 (MQ=255)

                                                                                                 gagaAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTc                                                      >  1:3831929/1‑150 (MQ=255)

                                                                                                  agaAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCg                                                     >  2:4344550/1‑150 (MQ=255)

                                                                                                  agaAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCg                                                     >  2:2330904/1‑150 (MQ=255)

                                                                                                  agaAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCg                                                     <  2:3142295/150‑1 (MQ=255)

                                                                                                  agaAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCg                                                     >  1:3598487/1‑150 (MQ=255)

                                                                                                  agaAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCg                                                     >  1:3598582/1‑150 (MQ=255)

                                                                                                       tgtCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGAtatt                                                >  1:3850993/1‑150 (MQ=255)

                                                                                                          cgcgTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATg                                             >  2:3640174/1‑150 (MQ=255)

                                                                                                            cgTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATggg                                           >  2:1950785/1‑150 (MQ=255)

                                                                                                             gTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGt                                          >  1:1786801/1‑150 (MQ=255)

                                                                                                              tACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTg                                         <  1:1291290/150‑1 (MQ=255)

                                                                                                              tACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTg                                         <  2:2142314/150‑1 (MQ=255)

                                                                                                                cccGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAATCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCa                                       >  2:253080/1‑150 (MQ=255)

                                                                                                                cccGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCTATATTATGGGTGCa                                       >  2:3479207/1‑150 (MQ=255)

                                                                                                                 ccGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCAc                                      <  2:1366001/150‑1 (MQ=255)

                                                                                                                 ccGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCAc                                      >  1:401454/1‑150 (MQ=255)

                                                                                                                  cggcggGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACt                                     >  2:1224287/1‑150 (MQ=255)

                                                                                                                   ggcggGATCTTGTTGGTGATTATTGCAATTTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTc                                    >  2:3532509/1‑150 (MQ=255)

                                                                                                                      gggATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAg                                 >  1:1103931/1‑150 (MQ=255)

                                                                                                                      gggATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAg                                 >  1:523914/1‑150 (MQ=255)

                                                                                                                      gggATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAg                                 >  1:4267318/1‑150 (MQ=255)

                                                                                                                      gggATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAg                                 >  1:2241364/1‑150 (MQ=255)

                                                                                                                         aTCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCa                              <  2:3026113/150‑1 (MQ=255)

                                                                                                                            ttgttgGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACt                           <  1:952915/150‑1 (MQ=255)

                                                                                                                            ttgttgGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACt                           >  2:4573809/1‑150 (MQ=255)

                                                                                                                            ttgttgGTGATTATTGCAATTTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACt                           <  2:3727789/150‑1 (MQ=255)

                                                                                                                              gttgGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGt                         <  2:3789672/150‑1 (MQ=255)

                                                                                                                                tgGTGATTATTGCAATTTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTAc                       <  2:326199/150‑1 (MQ=255)

                                                                                                                                 ggTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATCTTATGGGTGCACTCCAGCCAACTGTACt                      >  2:3631389/1‑150 (MQ=255)

                                                                                                                                 ggTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACt                      <  2:1307995/150‑1 (MQ=255)

                                                                                                                                 ggTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACt                      <  1:1597180/150‑1 (MQ=255)

                                                                                                                                  gTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACtt                     <  1:1167661/150‑1 (MQ=255)

                                                                                                                                  gTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACtt                     <  1:2830166/150‑1 (MQ=255)

                                                                                                                                    gATTATTGCAATTTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTcc                   >  2:2327660/1‑150 (MQ=255)

                                                                                                                                     attattGCAATTTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCg                  >  1:36513/1‑150 (MQ=255)

                                                                                                                                      ttattGCAATTTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGa                 <  1:2339335/150‑1 (MQ=255)

                                                                                                                                        attGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAgt               <  2:3867935/150‑1 (MQ=255)

                                                                                                                                          tGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAgtgt             <  1:2639137/150‑1 (MQ=255)

                                                                                                                                            cAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTAc           >  2:3584613/1‑150 (MQ=255)

                                                                                                                                            cAATGTCGACCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTAc           <  2:168211/150‑1 (MQ=255)

                                                                                                                                               tttCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTgg        <  1:2398723/150‑1 (MQ=255)

                                                                                                                                                  cGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCAt     >  1:3014803/1‑150 (MQ=255)

                                                                                                                                                   gATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCAtt    <  1:3864348/150‑1 (MQ=255)

                                                                                                                                                   gATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCAtt    <  1:1509998/150‑1 (MQ=255)

                                                                                                                                                   gATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCAtt    >  2:785381/1‑150 (MQ=255)

                                                                                                                                                    atcatcGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTg   >  1:508303/1‑150 (MQ=255)

                                                                                                                                                     tcatcGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCAGTgg  >  2:4011106/1‑150 (MQ=255)

CAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGGCGGGATCTTGTTGGTGATTATTGCAATTTCGATCATCGGCTTAATCTTTGACCCAGCGGTGAAATACCACGGTCTGGTGGCGATGCCAAGCCTGACTGGCGAAGATGGTAAGTCTCTGATTTTCAGCCTCGATATTATGGGTGCACTCCAGCCAACTGTACTTCCGAGTGTACTGGCATTGG  >  CP009273/3020992‑3021290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`